(Circular) (Six-base) MAPSORT of: pFN21AB3674.seq Check: 2904 from: 1 to: 6250 pFN21AB3674 6250 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2103 2103 Size: 6250 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4060 278 Size: 53 83 186 936 2524 2468 Fragments arranged by size: 2524 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3593 1937 Size: 1656 4594 Acc65I G'GTAC_C Cuts at: 3571 3571 Size: 6250 AclI AA'CG_TT Cuts at: 1563 4377 4750 1563 Size: 2814 373 3063 Fragments arranged by size: 3063 2814 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4313 5697 5965 1632 Size: 2681 1384 268 1917 Fragments arranged by size: 2681 1917 1384 268 AfeI AGC'GCT Cuts at: 2463 2463 Size: 6250 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6053 AflIII A'CryG_T Cuts at: 1203 3275 5149 6126 1203 Size: 2072 1874 977 1327 Fragments arranged by size: 2072 1874 1327 977 AgeI A'CCGG_T Cuts at: 5081 5081 Size: 6250 AhdI GACnn_n'nnGTC Cuts at: 2941 4979 2941 Size: 2038 4212 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6250 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6250 AlwNI CAG_nnn'CTG Cuts at: 2204 2374 2494 2793 3993 5565 2204 Size: 170 120 299 1200 1572 2889 Fragments arranged by size: 2889 1572 1200 299 170 120 ApaLI G'TGCA_C Cuts at: 4307 5463 4307 Size: 1156 5094 ApoI r'AATT_y Cuts at: 1493 3353 3559 3785 3937 6046 1493 Size: 1860 206 226 152 2109 1697 Fragments arranged by size: 2109 1860 1697 226 206 152 AseI AT'TA_AT Cuts at: 160 1032 4804 160 Size: 872 3772 1606 Fragments arranged by size: 3772 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6250 AvaI C'yCGr_G Cuts at: 1870 1943 3575 1870 Size: 73 1632 4545 Fragments arranged by size: 4545 1632 73 BamHI G'GATC_C Cuts at: 3580 3947 3580 Size: 367 5883 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2260 3571 5026 618 Size: 325 217 627 473 1311 1455 1842 Fragments arranged by size: 1842 1455 1311 627 473 325 217 BanII G_rGCy'C Cuts at: 1391 2893 3569 1391 Size: 1502 676 4072 Fragments arranged by size: 4072 1502 676 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2476 2506 928 Size: 971 577 30 4672 Fragments arranged by size: 4672 971 577 30 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4441 4475 1903 Size: 34 2504 34 3678 Fragments arranged by size: 3678 2504 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4142 5352 4142 Size: 1210 5040 BclI T'GATC_A Cuts at: 1548 3024 1548 Size: 1476 4774 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4861 136 Size: 107 122 71 631 605 337 2852 1525 Fragments arranged by size: 2852 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6245 6245 Size: 6250 BlpI GC'TnA_GC Cuts at: 3510 3667 3510 Size: 157 6093 Bme1580I G_kGCm'C Cuts at: 1790 3044 4311 5467 1790 Size: 1254 1267 1156 2573 Fragments arranged by size: 2573 1267 1254 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6250 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3981 4939 442 Size: 416 53 83 399 24 2564 958 1753 Fragments arranged by size: 2564 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6250 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2480 3470 2480 Size: 990 5260 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2877 4910 1239 Size: 1638 2033 2579 Fragments arranged by size: 2579 2033 1638 Bpu10I CC'TnA_GC Cuts at: 2301 3084 3222 3232 2301 Size: 783 138 10 5319 Fragments arranged by size: 5319 783 138 10 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4375 5240 5538 4375 Size: 865 298 5087 Fragments arranged by size: 5087 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2503 4913 882 Size: 617 1004 2410 2219 Fragments arranged by size: 2410 2219 1004 617 BsaAI yAC'GTr Cuts at: 493 1204 4041 493 Size: 711 2837 2702 Fragments arranged by size: 2837 2702 711 BsaBI GATnn'nnATC Cuts at: 6244 6244 Size: 6250 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4057 4439 5074 275 Size: 53 83 186 936 2524 382 635 1451 Fragments arranged by size: 2524 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4683 5081 5355 5502 5913 4683 Size: 398 274 147 411 5020 Fragments arranged by size: 5020 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6025 Fragments arranged by size: 6025 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2076 2079 2655 3525 1186 Size: 890 3 576 870 3911 Fragments arranged by size: 3911 890 870 576 3 BseYI C'CCAG_C Cuts at: 2296 5453 5949 2296 Size: 3157 496 2597 Fragments arranged by size: 3157 2597 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 1163 Size: 573 345 5332 Fragments arranged by size: 5332 573 345 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3700 4461 5489 1540 Size: 275 186 1699 761 1028 2301 Fragments arranged by size: 2301 1699 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4311 4396 5467 4311 Size: 85 1071 5094 Fragments arranged by size: 5094 1071 85 BsmI GAATG_Cn' Cuts at: 3761 3854 3761 Size: 93 6157 BsmBI CGTCTCn'nnnn_ Cuts at: 2665 2665 Size: 6250 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2893 3044 3569 4311 4396 5467 1391 Size: 399 1103 151 525 742 85 1071 2174 Fragments arranged by size: 2174 1103 1071 742 525 399 151 85 BspEI T'CCGG_A Cuts at: 5913 5913 Size: 6250 BspHI T'CATG_A Cuts at: 4139 5869 4139 Size: 1730 4520 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2033 2103 3605 844 Size: 1189 70 1502 3489 Fragments arranged by size: 3489 1502 1189 70 BsrBI CCG'CTC Cuts at: 1394 1823 4137 1394 Size: 429 2314 3507 Fragments arranged by size: 3507 2314 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2932 3066 4745 4919 65 Size: 1183 1684 134 1679 174 1396 Fragments arranged by size: 1684 1679 1396 1183 174 134 BsrFI r'CCGG_y Cuts at: 1700 1810 2257 4894 5081 1700 Size: 110 447 2637 187 2869 Fragments arranged by size: 2869 2637 447 187 110 BsrGI T'GTAC_A Cuts at: 96 2149 2158 96 Size: 2053 9 4188 Fragments arranged by size: 4188 2053 9 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6250 BssSI C'ACGA_G Cuts at: 4310 5322 4310 Size: 1012 5238 BstAPI GCAn_nnn'nTGC Cuts at: 794 2204 794 Size: 1410 4840 BstBI TT'CG_AA Cuts at: 5054 5054 Size: 6250 BstEII G'GTnAC_C Cuts at: 1184 2045 1184 Size: 861 5389 BstXI CCAn_nnnn'nTGG Cuts at: 2741 2741 Size: 6250 BstYI r'GATC_y Cuts at: 1972 2437 3031 3580 3947 4332 4349 5801 5887 Size: 465 594 549 367 385 17 1452 86 Cuts at: 5887 5899 6245 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 594 549 465 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4759 Fragments arranged by size: 4759 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5511 Fragments arranged by size: 5511 683 56 BtsI GCAGTG_nn' Cuts at: 3766 4560 4580 3766 Size: 794 20 5436 Fragments arranged by size: 5436 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6250 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6215 DraI TTT'AAA Cuts at: 3554 3554 Size: 6250 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5257 809 Size: 507 3941 1802 Fragments arranged by size: 3941 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2823 3697 4586 Size: 54 1425 50 275 18 993 874 889 Cuts at: 4586 6215 8 Size: 1629 43 Fragments arranged by size: 1629 1425 993 889 874 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3697 1537 Size: 275 1885 4090 Fragments arranged by size: 4090 1885 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2179 3129 3307 4180 1353 Size: 826 950 178 873 3423 Fragments arranged by size: 3423 950 873 826 178 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4837 5211 5357 387 Size: 1064 138 3248 374 146 1280 Fragments arranged by size: 3248 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2877 4313 4910 5697 5965 1239 Size: 393 1245 1436 597 787 268 1524 Fragments arranged by size: 1524 1436 1245 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3104 3104 Size: 6250 EcoRI G'AATT_C Cuts at: 3559 3937 3559 Size: 378 5872 EcoRV GAT'ATC Cuts at: 3269 3269 Size: 6250 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2717 3247 2717 Size: 530 5720 FspI TGC'GCA Cuts at: 4756 4756 Size: 6250 HaeII r_GCGC'y Cuts at: 1678 2465 3084 5397 1678 Size: 787 619 2313 2531 Fragments arranged by size: 2531 2313 787 619 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2927 2959 3016 3048 4891 Size: 32 135 32 1355 32 57 32 1843 Cuts at: 4891 4923 4965 4997 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1843 1355 135 57 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3594 3840 669 Size: 988 281 1656 246 3079 Fragments arranged by size: 3079 1656 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6250 HpaI GTT'AAC Cuts at: 3840 3840 Size: 6250 KpnI G_GTAC'C Cuts at: 3575 3575 Size: 6250 MfeI C'AATT_G Cuts at: 3849 3849 Size: 6250 MluI A'CGCG_T Cuts at: 6126 6126 Size: 6250 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2605 2997 3415 5364 5548 1305 Size: 1300 392 418 1949 184 2007 Fragments arranged by size: 2007 1949 1300 418 392 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2825 10 Size: 54 1425 343 993 3435 Fragments arranged by size: 3435 1425 993 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2553 3278 3280 4208 4567 4726 518 Size: 648 1387 725 2 928 359 159 2042 Fragments arranged by size: 2042 1387 928 725 648 359 159 2 MspA1I CmG'CkG Cuts at: 1606 2147 2183 2201 2210 2285 2321 2374 2741 Size: 541 36 18 9 75 36 53 367 Cuts at: 2741 3666 4343 5491 5736 1606 Size: 925 677 1148 245 2120 Fragments arranged by size: 2120 1148 925 677 541 367 245 75 53 36 36 18 9 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4759 Fragments arranged by size: 4759 939 552 NdeI CA'TA_TG Cuts at: 387 2856 387 Size: 2469 3781 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6250 NotI GC'GGCC_GC Cuts at: 3697 3697 Size: 6250 NspI r_CATG'y Cuts at: 1132 1229 3279 3608 5153 1132 Size: 97 2050 329 1545 2229 Fragments arranged by size: 2229 2050 1545 329 97 PasI CC'CwG_GG Cuts at: 1348 2228 1348 Size: 880 5370 PciI A'CATG_T Cuts at: 3275 5149 3275 Size: 1874 4376 PflMI CCAn_nnn'nTGG Cuts at: 1108 2447 2494 1108 Size: 1339 47 4864 Fragments arranged by size: 4864 1339 47 PfoI T'CCnGG_A Cuts at: 2275 2517 2275 Size: 242 6008 PmeI GTTT'AAAC Cuts at: 3554 3554 Size: 6250 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4319 4351 5832 5864 4319 Size: 32 1481 32 4705 Fragments arranged by size: 4705 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3104 3104 Size: 6250 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6250 PsiI TTA'TAA Cuts at: 3820 3820 Size: 6250 PstI C_TGCA'G Cuts at: 830 2035 2209 2215 2290 3602 830 Size: 1205 174 6 75 1312 3478 Fragments arranged by size: 3478 1312 1205 174 75 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6250 PvuII CAG'CTG Cuts at: 2183 2210 2285 2321 2374 2741 2183 Size: 27 75 36 53 367 5692 Fragments arranged by size: 5692 367 75 53 36 27 SalI G'TCGA_C Cuts at: 1936 3592 1936 Size: 1656 4594 SbfI CC_TGCA'GG Cuts at: 3602 3602 Size: 6250 ScaI AGT'ACT Cuts at: 4498 6210 4498 Size: 1712 4538 SfcI C'TryA_G Cuts at: 826 1045 2031 2205 2211 2286 2325 3598 4733 Size: 219 986 174 6 75 39 1273 1135 Cuts at: 4733 5414 5605 826 Size: 681 191 1471 Fragments arranged by size: 1471 1273 1135 986 681 219 191 174 75 39 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6250 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6250 SmaI CCC'GGG Cuts at: 1872 3577 1872 Size: 1705 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4354 5255 5517 820 Size: 197 926 2411 901 262 1553 Fragments arranged by size: 2411 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4041 493 Size: 3548 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6250 SphI G_CATG'C Cuts at: 1132 3608 1132 Size: 2476 3774 SspI AAT'ATT Cuts at: 5 52 2985 4174 5 Size: 47 2933 1189 2081 Fragments arranged by size: 2933 2081 1189 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6250 StyI C'CwwG_G Cuts at: 513 1065 2004 3689 513 Size: 552 939 1685 3074 Fragments arranged by size: 3074 1685 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4277 4294 4447 4632 5077 325 Size: 1255 2697 17 153 185 445 1498 Fragments arranged by size: 2697 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2149 2158 2557 Size: 274 80 33 51 1443 172 9 399 Cuts at: 2557 3130 4496 6208 96 Size: 573 1366 1712 138 Fragments arranged by size: 1712 1443 1366 573 399 274 172 138 80 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3058 3090 3432 3464 6210 6242 3058 Size: 32 342 32 2746 32 3066 Fragments arranged by size: 3066 2746 342 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6250 XbaI T'CTAG_A Cuts at: 3586 3586 Size: 6250 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3013 3013 Size: 6250 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6250 XmaI C'CCGG_G Cuts at: 1870 3575 1870 Size: 1705 4545 XmnI GAAnn'nnTTC Cuts at: 3168 3408 4379 3168 Size: 240 971 5039 Fragments arranged by size: 5039 971 240 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4058 276 Size: 53 83 186 936 2524 2468 Fragments arranged by size: 2524 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI