(Circular) (Six-base) MAPSORT of: pFN21AB3499.seq Check: 8964 from: 1 to: 7921 pFN21AB3499 7921 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3222 3612 3683 3836 3222 Size: 390 71 153 7307 Fragments arranged by size: 7307 390 153 71 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2021 5731 278 Size: 53 83 186 936 485 3710 2468 Fragments arranged by size: 3710 2468 936 485 186 83 53 AccI GT'mk_AC Cuts at: 1937 2367 2439 2832 5264 1937 Size: 430 72 393 2432 4594 Fragments arranged by size: 4594 2432 430 393 72 Acc65I G'GTAC_C Cuts at: 2914 5242 2914 Size: 2328 5593 AclI AA'CG_TT Cuts at: 1563 6048 6421 1563 Size: 4485 373 3063 Fragments arranged by size: 4485 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4361 4916 5984 7368 7636 1632 Size: 2729 555 1068 1384 268 1917 Fragments arranged by size: 2729 1917 1384 1068 555 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7724 AflIII A'CryG_T Cuts at: 1203 3186 4360 6820 7797 1203 Size: 1983 1174 2460 977 1327 Fragments arranged by size: 2460 1983 1327 1174 977 AgeI A'CCGG_T Cuts at: 6752 6752 Size: 7921 AhdI GACnn_n'nnGTC Cuts at: 4321 6650 4321 Size: 2329 5592 AleI CACnn'nnGTG Cuts at: 1166 2879 4265 1166 Size: 1713 1386 4822 Fragments arranged by size: 4822 1713 1386 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2168 2258 3059 1184 Size: 984 90 801 6046 Fragments arranged by size: 6046 984 801 90 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4100 4132 4100 Size: 32 7889 AlwNI CAG_nnn'CTG Cuts at: 3926 4597 5664 7236 3926 Size: 671 1067 1572 4611 Fragments arranged by size: 4611 1572 1067 671 ApaI G_GGCC'C Cuts at: 2689 2980 3155 4540 2689 Size: 291 175 1385 6070 Fragments arranged by size: 6070 1385 291 175 ApaLI G'TGCA_C Cuts at: 2731 2738 5978 7134 2731 Size: 7 3240 1156 3518 Fragments arranged by size: 3518 3240 1156 7 ApoI r'AATT_y Cuts at: 1493 4157 4205 5196 5230 5456 5608 7717 1493 Size: 2664 48 991 34 226 152 2109 1697 Fragments arranged by size: 2664 2109 1697 991 226 152 48 34 AseI AT'TA_AT Cuts at: 160 1032 6475 160 Size: 872 5443 1606 Fragments arranged by size: 5443 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7921 AvaI C'yCGr_G Cuts at: 1870 1943 2098 2490 2963 3388 3801 4300 4692 Size: 73 155 392 473 425 413 499 392 Cuts at: 4692 5151 5246 1870 Size: 459 95 4545 Fragments arranged by size: 4545 499 473 459 425 413 392 392 155 95 73 AvrII C'CTAG_G Cuts at: 4843 4843 Size: 7921 BamHI G'GATC_C Cuts at: 3393 3883 4144 5251 5618 3393 Size: 490 261 1107 367 5696 Fragments arranged by size: 5696 1107 490 367 261 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2024 2191 2552 2770 2809 Size: 325 217 627 237 167 361 218 39 Cuts at: 2809 2914 3341 3824 4076 5242 6697 618 Size: 105 427 483 252 1166 1455 1842 Fragments arranged by size: 1842 1455 1166 627 483 427 361 325 252 237 218 217 167 105 39 BanII G_rGCy'C Cuts at: 1391 2499 2689 2980 3155 3253 3611 3801 3870 Size: 1108 190 291 175 98 358 190 69 Cuts at: 3870 4540 5158 5240 1391 Size: 670 618 82 4072 Fragments arranged by size: 4072 1108 670 618 358 291 190 190 175 98 82 69 BbeI G_GCGC'C Cuts at: 2813 2813 Size: 7921 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2371 2890 3879 4904 928 Size: 971 472 519 989 1025 3945 Fragments arranged by size: 3945 1025 989 971 519 472 BbvCI CC'TCA_GC Cuts at: 3373 4015 4653 3373 Size: 642 638 6641 Fragments arranged by size: 6641 642 638 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3898 3932 4291 4325 6112 6146 1903 Size: 34 1961 34 359 34 1787 34 3678 Fragments arranged by size: 3678 1961 1787 359 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5813 7023 5813 Size: 1210 6711 BclI T'GATC_A Cuts at: 1548 4777 1548 Size: 3229 4692 BfrBI ATG'CAT Cuts at: 5207 5207 Size: 7921 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3539 3705 3765 Size: 107 122 71 631 605 1867 166 60 Cuts at: 3765 4395 6532 136 Size: 630 2137 1525 Fragments arranged by size: 2137 1867 1525 631 630 605 166 122 107 71 60 BglII A'GATC_T Cuts at: 4575 7916 4575 Size: 3341 4580 BlpI GC'TnA_GC Cuts at: 5338 5338 Size: 7921 Bme1580I G_kGCm'C Cuts at: 1790 2196 2434 2597 2689 2735 2742 2775 2980 Size: 406 238 163 92 46 7 33 205 Cuts at: 2980 3155 4081 4540 5982 7138 1790 Size: 175 926 459 1442 1156 2573 Fragments arranged by size: 2573 1442 1156 926 459 406 238 205 175 163 92 46 33 7 BmgBI CAC'GTC Cuts at: 1318 2911 1318 Size: 1593 6328 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2122 3213 5652 Size: 416 53 83 399 24 705 1091 2439 Cuts at: 5652 6610 442 Size: 958 1753 Fragments arranged by size: 2439 1753 1091 958 705 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7921 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2821 3403 4312 2821 Size: 582 909 6430 Fragments arranged by size: 6430 909 582 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2645 2687 3602 4160 4492 5039 6581 1239 Size: 1406 42 915 558 332 547 1542 2579 Fragments arranged by size: 2579 1542 1406 915 558 547 332 42 Bpu10I CC'TnA_GC Cuts at: 3373 4015 4653 4875 3373 Size: 642 638 222 6419 Fragments arranged by size: 6419 642 638 222 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2357 2468 6046 6911 7209 2357 Size: 111 3578 865 298 3069 Fragments arranged by size: 3578 3069 865 298 111 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3008 3719 4120 4308 6584 882 Size: 617 1509 711 401 188 2276 2219 Fragments arranged by size: 2276 2219 1509 711 617 401 188 BsaAI yAC'GTr Cuts at: 493 1204 2229 4720 5712 493 Size: 711 1025 2491 992 2702 Fragments arranged by size: 2702 2491 1025 992 711 BsaBI GATnn'nnATC Cuts at: 2264 7915 2264 Size: 5651 2270 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2018 2178 2810 2829 Size: 53 83 186 936 485 160 632 19 Cuts at: 2829 3165 5063 5728 6110 6745 275 Size: 336 1898 665 382 635 1451 Fragments arranged by size: 1898 1451 936 665 635 632 485 382 336 186 160 83 53 19 BsaWI w'CCGG_w Cuts at: 2021 2298 3081 4032 4073 4908 6354 6752 7026 Size: 277 783 951 41 835 1446 398 274 Cuts at: 7026 7173 7584 2021 Size: 147 411 2358 Fragments arranged by size: 2358 1446 951 835 783 411 398 277 274 147 41 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2108 2138 2300 2330 3150 Size: 30 165 30 705 30 162 30 820 Cuts at: 3150 3180 3218 3248 3664 3694 3839 3869 4100 Size: 30 38 30 416 30 145 30 231 Cuts at: 4100 4130 4496 4526 4720 4750 1178 Size: 30 366 30 194 30 4349 Fragments arranged by size: 4349 820 705 416 366 231 194 165 162 145 38 30 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2201 2276 2771 3551 3605 3860 3863 4003 Size: 1015 75 495 780 54 255 3 140 Cuts at: 4003 4057 4394 4397 4741 4952 5053 5123 5126 Size: 54 337 3 344 211 101 70 3 Cuts at: 5126 1186 Size: 3981 Fragments arranged by size: 3981 1015 780 495 344 337 255 211 140 101 75 70 54 54 3 3 3 BseYI C'CCAG_C Cuts at: 2195 2682 2979 3498 3683 7124 7620 2195 Size: 487 297 519 185 3441 496 2496 Fragments arranged by size: 3441 2496 519 496 487 297 185 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2603 3194 3582 1163 Size: 573 867 591 388 5502 Fragments arranged by size: 5502 867 591 573 388 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3054 5371 6132 7160 1540 Size: 275 186 1053 2317 761 1028 2301 Fragments arranged by size: 2317 2301 1053 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2499 2678 2735 2742 5982 6067 7138 2499 Size: 179 57 7 3240 85 1071 3282 Fragments arranged by size: 3282 3240 1071 179 85 57 7 BsmI GAATG_Cn' Cuts at: 2529 3331 5432 5525 2529 Size: 802 2101 93 4925 Fragments arranged by size: 4925 2101 802 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3568 3952 3568 Size: 384 7537 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2196 2434 2499 2597 2678 2689 2735 Size: 399 406 238 65 98 81 11 46 Cuts at: 2735 2742 2775 2980 3155 3253 3611 3801 3870 Size: 7 33 205 175 98 358 190 69 Cuts at: 3870 4081 4540 5158 5240 5982 6067 7138 1391 Size: 211 459 618 82 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 618 459 406 399 358 238 211 205 190 175 98 98 85 82 81 69 65 46 33 11 7 BspEI T'CCGG_A Cuts at: 4032 7584 4032 Size: 3552 4369 BspHI T'CATG_A Cuts at: 5810 7540 5810 Size: 1730 6191 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2926 3222 3612 3683 3704 3836 4326 4336 Size: 2082 296 390 71 21 132 490 10 Cuts at: 4336 5276 844 Size: 940 3489 Fragments arranged by size: 3489 2082 940 490 390 296 132 71 21 10 BsrBI CCG'CTC Cuts at: 1394 1823 3161 4402 4534 4879 5808 1394 Size: 429 1338 1241 132 345 929 3507 Fragments arranged by size: 3507 1338 1241 929 429 345 132 BsrDI GCAATG_nn' Cuts at: 65 1248 4967 6416 6590 65 Size: 1183 3719 1449 174 1396 Fragments arranged by size: 3719 1449 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2141 2279 2744 3199 4083 4901 4990 Size: 110 331 138 465 455 884 818 89 Cuts at: 4990 6565 6752 1700 Size: 1575 187 2869 Fragments arranged by size: 2869 1575 884 818 465 455 331 187 138 110 89 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7921 BssHII G'CGCG_C Cuts at: 1925 2748 2750 1925 Size: 823 2 7096 Fragments arranged by size: 7096 823 2 BssSI C'ACGA_G Cuts at: 3785 5981 6993 3785 Size: 2196 1012 4713 Fragments arranged by size: 4713 2196 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3978 794 Size: 3184 4737 BstBI TT'CG_AA Cuts at: 6725 6725 Size: 7921 BstEII G'GTnAC_C Cuts at: 1184 2782 3367 1184 Size: 1598 585 5738 Fragments arranged by size: 5738 1598 585 BstXI CCAn_nnnn'nTGG Cuts at: 2848 3118 4516 4752 2848 Size: 270 1398 236 6017 Fragments arranged by size: 6017 1398 270 236 BstYI r'GATC_y Cuts at: 1972 2259 3393 3883 4134 4144 4575 5251 5618 Size: 287 1134 490 251 10 431 676 367 Cuts at: 5618 6003 6020 7472 7558 7570 7916 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1134 676 490 431 385 367 346 287 251 86 17 12 10 BstZ17I GTA'TAC Cuts at: 2440 2440 Size: 7921 Bsu36I CC'TnA_GG Cuts at: 2184 3750 3843 2184 Size: 1566 93 6262 Fragments arranged by size: 6262 1566 93 BtgI C'CryG_G Cuts at: 513 1065 2004 2028 2344 2823 2847 2877 4101 Size: 552 939 24 316 479 24 30 1224 Cuts at: 4101 4119 4263 4545 513 Size: 18 144 282 3889 Fragments arranged by size: 3889 1224 939 552 479 316 282 144 30 24 24 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3729 487 Size: 683 56 2503 4679 Fragments arranged by size: 4679 2503 683 56 BtsI GCAGTG_nn' Cuts at: 3709 3976 4495 5437 6231 6251 3709 Size: 267 519 942 794 20 5379 Fragments arranged by size: 5379 942 794 519 267 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7921 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5155 5190 529 Size: 35 4591 35 3260 Fragments arranged by size: 4591 3260 35 35 DraI TTT'AAA Cuts at: 5225 5225 Size: 7921 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6928 809 Size: 507 5612 1802 Fragments arranged by size: 5612 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2420 2724 2883 Size: 54 1425 50 275 18 590 304 159 Cuts at: 2883 3462 3531 3706 4269 4599 4903 5368 6257 Size: 579 69 175 563 330 304 465 889 Cuts at: 6257 7886 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 590 579 563 465 330 304 304 275 175 159 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5368 1537 Size: 275 3556 4090 Fragments arranged by size: 4090 3556 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3938 4075 4701 4718 4889 5851 1353 Size: 2585 137 626 17 171 962 3423 Fragments arranged by size: 3423 2585 962 626 171 137 17 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2192 2558 4675 6508 6882 7028 Size: 1064 138 603 366 2117 1833 374 146 Cuts at: 7028 387 Size: 1280 Fragments arranged by size: 2117 1833 1280 1064 603 374 366 146 138 EcoICRI GAG'CTC Cuts at: 2497 2497 Size: 7921 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2645 2687 3602 4160 4361 4492 4916 Size: 393 1013 42 915 558 201 131 424 Cuts at: 4916 5039 5984 6581 7368 7636 1239 Size: 123 945 597 787 268 1524 Fragments arranged by size: 1524 1013 945 915 787 597 558 424 393 268 201 131 123 42 EcoNI CCTnn'n_nnAGG Cuts at: 2402 2922 4033 4343 5105 2402 Size: 520 1111 310 762 5218 Fragments arranged by size: 5218 1111 762 520 310 EcoO109I rG'GnC_Cy Cuts at: 2517 2686 3151 3418 4380 4472 4537 2517 Size: 169 465 267 962 92 65 5901 Fragments arranged by size: 5901 962 465 267 169 92 65 EcoRI G'AATT_C Cuts at: 4157 5230 5608 4157 Size: 1073 378 6470 Fragments arranged by size: 6470 1073 378 FspI TGC'GCA Cuts at: 6427 6427 Size: 7921 HaeII r_GCGC'y Cuts at: 1678 2813 2847 7068 1678 Size: 1135 34 4221 2531 Fragments arranged by size: 4221 2531 1135 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2300 2332 3150 3182 4307 Size: 32 135 32 728 32 818 32 1125 Cuts at: 4307 4339 4544 4576 4579 4611 4720 4752 4793 Size: 32 205 32 3 32 109 32 41 Cuts at: 4793 4825 5087 5119 6562 6594 6636 6668 1373 Size: 32 262 32 1443 32 42 32 2626 Fragments arranged by size: 2626 1443 1125 818 728 262 205 135 109 42 41 32 32 32 32 32 32 32 32 32 32 32 32 3 HincII GTy'rAC Cuts at: 669 1657 1938 2833 4351 5265 5511 669 Size: 988 281 895 1518 914 246 3079 Fragments arranged by size: 3079 1518 988 914 895 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7921 HpaI GTT'AAC Cuts at: 5511 5511 Size: 7921 KasI G'GCGC_C Cuts at: 2809 2809 Size: 7921 KpnI G_GTAC'C Cuts at: 2918 5246 2918 Size: 2328 5593 MfeI C'AATT_G Cuts at: 4761 5520 4761 Size: 759 7162 MluI A'CGCG_T Cuts at: 7797 7797 Size: 7921 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4718 7035 7219 1305 Size: 3413 2317 184 2007 Fragments arranged by size: 3413 2317 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2422 2885 3464 10 Size: 54 1425 343 590 463 579 4467 Fragments arranged by size: 4467 1425 590 579 463 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2606 2879 3458 3714 4265 4359 4527 Size: 648 1440 273 579 256 551 94 168 Cuts at: 4527 5879 6238 6397 518 Size: 1352 359 159 2042 Fragments arranged by size: 2042 1440 1352 648 579 551 359 273 256 168 159 94 MspA1I CmG'CkG Cuts at: 1606 2030 2065 2346 2649 2704 2825 3118 3502 Size: 424 35 281 303 55 121 293 384 Cuts at: 3502 3926 4019 5122 5337 6014 7162 7407 1606 Size: 424 93 1103 215 677 1148 245 2120 Fragments arranged by size: 2120 1148 1103 677 424 424 384 303 293 281 245 215 121 93 55 35 NaeI GCC'GGC Cuts at: 2143 2746 4085 2143 Size: 603 1339 5979 Fragments arranged by size: 5979 1339 603 NarI GG'CG_CC Cuts at: 2810 2810 Size: 7921 NcoI C'CATG_G Cuts at: 513 1065 2004 4101 4545 513 Size: 552 939 2097 444 3889 Fragments arranged by size: 3889 2097 939 552 444 NdeI CA'TA_TG Cuts at: 387 3526 387 Size: 3139 4782 NgoMIV G'CCGG_C Cuts at: 2141 2744 4083 2141 Size: 603 1339 5979 Fragments arranged by size: 5979 1339 603 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7921 NotI GC'GGCC_GC Cuts at: 5368 5368 Size: 7921 NsiI A_TGCA'T Cuts at: 5209 5209 Size: 7921 NspI r_CATG'y Cuts at: 1132 1229 4364 5279 6824 1132 Size: 97 3135 915 1545 2229 Fragments arranged by size: 3135 2229 1545 915 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7921 PciI A'CATG_T Cuts at: 4360 6820 4360 Size: 2460 5461 PflMI CCAn_nnn'nTGG Cuts at: 1108 4912 1108 Size: 3804 4117 PfoI T'CCnGG_A Cuts at: 2268 2622 3879 4137 2268 Size: 354 1257 258 6052 Fragments arranged by size: 6052 1257 354 258 PmeI GTTT'AAAC Cuts at: 5225 5225 Size: 7921 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4100 4132 5990 6022 7503 7535 4100 Size: 32 1858 32 1481 32 4486 Fragments arranged by size: 4486 1858 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3020 1613 Size: 1407 6514 PsiI TTA'TAA Cuts at: 5491 5491 Size: 7921 PspOMI G'GGCC_C Cuts at: 2685 2976 3151 4536 2685 Size: 291 175 1385 6070 Fragments arranged by size: 6070 1385 291 175 PspXI vC'TCGA_Gb Cuts at: 5151 5151 Size: 7921 PstI C_TGCA'G Cuts at: 830 2477 3353 4388 4934 5273 830 Size: 1647 876 1035 546 339 3478 Fragments arranged by size: 3478 1647 1035 876 546 339 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7921 PvuII CAG'CTG Cuts at: 3118 3502 4019 3118 Size: 384 517 7020 Fragments arranged by size: 7020 517 384 SacI G_AGCT'C Cuts at: 2499 2499 Size: 7921 SacII CC_GC'GG Cuts at: 2031 2347 2826 2031 Size: 316 479 7126 Fragments arranged by size: 7126 479 316 SalI G'TCGA_C Cuts at: 1936 2831 5263 1936 Size: 895 2432 4594 Fragments arranged by size: 4594 2432 895 SapI GCTCTTCn'nnn_ Cuts at: 4889 4889 Size: 7921 SbfI CC_TGCA'GG Cuts at: 5273 5273 Size: 7921 ScaI AGT'ACT Cuts at: 6169 7881 6169 Size: 1712 6209 SexAI A'CCwGG_T Cuts at: 4110 4110 Size: 7921 SfcI C'TryA_G Cuts at: 826 1045 2473 2614 3064 3349 4384 4930 5269 Size: 219 1428 141 450 285 1035 546 339 Cuts at: 5269 6404 7085 7276 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1428 1135 1035 681 546 450 339 285 219 191 141 SfoI GGC'GCC Cuts at: 2811 2811 Size: 7921 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7921 SgrAI Cr'CCGG_yG Cuts at: 1700 2744 1700 Size: 1044 6877 SmaI CCC'GGG Cuts at: 1872 2492 2965 3803 5248 1872 Size: 620 473 838 1445 4545 Fragments arranged by size: 4545 1445 838 620 473 SmlI C'TyrA_G Cuts at: 820 1017 1943 2098 2372 2483 5151 6025 6926 Size: 197 926 155 274 111 2668 874 901 Cuts at: 6926 7188 820 Size: 262 1553 Fragments arranged by size: 2668 1553 926 901 874 274 262 197 155 111 SnaBI TAC'GTA Cuts at: 493 5712 493 Size: 5219 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7921 SphI G_CATG'C Cuts at: 1132 5279 1132 Size: 4147 3774 SspI AAT'ATT Cuts at: 5 52 5845 5 Size: 47 5793 2081 Fragments arranged by size: 5793 2081 47 StuI AGG'CCT Cuts at: 1517 4049 1517 Size: 2532 5389 StyI C'CwwG_G Cuts at: 513 1065 2004 3414 4101 4545 4843 5360 513 Size: 552 939 1410 687 444 298 517 3074 Fragments arranged by size: 3074 1410 939 687 552 517 444 298 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2404 3010 4021 4371 5948 5965 6118 Size: 1255 824 606 1011 350 1577 17 153 Cuts at: 6118 6303 6748 325 Size: 185 445 1498 Fragments arranged by size: 1577 1498 1255 1011 824 606 445 350 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3321 6167 7879 Size: 274 80 33 51 1443 1344 2846 1712 Cuts at: 7879 96 Size: 138 Fragments arranged by size: 2846 1712 1443 1344 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2449 2481 3265 3297 3664 3696 3839 3871 3875 Size: 32 784 32 367 32 143 32 4 Cuts at: 3875 3907 4136 4168 7881 7913 2449 Size: 32 229 32 3713 32 2457 Fragments arranged by size: 3713 2457 784 367 229 143 32 32 32 32 32 32 32 4 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7921 XbaI T'CTAG_A Cuts at: 5257 5257 Size: 7921 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2203 2605 2848 3302 4642 2203 Size: 402 243 454 1340 5482 Fragments arranged by size: 5482 1340 454 402 243 XhoI C'TCGA_G Cuts at: 1943 2098 5151 1943 Size: 155 3053 4713 Fragments arranged by size: 4713 3053 155 XmaI C'CCGG_G Cuts at: 1870 2490 2963 3801 5246 1870 Size: 620 473 838 1445 4545 Fragments arranged by size: 4545 1445 838 620 473 XmnI GAAnn'nnTTC Cuts at: 3857 6050 3857 Size: 2193 5728 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2019 5729 276 Size: 53 83 186 936 485 3710 2468 Fragments arranged by size: 3710 2468 936 485 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BsiWI DraIII EcoRV FalI FseI FspAI NruI PacI PmlI PpuMI PsrI RsrII SanDI SfiI SrfI SwaI