(Circular) (Six-base) MAPSORT of: pFN21AB3681.seq Check: 1800 from: 1 to: 7390 pFN21AB3681 7390 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5200 278 Size: 53 83 186 936 3664 2468 Fragments arranged by size: 3664 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4733 1937 Size: 2796 4594 Acc65I G'GTAC_C Cuts at: 4711 4711 Size: 7390 AclI AA'CG_TT Cuts at: 1563 5517 5890 1563 Size: 3954 373 3063 Fragments arranged by size: 3954 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2477 3542 3893 4492 5453 6837 7105 1632 Size: 845 1065 351 599 961 1384 268 1917 Fragments arranged by size: 1917 1384 1065 961 845 599 351 268 AfeI AGC'GCT Cuts at: 3800 3800 Size: 7390 AflII C'TTAA_G Cuts at: 820 1017 3599 820 Size: 197 2582 4611 Fragments arranged by size: 4611 2582 197 AflIII A'CryG_T Cuts at: 1203 4575 6289 7266 1203 Size: 3372 1714 977 1327 Fragments arranged by size: 3372 1714 1327 977 AgeI A'CCGG_T Cuts at: 4627 6221 4627 Size: 1594 5796 AhdI GACnn_n'nnGTC Cuts at: 6119 6119 Size: 7390 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7390 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3498 4304 1184 Size: 2314 806 4270 Fragments arranged by size: 4270 2314 806 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3182 3214 3220 3221 3252 3253 3182 Size: 32 6 1 31 1 7319 Fragments arranged by size: 7319 32 31 6 1 1 AlwNI CAG_nnn'CTG Cuts at: 2699 2934 3889 4259 5133 6705 2699 Size: 235 955 370 874 1572 3384 Fragments arranged by size: 3384 1572 955 874 370 235 ApaI G_GGCC'C Cuts at: 2269 2474 2820 2919 2946 4114 4593 2269 Size: 205 346 99 27 1168 479 5066 Fragments arranged by size: 5066 1168 479 346 205 99 27 ApaLI G'TGCA_C Cuts at: 4150 5447 6603 4150 Size: 1297 1156 4937 Fragments arranged by size: 4937 1297 1156 ApoI r'AATT_y Cuts at: 1493 4699 4925 5077 7186 1493 Size: 3206 226 152 2109 1697 Fragments arranged by size: 3206 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5944 160 Size: 872 4912 1606 Fragments arranged by size: 4912 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7390 AvaI C'yCGr_G Cuts at: 1870 1943 2023 2580 2774 2820 3806 4439 4585 Size: 73 80 557 194 46 986 633 146 Cuts at: 4585 4677 4715 1870 Size: 92 38 4545 Fragments arranged by size: 4545 986 633 557 194 146 92 80 73 46 38 BamHI G'GATC_C Cuts at: 2173 2331 4720 5087 2173 Size: 158 2389 367 4476 Fragments arranged by size: 4476 2389 367 158 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2030 2075 2464 2485 2836 Size: 325 217 627 243 45 389 21 351 Cuts at: 2836 3976 4056 4180 4624 4711 6166 618 Size: 1140 80 124 444 87 1455 1842 Fragments arranged by size: 1842 1455 1140 627 444 389 351 325 243 217 124 87 80 45 21 BanII G_rGCy'C Cuts at: 1391 2023 2049 2147 2269 2297 2474 2541 2820 Size: 632 26 98 122 28 177 67 279 Cuts at: 2820 2919 2946 3147 3579 3755 3813 4032 4114 Size: 99 27 201 432 176 58 219 82 Cuts at: 4114 4381 4593 4709 1391 Size: 267 212 116 4072 Fragments arranged by size: 4072 632 432 279 267 219 212 201 177 176 122 116 99 98 82 67 58 28 27 26 BbeI G_GCGC'C Cuts at: 2034 2079 2034 Size: 45 7345 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3939 4465 928 Size: 971 2040 526 3853 Fragments arranged by size: 3853 2040 971 526 BbvCI CC'TCA_GC Cuts at: 4496 4496 Size: 7390 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3459 3493 5581 5615 1903 Size: 34 50 34 1438 34 2088 34 3678 Fragments arranged by size: 3678 2088 1438 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2647 5282 6492 2647 Size: 2635 1210 3545 Fragments arranged by size: 3545 2635 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7390 BfrBI ATG'CAT Cuts at: 2008 3511 2008 Size: 1503 5887 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4256 4563 6001 Size: 107 122 71 631 605 2584 307 1438 Cuts at: 6001 136 Size: 1525 Fragments arranged by size: 2584 1525 1438 631 605 307 122 107 71 BglII A'GATC_T Cuts at: 7385 7385 Size: 7390 BlpI GC'TnA_GC Cuts at: 4807 4807 Size: 7390 Bme1580I G_kGCm'C Cuts at: 1790 2269 2467 2474 2490 2766 2820 2841 2919 Size: 479 198 7 16 276 54 21 78 Cuts at: 2919 2946 3078 3208 3678 3718 3979 4114 4154 Size: 27 132 130 470 40 261 135 40 Cuts at: 4154 4593 5451 6607 1790 Size: 439 858 1156 2573 Fragments arranged by size: 2573 1156 858 479 470 439 276 261 198 135 132 130 78 54 40 40 27 21 16 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7390 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2173 2241 2616 Size: 416 53 83 399 24 756 68 375 Cuts at: 2616 3065 3853 5121 6079 442 Size: 449 788 1268 958 1753 Fragments arranged by size: 1753 1268 958 788 756 449 416 399 375 83 68 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7390 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2130 2202 2309 2555 3041 3368 3470 3797 Size: 891 72 107 246 486 327 102 327 Cuts at: 3797 3911 4259 6050 1239 Size: 114 348 1791 2579 Fragments arranged by size: 2579 1791 891 486 348 327 327 246 114 107 102 72 Bpu10I CC'TnA_GC Cuts at: 2146 4496 2146 Size: 2350 5040 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3371 3612 5515 6380 6678 3371 Size: 241 1903 865 298 4083 Fragments arranged by size: 4083 1903 865 298 241 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2885 6053 882 Size: 617 1386 3168 2219 Fragments arranged by size: 3168 2219 1386 617 BsaAI yAC'GTr Cuts at: 493 1204 5181 493 Size: 711 3977 2702 Fragments arranged by size: 3977 2702 711 BsaBI GATnn'nnATC Cuts at: 7384 7384 Size: 7390 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2031 2076 5197 5579 Size: 53 83 186 936 498 45 3121 382 Cuts at: 5579 6214 275 Size: 635 1451 Fragments arranged by size: 3121 1451 936 635 498 382 186 83 53 45 BsaWI w'CCGG_w Cuts at: 2328 4627 5823 6221 6495 6642 7053 2328 Size: 2299 1196 398 274 147 411 2665 Fragments arranged by size: 2665 2299 1196 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3266 3296 4121 4151 1178 Size: 30 165 30 1863 30 825 30 4417 Fragments arranged by size: 4417 1863 825 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2903 3038 3041 3053 3056 3059 3563 3842 Size: 1717 135 3 12 3 3 504 279 Cuts at: 3842 3845 4634 1186 Size: 3 789 3942 Fragments arranged by size: 3942 1717 789 504 279 135 12 3 3 3 3 BseYI C'CCAG_C Cuts at: 2394 2498 2705 2766 2921 4218 4398 6593 7089 Size: 104 207 61 155 1297 180 2195 496 Cuts at: 7089 2394 Size: 2695 Fragments arranged by size: 2695 2195 1297 496 207 180 155 104 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2300 3134 3320 3377 4274 1163 Size: 573 564 834 186 57 897 4279 Fragments arranged by size: 4279 897 834 573 564 186 57 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3249 3658 4840 5601 6629 1540 Size: 275 186 1248 409 1182 761 1028 2301 Fragments arranged by size: 2301 1248 1182 1028 761 409 275 186 BsiHKAI G_wGCw'C Cuts at: 3147 3477 3579 3755 3813 4100 4154 5451 5536 Size: 330 102 176 58 287 54 1297 85 Cuts at: 5536 6607 3147 Size: 1071 3930 Fragments arranged by size: 3930 1297 1071 330 287 176 102 85 58 54 BsmI GAATG_Cn' Cuts at: 3487 3513 4901 4994 3487 Size: 26 1388 93 5883 Fragments arranged by size: 5883 1388 93 26 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2049 2147 2269 2297 2467 2474 Size: 399 233 26 98 122 28 170 7 Cuts at: 2474 2490 2541 2766 2820 2841 2919 2946 3078 Size: 16 51 225 54 21 78 27 132 Cuts at: 3078 3147 3208 3477 3579 3678 3718 3755 3813 Size: 69 61 269 102 99 40 37 58 Cuts at: 3813 3979 4032 4100 4114 4154 4381 4593 4709 Size: 166 53 68 14 40 227 212 116 Cuts at: 4709 5451 5536 6607 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 269 233 227 225 212 170 166 132 122 116 102 99 98 85 78 69 68 61 58 54 53 51 40 40 37 28 27 26 21 16 14 7 BspEI T'CCGG_A Cuts at: 7053 7053 Size: 7390 BspHI T'CATG_A Cuts at: 5279 7009 5279 Size: 1730 5660 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2514 2903 4745 844 Size: 1670 389 1842 3489 Fragments arranged by size: 3489 1842 1670 389 BsrBI CCG'CTC Cuts at: 1394 1823 2081 4137 4453 5277 1394 Size: 429 258 2056 316 824 3507 Fragments arranged by size: 3507 2056 824 429 316 258 BsrDI GCAATG_nn' Cuts at: 65 1248 3132 3329 5885 6059 65 Size: 1183 1884 197 2556 174 1396 Fragments arranged by size: 2556 1884 1396 1183 197 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2086 2988 4627 6034 6221 1700 Size: 110 276 902 1639 1407 187 2869 Fragments arranged by size: 2869 1639 1407 902 276 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7390 BssHII G'CGCG_C Cuts at: 1925 2135 1925 Size: 210 7180 BssSI C'ACGA_G Cuts at: 5450 6462 5450 Size: 1012 6378 BstAPI GCAn_nnn'nTGC Cuts at: 794 3343 3517 4020 4071 4158 4180 794 Size: 2549 174 503 51 87 22 4004 Fragments arranged by size: 4004 2549 503 174 87 51 22 BstBI TT'CG_AA Cuts at: 6194 6194 Size: 7390 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7390 BstXI CCAn_nnnn'nTGG Cuts at: 2596 2993 2596 Size: 397 6993 BstYI r'GATC_y Cuts at: 1972 2173 2331 3961 4720 5087 5472 5489 6941 Size: 201 158 1630 759 367 385 17 1452 Cuts at: 6941 7027 7039 7385 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1630 1452 759 385 367 346 201 158 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4383 4383 Size: 7390 BtgI C'CryG_G Cuts at: 513 1065 3259 3412 513 Size: 552 2194 153 4491 Fragments arranged by size: 4491 2194 552 153 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6651 Fragments arranged by size: 6651 683 56 BtsI GCAGTG_nn' Cuts at: 2564 4060 4647 4906 5700 5720 2564 Size: 1496 587 259 794 20 4234 Fragments arranged by size: 4234 1496 794 587 259 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7390 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7355 DraI TTT'AAA Cuts at: 4694 4694 Size: 7390 DraIII CAC_nnn'GTG Cuts at: 4011 4180 4011 Size: 169 7221 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6397 809 Size: 507 5081 1802 Fragments arranged by size: 5081 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2011 2058 2362 Size: 54 1425 50 275 18 181 47 304 Cuts at: 2362 2586 2976 3246 3694 4555 4837 5726 7355 Size: 224 390 270 448 861 282 889 1629 Cuts at: 7355 8 Size: 43 Fragments arranged by size: 1629 1425 889 861 448 390 304 282 275 270 224 181 54 50 47 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3246 4837 1537 Size: 275 1434 1591 4090 Fragments arranged by size: 4090 1591 1434 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3045 5320 1353 Size: 1692 2275 3423 Fragments arranged by size: 3423 2275 1692 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2073 5977 6351 6497 387 Size: 1064 138 484 3904 374 146 1280 Fragments arranged by size: 3904 1280 1064 484 374 146 138 EcoICRI GAG'CTC Cuts at: 3145 3577 3753 3811 3145 Size: 432 176 58 6724 Fragments arranged by size: 6724 432 176 58 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2130 2202 2309 2477 2555 3041 3368 Size: 393 498 72 107 168 78 486 327 Cuts at: 3368 3470 3542 3797 3893 3911 4259 4492 5453 Size: 102 72 255 96 18 348 233 961 Cuts at: 5453 6050 6837 7105 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 961 787 597 498 486 393 348 327 268 255 233 168 107 102 96 78 72 72 18 EcoNI CCTnn'n_nnAGG Cuts at: 3023 3191 3023 Size: 168 7222 EcoO109I rG'GnC_Cy Cuts at: 2266 2390 2417 2593 2714 2817 2916 2943 4589 Size: 124 27 176 121 103 99 27 1646 Cuts at: 4589 2266 Size: 5067 Fragments arranged by size: 5067 1646 176 124 121 103 99 27 27 EcoRI G'AATT_C Cuts at: 4699 5077 4699 Size: 378 7012 FspI TGC'GCA Cuts at: 5896 5896 Size: 7390 HaeII r_GCGC'y Cuts at: 1678 2034 2079 2214 3802 4225 6537 1678 Size: 356 45 135 1588 423 2312 2531 Fragments arranged by size: 2531 2312 1588 423 356 135 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 6031 6063 6105 6137 1373 Size: 32 135 32 4459 32 42 32 2626 Fragments arranged by size: 4459 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3010 3108 4734 4980 669 Size: 988 281 1072 98 1626 246 3079 Fragments arranged by size: 3079 1626 1072 988 281 246 98 HindIII A'AGCT_T Cuts at: 748 748 Size: 7390 HpaI GTT'AAC Cuts at: 4980 4980 Size: 7390 KasI G'GCGC_C Cuts at: 2030 2075 2030 Size: 45 7345 KpnI G_GTAC'C Cuts at: 4715 4715 Size: 7390 MfeI C'AATT_G Cuts at: 4989 4989 Size: 7390 MluI A'CGCG_T Cuts at: 7266 7266 Size: 7390 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2167 2904 3780 4164 6504 6688 1305 Size: 862 737 876 384 2340 184 2007 Fragments arranged by size: 2340 2007 876 862 737 384 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2013 2364 2588 3696 10 Size: 54 1425 343 181 351 224 1108 3704 Fragments arranged by size: 3704 1425 1108 351 343 224 181 54 MslI CAynn'nnrTG Cuts at: 518 1166 3480 4013 5348 5707 5866 518 Size: 648 2314 533 1335 359 159 2042 Fragments arranged by size: 2314 2042 1335 648 533 359 159 MspA1I CmG'CkG Cuts at: 1606 2398 2793 2853 4500 4806 5483 6631 6876 Size: 792 395 60 1647 306 677 1148 245 Cuts at: 6876 1606 Size: 2120 Fragments arranged by size: 2120 1647 1148 792 677 395 306 245 60 NaeI GCC'GGC Cuts at: 2088 2088 Size: 7390 NarI GG'CG_CC Cuts at: 2031 2076 2031 Size: 45 7345 NcoI C'CATG_G Cuts at: 513 1065 3412 513 Size: 552 2347 4491 Fragments arranged by size: 4491 2347 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7390 NgoMIV G'CCGG_C Cuts at: 2086 2086 Size: 7390 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7390 NotI GC'GGCC_GC Cuts at: 4837 4837 Size: 7390 NsiI A_TGCA'T Cuts at: 2010 3513 2010 Size: 1503 5887 NspI r_CATG'y Cuts at: 1132 1229 3346 4018 4082 4472 4748 6293 1132 Size: 97 2117 672 64 390 276 1545 2229 Fragments arranged by size: 2229 2117 1545 672 390 276 97 64 PasI CC'CwG_GG Cuts at: 1348 2604 2939 1348 Size: 1256 335 5799 Fragments arranged by size: 5799 1256 335 PciI A'CATG_T Cuts at: 6289 6289 Size: 7390 PflMI CCAn_nnn'nTGG Cuts at: 1108 2699 3187 3346 1108 Size: 1591 488 159 5152 Fragments arranged by size: 5152 1591 488 159 PfoI T'CCnGG_A Cuts at: 3186 3990 4236 3186 Size: 804 246 6340 Fragments arranged by size: 6340 804 246 PmeI GTTT'AAAC Cuts at: 4694 4694 Size: 7390 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5459 5491 6972 7004 5459 Size: 32 1481 32 5845 Fragments arranged by size: 5845 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2390 2417 2714 2390 Size: 27 297 7066 Fragments arranged by size: 7066 297 27 PshAI GACnn'nnGTC Cuts at: 1613 2415 3094 1613 Size: 802 679 5909 Fragments arranged by size: 5909 802 679 PsiI TTA'TAA Cuts at: 4960 4960 Size: 7390 PspOMI G'GGCC_C Cuts at: 2265 2470 2816 2915 2942 4110 4589 2265 Size: 205 346 99 27 1168 479 5066 Fragments arranged by size: 5066 1168 479 346 205 99 27 PspXI vC'TCGA_Gb Cuts at: 3806 3806 Size: 7390 PstI C_TGCA'G Cuts at: 830 2511 2632 4205 4264 4283 4742 830 Size: 1681 121 1573 59 19 459 3478 Fragments arranged by size: 3478 1681 1573 459 121 59 19 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7390 PvuII CAG'CTG Cuts at: 4500 4500 Size: 7390 SacI G_AGCT'C Cuts at: 3147 3579 3755 3813 3147 Size: 432 176 58 6724 Fragments arranged by size: 6724 432 176 58 SalI G'TCGA_C Cuts at: 1936 4732 1936 Size: 2796 4594 SbfI CC_TGCA'GG Cuts at: 4742 4742 Size: 7390 ScaI AGT'ACT Cuts at: 5638 7350 5638 Size: 1712 5678 SfcI C'TryA_G Cuts at: 826 1045 2507 2628 4201 4260 4279 4529 4738 Size: 219 1462 121 1573 59 19 250 209 Cuts at: 4738 5873 6554 6745 826 Size: 1135 681 191 1471 Fragments arranged by size: 1573 1471 1462 1135 681 250 219 209 191 121 59 19 SfiI GGCCn_nnn'nGGCC Cuts at: 4563 4563 Size: 7390 SfoI GGC'GCC Cuts at: 2032 2077 2032 Size: 45 7345 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7390 SgrAI Cr'CCGG_yG Cuts at: 1700 4627 1700 Size: 2927 4463 SmaI CCC'GGG Cuts at: 1872 2025 2822 4587 4717 1872 Size: 153 797 1765 130 4545 Fragments arranged by size: 4545 1765 797 153 130 SmlI C'TyrA_G Cuts at: 820 1017 1943 3386 3599 3627 3806 5494 6395 Size: 197 926 1443 213 28 179 1688 901 Cuts at: 6395 6657 820 Size: 262 1553 Fragments arranged by size: 1688 1553 1443 926 901 262 213 197 179 28 SnaBI TAC'GTA Cuts at: 493 5181 493 Size: 4688 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7390 SphI G_CATG'C Cuts at: 1132 4018 4748 1132 Size: 2886 730 3774 Fragments arranged by size: 3774 2886 730 SspI AAT'ATT Cuts at: 5 52 5314 5 Size: 47 5262 2081 Fragments arranged by size: 5262 2081 47 StuI AGG'CCT Cuts at: 1517 2786 3164 4070 4250 1517 Size: 1269 378 906 180 4657 Fragments arranged by size: 4657 1269 906 378 180 StyI C'CwwG_G Cuts at: 513 1065 2589 2973 3412 3691 4356 4829 513 Size: 552 1524 384 439 279 665 473 3074 Fragments arranged by size: 3074 1524 665 552 473 439 384 279 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3648 3958 4499 5417 5434 5587 5772 Size: 1255 2068 310 541 918 17 153 185 Cuts at: 5772 6217 325 Size: 445 1498 Fragments arranged by size: 2068 1498 1255 918 541 445 310 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5636 7348 96 Size: 274 80 33 51 1443 3659 1712 138 Fragments arranged by size: 3659 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7350 7382 7350 Size: 32 7358 Tth111I GACn'n_nGTC Cuts at: 1316 3254 3557 3845 1316 Size: 1938 303 288 4861 Fragments arranged by size: 4861 1938 303 288 XbaI T'CTAG_A Cuts at: 4726 4726 Size: 7390 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3496 3496 Size: 7390 XhoI C'TCGA_G Cuts at: 1943 3806 1943 Size: 1863 5527 XmaI C'CCGG_G Cuts at: 1870 2023 2820 4585 4715 1870 Size: 153 797 1765 130 4545 Fragments arranged by size: 4545 1765 797 153 130 XmnI GAAnn'nnTTC Cuts at: 5519 5519 Size: 7390 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5198 276 Size: 53 83 186 936 3664 2468 Fragments arranged by size: 3664 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NruI PacI PmlI PsrI RsrII SacII SanDI SapI SexAI SrfI SwaI