Restriction map for
pFN21AB3702
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccII AccIII AclI
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbrPI
BcgI BclI BglI BlnI BlpI
Bpu1102I BsaAI BsaWI BseAI BsiEI
BsiWI BsoBI BspDI BspEI BsrBI
BsrFI BssHII BstBI BstEII BstPI
BstUI Bsu36I CelII Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII KpnI KspI MluI MroI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcyI 2951 GGCGCC G[GA]^CG[CT]C
AflII 2941 CTTAAG C^TTAAG
ApaI 2525 GGGCCC GGGCC^C
AseI 2578 ATTAAT AT^TAAT
AsnI 2578 ATTAAT AT^TAAT
BbeI 2951 GGCGCC GGCGC^C
BbvCI 1489 GCTGAGG CCTCAGC(-5/-2)
BciVI 511 GGATAC GTATCC(6/5)
BfrI 2941 CTTAAG C^TTAAG
BglII 2222 AGATCT A^GATCT
BpmI 2357 CTGGAG CTGGAG(16/14)
BsaHI 2951 GGCGCC G[GA]^CG[CT]C
BsgI 1478 CTGCAC GTGCAG(16/14)
BsmBI 2613 GAGACG CGTCTC(1/5)
BsmI 1707 GCATTC GAATGC(1/-1)
Bsp1407I 1258 TGTACA T^GTACA
BspHI 156 TCATGA T^CATGA
BspLU11I 1214 ACATGT A^CATGT
BsrGI 1258 TGTACA T^GTACA
Bst1107I 2858 GTATAC GTA^TAC
BstAPI 1480 GCACAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2858 GTATAC GTA^TAC
DraII 2524 AGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 2989 GACTGTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 2524 AGGGCCC [GA]G^G[ACGT]CC[CT]
HaeII 2951 GGCGCC [GA]GCGC^[CT]
Hin1I 2951 GGCGCC G[GA]^CG[CT]C
HpaI 2446 GTTAAC GTT^AAC
KasI 2951 GGCGCC G^GCGCC
MaeII 2919 ACGT A^CGT
MfeI 1943 CAATTG C^AATTG
MunI 1943 CAATTG C^AATTG
NarI 2951 GGCGCC GG^CGCC
PshBI 2578 ATTAAT AT^TAAT
PvuII 1969 CAGCTG CAG^CTG
RcaI 156 TCATGA T^CATGA
SapI 2798 GAAGAGC GCTCTTC(1/4)
SfoI 2951 GGCGCC GGC^GCC
SspBI 1258 TGTACA T^GTACA
StuI 2809 AGGCCT AGG^CCT
XbaI 597 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 33 enzymes) #
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AflIII 1214 ACATGT A^C[GA][CT]GT
2919 ACGTGT
Asp700I 2641 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2696 GAACTAATTC
AspHI 2059 GAGCAC G[AT]GC[AT]^C
2801 GAGCAC
BcnI 2306 CCCGG CC^[GC]GG
2567 CCCGG
BsaBI 112 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2044 GATGAGAATC
BsiHKAI 2059 GAGCAC G[AT]GC[AT]^C
2801 GAGCAC
BsrDI 456 CATTGC GCAATG(2/0)
768 CATTGC
BssSI 959 CACGAG CACGAG(-5/-1)
1354 CTCGTG
BstXI 50 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1387 CCAGAAGCCTGG
CfoI 1092 GCGC GCG^C
2952 GCGC
DraI 106 TTTAAA TTT^AAA
999 TTTAAA
DraIII 1481 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2750 CACAGGGTG
DsaI 43 CCATGG C^C[GA][CT]GG
3080 CCATGG
EarI 1267 CTCTTC CTCTTC(1/4)
2798 GAAGAG
FauI 773 CCCGC CCCGC(4/6)
27 GCGGG
HapII 2307 CCGG C^CGG
2568 CCGG
HgaI 812 GACGC GACGC(5/10)
672 GCGTC
HhaI 1092 GCGC GCG^C
2952 GCGC
HinP1I 1092 GCGC G^CGC
2952 GCGC
HpaII 2307 CCGG C^CGG
2568 CCGG
Ksp632I 1267 CTCTTC CTCTTC(1/4)
2798 GAAGAG
MamI 112 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2044 GATGAGAATC
MslI 157 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
880 CACACAAGTG
MspA1I 16 CAGCGG C[AC]G^C[GT]G
1969 CAGCTG
MspI 2307 CCGG C^CGG
2568 CCGG
NciI 2306 CCCGG CC^[GC]GG
2567 CCCGG
NcoI 43 CCATGG C^CATGG
3080 CCATGG
NspI 1214 ACATGT [GA]CATG^[CT]
2191 GCATGT
PflMI 1612 CCAAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1864 CCAAGTACTGG
PstI 13 CTGCAG CTGCA^G
803 CTGCAG
SpeI 2104 ACTAGT A^CTAGT
2266 ACTAGT
Van91I 1612 CCAAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1864 CCAAGTACTGG
XmnI 2641 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2696 GAACTAATTC
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BalI
BanI BanII BbsI BbvI BfaI
BmyI BpuAI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII