(Circular) (Six-base) MAPSORT of: pFN21AB3699.seq Check: 1612 from: 1 to: 7258 pFN21AB3699 7258 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5068 278 Size: 53 83 186 936 3532 2468 Fragments arranged by size: 3532 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4601 1937 Size: 2664 4594 Acc65I G'GTAC_C Cuts at: 4579 4579 Size: 7258 AclI AA'CG_TT Cuts at: 1563 3229 5385 5758 1563 Size: 1666 2156 373 3063 Fragments arranged by size: 3063 2156 1666 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2852 2861 3350 3390 3425 3728 3793 3794 Size: 1220 9 489 40 35 303 65 1 Cuts at: 3794 4247 4405 5321 6705 6973 1632 Size: 453 158 916 1384 268 1917 Fragments arranged by size: 1917 1384 1220 916 489 453 303 268 158 65 40 35 9 1 AflII C'TTAA_G Cuts at: 820 1017 4139 820 Size: 197 3122 3939 Fragments arranged by size: 3939 3122 197 AflIII A'CryG_T Cuts at: 1203 6157 7134 1203 Size: 4954 977 1327 Fragments arranged by size: 4954 1327 977 AgeI A'CCGG_T Cuts at: 6089 6089 Size: 7258 AhdI GACnn_n'nnGTC Cuts at: 2239 5987 2239 Size: 3748 3510 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7258 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2160 1184 Size: 976 6282 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2385 2417 2771 2803 2385 Size: 32 354 32 6840 Fragments arranged by size: 6840 354 32 32 AlwNI CAG_nnn'CTG Cuts at: 2830 2935 3328 3340 4428 5001 6573 2830 Size: 105 393 12 1088 573 1572 3515 Fragments arranged by size: 3515 1572 1088 573 393 105 12 ApaI G_GGCC'C Cuts at: 2380 3497 2380 Size: 1117 6141 ApaLI G'TGCA_C Cuts at: 5315 6471 5315 Size: 1156 6102 ApoI r'AATT_y Cuts at: 1493 2270 2355 3681 4567 4793 4945 7054 1493 Size: 777 85 1326 886 226 152 2109 1697 Fragments arranged by size: 2109 1697 1326 886 777 226 152 85 AseI AT'TA_AT Cuts at: 160 1032 5812 160 Size: 872 4780 1606 Fragments arranged by size: 4780 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7258 AvaI C'yCGr_G Cuts at: 1870 1943 3167 4011 4583 1870 Size: 73 1224 844 572 4545 Fragments arranged by size: 4545 1224 844 572 73 BamHI G'GATC_C Cuts at: 2302 2448 3601 4588 4955 2302 Size: 146 1153 987 367 4605 Fragments arranged by size: 4605 1153 987 367 146 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3791 4186 4579 6034 618 Size: 325 217 627 2004 395 393 1455 1842 Fragments arranged by size: 2004 1842 1455 627 395 393 325 217 BanII G_rGCy'C Cuts at: 1391 2380 2752 3497 3644 4203 4299 4577 1391 Size: 989 372 745 147 559 96 278 4072 Fragments arranged by size: 4072 989 745 559 372 278 147 96 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2130 2512 2561 3785 928 Size: 971 231 382 49 1224 4401 Fragments arranged by size: 4401 1224 971 382 231 49 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3889 3923 5449 5483 1903 Size: 34 1952 34 1526 34 3678 Fragments arranged by size: 3678 1952 1526 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5150 6360 5150 Size: 1210 6048 BclI T'GATC_A Cuts at: 1548 1548 Size: 7258 BfrBI ATG'CAT Cuts at: 4308 4308 Size: 7258 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3648 5869 136 Size: 107 122 71 631 605 1976 2221 1525 Fragments arranged by size: 2221 1976 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7253 7253 Size: 7258 BlpI GC'TnA_GC Cuts at: 3428 3554 4202 4675 3428 Size: 126 648 473 6011 Fragments arranged by size: 6011 648 473 126 Bme1580I G_kGCm'C Cuts at: 1790 2380 3497 5319 6475 1790 Size: 590 1117 1822 1156 2573 Fragments arranged by size: 2573 1822 1156 1117 590 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7258 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2305 4989 5947 Size: 416 53 83 399 24 888 2684 958 Cuts at: 5947 442 Size: 1753 Fragments arranged by size: 2684 1753 958 888 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7258 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2798 2817 3038 3502 3707 4040 4154 4178 Size: 1559 19 221 464 205 333 114 24 Cuts at: 4178 4289 4301 5918 1239 Size: 111 12 1617 2579 Fragments arranged by size: 2579 1617 1559 464 333 221 205 114 111 24 19 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2990 5383 6248 6546 2990 Size: 2393 865 298 3702 Fragments arranged by size: 3702 2393 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4102 5921 882 Size: 617 2603 1819 2219 Fragments arranged by size: 2603 2219 1819 617 BsaAI yAC'GTr Cuts at: 493 1204 5049 493 Size: 711 3845 2702 Fragments arranged by size: 3845 2702 711 BsaBI GATnn'nnATC Cuts at: 7252 7252 Size: 7258 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5065 5447 6082 275 Size: 53 83 186 936 3532 382 635 1451 Fragments arranged by size: 3532 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3123 5691 6089 6363 6510 6921 3123 Size: 2568 398 274 147 411 3460 Fragments arranged by size: 3460 2568 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2385 2415 2771 2801 1178 Size: 30 165 30 982 30 356 30 5635 Fragments arranged by size: 5635 982 356 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2726 3096 3508 3977 1186 Size: 1540 370 412 469 4467 Fragments arranged by size: 4467 1540 469 412 370 BseYI C'CCAG_C Cuts at: 2724 2964 3490 4514 6461 6957 2724 Size: 240 526 1024 1947 496 3025 Fragments arranged by size: 3025 1947 1024 526 496 240 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2042 2130 3272 1163 Size: 573 306 88 1142 5149 Fragments arranged by size: 5149 1142 573 306 88 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4708 5469 6497 1540 Size: 275 186 2707 761 1028 2301 Fragments arranged by size: 2707 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2752 3855 3876 4203 5319 5404 6475 2752 Size: 1103 21 327 1116 85 1071 3535 Fragments arranged by size: 3535 1116 1103 1071 327 85 21 BsmI GAATG_Cn' Cuts at: 4769 4862 4769 Size: 93 7165 BsmBI CGTCTCn'nnnn_ Cuts at: 4510 4510 Size: 7258 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2380 2752 3497 3644 3855 3876 4203 Size: 399 590 372 745 147 211 21 327 Cuts at: 4203 4299 4577 5319 5404 6475 1391 Size: 96 278 742 85 1071 2174 Fragments arranged by size: 2174 1071 745 742 590 399 372 327 278 211 147 96 85 21 BspEI T'CCGG_A Cuts at: 3123 6921 3123 Size: 3798 3460 BspHI T'CATG_A Cuts at: 5147 6877 5147 Size: 1730 5528 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3845 4613 844 Size: 3001 768 3489 Fragments arranged by size: 3489 3001 768 BsrBI CCG'CTC Cuts at: 1394 1823 5145 1394 Size: 429 3322 3507 Fragments arranged by size: 3507 3322 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3070 5753 5927 65 Size: 1183 1822 2683 174 1396 Fragments arranged by size: 2683 1822 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3765 5902 6089 1700 Size: 110 1955 2137 187 2869 Fragments arranged by size: 2869 2137 1955 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7258 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7258 BssSI C'ACGA_G Cuts at: 5318 6330 5318 Size: 1012 6246 BstAPI GCAn_nnn'nTGC Cuts at: 794 2263 3837 794 Size: 1469 1574 4215 Fragments arranged by size: 4215 1574 1469 BstBI TT'CG_AA Cuts at: 6062 6062 Size: 7258 BstEII G'GTnAC_C Cuts at: 1184 3206 4460 1184 Size: 2022 1254 3982 Fragments arranged by size: 3982 2022 1254 BstXI CCAn_nnnn'nTGG Cuts at: 2893 2893 Size: 7258 BstYI r'GATC_y Cuts at: 1972 2302 2448 2538 3601 3712 4240 4588 4955 Size: 330 146 90 1063 111 528 348 367 Cuts at: 4955 5340 5357 6809 6895 6907 7253 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1063 528 385 367 348 346 330 146 111 90 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2819 2819 Size: 7258 BtgI C'CryG_G Cuts at: 513 1065 2004 2761 4488 513 Size: 552 939 757 1727 3283 Fragments arranged by size: 3283 1727 939 757 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6519 Fragments arranged by size: 6519 683 56 BtsI GCAGTG_nn' Cuts at: 2018 2660 3983 4220 4774 5568 5588 2018 Size: 642 1323 237 554 794 20 3688 Fragments arranged by size: 3688 1323 794 642 554 237 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7258 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7223 DraI TTT'AAA Cuts at: 4562 4562 Size: 7258 DraIII CAC_nnn'GTG Cuts at: 3272 3272 Size: 7258 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6265 809 Size: 507 4949 1802 Fragments arranged by size: 4949 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2218 3529 4477 Size: 54 1425 50 275 18 388 1311 948 Cuts at: 4477 4705 5594 7223 8 Size: 228 889 1629 43 Fragments arranged by size: 1629 1425 1311 948 889 388 275 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4705 1537 Size: 275 2893 4090 Fragments arranged by size: 4090 2893 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5188 1353 Size: 3835 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3929 5845 6219 6365 387 Size: 1064 138 2340 1916 374 146 1280 Fragments arranged by size: 2340 1916 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2750 4201 2750 Size: 1451 5807 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2798 2817 2852 2861 3038 3350 3390 Size: 393 1166 19 35 9 177 312 40 Cuts at: 3390 3425 3502 3707 3728 3793 3794 4040 4154 Size: 35 77 205 21 65 1 246 114 Cuts at: 4154 4178 4247 4289 4301 4405 5321 5918 6705 Size: 24 69 42 12 104 916 597 787 Cuts at: 6705 6973 1239 Size: 268 1524 Fragments arranged by size: 1524 1166 916 787 597 393 312 268 246 205 177 114 104 77 69 65 42 40 35 35 24 21 19 12 9 1 EcoO109I rG'GnC_Cy Cuts at: 2233 2376 3475 3494 4343 2233 Size: 143 1099 19 849 5148 Fragments arranged by size: 5148 1099 849 143 19 EcoRI G'AATT_C Cuts at: 4567 4945 4567 Size: 378 6880 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2165 2165 Size: 7258 FspI TGC'GCA Cuts at: 5764 5764 Size: 7258 HaeII r_GCGC'y Cuts at: 1678 3766 6405 1678 Size: 2088 2639 2531 Fragments arranged by size: 2639 2531 2088 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2225 2257 2353 2385 2426 Size: 32 135 32 653 32 96 32 41 Cuts at: 2426 2458 2523 2548 2555 2580 2985 3017 3586 Size: 32 65 25 7 25 405 32 569 Cuts at: 3586 3618 4145 4177 4244 4276 5899 5931 5973 Size: 32 527 32 67 32 1623 32 42 Cuts at: 5973 6005 1373 Size: 32 2626 Fragments arranged by size: 2626 1623 653 569 527 405 135 96 67 65 42 41 32 32 32 32 32 32 32 32 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 669 1657 1938 4602 4848 669 Size: 988 281 2664 246 3079 Fragments arranged by size: 3079 2664 988 281 246 HindIII A'AGCT_T Cuts at: 748 4020 748 Size: 3272 3986 HpaI GTT'AAC Cuts at: 4848 4848 Size: 7258 KpnI G_GTAC'C Cuts at: 4583 4583 Size: 7258 MfeI C'AATT_G Cuts at: 2381 3393 4857 2381 Size: 1012 1464 4782 Fragments arranged by size: 4782 1464 1012 MluI A'CGCG_T Cuts at: 7134 7134 Size: 7258 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2688 2748 3399 3486 3731 6372 6556 1305 Size: 1383 60 651 87 245 2641 184 2007 Fragments arranged by size: 2641 2007 1383 651 245 184 87 60 MscI TGG'CCA Cuts at: 10 64 1489 1832 4479 10 Size: 54 1425 343 2647 2789 Fragments arranged by size: 2789 2647 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3858 5216 5575 5734 518 Size: 648 2692 1358 359 159 2042 Fragments arranged by size: 2692 2042 1358 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2056 2203 2776 2795 2827 3553 3925 4132 Size: 450 147 573 19 32 726 372 207 Cuts at: 4132 4674 5351 6499 6744 1606 Size: 542 677 1148 245 2120 Fragments arranged by size: 2120 1148 726 677 573 542 450 372 245 207 147 32 19 NaeI GCC'GGC Cuts at: 3767 3767 Size: 7258 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5767 Fragments arranged by size: 5767 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7258 NgoMIV G'CCGG_C Cuts at: 3765 3765 Size: 7258 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7258 NotI GC'GGCC_GC Cuts at: 4705 4705 Size: 7258 NsiI A_TGCA'T Cuts at: 4310 4310 Size: 7258 NspI r_CATG'y Cuts at: 1132 1229 2977 3562 4616 6161 1132 Size: 97 1748 585 1054 1545 2229 Fragments arranged by size: 2229 1748 1545 1054 585 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7258 PciI A'CATG_T Cuts at: 6157 6157 Size: 7258 PflMI CCAn_nnn'nTGG Cuts at: 1108 2194 2767 1108 Size: 1086 573 5599 Fragments arranged by size: 5599 1086 573 PfoI T'CCnGG_A Cuts at: 2444 2451 3054 4056 2444 Size: 7 603 1002 5646 Fragments arranged by size: 5646 1002 603 7 PmeI GTTT'AAAC Cuts at: 4562 4562 Size: 7258 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2385 2417 2771 2803 3374 3406 5327 5359 6840 Size: 32 354 32 571 32 1921 32 1481 Cuts at: 6840 6872 2385 Size: 32 2771 Fragments arranged by size: 2771 1921 1481 571 354 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2233 4343 2233 Size: 2110 5148 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7258 PsiI TTA'TAA Cuts at: 4828 4828 Size: 7258 PspOMI G'GGCC_C Cuts at: 2376 3493 2376 Size: 1117 6141 PstI C_TGCA'G Cuts at: 830 2028 2256 2604 3915 4610 830 Size: 1198 228 348 1311 695 3478 Fragments arranged by size: 3478 1311 1198 695 348 228 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7258 PvuII CAG'CTG Cuts at: 2056 2203 2776 2795 2827 3553 2056 Size: 147 573 19 32 726 5761 Fragments arranged by size: 5761 726 573 147 32 19 SacI G_AGCT'C Cuts at: 2752 4203 2752 Size: 1451 5807 SalI G'TCGA_C Cuts at: 1936 4600 1936 Size: 2664 4594 SbfI CC_TGCA'GG Cuts at: 4610 4610 Size: 7258 ScaI AGT'ACT Cuts at: 2105 5506 7218 2105 Size: 3401 1712 2145 Fragments arranged by size: 3401 2145 1712 SfcI C'TryA_G Cuts at: 826 1045 2024 2252 2600 2927 3589 3911 4606 Size: 219 979 228 348 327 662 322 695 Cuts at: 4606 5741 6422 6613 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 979 695 681 662 348 327 322 228 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7258 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7258 SmaI CCC'GGG Cuts at: 1872 4013 4585 1872 Size: 2141 572 4545 Fragments arranged by size: 4545 2141 572 SmlI C'TyrA_G Cuts at: 820 1017 1943 3005 4139 5362 6263 6525 820 Size: 197 926 1062 1134 1223 901 262 1553 Fragments arranged by size: 1553 1223 1134 1062 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5049 493 Size: 4556 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7258 SphI G_CATG'C Cuts at: 1132 2977 3562 4616 1132 Size: 1845 585 1054 3774 Fragments arranged by size: 3774 1845 1054 585 SspI AAT'ATT Cuts at: 5 52 5182 5 Size: 47 5130 2081 Fragments arranged by size: 5130 2081 47 StuI AGG'CCT Cuts at: 1517 3537 1517 Size: 2020 5238 StyI C'CwwG_G Cuts at: 513 1065 2004 2166 3471 4339 4697 513 Size: 552 939 162 1305 868 358 3074 Fragments arranged by size: 3074 1305 939 868 552 358 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3835 4017 5285 5302 5455 5640 6085 Size: 1255 2255 182 1268 17 153 185 445 Cuts at: 6085 325 Size: 1498 Fragments arranged by size: 2255 1498 1268 1255 445 185 182 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2103 5504 7216 Size: 274 80 33 51 1443 126 3401 1712 Cuts at: 7216 96 Size: 138 Fragments arranged by size: 3401 1712 1443 274 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3975 4007 7218 7250 3975 Size: 32 3211 32 3983 Fragments arranged by size: 3983 3211 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3784 1316 Size: 2468 4790 XbaI T'CTAG_A Cuts at: 4594 4594 Size: 7258 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3612 3666 4306 3612 Size: 54 640 6564 Fragments arranged by size: 6564 640 54 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7258 XmaI C'CCGG_G Cuts at: 1870 4011 4583 1870 Size: 2141 572 4545 Fragments arranged by size: 4545 2141 572 XmnI GAAnn'nnTTC Cuts at: 5387 5387 Size: 7258 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5066 276 Size: 53 83 186 936 3532 2468 Fragments arranged by size: 3532 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstZ17I EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI