Restriction map for
pFN21AB3674
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspHI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmI BsoBI BspDI BspEI BspHI
BsrBI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI DsaI EagI
EclXI Eco52I Eco81I EcoNI EcoRI
EcoT14I EcoT22I FauI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I StuI
StyI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 1348 AAATTT [GA]^AATT[CT]
AflIII 1270 ACATGT A^C[GA][CT]GT
AhdI 931 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 456 AGCGCT AGC^GCT
ApoI 1348 AAATTT [GA]^AATT[CT]
AspEI 931 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 818 TGGCCA TGG^CCA
BanI 255 GGCACC G^G[CT][GA]CC
BanII 884 GGGCTC G[GA]GC[CT]^C
BclI 1019 TGATCA T^GATCA
BlpI 1504 GCTCAGC GC^T[ACGT]AGC
BpmI 887 CTCCAG CTGGAG(16/14)
Bpu1102I 1504 GCTCAGC GC^T[ACGT]AGC
BsaI 492 GGTCTC GGTCTC(1/5)
BsgI 91 CTGCAC GTGCAG(16/14)
BsmBI 654 CGTCTC CGTCTC(1/5)
BsmFI 998 GTCCC GGGAC(10/14)
BspLU11I 1270 ACATGT A^CATGT
BsrFI 252 ACCGGC [GA]^CCGG[CT]
BstAPI 193 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 40 GGTCACC G^GT[ACGT]ACC
BstPI 40 GGTCACC G^GT[ACGT]ACC
BstXI 729 CCATTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1504 GCTCAGC GC^T[ACGT]AGC
Cfr10I 252 ACCGGC [GA]^CCGG[CT]
DraII 1098 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 818 TGGCCA [CT]^GGCC[GA]
Eam1105I 931 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 456 AGCGCT AGC^GCT
EcoO109I 1098 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 40 GGTCACC G^GT[ACGT]ACC
EcoRV 1262 GATATC GAT^ATC
FbaI 1019 TGATCA T^GATCA
HaeIII 819 GGCC GG^CC
MaeII 476 ACGT A^CGT
MluNI 818 TGGCCA TGG^CCA
MscI 818 TGGCCA TGG^CCA
NdeI 850 CATATG CA^TATG
NspI 1270 ACATGT [GA]CATG^[CT]
PpuMI 1098 GGGTCCT [GA]G^G[AT]CC[CT]
SspI 978 AATATT AAT^ATT
XcmI 1001 CCATTCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Asp700I 1159 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1399 GAAATTATTC
AvaII 274 GGACC G^G[AT]CC
1099 GGTCC
BbsI 478 GTCTTC GAAGAC(2/6)
508 GTCTTC
BmyI 884 GGGCTC G[AGT]GC[ACT]^C
1035 GGGCAC
BpuAI 478 GTCTTC GAAGAC(2/6)
508 GTCTTC
Bsp1286I 884 GGGCTC G[AGT]GC[ACT]^C
1035 GGGCAC
Bsp1407I 144 TGTACA T^GTACA
153 TGTACA
BspMI 89 ACCTGC ACCTGC(4/8)
37 GCAGGT
BsrDI 920 GCAATG GCAATG(2/0)
1054 GCAATG
BsrGI 144 TGTACA T^GTACA
153 TGTACA
BstYI 432 AGATCC [GA]^GATC[CT]
1026 GGATCT
Cac8I 264 GCAGGC GC[ACGT]^[ACGT]GC
736 GCTGGC
Cfr13I 274 GGACC G^G[ACGT]CC
1099 GGTCC
HaeII 456 AGCGCT [GA]GCGC^[CT]
1075 GGCGCT
MflI 432 AGATCC [GA]^GATC[CT]
1026 GGATCT
PflMI 436 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
483 CCAGAATCTGG
PleI 855 GACTC GAGTC(4/5)
996 GAGTC
Sau96I 274 GGACC G^G[ACGT]CC
1099 GGTCC
SspBI 144 TGTACA T^GTACA
153 TGTACA
TaqI 750 TCGA T^CGA
1364 TCGA
TthHB8I 750 TCGA T^CGA
1364 TCGA
Van91I 436 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
483 CCAGAATCTGG
XhoII 432 AGATCC [GA]^GATC[CT]
1026 GGATCT
XmnI 1159 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1399 GAAATTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BbvI BcnI BfaI BsaJI BseRI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI CfoI DdeI DpnII
EarI EcoRII Fnu4HI FokI HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI PvuII RsaI
Sau3AI ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI