Restriction map for pFN21AB3419
 (Circular) (Six-base) MAPSORT of: pFN21AB3419.seq  Check: 1176  from: 1  to: 7105



pFN21AB3419  7105 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:26  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4915     278 
   Size:         53      83     186     936    3379    2468

  Fragments arranged by size: 

                3379    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    4448    1937 
   Size:       2511    4594
 
Acc65I G'GTAC_C

Cuts at:   4426    4426 
   Size:       7105
 
AclI AA'CG_TT

Cuts at:   1563    2418    5232    5605    1563 
   Size:        855    2814     373    3063

  Fragments arranged by size: 

                3063    2814     855     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2465    3060    3309    3990    5168    6552    6820    1632 
   Size:        833     595     249     681    1178    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1178     833     681     595     268     249
            
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6908
 
AflIII A'CryG_T

Cuts at:   1203    2493    3586    6004    6981    1203 
   Size:       1290    1093    2418     977    1327

  Fragments arranged by size: 

                2418    1327    1290    1093     977
 
AgeI A'CCGG_T

Cuts at:   5936    5936 
   Size:       7105
 
AhdI GACnn_n'nnGTC

Cuts at:   2407    5834    2407 
   Size:       3427    3678
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       7105
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       7105
 
AlwNI CAG_nnn'CTG

Cuts at:   3353    4848    6420    3353 
   Size:       1495    1572    4038

  Fragments arranged by size: 

                4038    1572    1495
 
ApaLI G'TGCA_C

Cuts at:   3538    5162    6318    3538 
   Size:       1624    1156    4325

  Fragments arranged by size: 

                4325    1624    1156
 
ApoI r'AATT_y

Cuts at:   1493    2039    3241    4414    4640    4792    6901    1493 
   Size:        546    1202    1173     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1202    1173     546     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    5659     160 
   Size:        872    4627    1606

  Fragments arranged by size: 

                4627    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7105
 
AvaI C'yCGr_G

Cuts at:   1870    1943    4430    1870 
   Size:         73    2487    4545

  Fragments arranged by size: 

                4545    2487      73
 
BamHI G'GATC_C

Cuts at:   4435    4802    4435 
   Size:        367    6738
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2018    2462    3862    4426    5881 
   Size:        325     217     627     231     444    1400     564    1455

Cuts at:   5881     618 
   Size:       1842

  Fragments arranged by size: 

                1842    1455    1400     627     564     444     325     231
                 217
 
BanII G_rGCy'C

Cuts at:   1391    4424    1391 
   Size:       3033    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3033    3162     928 
   Size:        971    1134     129    4871

  Fragments arranged by size: 

                4871    1134     971     129
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3689    3723    5296    5330    1903 
   Size:         34    1752      34    1573      34    3678

  Fragments arranged by size: 

                3678    1752    1573      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4997    6207    4997 
   Size:       1210    5895
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       7105
 
BfrBI ATG'CAT

Cuts at:   2359    2359 
   Size:       7105
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4339    5716     136 
   Size:        107     122      71     631     605    2667    1377    1525

  Fragments arranged by size: 

                2667    1525    1377     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   2448    7100    2448 
   Size:       4652    2453
 
BlpI GC'TnA_GC

Cuts at:   4522    4522 
   Size:       7105
 
Bme1580I G_kGCm'C

Cuts at:   1790    3542    3867    5166    6322    1790 
   Size:       1752     325    1299    1156    2573

  Fragments arranged by size: 

                2573    1752    1299    1156     325
 
BmgBI CAC'GTC

Cuts at:   1318    4052    1318 
   Size:       2734    4371
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4836    5794     442 
   Size:        416      53      83     399      24    3419     958    1753

  Fragments arranged by size: 

                3419    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       7105
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4279    4279 
   Size:       7105
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2511    2804    3167    5765    1239 
   Size:       1272     293     363    2598    2579

  Fragments arranged by size: 

                2598    2579    1272     363     293
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2390    2897    3688    4249    5230    6095    6393    2390 
   Size:        507     791     561     981     865     298    3102

  Fragments arranged by size: 

                3102     981     865     791     561     507     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2394    5768     882 
   Size:        617     895    3374    2219

  Fragments arranged by size: 

                3374    2219     895     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4896     493 
   Size:        711    3692    2702

  Fragments arranged by size: 

                3692    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   2316    7099    2316 
   Size:       4783    2322
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4912    5294    5929     275 
   Size:         53      83     186     936    3379     382     635    1451

  Fragments arranged by size: 

                3379    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4323    5538    5936    6210    6357    6768    4323 
   Size:       1215     398     274     147     411    4660

  Fragments arranged by size: 

                4660    1215     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2777    2807    2840    2870    1178 
   Size:         30     165      30    1374      30      33      30    5413

  Fragments arranged by size: 

                5413    1374     165      33      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2135    2318    1186 
   Size:        949     183    5973

  Fragments arranged by size: 

                5973     949     183
 
BseYI C'CCAG_C

Cuts at:   6308    6804    6308 
   Size:        496    6609
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    6532
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    4555    5316    6344    1540 
   Size:        275     186    2554     761    1028    2301

  Fragments arranged by size: 

                2554    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3542    5166    5251    6322    3542 
   Size:       1624      85    1071    4325

  Fragments arranged by size: 

                4325    1624    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2361    4108    4616    4709    2361 
   Size:       1747     508      93    4757

  Fragments arranged by size: 

                4757    1747     508      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2016    3844    3918    2016 
   Size:       1828      74    5203

  Fragments arranged by size: 

                5203    1828      74
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    3542    3867    4424    5166    5251    6322    1391 
   Size:        399    1752     325     557     742      85    1071    2174

  Fragments arranged by size: 

                2174    1752    1071     742     557     399     325      85
            
 
BspEI T'CCGG_A

Cuts at:   6768    6768 
   Size:       7105
 
BspHI T'CATG_A

Cuts at:   4994    6724    4994 
   Size:       1730    5375
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3684    4460     844 
   Size:       2840     776    3489

  Fragments arranged by size: 

                3489    2840     776
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4992    1394 
   Size:        429    3169    3507

  Fragments arranged by size: 

                3507    3169     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    5600    5774      65 
   Size:       1183    4352     174    1396

  Fragments arranged by size: 

                4352    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5749    5936    1700 
   Size:        110    3939     187    2869

  Fragments arranged by size: 

                3939    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       7105
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       7105
 
BssSI C'ACGA_G

Cuts at:   2803    5165    6177    2803 
   Size:       2362    1012    3731

  Fragments arranged by size: 

                3731    2362    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       7105
 
BstBI TT'CG_AA

Cuts at:   5909    5909 
   Size:       7105
 
BstEII G'GTnAC_C

Cuts at:   1184    3798    1184 
   Size:       2614    4491
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2210    2210 
   Size:       7105
 
BstYI r'GATC_y

Cuts at:   1972    2448    4435    4802    5187    5204    6656    6742    6754 
   Size:        476    1987     367     385      17    1452      86      12

Cuts at:   6754    7100    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1987    1977    1452     476     385     367     346      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   3621    3621 
   Size:       7105
 
BtgI C'CryG_G

Cuts at:    513    1065    2516    4387     513 
   Size:        552    1451    1871    3231

  Fragments arranged by size: 

                3231    1871    1451     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    6366

  Fragments arranged by size: 

                6366     683      56
 
BtsI GCAGTG_nn'

Cuts at:   4621    5415    5435    4621 
   Size:        794      20    6291

  Fragments arranged by size: 

                6291     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       7105
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    7070
 
DraI TTT'AAA

Cuts at:   2623    2949    3109    3468    4409    2623 
   Size:        326     160     359     941    5319

  Fragments arranged by size: 

                5319     941     359     326     160
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2977    4289    6112     809 
   Size:        507    1661    1312    1823    1802

  Fragments arranged by size: 

                1823    1802    1661    1312     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2753    3823    4552 
   Size:         54    1425      50     275      18     923    1070     729

Cuts at:   4552    5441    7070       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425    1070     923     889     729     275      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    4552    1537 
   Size:        275    2740    4090

  Fragments arranged by size: 

                4090    2740     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3079    3952    4009    4272    5035    1353 
   Size:       1726     873      57     263     763    3423

  Fragments arranged by size: 

                3423    1726     873     763     263      57
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2861    5692    6066    6212     387 
   Size:       1064     138    1272    2831     374     146    1280

  Fragments arranged by size: 

                2831    1280    1272    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2465    2511    2804    3060    3167    3309    3990 
   Size:        393     833      46     293     256     107     142     681

Cuts at:   3990    5168    5765    6552    6820    1239 
   Size:       1178     597     787     268    1524

  Fragments arranged by size: 

                1524    1178     833     787     681     597     393     293
                 268     256     142     107      46
 
EcoNI CCTnn'n_nnAGG

Cuts at:   4122    4122 
   Size:       7105
 
EcoO109I rG'GnC_Cy

Cuts at:   2614    4210    2614 
   Size:       1596    5509
 
EcoRI G'AATT_C

Cuts at:   4414    4792    4414 
   Size:        378    6727
 
EcoRV GAT'ATC

Cuts at:   2112    2112 
   Size:       7105
 
FspI TGC'GCA

Cuts at:   5611    5611 
   Size:       7105
 
HaeII r_GCGC'y

Cuts at:   1678    6252    1678 
   Size:       4574    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2080    2112    2393    2425    2777 
   Size:         32     135      32     508      32     281      32     352

Cuts at:   2777    2809    5746    5778    5820    5852    1373 
   Size:         32    2937      32      42      32    2626

  Fragments arranged by size: 

                2937    2626     508     352     281     135      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    4449    4695     669 
   Size:        988     281    2511     246    3079

  Fragments arranged by size: 

                3079    2511     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    3100     748 
   Size:       2352    4753
 
HpaI GTT'AAC

Cuts at:   4695    4695 
   Size:       7105
 
KpnI G_GTAC'C

Cuts at:   4430    4430 
   Size:       7105
 
MfeI C'AATT_G

Cuts at:   4704    4704 
   Size:       7105
 
MluI A'CGCG_T

Cuts at:   6981    6981 
   Size:       7105
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2485    3006    3667    3723    3780    6219    6403    1305 
   Size:       1180     521     661      56      57    2439     184    2007

  Fragments arranged by size: 

                2439    2007    1180     661     521     184      57      56
            
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    3825      10 
   Size:         54    1425     343    1993    3290

  Fragments arranged by size: 

                3290    1993    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3819    5063    5422    5581     518 
   Size:        648    2653    1244     359     159    2042

  Fragments arranged by size: 

                2653    2042    1244     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2992    4521    5198    6346    6591    1606 
   Size:       1386    1529     677    1148     245    2120

  Fragments arranged by size: 

                2120    1529    1386    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2516     513 
   Size:        552    1451    5102

  Fragments arranged by size: 

                5102    1451     552
 
NdeI CA'TA_TG

Cuts at:    387    2370    3550     387 
   Size:       1983    1180    3942

  Fragments arranged by size: 

                3942    1983    1180
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       7105
 
NotI GC'GGCC_GC

Cuts at:   4552    4552 
   Size:       7105
 
NsiI A_TGCA'T

Cuts at:   2361    2361 
   Size:       7105
 
NspI r_CATG'y

Cuts at:   1132    1229    2497    3590    4463    6008    1132 
   Size:         97    1268    1093     873    1545    2229

  Fragments arranged by size: 

                2229    1545    1268    1093     873      97
 
PasI CC'CwG_GG

Cuts at:   1348    4206    1348 
   Size:       2858    4247
 
PciI A'CATG_T

Cuts at:   2493    3586    6004    2493 
   Size:       1093    2418    3594

  Fragments arranged by size: 

                3594    2418    1093
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    3353    1108 
   Size:       2245    4860
 
PfoI T'CCnGG_A

Cuts at:   2781    2781 
   Size:       7105
 
PmeI GTTT'AAAC

Cuts at:   4409    4409 
   Size:       7105
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3604    3636    5174    5206    6687    6719    3604 
   Size:         32    1538      32    1481      32    3990

  Fragments arranged by size: 

                3990    1538    1481      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2614    4210    2614 
   Size:       1596    5509
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       7105
 
PsiI TTA'TAA

Cuts at:   4675    4675 
   Size:       7105
 
PstI C_TGCA'G

Cuts at:    830    4457     830 
   Size:       3627    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       7105
 
SalI G'TCGA_C

Cuts at:   1936    4447    1936 
   Size:       2511    4594
 
SbfI CC_TGCA'GG

Cuts at:   4457    4457 
   Size:       7105
 
ScaI AGT'ACT

Cuts at:   2442    5353    7065    2442 
   Size:       2911    1712    2482

  Fragments arranged by size: 

                2911    2482    1712
 
SfcI C'TryA_G

Cuts at:    826    1045    4147    4453    5588    6269    6460     826 
   Size:        219    3102     306    1135     681     191    1471

  Fragments arranged by size: 

                3102    1471    1135     681     306     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7105
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       7105
 
SmaI CCC'GGG

Cuts at:   1872    4432    1872 
   Size:       2560    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2405    2876    3703    4264    5209    6110 
   Size:        197     926     462     471     827     561     945     901

Cuts at:   6110    6372     820 
   Size:        262    1553

  Fragments arranged by size: 

                1553     945     926     901     827     561     471     462
                 262     197
 
SnaBI TAC'GTA

Cuts at:    493    4896     493 
   Size:       4403    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       7105
 
SphI G_CATG'C

Cuts at:   1132    4463    1132 
   Size:       3331    3774
 
SspI AAT'ATT

Cuts at:      5      52    5029       5 
   Size:         47    4977    2081

  Fragments arranged by size: 

                4977    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       7105
 
StyI C'CwwG_G

Cuts at:    513    1065    2499    2516    3183    4544     513 
   Size:        552    1434      17     667    1361    3074

  Fragments arranged by size: 

                3074    1434    1361     667     552      17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    5132    5149    5302    5487    5932     325 
   Size:       1255    3552      17     153     185     445    1498

  Fragments arranged by size: 

                3552    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2440    5351    7063 
   Size:        274      80      33      51    1443     463    2911    1712

Cuts at:   7063      96 
   Size:        138

  Fragments arranged by size: 

                2911    1712    1443     463     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2794    2826    2840    2872    3356    3388    7065    7097    2794 
   Size:         32      14      32     484      32    3677      32    2802

  Fragments arranged by size: 

                3677    2802     484      32      32      32      32      14
            
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       7105
 
XbaI T'CTAG_A

Cuts at:   4441    4441 
   Size:       7105
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       7105
 
XmaI C'CCGG_G

Cuts at:   1870    4430    1870 
   Size:       2560    4545
 
XmnI GAAnn'nnTTC

Cuts at:   5234    5234 
   Size:       7105
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4913     276 
   Size:         53      83     186     936    3379    2468

  Fragments arranged by size: 

                3379    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI
     NotI     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI     SalI     SbfI
     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI   Bpu10I    BsiWI  BstZ17I   DraIII  EcoICRI     FalI     FseI
    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     PacI     PmlI
   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI    SacII    SanDI
     SapI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com