Restriction map for
pFN21AB3596
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII AccI AccIII AcyI AflII
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
AspEI AviII AvrII BalI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiWI BsmBI BsmI BspDI BspEI
BspLU11I BspMI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI Hin1I HindIII HpaI KasI
KspI MamI MluI MluNI MroI
MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
XcmI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 964 GGTACC G^GTACC
AccII 23 CGCG CG^CG
AclI 716 AACGTT AA^CGTT
AcsI 1256 GAATTC [GA]^AATT[CT]
Alw44I 257 GTGCAC G^TGCAC
ApaLI 257 GTGCAC G^TGCAC
ApoI 1256 GAATTC [GA]^AATT[CT]
Asp718I 964 GGTACC G^GTACC
AspHI 257 GTGCAC G[AT]GC[AT]^C
AspI 552 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 856 GGATCC G^GATCC
BanI 964 GGTACC G^G[CT][GA]CC
BanII 154 GGGCTC G[GA]GC[CT]^C
BsaI 178 GAGACC GGTCTC(1/5)
BsiEI 11 CGGTCG CG[GA][CT]^CG
BsiHKAI 257 GTGCAC G[AT]GC[AT]^C
BsmFI 26 GTCCC GGGAC(10/14)
Bsp1407I 121 TGTACA T^GTACA
BsrBI 930 GAGCGG CCGCTC(-3/-3)
BsrGI 121 TGTACA T^GTACA
BssSI 43 CTCGTG CACGAG(-5/-1)
BstF5I 774 CATCC GGATG(2/0)
BstUI 23 CGCG CG^CG
BstXI 1363 CCAAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 856 GGATCC [GA]^GATC[CT]
CfoI 76 GCGC GCG^C
DraII 485 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1111 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 923 CCACGG C^C[GA][CT]GG
EcoO109I 485 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1256 GAATTC G^AATTC
FauI 672 GCGGG CCCGC(4/6)
FokI 774 CATCC GGATG(9/13)
HaeII 75 GGCGCT [GA]GCGC^[CT]
HgaI 24 GCGTC GACGC(5/10)
HhaI 76 GCGC GCG^C
HinP1I 76 GCGC G^CGC
HincII 1487 GTCAAC GT[CT]^[GA]AC
HindII 1487 GTCAAC GT[CT]^[GA]AC
KpnI 964 GGTACC GGTAC^C
MfeI 599 CAATTG C^AATTG
MflI 856 GGATCC [GA]^GATC[CT]
MspA1I 380 CAGCTG C[AC]G^C[GT]G
MunI 599 CAATTG C^AATTG
MvnI 23 CGCG CG^CG
NspI 206 GCATGT [GA]CATG^[CT]
PaeR7I 236 CTCGAG C^TCGAG
PflFI 552 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1110 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 819 GACTC GAGTC(4/5)
PpuMI 485 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 716 AACGTT AA^CGTT
PvuII 380 CAGCTG CAG^CTG
SmaI 84 CCCGGG CCC^GGG
SspBI 121 TGTACA T^GTACA
Tth111I 552 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1110 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 706 TCTAGA T^CTAGA
XhoI 236 CTCGAG C^TCGAG
XhoII 856 GGATCC [GA]^GATC[CT]
XmaCI 84 CCCGGG C^CCGGG
XmaI 84 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 857 GATCC GGATC(4/5)
856 GGATC
AlwNI 560 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
887 CAGTTACTG
AvaI 84 CCCGGG C^[CT]CG[GA]G
236 CTCGAG
BfaI 707 CTAG C^TAG
1442 CTAG
BmyI 154 GGGCTC G[AGT]GC[ACT]^C
257 GTGCAC
BsmAI 178 GAGAC GTCTC(1/5)
166 GTCTC
BsoBI 84 CCCGGG C^[CT]CG[GA]G
236 CTCGAG
Bsp1286I 154 GGGCTC G[AGT]GC[ACT]^C
257 GTGCAC
BstNI 355 CCTGG CC^[AT]GG
777 CCTGG
EarI 264 CTCTTC CTCTTC(1/4)
481 GAAGAG
EcoRII 355 CCTGG ^CC[AT]GG
777 CCTGG
EcoT14I 360 CCTTGG C^C[AT][AT]GG
969 CCAAGG
HapII 30 CCGG C^CGG
85 CCGG
HpaII 30 CCGG C^CGG
85 CCGG
HphI 352 TCACC GGTGA(8/7)
462 TCACC
Ksp632I 264 CTCTTC CTCTTC(1/4)
481 GAAGAG
MaeI 707 CTAG C^TAG
1442 CTAG
MspI 30 CCGG C^CGG
85 CCGG
MvaI 355 CCTGG CC^[AT]GG
777 CCTGG
SfaNI 413 GCATC GCATC(5/9)
1499 GCATC
SmlI 236 CTCGAG C^T[CT][GA]AG
1389 CTTGAG
StyI 360 CCTTGG C^C[AT][AT]GG
969 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BcnI BsaJI BseRI BsiYI BslI
BspHI BsrDI BsrI BssKI Cac8I
Cfr13I DdeI DpnII Fnu4HI HaeIII
HinfI ItaI MaeII MaeIII MboI
MboII MnlI MseI MslI MwoI
NciI NdeII NlaIII NlaIV RcaI
RsaI Sau3AI Sau96I ScrFI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I