Restriction map for
pFN21AB3699
(Linear) (Six-base) MAPSORT of: KIBB3699.seq Check: 9879 from: 1 to: 2553
KIBB3699.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AclI AA'CG_TT
Cuts at: 0 1224 2553
Size: 1224 1329
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 856 1345 1385 1420 1723 1788 1789
Size: 847 9 489 40 35 303 65 1
Cuts at: 1789 2242 2400 2553
Size: 453 158 153
Fragments arranged by size:
847 489 453 303 158 153 65 40
35 9 1
AflII C'TTAA_G
Cuts at: 0 2134 2553
Size: 2134 419
AhdI GACnn_n'nnGTC
Cuts at: 0 234 2553
Size: 234 2319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 155 2553
Size: 155 2398
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 380 412 766 798 2553
Size: 380 32 354 32 1755
Fragments arranged by size:
1755 380 354 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 825 930 1323 1335 2423 2553
Size: 825 105 393 12 1088 130
Fragments arranged by size:
1088 825 393 130 105 12
ApaI G_GGCC'C
Cuts at: 0 375 1492 2553
Size: 375 1117 1061
Fragments arranged by size:
1117 1061 375
ApoI r'AATT_y
Cuts at: 0 265 350 1676 2553
Size: 265 85 1326 877
Fragments arranged by size:
1326 877 265 85
AvaI C'yCGr_G
Cuts at: 0 1162 2006 2553
Size: 1162 844 547
Fragments arranged by size:
1162 844 547
BamHI G'GATC_C
Cuts at: 0 297 443 1596 2553
Size: 297 146 1153 957
Fragments arranged by size:
1153 957 297 146
BanI G'GyrC_C
Cuts at: 0 1786 2181 2553
Size: 1786 395 372
Fragments arranged by size:
1786 395 372
BanII G_rGCy'C
Cuts at: 0 375 747 1492 1639 2198 2294 2553
Size: 375 372 745 147 559 96 259
Fragments arranged by size:
745 559 375 372 259 147 96
BbsI GAAGACnn'nnnn_
Cuts at: 0 125 507 556 1780 2553
Size: 125 382 49 1224 773
Fragments arranged by size:
1224 773 382 125 49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1884 1918 2553
Size: 1884 34 635
Fragments arranged by size:
1884 635 34
BfrBI ATG'CAT
Cuts at: 0 2303 2553
Size: 2303 250
BglI GCCn_nnn'nGGC
Cuts at: 0 1643 2553
Size: 1643 910
BlpI GC'TnA_GC
Cuts at: 0 1423 1549 2197 2553
Size: 1423 126 648 356
Fragments arranged by size:
1423 648 356 126
Bme1580I G_kGCm'C
Cuts at: 0 375 1492 2553
Size: 375 1117 1061
Fragments arranged by size:
1117 1061 375
BmrI ACTGGGnnnn_n'
Cuts at: 0 300 2553
Size: 300 2253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 812 1033 1497 1702 2035 2149 2173
Size: 793 19 221 464 205 333 114 24
Cuts at: 2173 2284 2296 2553
Size: 111 12 257
Fragments arranged by size:
793 464 333 257 221 205 114 111
24 19 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 985 2553
Size: 985 1568
BsaI GGTCTCn'nnnn_
Cuts at: 0 2097 2553
Size: 2097 456
BsaWI w'CCGG_w
Cuts at: 0 1118 2553
Size: 1118 1435
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 380 410 766 796 2553
Size: 380 30 356 30 1757
Fragments arranged by size:
1757 380 356 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 721 1091 1503 1972 2553
Size: 721 370 412 469 581
Fragments arranged by size:
721 581 469 412 370
BseYI C'CCAG_C
Cuts at: 0 719 959 1485 2509 2553
Size: 719 240 526 1024 44
Fragments arranged by size:
1024 719 526 240 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 125 1267 2553
Size: 37 88 1142 1286
Fragments arranged by size:
1286 1142 88 37
BsiHKAI G_wGCw'C
Cuts at: 0 747 1850 1871 2198 2553
Size: 747 1103 21 327 355
Fragments arranged by size:
1103 747 355 327 21
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2505 2553
Size: 2505 48
Bsp1286I G_dGCh'C
Cuts at: 0 375 747 1492 1639 1850 1871 2198 2294
Size: 375 372 745 147 211 21 327 96
Cuts at: 2294 2553
Size: 259
Fragments arranged by size:
745 375 372 327 259 211 147 96
21
BspEI T'CCGG_A
Cuts at: 0 1118 2553
Size: 1118 1435
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1840 2553
Size: 1840 713
BsrDI GCAATG_nn'
Cuts at: 0 1065 2553
Size: 1065 1488
BsrFI r'CCGG_y
Cuts at: 0 1760 2553
Size: 1760 793
BstAPI GCAn_nnn'nTGC
Cuts at: 0 258 1832 2553
Size: 258 1574 721
Fragments arranged by size:
1574 721 258
BstEII G'GTnAC_C
Cuts at: 0 1201 2455 2553
Size: 1201 1254 98
Fragments arranged by size:
1254 1201 98
BstXI CCAn_nnnn'nTGG
Cuts at: 0 888 2553
Size: 888 1665
BstYI r'GATC_y
Cuts at: 0 297 443 533 1596 1707 2235 2553
Size: 297 146 90 1063 111 528 318
Fragments arranged by size:
1063 528 318 297 146 111 90
Bsu36I CC'TnA_GG
Cuts at: 0 814 2553
Size: 814 1739
BtgI C'CryG_G
Cuts at: 0 756 2483 2553
Size: 756 1727 70
Fragments arranged by size:
1727 756 70
BtsI GCAGTG_nn'
Cuts at: 0 13 655 1978 2215 2553
Size: 13 642 1323 237 338
Fragments arranged by size:
1323 642 338 237 13
DraIII CAC_nnn'GTG
Cuts at: 0 1267 2553
Size: 1267 1286
EaeI y'GGCC_r
Cuts at: 0 213 1524 2472 2553
Size: 213 1311 948 81
Fragments arranged by size:
1311 948 213 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1924 2553
Size: 1924 629
EcoICRI GAG'CTC
Cuts at: 0 745 2196 2553
Size: 745 1451 357
Fragments arranged by size:
1451 745 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 812 847 856 1033 1345 1385 1420
Size: 793 19 35 9 177 312 40 35
Cuts at: 1420 1497 1702 1723 1788 1789 2035 2149 2173
Size: 77 205 21 65 1 246 114 24
Cuts at: 2173 2242 2284 2296 2400 2553
Size: 69 42 12 104 153
Fragments arranged by size:
793 312 246 205 177 153 114 104
77 69 65 42 40 35 35 24
21 19 12 9 1
EcoO109I rG'GnC_Cy
Cuts at: 0 228 371 1470 1489 2338 2553
Size: 228 143 1099 19 849 215
Fragments arranged by size:
1099 849 228 215 143 19
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 160 2553
Size: 160 2393
HaeII r_GCGC'y
Cuts at: 0 1761 2553
Size: 1761 792
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 348 380 421 453 518 543
Size: 220 32 96 32 41 32 65 25
Cuts at: 543 550 575 980 1012 1581 1613 2140 2172
Size: 7 25 405 32 569 32 527 32
Cuts at: 2172 2239 2271 2553
Size: 67 32 282
Fragments arranged by size:
569 527 405 282 220 96 67 65
41 32 32 32 32 32 32 32
25 25 7
HindIII A'AGCT_T
Cuts at: 0 2015 2553
Size: 2015 538
MfeI C'AATT_G
Cuts at: 0 376 1388 2553
Size: 376 1012 1165
Fragments arranged by size:
1165 1012 376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 743 1394 1481 1726 2553
Size: 683 60 651 87 245 827
Fragments arranged by size:
827 683 651 245 87 60
MscI TGG'CCA
Cuts at: 0 2474 2553
Size: 2474 79
MslI CAynn'nnrTG
Cuts at: 0 1853 2553
Size: 1853 700
MspA1I CmG'CkG
Cuts at: 0 51 198 771 790 822 1548 1920 2127
Size: 51 147 573 19 32 726 372 207
Cuts at: 2127 2553
Size: 426
Fragments arranged by size:
726 573 426 372 207 147 51 32
19
NaeI GCC'GGC
Cuts at: 0 1762 2553
Size: 1762 791
NgoMIV G'CCGG_C
Cuts at: 0 1760 2553
Size: 1760 793
NsiI A_TGCA'T
Cuts at: 0 2305 2553
Size: 2305 248
NspI r_CATG'y
Cuts at: 0 972 1557 2553
Size: 972 585 996
Fragments arranged by size:
996 972 585
PflMI CCAn_nnn'nTGG
Cuts at: 0 189 762 2553
Size: 189 573 1791
Fragments arranged by size:
1791 573 189
PfoI T'CCnGG_A
Cuts at: 0 439 446 1049 2051 2553
Size: 439 7 603 1002 502
Fragments arranged by size:
1002 603 502 439 7
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 380 412 766 798 1369 1401 2553
Size: 380 32 354 32 571 32 1152
Fragments arranged by size:
1152 571 380 354 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 228 2338 2553
Size: 228 2110 215
Fragments arranged by size:
2110 228 215
PspOMI G'GGCC_C
Cuts at: 0 371 1488 2553
Size: 371 1117 1065
Fragments arranged by size:
1117 1065 371
PstI C_TGCA'G
Cuts at: 0 23 251 599 1910 2553
Size: 23 228 348 1311 643
Fragments arranged by size:
1311 643 348 228 23
PvuII CAG'CTG
Cuts at: 0 51 198 771 790 822 1548 2553
Size: 51 147 573 19 32 726 1005
Fragments arranged by size:
1005 726 573 147 51 32 19
SacI G_AGCT'C
Cuts at: 0 747 2198 2553
Size: 747 1451 355
Fragments arranged by size:
1451 747 355
ScaI AGT'ACT
Cuts at: 0 100 2553
Size: 100 2453
SfcI C'TryA_G
Cuts at: 0 19 247 595 922 1584 1906 2553
Size: 19 228 348 327 662 322 647
Fragments arranged by size:
662 647 348 327 322 228 19
SmaI CCC'GGG
Cuts at: 0 2008 2553
Size: 2008 545
SmlI C'TyrA_G
Cuts at: 0 1000 2134 2553
Size: 1000 1134 419
Fragments arranged by size:
1134 1000 419
SphI G_CATG'C
Cuts at: 0 972 1557 2553
Size: 972 585 996
Fragments arranged by size:
996 972 585
StuI AGG'CCT
Cuts at: 0 1532 2553
Size: 1532 1021
StyI C'CwwG_G
Cuts at: 0 161 1466 2334 2553
Size: 161 1305 868 219
Fragments arranged by size:
1305 868 219 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1830 2012 2553
Size: 1830 182 541
Fragments arranged by size:
1830 541 182
TatI w'GTAC_w
Cuts at: 0 98 2553
Size: 98 2455
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1970 2002 2553
Size: 1970 32 551
Fragments arranged by size:
1970 551 32
Tth111I GACn'n_nGTC
Cuts at: 0 1779 2553
Size: 1779 774
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1607 1661 2301 2553
Size: 1607 54 640 252
Fragments arranged by size:
1607 640 252 54
XmaI C'CCGG_G
Cuts at: 0 2006 2553
Size: 2006 547
Enzymes that do cut:
AclI AcuI AflII AhdI AlfI AloI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbsI BcgI BfrBI
BglI BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EciI EcoICRI Eco57MI EcoO109I
FalI HaeII Hin4I HindIII MfeI MmeI MscI MslI
MspA1I NaeI NgoMIV NsiI NspI PflMI PfoI PpiI
PpuMI PspOMI PstI PvuII SacI ScaI SfcI SmaI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflIII AgeI AleI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI
BciVI BclI BglII BmgBI BmtI BplI Bpu10I BsaAI
BsaBI BsaHI BsiEI BsiWI BsmI BspHI BsrBI BsrGI
BssHII BssSI BstBI BstZ17I BtgZI ClaI CspCI DraI
DrdI EagI EarI EcoNI EcoRI EcoRV FseI FspI
FspAI HincII HpaI KasI KpnI MluI NarI NcoI
NdeI NheI NotI NruI PacI PasI PciI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI XbaI XhoI
XmnI ZraI