Restriction map for
pFN21AB3499
##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AclI AflII AgeI Aor51HI AscI
AseI AsnI AspI AviII BbrPI
BciVI BfrI BlpI Bpu1102I BsiWI
Bsp1407I BspDI BspHI BsrGI BstBI
CelII ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoRV FseI FspI HindIII HpaI
MluI NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SbfI ScaI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 12 GACGTC GACGT^C
Acc65I 909 GGTACC G^GTACC
AccIII 2027 TCCGGA T^CCGGA
AhdI 2311 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1848 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 909 GGTACC G^GTACC
AspEI 2311 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2838 CCTAGG C^CTAGG
BbeI 804 GGCGCC GGCGC^C
BclI 2772 TGATCA T^GATCA
BglII 2570 AGATCT A^GATCT
BlnI 2838 CCTAGG C^CTAGG
BsaBI 255 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2027 TCCGGA T^CCGGA
BsiEI 1046 CGACCG CG[GA][CT]^CG
BspEI 2027 TCCGGA T^CCGGA
BspLU11I 2355 ACATGT A^CATGT
BsrDI 2955 GCAATG GCAATG(2/0)
BssSI 1780 CACGAG CACGAG(-5/-1)
Bst1107I 433 GTATAC GTA^TAC
BstAPI 1967 GCATCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 433 GTATAC GTA^TAC
Eam1105I 2311 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2152 GAATTC G^AATTC
EcoT22I 3200 ATGCAT ATGCA^T
FbaI 2772 TGATCA T^GATCA
KasI 804 GGCGCC G^GCGCC
KpnI 909 GGTACC GGTAC^C
MamI 255 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2756 CAATTG C^AATTG
MroI 2027 TCCGGA T^CCGGA
MunI 2756 CAATTG C^AATTG
NarI 804 GGCGCC GG^CGCC
NdeI 1520 CATATG CA^TATG
NsiI 3200 ATGCAT ATGCA^T
NspI 2355 ACATGT [GA]CATG^[CT]
PflMI 2901 CCACCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1011 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 490 GAGCTC GAGCT^C
SalI 826 GTCGAC G^TCGAC
SapI 2877 GCTCTTC GCTCTTC(1/4)
SexAI 2105 ACCAGGT A^CC[AT]GGT
SfoI 804 GGCGCC GGC^GCC
SgrAI 738 CGCCGGCG C[GA]^CCGG[CT]G
StuI 2042 AGGCCT AGG^CCT
Van91I 2901 CCACCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1848 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 1181 ACGTGT A^C[GA][CT]GT
2355 ACATGT
Alw44I 726 GTGCAC G^TGCAC
733 GTGCAC
AlwNI 1916 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
2587 CAGTATCTG
ApaLI 726 GTGCAC G^TGCAC
733 GTGCAC
BcgI 2297 CGAGTGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1904 GCACGGTGATCG
BsaAI 222 CACGTA [CT]AC^GT[GA]
2713 TACGTG
BsmBI 1941 CGTCTC CGTCTC(1/5)
1569 GAGACG
BsmI 524 GCATTC GAATGC(1/-1)
1326 GCATTC
BssHII 743 GCGCGC G^CGCGC
745 GCGCGC
BstEII 777 GGTGACC G^GT[ACGT]ACC
1362 GGTCACC
BstPI 777 GGTGACC G^GT[ACGT]ACC
1362 GGTCACC
EcoO65I 777 GGTGACC G^GT[ACGT]ACC
1362 GGTCACC
HaeII 804 GGCGCC [GA]GCGC^[CT]
838 AGCGCC
HincII 826 GTCGAC GT[CT]^[GA]AC
2344 GTCAAC
HindII 826 GTCGAC GT[CT]^[GA]AC
2344 GTCAAC
NcoI 2096 CCATGG C^CATGG
2540 CCATGG
PaeR7I 93 CTCGAG C^TCGAG
3146 CTCGAG
XhoI 93 CTCGAG C^TCGAG
3146 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PleI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI