(Circular) (Six-base) MAPSORT of: pFN21AB3471.seq Check: 7376 from: 1 to: 6307 pFN21AB3471 6307 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4117 278 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2760 3650 1937 Size: 823 890 4594 Fragments arranged by size: 4594 890 823 Acc65I G'GTAC_C Cuts at: 3628 3628 Size: 6307 AclI AA'CG_TT Cuts at: 1563 4434 4807 1563 Size: 2871 373 3063 Fragments arranged by size: 3063 2871 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2645 3091 3146 4370 5754 6022 1632 Size: 1013 446 55 1224 1384 268 1917 Fragments arranged by size: 1917 1384 1224 1013 446 268 55 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6110 AflIII A'CryG_T Cuts at: 1203 2382 5206 6183 1203 Size: 1179 2824 977 1327 Fragments arranged by size: 2824 1327 1179 977 AgeI A'CCGG_T Cuts at: 5138 5138 Size: 6307 AhdI GACnn_n'nnGTC Cuts at: 2851 5036 2851 Size: 2185 4122 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6307 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2579 1184 Size: 1395 4912 AlwNI CAG_nnn'CTG Cuts at: 2101 2689 2857 3124 4050 5622 2101 Size: 588 168 267 926 1572 2786 Fragments arranged by size: 2786 1572 926 588 267 168 ApaI G_GGCC'C Cuts at: 2274 2710 2274 Size: 436 5871 ApaLI G'TGCA_C Cuts at: 4364 5520 4364 Size: 1156 5151 ApoI r'AATT_y Cuts at: 1493 3616 3842 3994 6103 1493 Size: 2123 226 152 2109 1697 Fragments arranged by size: 2123 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4861 160 Size: 872 3829 1606 Fragments arranged by size: 3829 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 AvaI C'yCGr_G Cuts at: 1870 1943 2254 2273 2468 2744 3064 3632 1870 Size: 73 311 19 195 276 320 568 4545 Fragments arranged by size: 4545 568 320 311 276 195 73 19 BamHI G'GATC_C Cuts at: 2472 3637 4004 2472 Size: 1165 367 4775 Fragments arranged by size: 4775 1165 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2329 2361 3239 3251 3395 Size: 325 217 627 542 32 878 12 144 Cuts at: 3395 3580 3628 5083 618 Size: 185 48 1455 1842 Fragments arranged by size: 1842 1455 878 627 542 325 217 185 144 48 32 12 BanII G_rGCy'C Cuts at: 1391 2055 2274 2280 2710 3626 1391 Size: 664 219 6 430 916 4072 Fragments arranged by size: 4072 916 664 430 219 6 BbeI G_GCGC'C Cuts at: 2365 3243 3399 2365 Size: 878 156 5273 Fragments arranged by size: 5273 878 156 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5336 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2326 2360 4498 4532 1903 Size: 34 389 34 2138 34 3678 Fragments arranged by size: 3678 2138 389 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4199 5409 4199 Size: 1210 5097 BclI T'GATC_A Cuts at: 1548 2613 2895 1548 Size: 1065 282 4960 Fragments arranged by size: 4960 1065 282 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3271 4918 136 Size: 107 122 71 631 605 1599 1647 1525 Fragments arranged by size: 1647 1599 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3015 3045 3354 3453 6302 3015 Size: 30 309 99 2849 3020 Fragments arranged by size: 3020 2849 309 99 30 BlpI GC'TnA_GC Cuts at: 3724 3724 Size: 6307 Bme1580I G_kGCm'C Cuts at: 1790 2274 2422 2710 2908 3276 4368 5524 1790 Size: 484 148 288 198 368 1092 1156 2573 Fragments arranged by size: 2573 1156 1092 484 368 288 198 148 BmgBI CAC'GTC Cuts at: 1318 3313 3472 1318 Size: 1995 159 4153 Fragments arranged by size: 4153 1995 159 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4038 4996 442 Size: 416 53 83 399 24 2621 958 1753 Fragments arranged by size: 2621 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6307 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2158 2158 Size: 6307 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2174 2502 2549 3560 4967 1239 Size: 893 42 328 47 1011 1407 2579 Fragments arranged by size: 2579 1407 1011 893 328 47 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2930 4432 5297 5595 2930 Size: 1502 865 298 3642 Fragments arranged by size: 3642 1502 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4970 882 Size: 617 3471 2219 Fragments arranged by size: 3471 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4098 493 Size: 711 2894 2702 Fragments arranged by size: 2894 2702 711 BsaBI GATnn'nnATC Cuts at: 2264 6301 2264 Size: 4037 2270 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2362 2574 3240 3396 Size: 53 83 186 936 829 212 666 156 Cuts at: 3396 4114 4496 5131 275 Size: 718 382 635 1451 Fragments arranged by size: 1451 936 829 718 666 635 382 212 186 156 83 53 BsaWI w'CCGG_w Cuts at: 4740 5138 5412 5559 5970 4740 Size: 398 274 147 411 5077 Fragments arranged by size: 5077 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2753 2783 3032 3062 1178 Size: 30 165 30 1350 30 249 30 4423 Fragments arranged by size: 4423 1350 249 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 2180 2780 2840 3218 3515 1186 Size: 943 51 600 60 378 297 3978 Fragments arranged by size: 3978 943 600 378 297 60 51 BseYI C'CCAG_C Cuts at: 2013 2421 5510 6006 2013 Size: 408 3089 496 2314 Fragments arranged by size: 3089 2314 496 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5734 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3757 4518 5546 1540 Size: 275 186 1756 761 1028 2301 Fragments arranged by size: 2301 1756 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2055 2121 2190 3042 4368 4453 5524 2055 Size: 66 69 852 1326 85 1071 2838 Fragments arranged by size: 2838 1326 1071 852 85 69 66 BsmI GAATG_Cn' Cuts at: 3818 3911 3818 Size: 93 6214 BsmBI CGTCTCn'nnnn_ Cuts at: 2672 2672 Size: 6307 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2121 2190 2274 2280 2422 2710 Size: 399 265 66 69 84 6 142 288 Cuts at: 2710 2908 3042 3276 3626 4368 4453 5524 1391 Size: 198 134 234 350 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 350 288 265 234 198 142 134 85 84 69 66 6 BspEI T'CCGG_A Cuts at: 5970 5970 Size: 6307 BspHI T'CATG_A Cuts at: 4196 5926 4196 Size: 1730 4577 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3573 3662 844 Size: 2729 89 3489 Fragments arranged by size: 3489 2729 89 BsrBI CCG'CTC Cuts at: 1394 1823 2788 4194 1394 Size: 429 965 1406 3507 Fragments arranged by size: 3507 1406 965 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4802 4976 65 Size: 1183 3554 174 1396 Fragments arranged by size: 3554 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3293 4951 5138 1700 Size: 110 1483 1658 187 2869 Fragments arranged by size: 2869 1658 1483 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6307 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6307 BssSI C'ACGA_G Cuts at: 2132 2192 2345 3548 4367 5379 2132 Size: 60 153 1203 819 1012 3060 Fragments arranged by size: 3060 1203 1012 819 153 60 BstAPI GCAn_nnn'nTGC Cuts at: 794 2101 2692 3580 794 Size: 1307 591 888 3521 Fragments arranged by size: 3521 1307 888 591 BstBI TT'CG_AA Cuts at: 5111 5111 Size: 6307 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6307 BstXI CCAn_nnnn'nTGG Cuts at: 3139 3139 Size: 6307 BstYI r'GATC_y Cuts at: 1972 2472 2643 3015 3045 3354 3453 3637 4004 Size: 500 171 372 30 309 99 184 367 Cuts at: 4004 4389 4406 5858 5944 5956 6302 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 500 385 372 367 346 309 184 171 99 86 30 17 12 Bsu36I CC'TnA_GG Cuts at: 2876 2876 Size: 6307 BtgI C'CryG_G Cuts at: 513 1065 2357 513 Size: 552 1292 4463 Fragments arranged by size: 4463 1292 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2281 3157 487 Size: 683 56 1055 876 3637 Fragments arranged by size: 3637 1055 876 683 56 BtsI GCAGTG_nn' Cuts at: 2855 3823 4617 4637 2855 Size: 968 794 20 4525 Fragments arranged by size: 4525 968 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6307 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6272 DraI TTT'AAA Cuts at: 3611 3611 Size: 6307 DraIII CAC_nnn'GTG Cuts at: 3580 3580 Size: 6307 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5314 809 Size: 507 3998 1802 Fragments arranged by size: 3998 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2913 3162 Size: 54 1425 50 275 18 237 846 249 Cuts at: 3162 3465 3513 3754 4643 6272 8 Size: 303 48 241 889 1629 43 Fragments arranged by size: 1629 1425 889 846 303 275 249 241 237 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3754 1537 Size: 275 1942 4090 Fragments arranged by size: 4090 1942 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2060 2573 2843 4237 1353 Size: 707 513 270 1394 3423 Fragments arranged by size: 3423 1394 707 513 270 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4894 5268 5414 387 Size: 1064 138 3305 374 146 1280 Fragments arranged by size: 3305 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2053 2053 Size: 6307 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2174 2502 2549 2645 3091 3146 Size: 393 500 42 328 47 96 446 55 Cuts at: 3146 3560 4370 4967 5754 6022 1239 Size: 414 810 597 787 268 1524 Fragments arranged by size: 1524 810 787 597 500 446 414 393 328 268 96 55 47 42 EcoNI CCTnn'n_nnAGG Cuts at: 2840 3527 2840 Size: 687 5620 EcoO109I rG'GnC_Cy Cuts at: 2706 2707 2833 2706 Size: 1 126 6180 Fragments arranged by size: 6180 126 1 EcoRI G'AATT_C Cuts at: 3616 3994 3616 Size: 378 5929 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2239 2239 Size: 6307 FspI TGC'GCA Cuts at: 2264 4813 2264 Size: 2549 3758 FspAI rTGC'GCAy Cuts at: 2264 2264 Size: 6307 HaeII r_GCGC'y Cuts at: 1678 2365 3125 3243 3399 5454 1678 Size: 687 760 118 156 2055 2531 Fragments arranged by size: 2531 2055 760 687 156 118 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2223 2255 2588 2605 2620 Size: 32 135 32 651 32 333 17 15 Cuts at: 2620 2637 2837 2869 4948 4980 5022 5054 1373 Size: 17 200 32 2079 32 42 32 2626 Fragments arranged by size: 2626 2079 651 333 200 135 42 32 32 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 3379 3651 3897 669 Size: 988 281 1441 272 246 3079 Fragments arranged by size: 3079 1441 988 281 272 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6307 HpaI GTT'AAC Cuts at: 3897 3897 Size: 6307 KasI G'GCGC_C Cuts at: 2361 3239 3395 2361 Size: 878 156 5273 Fragments arranged by size: 5273 878 156 KpnI G_GTAC'C Cuts at: 3632 3632 Size: 6307 MfeI C'AATT_G Cuts at: 3906 3906 Size: 6307 MluI A'CGCG_T Cuts at: 2382 6183 2382 Size: 3801 2506 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2254 5421 5605 1305 Size: 949 3167 184 2007 Fragments arranged by size: 3167 2007 949 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2915 3164 3515 10 Size: 54 1425 343 1083 249 351 2802 Fragments arranged by size: 2802 1425 1083 351 343 249 54 MslI CAynn'nnrTG Cuts at: 518 1166 4265 4624 4783 518 Size: 648 3099 359 159 2042 Fragments arranged by size: 3099 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2013 2086 2359 2425 2494 2689 3723 4400 Size: 407 73 273 66 69 195 1034 677 Cuts at: 4400 5548 5793 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1034 677 407 273 245 195 73 69 66 NaeI GCC'GGC Cuts at: 3295 3295 Size: 6307 NarI GG'CG_CC Cuts at: 2362 3240 3396 2362 Size: 878 156 5273 Fragments arranged by size: 5273 878 156 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5755 NdeI CA'TA_TG Cuts at: 387 387 Size: 6307 NgoMIV G'CCGG_C Cuts at: 3293 3293 Size: 6307 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6307 NotI GC'GGCC_GC Cuts at: 3754 3754 Size: 6307 NspI r_CATG'y Cuts at: 1132 1229 3160 3665 5210 1132 Size: 97 1931 505 1545 2229 Fragments arranged by size: 2229 1931 1545 505 97 PasI CC'CwG_GG Cuts at: 1348 2722 3053 1348 Size: 1374 331 4602 Fragments arranged by size: 4602 1374 331 PciI A'CATG_T Cuts at: 5206 5206 Size: 6307 PflMI CCAn_nnn'nTGG Cuts at: 1108 2065 2584 1108 Size: 957 519 4831 Fragments arranged by size: 4831 957 519 PfoI T'CCnGG_A Cuts at: 2439 2526 2439 Size: 87 6220 PmeI GTTT'AAAC Cuts at: 3611 3611 Size: 6307 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4376 4408 5889 5921 4376 Size: 32 1481 32 4762 Fragments arranged by size: 4762 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2833 2833 Size: 6307 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6307 PsiI TTA'TAA Cuts at: 3877 3877 Size: 6307 PspOMI G'GGCC_C Cuts at: 2270 2706 2270 Size: 436 5871 PstI C_TGCA'G Cuts at: 830 3096 3659 830 Size: 2266 563 3478 Fragments arranged by size: 3478 2266 563 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6307 PvuII CAG'CTG Cuts at: 2013 2086 2425 2689 2013 Size: 73 339 264 5631 Fragments arranged by size: 5631 339 264 73 SacI G_AGCT'C Cuts at: 2055 2055 Size: 6307 SacII CC_GC'GG Cuts at: 2360 2360 Size: 6307 SalI G'TCGA_C Cuts at: 1936 3649 1936 Size: 1713 4594 SapI GCTCTTCn'nnn_ Cuts at: 2060 2573 2060 Size: 513 5794 SbfI CC_TGCA'GG Cuts at: 3659 3659 Size: 6307 ScaI AGT'ACT Cuts at: 4555 6267 4555 Size: 1712 4595 SexAI A'CCwGG_T Cuts at: 3576 3576 Size: 6307 SfcI C'TryA_G Cuts at: 826 1045 3092 3655 4790 5471 5662 826 Size: 219 2047 563 1135 681 191 1471 Fragments arranged by size: 2047 1471 1135 681 563 219 191 SfoI GGC'GCC Cuts at: 2363 3241 3397 2363 Size: 878 156 5273 Fragments arranged by size: 5273 878 156 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6307 SmaI CCC'GGG Cuts at: 1872 2256 2470 3634 1872 Size: 384 214 1164 4545 Fragments arranged by size: 4545 1164 384 214 SmlI C'TyrA_G Cuts at: 820 1017 1943 2945 4411 5312 5574 820 Size: 197 926 1002 1466 901 262 1553 Fragments arranged by size: 1553 1466 1002 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4098 493 Size: 3605 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6307 SphI G_CATG'C Cuts at: 1132 3665 1132 Size: 2533 3774 SspI AAT'ATT Cuts at: 5 52 4231 5 Size: 47 4179 2081 Fragments arranged by size: 4179 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6307 StyI C'CwwG_G Cuts at: 513 1065 2916 3746 513 Size: 552 1851 830 3074 Fragments arranged by size: 3074 1851 830 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3310 4334 4351 4504 4689 5134 325 Size: 1255 1730 1024 17 153 185 445 1498 Fragments arranged by size: 1730 1498 1255 1024 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2202 4553 6265 Size: 274 80 33 51 1443 225 2351 1712 Cuts at: 6265 96 Size: 138 Fragments arranged by size: 2351 1712 1443 274 225 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3032 3064 6267 6299 3032 Size: 32 3203 32 3040 Fragments arranged by size: 3203 3040 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6307 XbaI T'CTAG_A Cuts at: 3643 3643 Size: 6307 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2323 2437 3050 3412 2323 Size: 114 613 362 5218 Fragments arranged by size: 5218 613 362 114 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6307 XmaI C'CCGG_G Cuts at: 1870 2254 2468 3632 1870 Size: 384 214 1164 4545 Fragments arranged by size: 4545 1164 384 214 XmnI GAAnn'nnTTC Cuts at: 4436 4436 Size: 6307 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4115 276 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoRV FseI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SfiI SrfI SwaI