(Circular) (Six-base) MAPSORT of: pFN21AB3682.seq Check: 628 from: 1 to: 6961 pFN21AB3682 6961 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3237 3723 3237 Size: 486 6475 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4771 278 Size: 53 83 186 936 3235 2468 Fragments arranged by size: 3235 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4304 1937 Size: 2367 4594 Acc65I G'GTAC_C Cuts at: 4282 4282 Size: 6961 AclI AA'CG_TT Cuts at: 1563 5088 5461 1563 Size: 3525 373 3063 Fragments arranged by size: 3525 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2015 2264 2357 2911 3312 4022 4145 4161 Size: 383 249 93 554 401 710 123 16 Cuts at: 4161 5024 6408 6676 1632 Size: 863 1384 268 1917 Fragments arranged by size: 1917 1384 863 710 554 401 383 268 249 123 93 16 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6764 AflIII A'CryG_T Cuts at: 1203 3570 5860 6837 1203 Size: 2367 2290 977 1327 Fragments arranged by size: 2367 2290 1327 977 AgeI A'CCGG_T Cuts at: 5792 5792 Size: 6961 AhdI GACnn_n'nnGTC Cuts at: 2191 4210 5690 2191 Size: 2019 1480 3462 Fragments arranged by size: 3462 2019 1480 AleI CACnn'nnGTG Cuts at: 1166 3173 3857 1166 Size: 2007 684 4270 Fragments arranged by size: 4270 2007 684 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2555 1184 Size: 1371 5590 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3876 3908 3876 Size: 32 6929 AlwNI CAG_nnn'CTG Cuts at: 2110 4704 6276 2110 Size: 2594 1572 2795 Fragments arranged by size: 2795 2594 1572 ApaLI G'TGCA_C Cuts at: 3486 5018 6174 3486 Size: 1532 1156 4273 Fragments arranged by size: 4273 1532 1156 ApoI r'AATT_y Cuts at: 1493 2048 2352 3218 3661 4235 4270 4496 4648 Size: 555 304 866 443 574 35 226 152 Cuts at: 4648 6757 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 866 574 555 443 304 226 152 35 AseI AT'TA_AT Cuts at: 160 1032 2706 5515 160 Size: 872 1674 2809 1606 Fragments arranged by size: 2809 1674 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6961 AvaI C'yCGr_G Cuts at: 1870 1943 3353 3403 3831 4286 1870 Size: 73 1410 50 428 455 4545 Fragments arranged by size: 4545 1410 455 428 73 50 BamHI G'GATC_C Cuts at: 4291 4658 4291 Size: 367 6594 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3225 3257 4282 5737 618 Size: 325 217 627 1438 32 1025 1455 1842 Fragments arranged by size: 1842 1455 1438 1025 627 325 217 32 BanII G_rGCy'C Cuts at: 1391 2957 3499 3830 4229 4280 1391 Size: 1566 542 331 399 51 4072 Fragments arranged by size: 4072 1566 542 399 331 51 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2304 3023 928 Size: 971 405 719 4866 Fragments arranged by size: 4866 971 719 405 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2810 2844 3349 3383 5152 5186 1903 Size: 34 873 34 505 34 1769 34 3678 Fragments arranged by size: 3678 1769 873 505 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4023 4853 6063 4023 Size: 830 1210 4921 Fragments arranged by size: 4921 1210 830 BclI T'GATC_A Cuts at: 1548 2206 2319 2581 1548 Size: 658 113 262 5928 Fragments arranged by size: 5928 658 262 113 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5572 136 Size: 107 122 71 631 605 3900 1525 Fragments arranged by size: 3900 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6956 6956 Size: 6961 BlpI GC'TnA_GC Cuts at: 3498 4378 3498 Size: 880 6081 Bme1580I G_kGCm'C Cuts at: 1790 3164 3490 3778 5022 6178 1790 Size: 1374 326 288 1244 1156 2573 Fragments arranged by size: 2573 1374 1244 1156 326 288 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6961 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2146 4692 5650 Size: 416 53 83 399 24 729 2546 958 Cuts at: 5650 442 Size: 1753 Fragments arranged by size: 2546 1753 958 729 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6961 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3319 3379 3319 Size: 60 6901 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 4039 5621 1239 Size: 836 1964 1582 2579 Fragments arranged by size: 2579 1964 1582 836 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2591 5086 5951 6249 2591 Size: 2495 865 298 3303 Fragments arranged by size: 3303 2495 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2552 3554 3620 5624 882 Size: 617 1053 1002 66 2004 2219 Fragments arranged by size: 2219 2004 1053 1002 617 66 BsaAI yAC'GTr Cuts at: 493 1204 4752 493 Size: 711 3548 2702 Fragments arranged by size: 3548 2702 711 BsaBI GATnn'nnATC Cuts at: 6955 6955 Size: 6961 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4768 5150 5785 275 Size: 53 83 186 936 3235 382 635 1451 Fragments arranged by size: 3235 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5394 5792 6066 6213 6624 5394 Size: 398 274 147 411 5731 Fragments arranged by size: 5731 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3702 3732 1178 Size: 30 165 30 2299 30 4407 Fragments arranged by size: 4407 2299 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3203 4211 1186 Size: 2017 1008 3936 Fragments arranged by size: 3936 2017 1008 BseYI C'CCAG_C Cuts at: 6164 6660 6164 Size: 496 6465 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6388 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4411 5172 6200 1540 Size: 275 186 2410 761 1028 2301 Fragments arranged by size: 2410 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2145 2408 2957 3490 3830 5022 5107 6178 2145 Size: 263 549 533 340 1192 85 1071 2928 Fragments arranged by size: 2928 1192 1071 549 533 340 263 85 BsmI GAATG_Cn' Cuts at: 2701 4472 4565 2701 Size: 1771 93 5097 Fragments arranged by size: 5097 1771 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3350 3350 Size: 6961 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2408 2957 3164 3490 3499 3778 Size: 399 355 263 549 207 326 9 279 Cuts at: 3778 3830 4229 4280 5022 5107 6178 1391 Size: 52 399 51 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 549 399 399 355 326 279 263 207 85 52 51 9 BspEI T'CCGG_A Cuts at: 6624 6624 Size: 6961 BspHI T'CATG_A Cuts at: 2182 4850 6580 2182 Size: 2668 1730 2563 Fragments arranged by size: 2668 2563 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2050 3237 3723 4185 4316 844 Size: 1206 1187 486 462 131 3489 Fragments arranged by size: 3489 1206 1187 486 462 131 BsrBI CCG'CTC Cuts at: 1394 1823 3408 3865 4848 1394 Size: 429 1585 457 983 3507 Fragments arranged by size: 3507 1585 983 457 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5456 5630 65 Size: 1183 4208 174 1396 Fragments arranged by size: 4208 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5605 5792 1700 Size: 110 3795 187 2869 Fragments arranged by size: 3795 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6961 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6961 BssSI C'ACGA_G Cuts at: 2555 5021 6033 2555 Size: 2466 1012 3483 Fragments arranged by size: 3483 2466 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6961 BstBI TT'CG_AA Cuts at: 5765 5765 Size: 6961 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6961 BstXI CCAn_nnnn'nTGG Cuts at: 2569 4040 2569 Size: 1471 5490 BstYI r'GATC_y Cuts at: 1972 4291 4658 5043 5060 6512 6598 6610 6956 Size: 2319 367 385 17 1452 86 12 346 Cuts at: 6956 1972 Size: 1977 Fragments arranged by size: 2319 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4194 4194 Size: 6961 BtgI C'CryG_G Cuts at: 513 1065 2091 3623 513 Size: 552 1026 1532 3851 Fragments arranged by size: 3851 1532 1026 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6222 Fragments arranged by size: 6222 683 56 BtsI GCAGTG_nn' Cuts at: 4477 5271 5291 4477 Size: 794 20 6147 Fragments arranged by size: 6147 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6961 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6926 DraI TTT'AAA Cuts at: 4265 4265 Size: 6961 DraIII CAC_nnn'GTG Cuts at: 3485 3485 Size: 6961 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2444 5968 809 Size: 507 1128 3524 1802 Fragments arranged by size: 3524 1802 1128 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4408 5297 6926 Size: 54 1425 50 275 18 2578 889 1629 Cuts at: 6926 8 Size: 43 Fragments arranged by size: 2578 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4408 1537 Size: 275 2596 4090 Fragments arranged by size: 4090 2596 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3288 4891 1353 Size: 1935 1603 3423 Fragments arranged by size: 3423 1935 1603 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3461 5548 5922 6068 387 Size: 1064 138 1872 2087 374 146 1280 Fragments arranged by size: 2087 1872 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2955 3828 2955 Size: 873 6088 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2015 2075 2264 2357 2911 3312 4022 Size: 393 383 60 189 93 554 401 710 Cuts at: 4022 4039 4145 4161 5024 5621 6408 6676 1239 Size: 17 106 16 863 597 787 268 1524 Fragments arranged by size: 1524 863 787 710 597 554 401 393 383 268 189 106 93 60 17 16 EcoNI CCTnn'n_nnAGG Cuts at: 2057 3782 2057 Size: 1725 5236 EcoO109I rG'GnC_Cy Cuts at: 3275 3801 3275 Size: 526 6435 EcoRI G'AATT_C Cuts at: 4235 4270 4648 4235 Size: 35 378 6548 Fragments arranged by size: 6548 378 35 FspI TGC'GCA Cuts at: 5467 5467 Size: 6961 HaeII r_GCGC'y Cuts at: 1678 2748 6108 1678 Size: 1070 3360 2531 Fragments arranged by size: 3360 2531 1070 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2177 2209 2362 2394 3349 Size: 32 135 32 605 32 153 32 955 Cuts at: 3349 3381 4196 4228 5602 5634 5676 5708 1373 Size: 32 815 32 1374 32 42 32 2626 Fragments arranged by size: 2626 1374 955 815 605 153 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4305 4551 669 Size: 988 281 2367 246 3079 Fragments arranged by size: 3079 2367 988 281 246 HindIII A'AGCT_T Cuts at: 748 3584 748 Size: 2836 4125 HpaI GTT'AAC Cuts at: 4551 4551 Size: 6961 KpnI G_GTAC'C Cuts at: 4286 4286 Size: 6961 MfeI C'AATT_G Cuts at: 3233 3323 4560 3233 Size: 90 1237 5634 Fragments arranged by size: 5634 1237 90 MluI A'CGCG_T Cuts at: 6837 6837 Size: 6961 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2829 3411 6075 6259 1305 Size: 1524 582 2664 184 2007 Fragments arranged by size: 2664 2007 1524 582 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5139 Fragments arranged by size: 5139 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2411 3173 3440 3853 3857 4919 Size: 648 843 402 762 267 413 4 1062 Cuts at: 4919 5278 5437 518 Size: 359 159 2042 Fragments arranged by size: 2042 1062 843 762 648 413 402 359 267 159 4 MspA1I CmG'CkG Cuts at: 1606 2200 2331 2599 2785 3007 3565 4377 5054 Size: 594 131 268 186 222 558 812 677 Cuts at: 5054 6202 6447 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 812 677 594 558 268 245 222 186 131 NcoI C'CATG_G Cuts at: 513 1065 2091 513 Size: 552 1026 5383 Fragments arranged by size: 5383 1026 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6961 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6961 NotI GC'GGCC_GC Cuts at: 4408 4408 Size: 6961 NspI r_CATG'y Cuts at: 1132 1229 2986 3439 3574 4319 5864 1132 Size: 97 1757 453 135 745 1545 2229 Fragments arranged by size: 2229 1757 1545 745 453 135 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6961 PciI A'CATG_T Cuts at: 3570 5860 3570 Size: 2290 4671 PflMI CCAn_nnn'nTGG Cuts at: 1108 3426 1108 Size: 2318 4643 PfoI T'CCnGG_A Cuts at: 2052 2052 Size: 6961 PmeI GTTT'AAAC Cuts at: 4265 4265 Size: 6961 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5030 5062 6543 6575 5030 Size: 32 1481 32 5416 Fragments arranged by size: 5416 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3275 3801 3275 Size: 526 6435 PshAI GACnn'nnGTC Cuts at: 1613 3023 1613 Size: 1410 5551 PsiI TTA'TAA Cuts at: 3078 4531 3078 Size: 1453 5508 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2586 2618 2586 Size: 32 6929 PstI C_TGCA'G Cuts at: 830 2790 2823 4313 830 Size: 1960 33 1490 3478 Fragments arranged by size: 3478 1960 1490 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6961 PvuII CAG'CTG Cuts at: 2200 2331 2599 2785 3565 2200 Size: 131 268 186 780 5596 Fragments arranged by size: 5596 780 268 186 131 SacI G_AGCT'C Cuts at: 2957 3830 2957 Size: 873 6088 SalI G'TCGA_C Cuts at: 1936 4303 1936 Size: 2367 4594 SapI GCTCTTCn'nnn_ Cuts at: 3288 3288 Size: 6961 SbfI CC_TGCA'GG Cuts at: 4313 4313 Size: 6961 ScaI AGT'ACT Cuts at: 5209 6921 5209 Size: 1712 5249 SfcI C'TryA_G Cuts at: 826 1045 2246 2786 2802 2819 4230 4309 5444 Size: 219 1201 540 16 17 1411 79 1135 Cuts at: 5444 6125 6316 826 Size: 681 191 1471 Fragments arranged by size: 1471 1411 1201 1135 681 540 219 191 79 17 16 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6961 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6961 SmaI CCC'GGG Cuts at: 1872 3833 4288 1872 Size: 1961 455 4545 Fragments arranged by size: 4545 1961 455 SmlI C'TyrA_G Cuts at: 820 1017 1943 2606 3353 5065 5966 6228 820 Size: 197 926 663 747 1712 901 262 1553 Fragments arranged by size: 1712 1553 926 901 747 663 262 197 SnaBI TAC'GTA Cuts at: 493 4752 493 Size: 4259 2702 SpeI A'CTAG_T Cuts at: 152 2716 152 Size: 2564 4397 SphI G_CATG'C Cuts at: 1132 2986 4319 1132 Size: 1854 1333 3774 Fragments arranged by size: 3774 1854 1333 SspI AAT'ATT Cuts at: 5 52 2398 3985 4021 4885 5 Size: 47 2346 1587 36 864 2081 Fragments arranged by size: 2346 2081 1587 864 47 36 StuI AGG'CCT Cuts at: 1517 1517 Size: 6961 StyI C'CwwG_G Cuts at: 513 1065 2091 4400 513 Size: 552 1026 2309 3074 Fragments arranged by size: 3074 2309 1026 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3726 3847 4988 5005 5158 5343 5788 Size: 1255 2146 121 1141 17 153 185 445 Cuts at: 5788 325 Size: 1498 Fragments arranged by size: 2146 1498 1255 1141 445 185 153 121 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5207 6919 96 Size: 274 80 33 51 1443 3230 1712 138 Fragments arranged by size: 3230 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6921 6953 6921 Size: 32 6929 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6961 XbaI T'CTAG_A Cuts at: 4154 4297 4154 Size: 143 6818 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2773 3205 3489 2773 Size: 432 284 6245 Fragments arranged by size: 6245 432 284 XhoI C'TCGA_G Cuts at: 1943 3353 1943 Size: 1410 5551 XmaI C'CCGG_G Cuts at: 1870 3831 4286 1870 Size: 1961 455 4545 Fragments arranged by size: 4545 1961 455 XmnI GAAnn'nnTTC Cuts at: 2237 2649 2795 3297 5090 2237 Size: 412 146 502 1793 4108 Fragments arranged by size: 4108 1793 502 412 146 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4769 276 Size: 53 83 186 936 3235 2468 Fragments arranged by size: 3235 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI