Restriction map for
pFN21AB3513
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AviII BcgI BlpI
Bpu1102I BseAI BsiHKAI BsiWI BsmI
Bsp1407I BspEI BsrFI BsrGI BssHII
Bst1107I BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I CpoI DraI DraIII
Eam1105I Eco47III Eco81I EcoO65I EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI KspI MluI
MroI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 775 GTCTAC GT^[AC][GT]AC
AccII 218 CGCG CG^CG
AflII 1327 CTTAAG C^TTAAG
AflIII 1169 ACATGT A^C[GA][CT]GT
AvaI 2353 CCCGAG C^[CT]CG[GA]G
AvrII 1656 CCTAGG C^CTAGG
BalI 1010 TGGCCA TGG^CCA
BamHI 1538 GGATCC G^GATCC
BbeI 1525 GGCGCC GGCGC^C
BbrPI 761 CACGTG CAC^GTG
BciVI 2940 GTATCC GTATCC(6/5)
BcnI 180 CCGGG CC^[GC]GG
BfrI 1327 CTTAAG C^TTAAG
BglI 3035 GCCCTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2855 AGATCT A^GATCT
BlnI 1656 CCTAGG C^CTAGG
BsaAI 761 CACGTG [CT]AC^GT[GA]
BsaBI 185 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1151 ACCGGA [AT]^CCGG[AT]
BsiEI 1790 CGGCCG CG[GA][CT]^CG
BsmBI 712 GAGACG CGTCTC(1/5)
BsoBI 2353 CCCGAG C^[CT]CG[GA]G
BspDI 1738 ATCGAT AT^CGAT
BspHI 2492 TCATGA T^CATGA
BspLU11I 1169 ACATGT A^CATGT
BsrBI 2539 GAGCGG CCGCTC(-3/-3)
BssSI 197 CTCGTG CACGAG(-5/-1)
BstAPI 1342 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 515 TTCGAA TT^CGAA
BstUI 218 CGCG CG^CG
ClaI 1738 ATCGAT AT^CGAT
DrdI 1095 GACCTTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1790 CGGCCG C^GGCCG
EclXI 1790 CGGCCG C^GGCCG
Eco52I 1790 CGGCCG C^GGCCG
EcoNI 1587 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1525 GGCGCC [GA]GCGC^[CT]
KasI 1525 GGCGCC G^GCGCC
MamI 185 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1010 TGGCCA TGG^CCA
MscI 1010 TGGCCA TGG^CCA
MvnI 218 CGCG CG^CG
NarI 1525 GGCGCC GG^CGCC
NciI 180 CCGGG CC^[GC]GG
NspI 1169 ACATGT [GA]CATG^[CT]
NspV 515 TTCGAA TT^CGAA
PflMI 2580 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 761 CACGTG CAC^GTG
PmlI 761 CACGTG CAC^GTG
PpuMI 2074 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 2492 TCATGA T^CATGA
SexAI 2956 ACCTGGT A^CC[AT]GGT
SfoI 1525 GGCGCC GGC^GCC
SfuI 515 TTCGAA TT^CGAA
StuI 1982 AGGCCT AGG^CCT
Van91I 2580 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 1582 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
2581 CAGGCCCTG
Asp700I 1687 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2625 GAACGGCTTC
BanI 754 GGCACC G^G[CT][GA]CC
1525 GGCGCC
BanII 1498 GAGCCC G[GA]GC[CT]^C
1759 GGGCTC
BbvCI 496 CCTCAGC CCTCAGC(-5/-2)
2083 CCTCAGC
BclI 126 TGATCA T^GATCA
2546 TGATCA
BsgI 200 GTGCAG GTGCAG(16/14)
2785 GTGCAG
BspMI 2749 ACCTGC ACCTGC(4/8)
1273 GCAGGT
CfoI 217 GCGC GCG^C
1526 GCGC
EaeI 1010 TGGCCA [CT]^GGCC[GA]
1790 CGGCCG
EcoRI 593 GAATTC G^AATTC
2823 GAATTC
FauI 1937 CCCGC CCCGC(4/6)
1332 GCGGG
FbaI 126 TGATCA T^GATCA
2546 TGATCA
HgaI 1606 GACGC GACGC(5/10)
3032 GACGC
HhaI 217 GCGC GCG^C
1526 GCGC
HinP1I 217 GCGC G^CGC
1526 GCGC
MfeI 741 CAATTG C^AATTG
2270 CAATTG
MunI 741 CAATTG C^AATTG
2270 CAATTG
PstI 35 CTGCAG CTGCA^G
854 CTGCAG
XmnI 1687 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2625 GAACGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspI AvaII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
Hin1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PflFI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XhoII