(Circular) (Six-base) MAPSORT of: pFN21AB3596.seq Check: 3150 from: 1 to: 6214 pFN21AB3596 6214 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4024 278 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3557 1937 Size: 1620 4594 Acc65I G'GTAC_C Cuts at: 2969 3535 2969 Size: 566 5648 AclI AA'CG_TT Cuts at: 1563 2722 4341 4714 1563 Size: 1159 1619 373 3063 Fragments arranged by size: 3063 1619 1159 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3168 4277 5661 5929 1632 Size: 1536 1109 1384 268 1917 Fragments arranged by size: 1917 1536 1384 1109 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6017 AflIII A'CryG_T Cuts at: 1203 5113 6090 1203 Size: 3910 977 1327 Fragments arranged by size: 3910 1327 977 AgeI A'CCGG_T Cuts at: 5045 5045 Size: 6214 AhdI GACnn_n'nnGTC Cuts at: 4943 4943 Size: 6214 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6214 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6214 AlwNI CAG_nnn'CTG Cuts at: 2570 2897 3957 5529 2570 Size: 327 1060 1572 3255 Fragments arranged by size: 3255 1572 1060 327 ApaLI G'TGCA_C Cuts at: 2262 4271 5427 2262 Size: 2009 1156 3049 Fragments arranged by size: 3049 2009 1156 ApoI r'AATT_y Cuts at: 1493 3261 3523 3749 3901 6010 1493 Size: 1768 262 226 152 2109 1697 Fragments arranged by size: 2109 1768 1697 262 226 152 AseI AT'TA_AT Cuts at: 160 1032 4768 160 Size: 872 3736 1606 Fragments arranged by size: 3736 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 AvaI C'yCGr_G Cuts at: 1870 1943 2089 2241 3539 1870 Size: 73 146 152 1298 4545 Fragments arranged by size: 4545 1298 152 146 73 BamHI G'GATC_C Cuts at: 2861 3544 3911 2861 Size: 683 367 5164 Fragments arranged by size: 5164 683 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2969 3535 4990 618 Size: 325 217 627 1182 566 1455 1842 Fragments arranged by size: 1842 1455 1182 627 566 325 217 BanII G_rGCy'C Cuts at: 1391 2163 3533 1391 Size: 772 1370 4072 Fragments arranged by size: 4072 1370 772 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5243 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4405 4439 1903 Size: 34 2468 34 3678 Fragments arranged by size: 3678 2468 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4106 5316 4106 Size: 1210 5004 BclI T'GATC_A Cuts at: 1548 1548 Size: 6214 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4825 136 Size: 107 122 71 631 605 337 2816 1525 Fragments arranged by size: 2816 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6209 6209 Size: 6214 BlpI GC'TnA_GC Cuts at: 3631 3631 Size: 6214 Bme1580I G_kGCm'C Cuts at: 1790 2266 4275 5431 1790 Size: 476 2009 1156 2573 Fragments arranged by size: 2573 2009 1156 476 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6214 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2289 3945 4903 Size: 416 53 83 399 24 872 1656 958 Cuts at: 4903 442 Size: 1753 Fragments arranged by size: 1753 1656 958 872 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6214 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4874 1239 Size: 3635 2579 Bpu10I CC'TnA_GC Cuts at: 2575 2575 Size: 6214 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3415 4339 5204 5502 3415 Size: 924 865 298 4127 Fragments arranged by size: 4127 924 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2177 4877 882 Size: 617 678 2700 2219 Fragments arranged by size: 2700 2219 678 617 BsaAI yAC'GTr Cuts at: 493 1204 4005 493 Size: 711 2801 2702 Fragments arranged by size: 2801 2702 711 BsaBI GATnn'nnATC Cuts at: 6208 6208 Size: 6214 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4021 4403 5038 275 Size: 53 83 186 936 2488 382 635 1451 Fragments arranged by size: 2488 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4647 5045 5319 5466 5877 4647 Size: 398 274 147 411 4984 Fragments arranged by size: 4984 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2673 2703 1178 Size: 30 165 30 1270 30 4689 Fragments arranged by size: 4689 1270 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2036 2698 1186 Size: 847 3 662 4702 Fragments arranged by size: 4702 847 662 3 BseYI C'CCAG_C Cuts at: 5417 5913 5417 Size: 496 5718 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5641 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2019 3664 4425 5453 1540 Size: 275 186 18 1645 761 1028 2301 Fragments arranged by size: 2301 1645 1028 761 275 186 18 BsiHKAI G_wGCw'C Cuts at: 2266 4275 4360 5431 2266 Size: 2009 85 1071 3049 Fragments arranged by size: 3049 2009 1071 85 BsmI GAATG_Cn' Cuts at: 3725 3818 3725 Size: 93 6121 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2163 2266 3533 4275 4360 5431 1391 Size: 399 373 103 1267 742 85 1071 2174 Fragments arranged by size: 2174 1267 1071 742 399 373 103 85 BspEI T'CCGG_A Cuts at: 5877 5877 Size: 6214 BspHI T'CATG_A Cuts at: 2770 2851 3265 4103 5833 2770 Size: 81 414 838 1730 3151 Fragments arranged by size: 3151 1730 838 414 81 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3569 844 Size: 2725 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2937 4101 1394 Size: 429 1114 1164 3507 Fragments arranged by size: 3507 1164 1114 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2317 2391 2585 2988 3219 3458 4709 Size: 1183 1069 74 194 403 231 239 1251 Cuts at: 4709 4883 65 Size: 174 1396 Fragments arranged by size: 1396 1251 1183 1069 403 239 231 194 174 74 BsrFI r'CCGG_y Cuts at: 1700 1810 4858 5045 1700 Size: 110 3048 187 2869 Fragments arranged by size: 3048 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2126 96 Size: 2030 4184 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6214 BssSI C'ACGA_G Cuts at: 2048 4274 5286 2048 Size: 2226 1012 2976 Fragments arranged by size: 2976 2226 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6214 BstBI TT'CG_AA Cuts at: 5018 5018 Size: 6214 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6214 BstXI CCAn_nnnn'nTGG Cuts at: 3375 3375 Size: 6214 BstYI r'GATC_y Cuts at: 1972 2861 3544 3911 4296 4313 5765 5851 5863 Size: 889 683 367 385 17 1452 86 12 Cuts at: 5863 6209 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 889 683 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2928 513 Size: 552 939 924 3799 Fragments arranged by size: 3799 939 924 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5475 Fragments arranged by size: 5475 683 56 BtsI GCAGTG_nn' Cuts at: 3730 4524 4544 3730 Size: 794 20 5400 Fragments arranged by size: 5400 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6214 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6179 DraI TTT'AAA Cuts at: 3518 3518 Size: 6214 DraIII CAC_nnn'GTG Cuts at: 3121 3121 Size: 6214 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5221 809 Size: 507 3905 1802 Fragments arranged by size: 3905 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3661 4550 6179 Size: 54 1425 50 275 18 1831 889 1629 Cuts at: 6179 8 Size: 43 Fragments arranged by size: 1831 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3661 1537 Size: 275 1849 4090 Fragments arranged by size: 4090 1849 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2275 2481 4144 1353 Size: 922 206 1663 3423 Fragments arranged by size: 3423 1663 922 206 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4801 5175 5321 387 Size: 1064 138 3212 374 146 1280 Fragments arranged by size: 3212 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3168 4277 4874 5661 5929 1239 Size: 393 1536 1109 597 787 268 1524 Fragments arranged by size: 1536 1524 1109 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2491 2491 Size: 6214 EcoRI G'AATT_C Cuts at: 3261 3523 3901 3261 Size: 262 378 5574 Fragments arranged by size: 5574 378 262 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3037 3037 Size: 6214 FspI TGC'GCA Cuts at: 4720 4720 Size: 6214 HaeII r_GCGC'y Cuts at: 1678 2084 5361 1678 Size: 406 3277 2531 Fragments arranged by size: 3277 2531 406 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2846 2878 2993 3025 4855 Size: 32 135 32 1274 32 115 32 1830 Cuts at: 4855 4887 4929 4961 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1830 1274 135 115 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3494 3558 3804 669 Size: 988 281 1556 64 246 3079 Fragments arranged by size: 3079 1556 988 281 246 64 HindIII A'AGCT_T Cuts at: 748 748 Size: 6214 HpaI GTT'AAC Cuts at: 3804 3804 Size: 6214 KpnI G_GTAC'C Cuts at: 2973 3539 2973 Size: 566 5648 MfeI C'AATT_G Cuts at: 2604 3813 2604 Size: 1209 5005 MluI A'CGCG_T Cuts at: 6090 6090 Size: 6214 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2363 5328 5512 1305 Size: 1058 2965 184 2007 Fragments arranged by size: 2965 2007 1058 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4392 Fragments arranged by size: 4392 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2432 2856 3170 3509 4172 4531 4690 Size: 648 1266 424 314 339 663 359 159 Cuts at: 4690 518 Size: 2042 Fragments arranged by size: 2042 1266 663 648 424 359 339 314 159 MspA1I CmG'CkG Cuts at: 1606 2387 3630 4307 5455 5700 1606 Size: 781 1243 677 1148 245 2120 Fragments arranged by size: 2120 1243 1148 781 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4723 Fragments arranged by size: 4723 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6214 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6214 NotI GC'GGCC_GC Cuts at: 3661 3661 Size: 6214 NspI r_CATG'y Cuts at: 1132 1229 2215 3572 5117 1132 Size: 97 986 1357 1545 2229 Fragments arranged by size: 2229 1545 1357 986 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6214 PciI A'CATG_T Cuts at: 5113 5113 Size: 6214 PflMI CCAn_nnn'nTGG Cuts at: 1108 3121 1108 Size: 2013 4201 PmeI GTTT'AAAC Cuts at: 3518 3518 Size: 6214 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4283 4315 5796 5828 4283 Size: 32 1481 32 4669 Fragments arranged by size: 4669 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2491 2491 Size: 6214 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6214 PsiI TTA'TAA Cuts at: 3784 3784 Size: 6214 PstI C_TGCA'G Cuts at: 830 3566 830 Size: 2736 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6214 PvuII CAG'CTG Cuts at: 2387 2387 Size: 6214 SalI G'TCGA_C Cuts at: 1936 3556 1936 Size: 1620 4594 SbfI CC_TGCA'GG Cuts at: 3566 3566 Size: 6214 ScaI AGT'ACT Cuts at: 4462 6174 4462 Size: 1712 4502 SfcI C'TryA_G Cuts at: 826 1045 3562 4697 5378 5569 826 Size: 219 2517 1135 681 191 1471 Fragments arranged by size: 2517 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6214 SmaI CCC'GGG Cuts at: 1872 2091 3541 1872 Size: 219 1450 4545 Fragments arranged by size: 4545 1450 219 SmlI C'TyrA_G Cuts at: 820 1017 1943 2241 3394 4318 5219 5481 820 Size: 197 926 298 1153 924 901 262 1553 Fragments arranged by size: 1553 1153 926 924 901 298 262 197 SnaBI TAC'GTA Cuts at: 493 4005 493 Size: 3512 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6214 SphI G_CATG'C Cuts at: 1132 3572 1132 Size: 2440 3774 SspI AAT'ATT Cuts at: 5 52 4138 5 Size: 47 4086 2081 Fragments arranged by size: 4086 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6214 StyI C'CwwG_G Cuts at: 513 1065 2004 2365 2974 3653 513 Size: 552 939 361 609 679 3074 Fragments arranged by size: 3074 939 679 609 552 361 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4241 4258 4411 4596 5041 325 Size: 1255 2661 17 153 185 445 1498 Fragments arranged by size: 2661 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2126 4460 6172 Size: 274 80 33 51 1443 149 2334 1712 Cuts at: 6172 96 Size: 138 Fragments arranged by size: 2334 1712 1443 274 149 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6174 6206 6174 Size: 32 6182 Tth111I GACn'n_nGTC Cuts at: 1316 2560 1316 Size: 1244 4970 XbaI T'CTAG_A Cuts at: 2711 3550 2711 Size: 839 5375 XhoI C'TCGA_G Cuts at: 1943 2241 1943 Size: 298 5916 XmaI C'CCGG_G Cuts at: 1870 2089 3539 1870 Size: 219 1450 4545 Fragments arranged by size: 4545 1450 219 XmnI GAAnn'nnTTC Cuts at: 4343 4343 Size: 6214 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4022 276 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI