Restriction map for pFN21AB3682
 (Linear) (Six-base) MAPSORT of: KIBB3682.seq  Check: 3431  from: 1  to: 2256



KIBB3682.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1232    1718    2256 
   Size:       1232     486     538

  Fragments arranged by size: 

                1232     538     486
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      10     259     352     906    1307    2017    2140    2156 
   Size:         10     249      93     554     401     710     123      16

Cuts at:   2156    2256 
   Size:        100

  Fragments arranged by size: 

                 710     554     401     249     123     100      93      16
                  10
 
AflIII A'CryG_T

Cuts at:      0    1565    2256 
   Size:       1565     691
 
AhdI GACnn_n'nnGTC

Cuts at:      0     186    2205    2256 
   Size:        186    2019      51

  Fragments arranged by size: 

                2019     186      51
 
AleI CACnn'nnGTG

Cuts at:      0    1168    1852    2256 
   Size:       1168     684     404

  Fragments arranged by size: 

                1168     684     404
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     550    2256 
   Size:        550    1706
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1871    1903    2256 
   Size:       1871      32     353

  Fragments arranged by size: 

                1871     353      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     105    2256 
   Size:        105    2151
 
ApaLI G'TGCA_C

Cuts at:      0    1481    2256 
   Size:       1481     775
 
ApoI r'AATT_y

Cuts at:      0      43     347    1213    1656    2230    2256 
   Size:         43     304     866     443     574      26

  Fragments arranged by size: 

                 866     574     443     304      43      26
 
AseI AT'TA_AT

Cuts at:      0     701    2256 
   Size:        701    1555
 
AvaI C'yCGr_G

Cuts at:      0    1348    1398    1826    2256 
   Size:       1348      50     428     430

  Fragments arranged by size: 

                1348     430     428      50
 
BanI G'GyrC_C

Cuts at:      0    1220    1252    2256 
   Size:       1220      32    1004

  Fragments arranged by size: 

                1220    1004      32
 
BanII G_rGCy'C

Cuts at:      0     952    1494    1825    2224    2256 
   Size:        952     542     331     399      32

  Fragments arranged by size: 

                 952     542     399     331      32
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     299    1018    2256 
   Size:        299     719    1238

  Fragments arranged by size: 

                1238     719     299
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     805     839    1344    1378    2256 
   Size:        805      34     505      34     878

  Fragments arranged by size: 

                 878     805     505      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2018    2256 
   Size:       2018     238
 
BclI T'GATC_A

Cuts at:      0     201     314     576    2256 
   Size:        201     113     262    1680

  Fragments arranged by size: 

                1680     262     201     113
 
BlpI GC'TnA_GC

Cuts at:      0    1493    2256 
   Size:       1493     763
 
Bme1580I G_kGCm'C

Cuts at:      0    1159    1485    1773    2256 
   Size:       1159     326     288     483

  Fragments arranged by size: 

                1159     483     326     288
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     141    2256 
   Size:        141    2115
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1314    1374    2256 
   Size:       1314      60     882

  Fragments arranged by size: 

                1314     882      60
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70    2034    2256 
   Size:         70    1964     222

  Fragments arranged by size: 

                1964     222      70
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     586    2256 
   Size:        586    1670
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     547    1549    1615    2256 
   Size:        547    1002      66     641

  Fragments arranged by size: 

                1002     641     547      66
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1697    1727    2256 
   Size:       1697      30     529

  Fragments arranged by size: 

                1697     529      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1198    2206    2256 
   Size:       1198    1008      50

  Fragments arranged by size: 

                1198    1008      50
 
BsiHKAI G_wGCw'C

Cuts at:      0     140     403     952    1485    1825    2256 
   Size:        140     263     549     533     340     431

  Fragments arranged by size: 

                 549     533     431     340     263     140
 
BsmI GAATG_Cn'

Cuts at:      0     696    2256 
   Size:        696    1560
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1345    2256 
   Size:       1345     911
 
Bsp1286I G_dGCh'C

Cuts at:      0     140     403     952    1159    1485    1494    1773    1825 
   Size:        140     263     549     207     326       9     279      52

Cuts at:   1825    2224    2256 
   Size:        399      32

  Fragments arranged by size: 

                 549     399     326     279     263     207     140      52
                  32       9
 
BspHI T'CATG_A

Cuts at:      0     177    2256 
   Size:        177    2079
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      45    1232    1718    2180    2256 
   Size:         45    1187     486     462      76

  Fragments arranged by size: 

                1187     486     462      76      45
 
BsrBI CCG'CTC

Cuts at:      0    1403    1860    2256 
   Size:       1403     457     396

  Fragments arranged by size: 

                1403     457     396
 
BssSI C'ACGA_G

Cuts at:      0     550    2256 
   Size:        550    1706
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     564    2035    2256 
   Size:        564    1471     221

  Fragments arranged by size: 

                1471     564     221
 
Bsu36I CC'TnA_GG

Cuts at:      0    2189    2256 
   Size:       2189      67
 
BtgI C'CryG_G

Cuts at:      0      86    1618    2256 
   Size:         86    1532     638

  Fragments arranged by size: 

                1532     638      86
 
DraIII CAC_nnn'GTG

Cuts at:      0    1480    2256 
   Size:       1480     776
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     439    2256 
   Size:        439    1817
 
EarI CTCTTCn'nnn_

Cuts at:      0    1283    2256 
   Size:       1283     973
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1456    2256 
   Size:       1456     800
 
EcoICRI GAG'CTC

Cuts at:      0     950    1823    2256 
   Size:        950     873     433

  Fragments arranged by size: 

                 950     873     433
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      10      70     259     352     906    1307    2017    2034 
   Size:         10      60     189      93     554     401     710      17

Cuts at:   2034    2140    2156    2256 
   Size:        106      16     100

  Fragments arranged by size: 

                 710     554     401     189     106     100      93      60
                  17      16      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      52    1777    2256 
   Size:         52    1725     479

  Fragments arranged by size: 

                1725     479      52
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1270    1796    2256 
   Size:       1270     526     460

  Fragments arranged by size: 

                1270     526     460
 
EcoRI G'AATT_C

Cuts at:      0    2230    2256 
   Size:       2230      26
 
HaeII r_GCGC'y

Cuts at:      0     743    2256 
   Size:        743    1513
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     172     204     357     389    1344    1376    2191    2223 
   Size:        172      32     153      32     955      32     815      32

Cuts at:   2223    2256 
   Size:         33

  Fragments arranged by size: 

                 955     815     172     153      33      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0    1579    2256 
   Size:       1579     677
 
MfeI C'AATT_G

Cuts at:      0    1228    1318    2256 
   Size:       1228      90     938

  Fragments arranged by size: 

                1228     938      90
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     824    1406    2256 
   Size:        824     582     850

  Fragments arranged by size: 

                 850     824     582
 
MslI CAynn'nnrTG

Cuts at:      0     406    1168    1435    1848    1852    2256 
   Size:        406     762     267     413       4     404

  Fragments arranged by size: 

                 762     413     406     404     267       4
 
MspA1I CmG'CkG

Cuts at:      0     195     326     594     780    1002    1560    2256 
   Size:        195     131     268     186     222     558     696

  Fragments arranged by size: 

                 696     558     268     222     195     186     131
 
NcoI C'CATG_G

Cuts at:      0      86    2256 
   Size:         86    2170
 
NspI r_CATG'y

Cuts at:      0     981    1434    1569    2256 
   Size:        981     453     135     687

  Fragments arranged by size: 

                 981     687     453     135
 
PciI A'CATG_T

Cuts at:      0    1565    2256 
   Size:       1565     691
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1421    2256 
   Size:       1421     835
 
PfoI T'CCnGG_A

Cuts at:      0      47    2256 
   Size:         47    2209
 
PpuMI rG'GwC_Cy

Cuts at:      0    1270    1796    2256 
   Size:       1270     526     460

  Fragments arranged by size: 

                1270     526     460
 
PshAI GACnn'nnGTC

Cuts at:      0    1018    2256 
   Size:       1018    1238
 
PsiI TTA'TAA

Cuts at:      0    1073    2256 
   Size:       1073    1183
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     581     613    2256 
   Size:        581      32    1643

  Fragments arranged by size: 

                1643     581      32
 
PstI C_TGCA'G

Cuts at:      0     785     818    2256 
   Size:        785      33    1438

  Fragments arranged by size: 

                1438     785      33
 
PvuII CAG'CTG

Cuts at:      0     195     326     594     780    1560    2256 
   Size:        195     131     268     186     780     696

  Fragments arranged by size: 

                 780     696     268     195     186     131
 
SacI G_AGCT'C

Cuts at:      0     952    1825    2256 
   Size:        952     873     431

  Fragments arranged by size: 

                 952     873     431
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1283    2256 
   Size:       1283     973
 
SfcI C'TryA_G

Cuts at:      0     241     781     797     814    2225    2256 
   Size:        241     540      16      17    1411      31

  Fragments arranged by size: 

                1411     540     241      31      17      16
 
SmaI CCC'GGG

Cuts at:      0    1828    2256 
   Size:       1828     428
 
SmlI C'TyrA_G

Cuts at:      0     601    1348    2256 
   Size:        601     747     908

  Fragments arranged by size: 

                 908     747     601
 
SpeI A'CTAG_T

Cuts at:      0     711    2256 
   Size:        711    1545
 
SphI G_CATG'C

Cuts at:      0     981    2256 
   Size:        981    1275
 
SspI AAT'ATT

Cuts at:      0     393    1980    2016    2256 
   Size:        393    1587      36     240

  Fragments arranged by size: 

                1587     393     240      36
 
StyI C'CwwG_G

Cuts at:      0      86    2256 
   Size:         86    2170
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1721    1842    2256 
   Size:       1721     121     414

  Fragments arranged by size: 

                1721     414     121
 
XbaI T'CTAG_A

Cuts at:      0    2149    2256 
   Size:       2149     107
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     768    1200    1484    2256 
   Size:        768     432     284     772

  Fragments arranged by size: 

                 772     768     432     284
 
XhoI C'TCGA_G

Cuts at:      0    1348    2256 
   Size:       1348     908
 
XmaI C'CCGG_G

Cuts at:      0    1826    2256 
   Size:       1826     430
 
XmnI GAAnn'nnTTC

Cuts at:      0     232     644     790    1292    2256 
   Size:        232     412     146     502     964

  Fragments arranged by size: 

                 964     502     412     232     146
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AhdI     AleI     AlfI     AloI    AlwNI
    ApaLI     ApoI     AseI     AvaI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI
     BsaI    BsaXI    BseRI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI
    BspMI    BsrBI    BssSI    BstXI   Bsu36I     BtgI   DraIII     DrdI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII
    Hin4I  HindIII     MfeI     MmeI     MslI   MspA1I     NcoI     NspI
     PciI    PflMI     PfoI    PpuMI    PshAI     PsiI     PsrI     PstI
    PvuII     SacI     SapI     SfcI     SmaI     SmlI     SpeI     SphI
     SspI     StyI    TaqII     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     ApaI
     AscI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI    BfrBI
     BglI    BglII    BmgBI     BmtI   Bpu10I    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI     BsgI    BsiEI    BsiWI    BspEI    BsrDI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstYI  BstZ17I    BtgZI
     BtsI     ClaI    CspCI     DraI     EaeI     EagI    EcoRV     FalI
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MluI
     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PmeI     PmlI     PpiI   PspOMI    PspXI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SrfI     StuI     SwaI
     TatI     TstI  Tth111I     ZraI
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