Restriction map for pFN21AB3499
 (Linear) (Six-base) MAPSORT of: KIBB3499.seq  Check: 29  from: 1  to: 3216



KIBB3499.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1217    1607    1678    1831    3216 
   Size:       1217     390      71     153    1385

  Fragments arranged by size: 

                1385    1217     390     153      71
 
AatII G_ACGT'C

Cuts at:      0      16    3216 
   Size:         16    3200
 
AccI GT'mk_AC

Cuts at:      0     362     434     827    3216 
   Size:        362      72     393    2389

  Fragments arranged by size: 

                2389     393     362      72
 
Acc65I G'GTAC_C

Cuts at:      0     909    3216 
   Size:        909    2307
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2356    2911    3216 
   Size:       2356     555     305

  Fragments arranged by size: 

                2356     555     305
 
AflIII A'CryG_T

Cuts at:      0    1181    2355    3216 
   Size:       1181    1174     861

  Fragments arranged by size: 

                1181    1174     861
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2316    3216 
   Size:       2316     900
 
AleI CACnn'nnGTG

Cuts at:      0     874    2260    3216 
   Size:        874    1386     956

  Fragments arranged by size: 

                1386     956     874
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     163     253    1054    3216 
   Size:        163      90     801    2162

  Fragments arranged by size: 

                2162     801     163      90
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2095    2127    3216 
   Size:       2095      32    1089

  Fragments arranged by size: 

                2095    1089      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1921    2592    3216 
   Size:       1921     671     624

  Fragments arranged by size: 

                1921     671     624
 
ApaI G_GGCC'C

Cuts at:      0     684     975    1150    2535    3216 
   Size:        684     291     175    1385     681

  Fragments arranged by size: 

                1385     684     681     291     175
 
ApaLI G'TGCA_C

Cuts at:      0     726     733    3216 
   Size:        726       7    2483

  Fragments arranged by size: 

                2483     726       7
 
ApoI r'AATT_y

Cuts at:      0    2152    2200    3191    3216 
   Size:       2152      48     991      25

  Fragments arranged by size: 

                2152     991      48      25
 
AvaI C'yCGr_G

Cuts at:      0      93     485     958    1383    1796    2295    2687    3146 
   Size:         93     392     473     425     413     499     392     459

Cuts at:   3146    3216 
   Size:         70

  Fragments arranged by size: 

                 499     473     459     425     413     392     392      93
                  70
 
AvrII C'CTAG_G

Cuts at:      0    2838    3216 
   Size:       2838     378
 
BamHI G'GATC_C

Cuts at:      0    1388    1878    2139    3216 
   Size:       1388     490     261    1077

  Fragments arranged by size: 

                1388    1077     490     261
 
BanI G'GyrC_C

Cuts at:      0      19     186     547     765     804     909    1336    1819 
   Size:         19     167     361     218      39     105     427     483

Cuts at:   1819    2071    3216 
   Size:        252    1145

  Fragments arranged by size: 

                1145     483     427     361     252     218     167     105
                  39      19
 
BanII G_rGCy'C

Cuts at:      0     494     684     975    1150    1248    1606    1796    1865 
   Size:        494     190     291     175      98     358     190      69

Cuts at:   1865    2535    3153    3216 
   Size:        670     618      63

  Fragments arranged by size: 

                 670     618     494     358     291     190     190     175
                  98      69      63
 
BbeI G_GCGC'C

Cuts at:      0     808    3216 
   Size:        808    2408
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     366     885    1874    2899    3216 
   Size:        366     519     989    1025     317

  Fragments arranged by size: 

                1025     989     519     366     317
 
BbvCI CC'TCA_GC

Cuts at:      0    1368    2010    2648    3216 
   Size:       1368     642     638     568

  Fragments arranged by size: 

                1368     642     638     568
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1893    1927    2286    2320    3216 
   Size:       1893      34     359      34     896

  Fragments arranged by size: 

                1893     896     359      34      34
 
BclI T'GATC_A

Cuts at:      0    2772    3216 
   Size:       2772     444
 
BfrBI ATG'CAT

Cuts at:      0    3202    3216 
   Size:       3202      14
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1534    1700    1760    2390    3216 
   Size:       1534     166      60     630     826

  Fragments arranged by size: 

                1534     826     630     166      60
 
BglII A'GATC_T

Cuts at:      0    2570    3216 
   Size:       2570     646
 
Bme1580I G_kGCm'C

Cuts at:      0     191     429     592     684     730     737     770     975 
   Size:        191     238     163      92      46       7      33     205

Cuts at:    975    1150    2076    2535    3216 
   Size:        175     926     459     681

  Fragments arranged by size: 

                 926     681     459     238     205     191     175     163
                  92      46      33       7
 
BmgBI CAC'GTC

Cuts at:      0     906    3216 
   Size:        906    2310
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     117    1208    3216 
   Size:        117    1091    2008

  Fragments arranged by size: 

                2008    1091     117
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     816    1398    2307    3216 
   Size:        816     582     909     909

  Fragments arranged by size: 

                 909     909     816     582
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     640     682    1597    2155    2487    3034    3216 
   Size:        640      42     915     558     332     547     182

  Fragments arranged by size: 

                 915     640     558     547     332     182      42
 
Bpu10I CC'TnA_GC

Cuts at:      0    1368    2010    2648    2870    3216 
   Size:       1368     642     638     222     346

  Fragments arranged by size: 

                1368     642     638     346     222
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     463    3216 
   Size:        352     111    2753

  Fragments arranged by size: 

                2753     352     111
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1003    1714    2115    2303    3216 
   Size:       1003     711     401     188     913

  Fragments arranged by size: 

                1003     913     711     401     188
 
BsaAI yAC'GTr

Cuts at:      0     224    2715    3216 
   Size:        224    2491     501

  Fragments arranged by size: 

                2491     501     224
 
BsaBI GATnn'nnATC

Cuts at:      0     259    3216 
   Size:        259    2957
 
BsaHI Gr'CG_yC

Cuts at:      0      13     173     805     824    1160    3058    3216 
   Size:         13     160     632      19     336    1898     158

  Fragments arranged by size: 

                1898     632     336     160     158      19      13
 
BsaWI w'CCGG_w

Cuts at:      0      16     293    1076    2027    2068    2903    3216 
   Size:         16     277     783     951      41     835     313

  Fragments arranged by size: 

                 951     835     783     313     277      41      16
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     103     133     295     325    1145    1175    1213    1243 
   Size:        103      30     162      30     820      30      38      30

Cuts at:   1243    1659    1689    1834    1864    2095    2125    2491    2521 
   Size:        416      30     145      30     231      30     366      30

Cuts at:   2521    2715    2745    3216 
   Size:        194      30     471

  Fragments arranged by size: 

                 820     471     416     366     231     194     162     145
                 103      38      30      30      30      30      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     196     271     766    1546    1600    1855    1858    1998 
   Size:        196      75     495     780      54     255       3     140

Cuts at:   1998    2052    2389    2392    2736    2947    3048    3118    3121 
   Size:         54     337       3     344     211     101      70       3

Cuts at:   3121    3216 
   Size:         95

  Fragments arranged by size: 

                 780     495     344     337     255     211     196     140
                 101      95      75      70      54      54       3       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0     190     677     974    1493    1678    3216 
   Size:        190     487     297     519     185    1538

  Fragments arranged by size: 

                1538     519     487     297     190     185
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598    1189    1577    3216 
   Size:        598     591     388    1639

  Fragments arranged by size: 

                1639     598     591     388
 
BsiEI CG_ry'CG

Cuts at:      0    1049    3216 
   Size:       1049    2167
 
BsiHKAI G_wGCw'C

Cuts at:      0     494     673     730     737    3216 
   Size:        494     179      57       7    2479

  Fragments arranged by size: 

                2479     494     179      57       7
 
BsmI GAATG_Cn'

Cuts at:      0     524    1326    3216 
   Size:        524     802    1890

  Fragments arranged by size: 

                1890     802     524
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1563    1947    3216 
   Size:       1563     384    1269

  Fragments arranged by size: 

                1563    1269     384
 
Bsp1286I G_dGCh'C

Cuts at:      0     191     429     494     592     673     684     730     737 
   Size:        191     238      65      98      81      11      46       7

Cuts at:    737     770     975    1150    1248    1606    1796    1865    2076 
   Size:         33     205     175      98     358     190      69     211

Cuts at:   2076    2535    3153    3216 
   Size:        459     618      63

  Fragments arranged by size: 

                 618     459     358     238     211     205     191     190
                 175      98      98      81      69      65      63      46
                  33      11       7
 
BspEI T'CCGG_A

Cuts at:      0    2027    3216 
   Size:       2027    1189
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     921    1217    1607    1678    1699    1831    2321    2331 
   Size:        921     296     390      71      21     132     490      10

Cuts at:   2331    3216 
   Size:        885

  Fragments arranged by size: 

                 921     885     490     390     296     132      71      21
                  10
 
BsrBI CCG'CTC

Cuts at:      0    1156    2397    2529    2874    3216 
   Size:       1156    1241     132     345     342

  Fragments arranged by size: 

                1241    1156     345     342     132
 
BsrDI GCAATG_nn'

Cuts at:      0    2962    3216 
   Size:       2962     254
 
BsrFI r'CCGG_y

Cuts at:      0     136     274     739    1194    2078    2896    2985    3216 
   Size:        136     138     465     455     884     818      89     231

  Fragments arranged by size: 

                 884     818     465     455     231     138     136      89
            
 
BssHII G'CGCG_C

Cuts at:      0     743     745    3216 
   Size:        743       2    2471

  Fragments arranged by size: 

                2471     743       2
 
BssSI C'ACGA_G

Cuts at:      0    1780    3216 
   Size:       1780    1436
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1973    3216 
   Size:       1973    1243
 
BstEII G'GTnAC_C

Cuts at:      0     777    1362    3216 
   Size:        777     585    1854

  Fragments arranged by size: 

                1854     777     585
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     843    1113    2511    2747    3216 
   Size:        843     270    1398     236     469

  Fragments arranged by size: 

                1398     843     469     270     236
 
BstYI r'GATC_y

Cuts at:      0     254    1388    1878    2129    2139    2570    3216 
   Size:        254    1134     490     251      10     431     646

  Fragments arranged by size: 

                1134     646     490     431     254     251      10
 
BstZ17I GTA'TAC

Cuts at:      0     435    3216 
   Size:        435    2781
 
Bsu36I CC'TnA_GG

Cuts at:      0     179    1745    1838    3216 
   Size:        179    1566      93    1378

  Fragments arranged by size: 

                1566    1378     179      93
 
BtgI C'CryG_G

Cuts at:      0      23     339     818     842     872    2096    2114    2258 
   Size:         23     316     479      24      30    1224      18     144

Cuts at:   2258    2540    3216 
   Size:        282     676

  Fragments arranged by size: 

                1224     676     479     316     282     144      30      24
                  23      18
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1724    3216 
   Size:       1724    1492
 
BtsI GCAGTG_nn'

Cuts at:      0    1704    1971    2490    3216 
   Size:       1704     267     519     726

  Fragments arranged by size: 

                1704     726     519     267
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3150    3185    3216 
   Size:       3150      35      31

  Fragments arranged by size: 

                3150      35      31
 
EaeI y'GGCC_r

Cuts at:      0     415     719     878    1457    1526    1701    2264    2594 
   Size:        415     304     159     579      69     175     563     330

Cuts at:   2594    2898    3216 
   Size:        304     318

  Fragments arranged by size: 

                 579     563     415     330     318     304     304     175
                 159      69
 
EarI CTCTTCn'nnn_

Cuts at:      0    1933    2070    2696    2713    2884    3216 
   Size:       1933     137     626      17     171     332

  Fragments arranged by size: 

                1933     626     332     171     137      17
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     187     553    2670    3216 
   Size:        187     366    2117     546

  Fragments arranged by size: 

                2117     546     366     187
 
EcoICRI GAG'CTC

Cuts at:      0     492    3216 
   Size:        492    2724
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     640     682    1597    2155    2356    2487    2911    3034 
   Size:        640      42     915     558     201     131     424     123

Cuts at:   3034    3216 
   Size:        182

  Fragments arranged by size: 

                 915     640     558     424     201     182     131     123
                  42
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     397     917    2028    2338    3100    3216 
   Size:        397     520    1111     310     762     116

  Fragments arranged by size: 

                1111     762     520     397     310     116
 
EcoO109I rG'GnC_Cy

Cuts at:      0     512     681    1146    1413    2375    2467    2532    3216 
   Size:        512     169     465     267     962      92      65     684

  Fragments arranged by size: 

                 962     684     512     465     267     169      92      65
            
 
EcoRI G'AATT_C

Cuts at:      0    2152    3216 
   Size:       2152    1064
 
HaeII r_GCGC'y

Cuts at:      0     808     842    3216 
   Size:        808      34    2374

  Fragments arranged by size: 

                2374     808      34
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     295     327    1145    1177    2302    2334    2539    2571 
   Size:        295      32     818      32    1125      32     205      32

Cuts at:   2571    2574    2606    2715    2747    2788    2820    3082    3114 
   Size:          3      32     109      32      41      32     262      32

Cuts at:   3114    3216 
   Size:        102

  Fragments arranged by size: 

                1125     818     295     262     205     109     102      41
                  32      32      32      32      32      32      32      32
                   3
 
HincII GTy'rAC

Cuts at:      0     828    2346    3216 
   Size:        828    1518     870

  Fragments arranged by size: 

                1518     870     828
 
KasI G'GCGC_C

Cuts at:      0     804    3216 
   Size:        804    2412
 
KpnI G_GTAC'C

Cuts at:      0     913    3216 
   Size:        913    2303
 
MfeI C'AATT_G

Cuts at:      0    2756    3216 
   Size:       2756     460
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2713    3216 
   Size:       2713     503
 
MscI TGG'CCA

Cuts at:      0     417     880    1459    3216 
   Size:        417     463     579    1757

  Fragments arranged by size: 

                1757     579     463     417
 
MslI CAynn'nnrTG

Cuts at:      0     601     874    1453    1709    2260    2354    2522    3216 
   Size:        601     273     579     256     551      94     168     694

  Fragments arranged by size: 

                 694     601     579     551     273     256     168      94
            
 
MspA1I CmG'CkG

Cuts at:      0      25      60     341     644     699     820    1113    1497 
   Size:         25      35     281     303      55     121     293     384

Cuts at:   1497    1921    2014    3117    3216 
   Size:        424      93    1103      99

  Fragments arranged by size: 

                1103     424     384     303     293     281     121      99
                  93      55      35      25
 
NaeI GCC'GGC

Cuts at:      0     138     741    2080    3216 
   Size:        138     603    1339    1136

  Fragments arranged by size: 

                1339    1136     603     138
 
NarI GG'CG_CC

Cuts at:      0     805    3216 
   Size:        805    2411
 
NcoI C'CATG_G

Cuts at:      0    2096    2540    3216 
   Size:       2096     444     676

  Fragments arranged by size: 

                2096     676     444
 
NdeI CA'TA_TG

Cuts at:      0    1521    3216 
   Size:       1521    1695
 
NgoMIV G'CCGG_C

Cuts at:      0     136     739    2078    3216 
   Size:        136     603    1339    1138

  Fragments arranged by size: 

                1339    1138     603     136
 
NsiI A_TGCA'T

Cuts at:      0    3204    3216 
   Size:       3204      12
 
NspI r_CATG'y

Cuts at:      0    2359    3216 
   Size:       2359     857
 
PciI A'CATG_T

Cuts at:      0    2355    3216 
   Size:       2355     861
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2907    3216 
   Size:       2907     309
 
PfoI T'CCnGG_A

Cuts at:      0     263     617    1874    2132    3216 
   Size:        263     354    1257     258    1084

  Fragments arranged by size: 

                1257    1084     354     263     258
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2095    2127    3216 
   Size:       2095      32    1089

  Fragments arranged by size: 

                2095    1089      32
 
PshAI GACnn'nnGTC

Cuts at:      0    1015    3216 
   Size:       1015    2201
 
PspOMI G'GGCC_C

Cuts at:      0     680     971    1146    2531    3216 
   Size:        680     291     175    1385     685

  Fragments arranged by size: 

                1385     685     680     291     175
 
PspXI vC'TCGA_Gb

Cuts at:      0    3146    3216 
   Size:       3146      70
 
PstI C_TGCA'G

Cuts at:      0     472    1348    2383    2929    3216 
   Size:        472     876    1035     546     287

  Fragments arranged by size: 

                1035     876     546     472     287
 
PvuII CAG'CTG

Cuts at:      0    1113    1497    2014    3216 
   Size:       1113     384     517    1202

  Fragments arranged by size: 

                1202    1113     517     384
 
SacI G_AGCT'C

Cuts at:      0     494    3216 
   Size:        494    2722
 
SacII CC_GC'GG

Cuts at:      0      26     342     821    3216 
   Size:         26     316     479    2395

  Fragments arranged by size: 

                2395     479     316      26
 
SalI G'TCGA_C

Cuts at:      0     826    3216 
   Size:        826    2390
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2884    3216 
   Size:       2884     332
 
SexAI A'CCwGG_T

Cuts at:      0    2105    3216 
   Size:       2105    1111
 
SfcI C'TryA_G

Cuts at:      0     468     609    1059    1344    2379    2925    3216 
   Size:        468     141     450     285    1035     546     291

  Fragments arranged by size: 

                1035     546     468     450     291     285     141
 
SfoI GGC'GCC

Cuts at:      0     806    3216 
   Size:        806    2410
 
SgrAI Cr'CCGG_yG

Cuts at:      0     739    3216 
   Size:        739    2477
 
SmaI CCC'GGG

Cuts at:      0     487     960    1798    3216 
   Size:        487     473     838    1418

  Fragments arranged by size: 

                1418     838     487     473
 
SmlI C'TyrA_G

Cuts at:      0      93     367     478    3146    3216 
   Size:         93     274     111    2668      70

  Fragments arranged by size: 

                2668     274     111      93      70
 
StuI AGG'CCT

Cuts at:      0    2044    3216 
   Size:       2044    1172
 
StyI C'CwwG_G

Cuts at:      0    1409    2096    2540    2838    3216 
   Size:       1409     687     444     298     378

  Fragments arranged by size: 

                1409     687     444     378     298
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     399    1005    2016    2366    3216 
   Size:        399     606    1011     350     850

  Fragments arranged by size: 

                1011     850     606     399     350
 
TatI w'GTAC_w

Cuts at:      0    1316    3216 
   Size:       1316    1900
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     444     476    1260    1292    1659    1691    1834    1866 
   Size:        444      32     784      32     367      32     143      32

Cuts at:   1866    1870    1902    2131    2163    3216 
   Size:          4      32     229      32    1053

  Fragments arranged by size: 

                1053     784     444     367     229     143      32      32
                  32      32      32      32       4
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     198     600     843    1297    2637    3216 
   Size:        198     402     243     454    1340     579

  Fragments arranged by size: 

                1340     579     454     402     243     198
 
XhoI C'TCGA_G

Cuts at:      0      93    3146    3216 
   Size:         93    3053      70

  Fragments arranged by size: 

                3053      93      70
 
XmaI C'CCGG_G

Cuts at:      0     485     958    1796    3216 
   Size:        485     473     838    1420

  Fragments arranged by size: 

                1420     838     485     473
 
XmnI GAAnn'nnTTC

Cuts at:      0    1852    3216 
   Size:       1852    1364
 
ZraI GAC'GTC

Cuts at:      0      14    3216 
   Size:         14    3202
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI
    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
    CspCI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    HaeII    Hin4I   HincII     KasI     KpnI     MfeI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NsiI     NspI     PciI    PflMI     PfoI     PpiI    PshAI   PspOMI
    PspXI     PstI    PvuII     SacI    SacII     SalI     SapI    SexAI
     SfcI     SfoI    SgrAI     SmaI     SmlI     StuI     StyI    TaqII
     TatI     TstI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AclI     AfeI    AflII     AgeI     AscI     AseI    AsiSI     BaeI
    BciVI     BlpI     BmtI    BsiWI    BspHI    BsrGI    BstBI     ClaI
     DraI   DraIII     DrdI     EagI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     MluI     NheI     NotI     NruI     PacI
     PasI     PmeI     PmlI    PpuMI     PsiI     PsrI     PvuI    RsrII
    SanDI     SbfI     ScaI     SfiI     SgfI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI  Tth111I     XbaI
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