(Circular) (Six-base) MAPSORT of: pFN21AB3720.seq Check: 923 from: 1 to: 5677 pFN21AB3720 5677 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3487 278 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3020 1937 Size: 1083 4594 Acc65I G'GTAC_C Cuts at: 2998 2998 Size: 5677 AclI AA'CG_TT Cuts at: 1563 3804 4177 1563 Size: 2241 373 3063 Fragments arranged by size: 3063 2241 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3740 5124 5392 1632 Size: 2108 1384 268 1917 Fragments arranged by size: 2108 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5480 AflIII A'CryG_T Cuts at: 1203 4576 5553 1203 Size: 3373 977 1327 Fragments arranged by size: 3373 1327 977 AgeI A'CCGG_T Cuts at: 4508 4508 Size: 5677 AhdI GACnn_n'nnGTC Cuts at: 4406 4406 Size: 5677 AleI CACnn'nnGTG Cuts at: 1166 2169 2750 1166 Size: 1003 581 4093 Fragments arranged by size: 4093 1003 581 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2175 1184 Size: 991 4686 AlwNI CAG_nnn'CTG Cuts at: 2563 3420 4992 2563 Size: 857 1572 3248 Fragments arranged by size: 3248 1572 857 ApaI G_GGCC'C Cuts at: 2947 2947 Size: 5677 ApaLI G'TGCA_C Cuts at: 3734 4890 3734 Size: 1156 4521 ApoI r'AATT_y Cuts at: 1493 2381 2986 3212 3364 5473 1493 Size: 888 605 226 152 2109 1697 Fragments arranged by size: 2109 1697 888 605 226 152 AseI AT'TA_AT Cuts at: 160 1032 4231 160 Size: 872 3199 1606 Fragments arranged by size: 3199 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 AvaI C'yCGr_G Cuts at: 1870 1943 2640 3002 1870 Size: 73 697 362 4545 Fragments arranged by size: 4545 697 362 73 AvrII C'CTAG_G Cuts at: 2963 2963 Size: 5677 BamHI G'GATC_C Cuts at: 3007 3374 3007 Size: 367 5310 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2744 2847 2998 4453 618 Size: 325 217 627 957 103 151 1455 1842 Fragments arranged by size: 1842 1455 957 627 325 217 151 103 BanII G_rGCy'C Cuts at: 1391 2882 2947 2996 1391 Size: 1491 65 49 4072 Fragments arranged by size: 4072 1491 65 49 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4706 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3868 3902 1903 Size: 34 50 34 1847 34 3678 Fragments arranged by size: 3678 1847 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3569 4779 3569 Size: 1210 4467 BclI T'GATC_A Cuts at: 1548 1548 Size: 5677 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2072 2717 4288 Size: 107 122 71 631 605 400 645 1571 Cuts at: 4288 136 Size: 1525 Fragments arranged by size: 1571 1525 645 631 605 400 122 107 71 BglII A'GATC_T Cuts at: 5672 5672 Size: 5677 BlpI GC'TnA_GC Cuts at: 3094 3094 Size: 5677 Bme1580I G_kGCm'C Cuts at: 1790 2090 2620 2641 2747 2802 2947 3738 4894 Size: 300 530 21 106 55 145 791 1156 Cuts at: 4894 1790 Size: 2573 Fragments arranged by size: 2573 1156 791 530 300 145 106 55 21 BmgBI CAC'GTC Cuts at: 1318 2859 1318 Size: 1541 4136 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2350 2909 3408 Size: 416 53 83 399 24 933 559 499 Cuts at: 3408 4366 442 Size: 958 1753 Fragments arranged by size: 1753 958 933 559 499 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2262 2672 1056 Size: 1206 410 4061 Fragments arranged by size: 4061 1206 410 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2188 2303 2757 4337 1239 Size: 949 115 454 1580 2579 Fragments arranged by size: 2579 1580 949 454 115 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3802 4667 4965 3802 Size: 865 298 4514 Fragments arranged by size: 4514 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4340 882 Size: 617 2841 2219 Fragments arranged by size: 2841 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2626 3468 493 Size: 711 1422 842 2702 Fragments arranged by size: 2702 1422 842 711 BsaBI GATnn'nnATC Cuts at: 5671 5671 Size: 5677 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3484 3866 4501 275 Size: 53 83 186 936 1951 382 635 1451 Fragments arranged by size: 1951 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2139 2913 4110 4508 4782 4929 5340 2139 Size: 774 1197 398 274 147 411 2476 Fragments arranged by size: 2476 1197 774 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2474 2504 1178 Size: 30 165 30 1071 30 4351 Fragments arranged by size: 4351 1071 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2228 2256 2720 1186 Size: 1042 28 464 4143 Fragments arranged by size: 4143 1042 464 28 BseYI C'CCAG_C Cuts at: 2549 2567 2599 2940 4880 5376 2549 Size: 18 32 341 1940 496 2850 Fragments arranged by size: 2850 1940 496 341 32 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2145 2212 1163 Size: 573 409 67 4628 Fragments arranged by size: 4628 573 409 67 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3127 3888 4916 1540 Size: 275 186 1126 761 1028 2301 Fragments arranged by size: 2301 1126 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3738 3823 4894 3738 Size: 85 1071 4521 Fragments arranged by size: 4521 1071 85 BsmI GAATG_Cn' Cuts at: 2426 3188 3281 2426 Size: 762 93 4822 Fragments arranged by size: 4822 762 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2620 2641 2747 2802 2882 2947 Size: 399 300 530 21 106 55 80 65 Cuts at: 2947 2996 3738 3823 4894 1391 Size: 49 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 530 399 300 106 85 80 65 55 49 21 BspEI T'CCGG_A Cuts at: 2913 5340 2913 Size: 2427 3250 BspHI T'CATG_A Cuts at: 3566 5296 3566 Size: 1730 3947 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3032 844 Size: 2188 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2485 3564 1394 Size: 429 662 1079 3507 Fragments arranged by size: 3507 1079 662 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2823 4172 4346 65 Size: 1183 1575 1349 174 1396 Fragments arranged by size: 1575 1396 1349 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4321 4508 1700 Size: 110 2511 187 2869 Fragments arranged by size: 2869 2511 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5677 BssHII G'CGCG_C Cuts at: 1925 2016 1925 Size: 91 5586 BssSI C'ACGA_G Cuts at: 3737 4749 3737 Size: 1012 4665 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5677 BstBI TT'CG_AA Cuts at: 4481 4481 Size: 5677 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5677 BstXI CCAn_nnnn'nTGG Cuts at: 2035 2501 2035 Size: 466 5211 BstYI r'GATC_y Cuts at: 1972 3007 3374 3759 3776 5228 5314 5326 5672 Size: 1035 367 385 17 1452 86 12 346 Cuts at: 5672 1972 Size: 1977 Fragments arranged by size: 1977 1452 1035 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2349 2539 2802 513 Size: 552 1284 190 263 3388 Fragments arranged by size: 3388 1284 552 263 190 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2443 487 Size: 683 56 1217 3721 Fragments arranged by size: 3721 1217 683 56 BtsI GCAGTG_nn' Cuts at: 2224 3193 3987 4007 2224 Size: 969 794 20 3894 Fragments arranged by size: 3894 969 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5677 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5642 DraI TTT'AAA Cuts at: 2981 2981 Size: 5677 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4684 809 Size: 507 3368 1802 Fragments arranged by size: 3368 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3124 4013 5642 Size: 54 1425 50 275 18 1294 889 1629 Cuts at: 5642 8 Size: 43 Fragments arranged by size: 1629 1425 1294 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3124 1537 Size: 275 1312 4090 Fragments arranged by size: 4090 1312 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3607 1353 Size: 2254 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4264 4638 4784 387 Size: 1064 138 2675 374 146 1280 Fragments arranged by size: 2675 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2188 2303 2757 3740 4337 5124 5392 Size: 393 556 115 454 983 597 787 268 Cuts at: 5392 1239 Size: 1524 Fragments arranged by size: 1524 983 787 597 556 454 393 268 115 EcoO109I rG'GnC_Cy Cuts at: 2560 2719 2927 2560 Size: 159 208 5310 Fragments arranged by size: 5310 208 159 EcoRI G'AATT_C Cuts at: 2986 3364 2986 Size: 378 5299 FspI TGC'GCA Cuts at: 2294 4183 2294 Size: 1889 3788 HaeII r_GCGC'y Cuts at: 1678 4824 1678 Size: 3146 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2188 2220 2496 2528 4318 Size: 32 135 32 616 32 276 32 1790 Cuts at: 4318 4350 4392 4424 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1790 616 276 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3021 3267 669 Size: 988 281 1083 246 3079 Fragments arranged by size: 3079 1083 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5677 HpaI GTT'AAC Cuts at: 3267 3267 Size: 5677 KpnI G_GTAC'C Cuts at: 3002 3002 Size: 5677 MfeI C'AATT_G Cuts at: 3276 3276 Size: 5677 MluI A'CGCG_T Cuts at: 5553 5553 Size: 5677 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2472 4791 4975 1305 Size: 1167 2319 184 2007 Fragments arranged by size: 2319 2007 1167 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3855 Fragments arranged by size: 3855 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2169 2474 2750 3635 3994 4153 518 Size: 648 1003 305 276 885 359 159 2042 Fragments arranged by size: 2042 1003 885 648 359 305 276 159 MspA1I CmG'CkG Cuts at: 1606 2844 3093 3770 4918 5163 1606 Size: 1238 249 677 1148 245 2120 Fragments arranged by size: 2120 1238 1148 677 249 245 NcoI C'CATG_G Cuts at: 513 1065 2539 513 Size: 552 1474 3651 Fragments arranged by size: 3651 1474 552 NdeI CA'TA_TG Cuts at: 387 2701 2814 387 Size: 2314 113 3250 Fragments arranged by size: 3250 2314 113 NheI G'CTAG_C Cuts at: 1052 2258 2668 1052 Size: 1206 410 4061 Fragments arranged by size: 4061 1206 410 NotI GC'GGCC_GC Cuts at: 3124 3124 Size: 5677 NspI r_CATG'y Cuts at: 1132 1229 3035 4580 1132 Size: 97 1806 1545 2229 Fragments arranged by size: 2229 1806 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5677 PciI A'CATG_T Cuts at: 4576 4576 Size: 5677 PflMI CCAn_nnn'nTGG Cuts at: 1108 2371 1108 Size: 1263 4414 PfoI T'CCnGG_A Cuts at: 2649 2649 Size: 5677 PmeI GTTT'AAAC Cuts at: 2981 2981 Size: 5677 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3746 3778 5259 5291 3746 Size: 32 1481 32 4132 Fragments arranged by size: 4132 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2927 2927 Size: 5677 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5677 PsiI TTA'TAA Cuts at: 3247 3247 Size: 5677 PspOMI G'GGCC_C Cuts at: 2943 2943 Size: 5677 PstI C_TGCA'G Cuts at: 830 2559 2682 3029 830 Size: 1729 123 347 3478 Fragments arranged by size: 3478 1729 347 123 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5677 PvuII CAG'CTG Cuts at: 2844 2844 Size: 5677 SalI G'TCGA_C Cuts at: 1936 3019 1936 Size: 1083 4594 SbfI CC_TGCA'GG Cuts at: 2559 3029 2559 Size: 470 5207 ScaI AGT'ACT Cuts at: 2300 3925 5637 2300 Size: 1625 1712 2340 Fragments arranged by size: 2340 1712 1625 SexAI A'CCwGG_T Cuts at: 2112 2112 Size: 5677 SfcI C'TryA_G Cuts at: 826 1045 2555 2678 2839 3025 4160 4841 5032 Size: 219 1510 123 161 186 1135 681 191 Cuts at: 5032 826 Size: 1471 Fragments arranged by size: 1510 1471 1135 681 219 191 186 161 123 SfiI GGCCn_nnn'nGGCC Cuts at: 2717 2717 Size: 5677 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5677 SmaI CCC'GGG Cuts at: 1872 3004 1872 Size: 1132 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3781 4682 4944 820 Size: 197 926 1838 901 262 1553 Fragments arranged by size: 1838 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3468 493 Size: 2975 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5677 SphI G_CATG'C Cuts at: 1132 3035 1132 Size: 1903 3774 SspI AAT'ATT Cuts at: 5 52 3601 5 Size: 47 3549 2081 Fragments arranged by size: 3549 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5677 StyI C'CwwG_G Cuts at: 513 1065 2386 2539 2748 2963 3116 513 Size: 552 1321 153 209 215 153 3074 Fragments arranged by size: 3074 1321 552 215 209 153 153 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2890 3704 3721 3874 4059 4504 325 Size: 1255 1310 814 17 153 185 445 1498 Fragments arranged by size: 1498 1310 1255 814 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2176 2298 3923 Size: 274 80 33 51 1443 199 122 1625 Cuts at: 3923 5635 96 Size: 1712 138 Fragments arranged by size: 1712 1625 1443 274 199 138 122 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2330 2362 2474 2506 5637 5669 2330 Size: 32 112 32 3131 32 2338 Fragments arranged by size: 3131 2338 112 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5677 XbaI T'CTAG_A Cuts at: 3013 3013 Size: 5677 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2896 2896 Size: 5677 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5677 XmaI C'CCGG_G Cuts at: 1870 3002 1870 Size: 1132 4545 XmnI GAAnn'nnTTC Cuts at: 3806 3806 Size: 5677 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3485 276 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfoI SrfI SwaI