Restriction map for
pFN21AB3481
(Linear) (Six-base) MAPSORT of: KIBB3481.seq Check: 7705 from: 1 to: 2346
KIBB3481.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 123 2346
Size: 123 2223
AclI AA'CG_TT
Cuts at: 0 846 2346
Size: 846 1500
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 626 2346
Size: 220 406 1720
Fragments arranged by size:
1720 406 220
AfeI AGC'GCT
Cuts at: 0 2335 2346
Size: 2335 11
AflII C'TTAA_G
Cuts at: 0 594 2346
Size: 594 1752
AflIII A'CryG_T
Cuts at: 0 2313 2346
Size: 2313 33
AleI CACnn'nnGTG
Cuts at: 0 154 1369 2346
Size: 154 1215 977
Fragments arranged by size:
1215 977 154
AlwNI CAG_nnn'CTG
Cuts at: 0 1638 2158 2346
Size: 1638 520 188
Fragments arranged by size:
1638 520 188
ApaI G_GGCC'C
Cuts at: 0 689 2346
Size: 689 1657
ApaLI G'TGCA_C
Cuts at: 0 995 1327 2346
Size: 995 332 1019
Fragments arranged by size:
1019 995 332
ApoI r'AATT_y
Cuts at: 0 1440 1549 1618 1772 1782 2194 2346
Size: 1440 109 69 154 10 412 152
Fragments arranged by size:
1440 412 154 152 109 69 10
AvaI C'yCGr_G
Cuts at: 0 87 698 2346
Size: 87 611 1648
Fragments arranged by size:
1648 611 87
BanI G'GyrC_C
Cuts at: 0 148 1532 2233 2301 2346
Size: 148 1384 701 68 45
Fragments arranged by size:
1384 701 148 68 45
BanII G_rGCy'C
Cuts at: 0 626 689 2346
Size: 626 63 1657
Fragments arranged by size:
1657 626 63
BbeI G_GCGC'C
Cuts at: 0 2305 2346
Size: 2305 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 2102 2346
Size: 2102 244
BbvCI CC'TCA_GC
Cuts at: 0 1523 2346
Size: 1523 823
BciVI GTATCCnnnnn_n'
Cuts at: 0 1998 2346
Size: 1998 348
BclI T'GATC_A
Cuts at: 0 1640 2066 2346
Size: 1640 426 280
Fragments arranged by size:
1640 426 280
BglII A'GATC_T
Cuts at: 0 1163 1874 2346
Size: 1163 711 472
Fragments arranged by size:
1163 711 472
BlpI GC'TnA_GC
Cuts at: 0 427 2346
Size: 427 1919
Bme1580I G_kGCm'C
Cuts at: 0 689 999 1331 1537 2346
Size: 689 310 332 206 809
Fragments arranged by size:
809 689 332 310 206
BmrI ACTGGGnnnn_n'
Cuts at: 0 1122 2346
Size: 1122 1224
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2301 2346
Size: 2301 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 254 458 550 823 880 1762 1878
Size: 201 53 204 92 273 57 882 116
Cuts at: 1878 2187 2346
Size: 309 159
Fragments arranged by size:
882 309 273 204 201 159 116 92
57 53
Bpu10I CC'TnA_GC
Cuts at: 0 1457 1523 2346
Size: 1457 66 823
Fragments arranged by size:
1457 823 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1273 2346
Size: 1273 1073
BsaI GGTCTCn'nnnn_
Cuts at: 0 5 2346
Size: 5 2341
BsaHI Gr'CG_yC
Cuts at: 0 83 1899 2302 2346
Size: 83 1816 403 44
Fragments arranged by size:
1816 403 83 44
BsaWI w'CCGG_w
Cuts at: 0 890 2346
Size: 890 1456
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 199 229 2346
Size: 199 30 2117
Fragments arranged by size:
2117 199 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2104 2188 2191 2346
Size: 2104 84 3 155
Fragments arranged by size:
2104 155 84 3
BseYI C'CCAG_C
Cuts at: 0 1089 1236 1389 2188 2305 2346
Size: 1089 147 153 799 117 41
Fragments arranged by size:
1089 799 153 147 117 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1393 2346
Size: 1393 953
BsiHKAI G_wGCw'C
Cuts at: 0 626 999 1331 1364 2346
Size: 626 373 332 33 982
Fragments arranged by size:
982 626 373 332 33
BsmI GAATG_Cn'
Cuts at: 0 592 2346
Size: 592 1754
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1757 2346
Size: 1757 589
Bsp1286I G_dGCh'C
Cuts at: 0 626 689 999 1331 1364 1537 2346
Size: 626 63 310 332 33 173 809
Fragments arranged by size:
809 626 332 310 173 63 33
BspEI T'CCGG_A
Cuts at: 0 890 2346
Size: 890 1456
BspHI T'CATG_A
Cuts at: 0 1310 1650 2346
Size: 1310 340 696
Fragments arranged by size:
1310 696 340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 123 2346
Size: 123 2223
BsrBI CCG'CTC
Cuts at: 0 19 2325 2346
Size: 19 2306 21
Fragments arranged by size:
2306 21 19
BsrFI r'CCGG_y
Cuts at: 0 1169 2346
Size: 1169 1177
BsrGI T'GTAC_A
Cuts at: 0 1598 2270 2316 2346
Size: 1598 672 46 30
Fragments arranged by size:
1598 672 46 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1479 2163 2346
Size: 1479 684 183
Fragments arranged by size:
1479 684 183
BstYI r'GATC_y
Cuts at: 0 708 1163 1874 2206 2346
Size: 708 455 711 332 140
Fragments arranged by size:
711 708 455 332 140
Bsu36I CC'TnA_GG
Cuts at: 0 101 418 2346
Size: 101 317 1928
Fragments arranged by size:
1928 317 101
BtgI C'CryG_G
Cuts at: 0 734 1884 2346
Size: 734 1150 462
Fragments arranged by size:
1150 734 462
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1488 2273 2346
Size: 1488 785 73
Fragments arranged by size:
1488 785 73
BtsI GCAGTG_nn'
Cuts at: 0 684 1123 2346
Size: 684 439 1223
Fragments arranged by size:
1223 684 439
DraI TTT'AAA
Cuts at: 0 1439 2346
Size: 1439 907
EaeI y'GGCC_r
Cuts at: 0 243 2346
Size: 243 2103
EarI CTCTTCn'nnn_
Cuts at: 0 59 71 782 958 1952 2114 2332 2346
Size: 59 12 711 176 994 162 218 14
Fragments arranged by size:
994 711 218 176 162 59 14 12
EcoICRI GAG'CTC
Cuts at: 0 624 2346
Size: 624 1722
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 220 254 458 550 626 823 880
Size: 201 19 34 204 92 76 197 57
Cuts at: 880 1762 1878 2187 2346
Size: 882 116 309 159
Fragments arranged by size:
882 309 204 201 197 159 116 92
76 57 34 19
EcoO109I rG'GnC_Cy
Cuts at: 0 221 570 686 1256 1305 2346
Size: 221 349 116 570 49 1041
Fragments arranged by size:
1041 570 349 221 116 49
EcoRV GAT'ATC
Cuts at: 0 915 2346
Size: 915 1431
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 522 2346
Size: 522 1824
HaeII r_GCGC'y
Cuts at: 0 2229 2305 2337 2346
Size: 2229 76 32 9
Fragments arranged by size:
2229 76 32 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 199 231 2346
Size: 199 32 2115
Fragments arranged by size:
2115 199 32
HincII GTy'rAC
Cuts at: 0 906 2346
Size: 906 1440
KasI G'GCGC_C
Cuts at: 0 2301 2346
Size: 2301 45
MscI TGG'CCA
Cuts at: 0 245 2346
Size: 245 2101
MslI CAynn'nnrTG
Cuts at: 0 154 338 545 733 1369 2346
Size: 154 184 207 188 636 977
Fragments arranged by size:
977 636 207 188 184 154
MspA1I CmG'CkG
Cuts at: 0 198 376 940 1102 2346
Size: 198 178 564 162 1244
Fragments arranged by size:
1244 564 198 178 162
NarI GG'CG_CC
Cuts at: 0 2302 2346
Size: 2302 44
NcoI C'CATG_G
Cuts at: 0 734 1884 2346
Size: 734 1150 462
Fragments arranged by size:
1150 734 462
NspI r_CATG'y
Cuts at: 0 1064 2317 2346
Size: 1064 1253 29
Fragments arranged by size:
1253 1064 29
PciI A'CATG_T
Cuts at: 0 2313 2346
Size: 2313 33
PfoI T'CCnGG_A
Cuts at: 0 40 566 1316 2346
Size: 40 526 750 1030
Fragments arranged by size:
1030 750 526 40
PpuMI rG'GwC_Cy
Cuts at: 0 570 1256 2346
Size: 570 686 1090
Fragments arranged by size:
1090 686 570
PshAI GACnn'nnGTC
Cuts at: 0 439 1408 1749 2346
Size: 439 969 341 597
Fragments arranged by size:
969 597 439 341
PspOMI G'GGCC_C
Cuts at: 0 685 2346
Size: 685 1661
PstI C_TGCA'G
Cuts at: 0 1490 2346
Size: 1490 856
PvuII CAG'CTG
Cuts at: 0 376 940 1102 2346
Size: 376 564 162 1244
Fragments arranged by size:
1244 564 376 162
RsrII CG'GwC_CG
Cuts at: 0 21 2346
Size: 21 2325
SacI G_AGCT'C
Cuts at: 0 626 2346
Size: 626 1720
SapI GCTCTTCn'nnn_
Cuts at: 0 958 2114 2332 2346
Size: 958 1156 218 14
Fragments arranged by size:
1156 958 218 14
SfcI C'TryA_G
Cuts at: 0 180 1486 2346
Size: 180 1306 860
Fragments arranged by size:
1306 860 180
SfoI GGC'GCC
Cuts at: 0 2303 2346
Size: 2303 43
SmaI CCC'GGG
Cuts at: 0 89 2346
Size: 89 2257
SmlI C'TyrA_G
Cuts at: 0 594 1288 2346
Size: 594 694 1058
Fragments arranged by size:
1058 694 594
SphI G_CATG'C
Cuts at: 0 1064 2346
Size: 1064 1282
StyI C'CwwG_G
Cuts at: 0 173 734 1187 1823 1884 2346
Size: 173 561 453 636 61 462
Fragments arranged by size:
636 561 462 453 173 61
SwaI ATTT'AAAT
Cuts at: 0 1439 2346
Size: 1439 907
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 29 219 2346
Size: 29 190 2127
Fragments arranged by size:
2127 190 29
TatI w'GTAC_w
Cuts at: 0 344 475 1598 1961 2270 2316 2346
Size: 344 131 1123 363 309 46 30
Fragments arranged by size:
1123 363 344 309 131 46 30
Tth111I GACn'n_nGTC
Cuts at: 0 801 2346
Size: 801 1545
XmaI C'CCGG_G
Cuts at: 0 87 2346
Size: 87 2259
XmnI GAAnn'nnTTC
Cuts at: 0 110 2346
Size: 110 2236
Enzymes that do cut:
AarI AclI AcuI AfeI AflII AflIII AleI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BsrGI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRV FalI HaeII Hin4I HincII KasI MscI MslI
MspA1I NarI NcoI NspI PciI PfoI PpuMI PshAI
PspOMI PstI PvuII RsrII SacI SapI SfcI SfoI
SmaI SmlI SphI StyI SwaI TaqII TatI Tth111I
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AgeI AhdI AlfI AloI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BfrBI BglI
BmgBI BmtI BsaAI BsaBI BsiEI BsiWI BsrDI BssHII
BssSI BstAPI BstBI BstEII BstZ17I ClaI CspCI DraIII
DrdI EagI EciI EcoNI EcoRI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MmeI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI SacII
SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI TstI XbaI XcmI
XhoI ZraI