(Circular) (Six-base) MAPSORT of: pFN21AB3705.seq Check: 7760 from: 1 to: 7915 pFN21AB3705 7915 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5725 278 Size: 53 83 186 936 4189 2468 Fragments arranged by size: 4189 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4371 5258 1937 Size: 2434 887 4594 Fragments arranged by size: 4594 2434 887 Acc65I G'GTAC_C Cuts at: 2140 5236 2140 Size: 3096 4819 AclI AA'CG_TT Cuts at: 1563 2695 6042 6415 1563 Size: 1132 3347 373 3063 Fragments arranged by size: 3347 3063 1132 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 1996 2454 3761 3773 5978 7362 7630 1632 Size: 364 458 1307 12 2205 1384 268 1917 Fragments arranged by size: 2205 1917 1384 1307 458 364 268 12 AflII C'TTAA_G Cuts at: 820 1017 5075 820 Size: 197 4058 3660 Fragments arranged by size: 4058 3660 197 AflIII A'CryG_T Cuts at: 1203 2628 6814 7791 1203 Size: 1425 4186 977 1327 Fragments arranged by size: 4186 1425 1327 977 AgeI A'CCGG_T Cuts at: 6746 6746 Size: 7915 AhdI GACnn_n'nnGTC Cuts at: 4279 6644 4279 Size: 2365 5550 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7915 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4074 1184 Size: 2890 5025 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2538 2570 2538 Size: 32 7883 AlwNI CAG_nnn'CTG Cuts at: 3784 5658 7230 3784 Size: 1874 1572 4469 Fragments arranged by size: 4469 1874 1572 ApaLI G'TGCA_C Cuts at: 4049 5972 7128 4049 Size: 1923 1156 4836 Fragments arranged by size: 4836 1923 1156 ApoI r'AATT_y Cuts at: 1493 2411 2444 2465 2923 3161 3485 4703 4834 Size: 918 33 21 458 238 324 1218 131 Cuts at: 4834 5135 5224 5450 5602 7711 1493 Size: 301 89 226 152 2109 1697 Fragments arranged by size: 2109 1697 1218 918 458 324 301 238 226 152 131 89 33 21 AseI AT'TA_AT Cuts at: 160 1032 3171 3264 6469 160 Size: 872 2139 93 3205 1606 Fragments arranged by size: 3205 2139 1606 872 93 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7915 AvaI C'yCGr_G Cuts at: 1870 1943 2145 3813 5240 1870 Size: 73 202 1668 1427 4545 Fragments arranged by size: 4545 1668 1427 202 73 BamHI G'GATC_C Cuts at: 2428 3637 5245 5612 2428 Size: 1209 1608 367 4731 Fragments arranged by size: 4731 1608 1209 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2140 5236 6691 618 Size: 325 217 627 353 3096 1455 1842 Fragments arranged by size: 3096 1842 1455 627 353 325 217 BanII G_rGCy'C Cuts at: 1391 2836 3093 5234 1391 Size: 1445 257 2141 4072 Fragments arranged by size: 4072 2141 1445 257 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2302 3384 928 Size: 971 403 1082 5459 Fragments arranged by size: 5459 1082 971 403 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3678 3712 6106 6140 1903 Size: 34 1741 34 2394 34 3678 Fragments arranged by size: 3678 2394 1741 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5807 7017 5807 Size: 1210 6705 BclI T'GATC_A Cuts at: 1548 2782 3136 1548 Size: 1234 354 6327 Fragments arranged by size: 6327 1234 354 BfrBI ATG'CAT Cuts at: 4231 4970 4231 Size: 739 7176 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2906 4363 6526 Size: 107 122 71 631 605 1234 1457 2163 Cuts at: 6526 136 Size: 1525 Fragments arranged by size: 2163 1525 1457 1234 631 605 122 107 71 BglII A'GATC_T Cuts at: 5210 7910 5210 Size: 2700 5215 BlpI GC'TnA_GC Cuts at: 3771 5332 3771 Size: 1561 6354 Bme1580I G_kGCm'C Cuts at: 1790 3970 4053 4119 5976 7132 1790 Size: 2180 83 66 1857 1156 2573 Fragments arranged by size: 2573 2180 1857 1156 83 66 BmgBI CAC'GTC Cuts at: 1318 2629 1318 Size: 1311 6604 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2127 5646 6604 Size: 416 53 83 399 24 710 3519 958 Cuts at: 6604 442 Size: 1753 Fragments arranged by size: 3519 1753 958 710 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7915 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3387 6575 1239 Size: 2148 3188 2579 Fragments arranged by size: 3188 2579 2148 Bpu10I CC'TnA_GC Cuts at: 5150 5150 Size: 7915 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3779 6040 6905 7203 3779 Size: 2261 865 298 4491 Fragments arranged by size: 4491 2261 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6578 882 Size: 617 5079 2219 Fragments arranged by size: 5079 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5706 493 Size: 711 4502 2702 Fragments arranged by size: 4502 2702 711 BsaBI GATnn'nnATC Cuts at: 2126 4971 7909 2126 Size: 2845 2938 2132 Fragments arranged by size: 2938 2845 2132 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5722 6104 6739 275 Size: 53 83 186 936 4189 382 635 1451 Fragments arranged by size: 4189 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2811 6348 6746 7020 7167 7578 2811 Size: 3537 398 274 147 411 3148 Fragments arranged by size: 3537 3148 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7690 Fragments arranged by size: 7690 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2288 1186 Size: 1102 6813 BseYI C'CCAG_C Cuts at: 7118 7614 7118 Size: 496 7419 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7342 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5365 6126 7154 1540 Size: 275 186 3364 761 1028 2301 Fragments arranged by size: 3364 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3093 3997 4053 4571 5976 6061 7132 3093 Size: 904 56 518 1405 85 1071 3876 Fragments arranged by size: 3876 1405 1071 904 518 85 56 BsmI GAATG_Cn' Cuts at: 2864 4195 5426 5519 2864 Size: 1331 1231 93 5260 Fragments arranged by size: 5260 1331 1231 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2836 3093 3970 3997 4053 4119 4571 Size: 399 1046 257 877 27 56 66 452 Cuts at: 4571 5234 5976 6061 7132 1391 Size: 663 742 85 1071 2174 Fragments arranged by size: 2174 1071 1046 877 742 663 452 399 257 85 66 56 27 BspEI T'CCGG_A Cuts at: 2811 7578 2811 Size: 4767 3148 BspHI T'CATG_A Cuts at: 5804 7534 5804 Size: 1730 6185 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4129 4284 5270 844 Size: 3285 155 986 3489 Fragments arranged by size: 3489 3285 986 155 BsrBI CCG'CTC Cuts at: 1394 1823 5802 1394 Size: 429 3979 3507 Fragments arranged by size: 3979 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4615 6410 6584 65 Size: 1183 3367 1795 174 1396 Fragments arranged by size: 3367 1795 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4944 6559 6746 1700 Size: 110 3134 1615 187 2869 Fragments arranged by size: 3134 2869 1615 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7915 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7915 BssSI C'ACGA_G Cuts at: 5975 6987 5975 Size: 1012 6903 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7915 BstBI TT'CG_AA Cuts at: 2449 6719 2449 Size: 4270 3645 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7915 BstXI CCAn_nnnn'nTGG Cuts at: 2098 2098 Size: 7915 BstYI r'GATC_y Cuts at: 1972 2428 3637 3670 4501 5210 5245 5612 5997 Size: 456 1209 33 831 709 35 367 385 Cuts at: 5997 6014 7466 7552 7564 7910 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1209 831 709 456 385 367 346 86 35 33 17 12 BstZ17I GTA'TAC Cuts at: 4372 4372 Size: 7915 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7363 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7176 Fragments arranged by size: 7176 683 56 BtsI GCAGTG_nn' Cuts at: 3571 3719 5431 6225 6245 3571 Size: 148 1712 794 20 5241 Fragments arranged by size: 5241 1712 794 148 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7915 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7880 DraI TTT'AAA Cuts at: 3349 4170 4242 4417 5219 3349 Size: 821 72 175 802 6045 Fragments arranged by size: 6045 821 802 175 72 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6922 809 Size: 507 5606 1802 Fragments arranged by size: 5606 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4795 4942 5362 Size: 54 1425 50 275 18 2965 147 420 Cuts at: 5362 6251 7880 8 Size: 889 1629 43 Fragments arranged by size: 2965 1629 1425 889 420 275 147 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5362 1537 Size: 275 3550 4090 Fragments arranged by size: 4090 3550 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2946 4808 5006 5093 5845 1353 Size: 1593 1862 198 87 752 3423 Fragments arranged by size: 3423 1862 1593 752 198 87 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4940 6502 6876 7022 387 Size: 1064 138 3351 1562 374 146 1280 Fragments arranged by size: 3351 1562 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3091 3091 Size: 7915 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1996 2454 3387 3761 3773 5978 6575 Size: 393 364 458 933 374 12 2205 597 Cuts at: 6575 7362 7630 1239 Size: 787 268 1524 Fragments arranged by size: 2205 1524 933 787 597 458 393 374 364 268 12 EcoO109I rG'GnC_Cy Cuts at: 3820 3820 Size: 7915 EcoRI G'AATT_C Cuts at: 5224 5602 5224 Size: 378 7537 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2594 4661 2594 Size: 2067 5848 FspI TGC'GCA Cuts at: 2462 6421 2462 Size: 3959 3956 HaeII r_GCGC'y Cuts at: 1678 3785 5150 7062 1678 Size: 2107 1365 1912 2531 Fragments arranged by size: 2531 2107 1912 1365 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2216 2248 3629 3661 4265 Size: 32 135 32 644 32 1381 32 604 Cuts at: 4265 4297 5195 5227 6556 6588 6630 6662 1373 Size: 32 898 32 1329 32 42 32 2626 Fragments arranged by size: 2626 1381 1329 898 644 604 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4449 5028 5259 5505 669 Size: 988 281 2511 579 231 246 3079 Fragments arranged by size: 3079 2511 988 579 281 246 231 HindIII A'AGCT_T Cuts at: 748 748 Size: 7915 HpaI GTT'AAC Cuts at: 5505 5505 Size: 7915 KpnI G_GTAC'C Cuts at: 2144 5240 2144 Size: 3096 4819 MfeI C'AATT_G Cuts at: 3356 4581 5514 3356 Size: 1225 933 5757 Fragments arranged by size: 5757 1225 933 MluI A'CGCG_T Cuts at: 7791 7791 Size: 7915 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2115 4384 7029 7213 1305 Size: 810 2269 2645 184 2007 Fragments arranged by size: 2645 2269 2007 810 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4797 10 Size: 54 1425 343 2965 3128 Fragments arranged by size: 3128 2965 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2096 4124 5873 6232 6391 518 Size: 648 930 2028 1749 359 159 2042 Fragments arranged by size: 2042 2028 1749 930 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2757 3387 3551 4597 5331 6008 7156 7401 Size: 1151 630 164 1046 734 677 1148 245 Cuts at: 7401 1606 Size: 2120 Fragments arranged by size: 2120 1151 1148 1046 734 677 630 245 164 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7363 NdeI CA'TA_TG Cuts at: 387 387 Size: 7915 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7915 NotI GC'GGCC_GC Cuts at: 5362 5362 Size: 7915 NsiI A_TGCA'T Cuts at: 4233 4972 4233 Size: 739 7176 NspI r_CATG'y Cuts at: 1132 1229 2250 3482 5273 6818 1132 Size: 97 1021 1232 1791 1545 2229 Fragments arranged by size: 2229 1791 1545 1232 1021 97 PasI CC'CwG_GG Cuts at: 1348 2357 1348 Size: 1009 6906 PciI A'CATG_T Cuts at: 6814 6814 Size: 7915 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7915 PmeI GTTT'AAAC Cuts at: 5219 5219 Size: 7915 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5984 6016 7497 7529 5984 Size: 32 1481 32 6370 Fragments arranged by size: 6370 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7915 PsiI TTA'TAA Cuts at: 5485 5485 Size: 7915 PstI C_TGCA'G Cuts at: 830 4281 4624 5035 5267 830 Size: 3451 343 411 232 3478 Fragments arranged by size: 3478 3451 411 343 232 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7915 PvuII CAG'CTG Cuts at: 3387 3551 4597 3387 Size: 164 1046 6705 Fragments arranged by size: 6705 1046 164 SacI G_AGCT'C Cuts at: 3093 3093 Size: 7915 SalI G'TCGA_C Cuts at: 1936 5257 1936 Size: 3321 4594 SapI GCTCTTCn'nnn_ Cuts at: 4808 4808 Size: 7915 SbfI CC_TGCA'GG Cuts at: 5267 5267 Size: 7915 ScaI AGT'ACT Cuts at: 6163 7875 6163 Size: 1712 6203 SfcI C'TryA_G Cuts at: 826 1045 3315 3873 4277 4620 5031 5206 5263 Size: 219 2270 558 404 343 411 175 57 Cuts at: 5263 6398 7079 7270 826 Size: 1135 681 191 1471 Fragments arranged by size: 2270 1471 1135 681 558 411 404 343 219 191 175 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7915 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7915 SmaI CCC'GGG Cuts at: 1872 3815 5242 1872 Size: 1943 1427 4545 Fragments arranged by size: 4545 1943 1427 SmlI C'TyrA_G Cuts at: 820 1017 1943 3758 5075 6019 6920 7182 820 Size: 197 926 1815 1317 944 901 262 1553 Fragments arranged by size: 1815 1553 1317 944 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5706 493 Size: 5213 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7915 SphI G_CATG'C Cuts at: 1132 5273 1132 Size: 4141 3774 SspI AAT'ATT Cuts at: 5 52 3175 3378 5839 5 Size: 47 3123 203 2461 2081 Fragments arranged by size: 3123 2461 2081 203 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7915 StyI C'CwwG_G Cuts at: 513 1065 4747 5354 513 Size: 552 3682 607 3074 Fragments arranged by size: 3682 3074 607 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4704 5942 5959 6112 6297 6742 325 Size: 1255 3124 1238 17 153 185 445 1498 Fragments arranged by size: 3124 1498 1255 1238 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4069 6161 7873 Size: 274 80 33 51 1443 2092 2092 1712 Cuts at: 7873 96 Size: 138 Fragments arranged by size: 2092 2092 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7875 7907 7875 Size: 32 7883 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7915 XbaI T'CTAG_A Cuts at: 3974 5251 3974 Size: 1277 6638 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4814 4814 Size: 7915 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7915 XmaI C'CCGG_G Cuts at: 1870 3813 5240 1870 Size: 1943 1427 4545 Fragments arranged by size: 4545 1943 1427 XmnI GAAnn'nnTTC Cuts at: 2971 5000 6044 2971 Size: 2029 1044 4842 Fragments arranged by size: 4842 2029 1044 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5723 276 Size: 53 83 186 936 4189 2468 Fragments arranged by size: 4189 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI