(Circular) (Six-base) MAPSORT of: pFN21AB3419.seq Check: 1176 from: 1 to: 7105 pFN21AB3419 7105 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4915 278 Size: 53 83 186 936 3379 2468 Fragments arranged by size: 3379 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4448 1937 Size: 2511 4594 Acc65I G'GTAC_C Cuts at: 4426 4426 Size: 7105 AclI AA'CG_TT Cuts at: 1563 2418 5232 5605 1563 Size: 855 2814 373 3063 Fragments arranged by size: 3063 2814 855 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2465 3060 3309 3990 5168 6552 6820 1632 Size: 833 595 249 681 1178 1384 268 1917 Fragments arranged by size: 1917 1384 1178 833 681 595 268 249 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6908 AflIII A'CryG_T Cuts at: 1203 2493 3586 6004 6981 1203 Size: 1290 1093 2418 977 1327 Fragments arranged by size: 2418 1327 1290 1093 977 AgeI A'CCGG_T Cuts at: 5936 5936 Size: 7105 AhdI GACnn_n'nnGTC Cuts at: 2407 5834 2407 Size: 3427 3678 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7105 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7105 AlwNI CAG_nnn'CTG Cuts at: 3353 4848 6420 3353 Size: 1495 1572 4038 Fragments arranged by size: 4038 1572 1495 ApaLI G'TGCA_C Cuts at: 3538 5162 6318 3538 Size: 1624 1156 4325 Fragments arranged by size: 4325 1624 1156 ApoI r'AATT_y Cuts at: 1493 2039 3241 4414 4640 4792 6901 1493 Size: 546 1202 1173 226 152 2109 1697 Fragments arranged by size: 2109 1697 1202 1173 546 226 152 AseI AT'TA_AT Cuts at: 160 1032 5659 160 Size: 872 4627 1606 Fragments arranged by size: 4627 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7105 AvaI C'yCGr_G Cuts at: 1870 1943 4430 1870 Size: 73 2487 4545 Fragments arranged by size: 4545 2487 73 BamHI G'GATC_C Cuts at: 4435 4802 4435 Size: 367 6738 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2018 2462 3862 4426 5881 Size: 325 217 627 231 444 1400 564 1455 Cuts at: 5881 618 Size: 1842 Fragments arranged by size: 1842 1455 1400 627 564 444 325 231 217 BanII G_rGCy'C Cuts at: 1391 4424 1391 Size: 3033 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3033 3162 928 Size: 971 1134 129 4871 Fragments arranged by size: 4871 1134 971 129 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3689 3723 5296 5330 1903 Size: 34 1752 34 1573 34 3678 Fragments arranged by size: 3678 1752 1573 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4997 6207 4997 Size: 1210 5895 BclI T'GATC_A Cuts at: 1548 1548 Size: 7105 BfrBI ATG'CAT Cuts at: 2359 2359 Size: 7105 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4339 5716 136 Size: 107 122 71 631 605 2667 1377 1525 Fragments arranged by size: 2667 1525 1377 631 605 122 107 71 BglII A'GATC_T Cuts at: 2448 7100 2448 Size: 4652 2453 BlpI GC'TnA_GC Cuts at: 4522 4522 Size: 7105 Bme1580I G_kGCm'C Cuts at: 1790 3542 3867 5166 6322 1790 Size: 1752 325 1299 1156 2573 Fragments arranged by size: 2573 1752 1299 1156 325 BmgBI CAC'GTC Cuts at: 1318 4052 1318 Size: 2734 4371 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4836 5794 442 Size: 416 53 83 399 24 3419 958 1753 Fragments arranged by size: 3419 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7105 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4279 4279 Size: 7105 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2511 2804 3167 5765 1239 Size: 1272 293 363 2598 2579 Fragments arranged by size: 2598 2579 1272 363 293 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2390 2897 3688 4249 5230 6095 6393 2390 Size: 507 791 561 981 865 298 3102 Fragments arranged by size: 3102 981 865 791 561 507 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2394 5768 882 Size: 617 895 3374 2219 Fragments arranged by size: 3374 2219 895 617 BsaAI yAC'GTr Cuts at: 493 1204 4896 493 Size: 711 3692 2702 Fragments arranged by size: 3692 2702 711 BsaBI GATnn'nnATC Cuts at: 2316 7099 2316 Size: 4783 2322 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4912 5294 5929 275 Size: 53 83 186 936 3379 382 635 1451 Fragments arranged by size: 3379 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4323 5538 5936 6210 6357 6768 4323 Size: 1215 398 274 147 411 4660 Fragments arranged by size: 4660 1215 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2777 2807 2840 2870 1178 Size: 30 165 30 1374 30 33 30 5413 Fragments arranged by size: 5413 1374 165 33 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2318 1186 Size: 949 183 5973 Fragments arranged by size: 5973 949 183 BseYI C'CCAG_C Cuts at: 6308 6804 6308 Size: 496 6609 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6532 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4555 5316 6344 1540 Size: 275 186 2554 761 1028 2301 Fragments arranged by size: 2554 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3542 5166 5251 6322 3542 Size: 1624 85 1071 4325 Fragments arranged by size: 4325 1624 1071 85 BsmI GAATG_Cn' Cuts at: 2361 4108 4616 4709 2361 Size: 1747 508 93 4757 Fragments arranged by size: 4757 1747 508 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2016 3844 3918 2016 Size: 1828 74 5203 Fragments arranged by size: 5203 1828 74 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3542 3867 4424 5166 5251 6322 1391 Size: 399 1752 325 557 742 85 1071 2174 Fragments arranged by size: 2174 1752 1071 742 557 399 325 85 BspEI T'CCGG_A Cuts at: 6768 6768 Size: 7105 BspHI T'CATG_A Cuts at: 4994 6724 4994 Size: 1730 5375 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3684 4460 844 Size: 2840 776 3489 Fragments arranged by size: 3489 2840 776 BsrBI CCG'CTC Cuts at: 1394 1823 4992 1394 Size: 429 3169 3507 Fragments arranged by size: 3507 3169 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5600 5774 65 Size: 1183 4352 174 1396 Fragments arranged by size: 4352 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5749 5936 1700 Size: 110 3939 187 2869 Fragments arranged by size: 3939 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7105 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7105 BssSI C'ACGA_G Cuts at: 2803 5165 6177 2803 Size: 2362 1012 3731 Fragments arranged by size: 3731 2362 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7105 BstBI TT'CG_AA Cuts at: 5909 5909 Size: 7105 BstEII G'GTnAC_C Cuts at: 1184 3798 1184 Size: 2614 4491 BstXI CCAn_nnnn'nTGG Cuts at: 2210 2210 Size: 7105 BstYI r'GATC_y Cuts at: 1972 2448 4435 4802 5187 5204 6656 6742 6754 Size: 476 1987 367 385 17 1452 86 12 Cuts at: 6754 7100 1972 Size: 346 1977 Fragments arranged by size: 1987 1977 1452 476 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3621 3621 Size: 7105 BtgI C'CryG_G Cuts at: 513 1065 2516 4387 513 Size: 552 1451 1871 3231 Fragments arranged by size: 3231 1871 1451 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6366 Fragments arranged by size: 6366 683 56 BtsI GCAGTG_nn' Cuts at: 4621 5415 5435 4621 Size: 794 20 6291 Fragments arranged by size: 6291 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7105 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7070 DraI TTT'AAA Cuts at: 2623 2949 3109 3468 4409 2623 Size: 326 160 359 941 5319 Fragments arranged by size: 5319 941 359 326 160 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2977 4289 6112 809 Size: 507 1661 1312 1823 1802 Fragments arranged by size: 1823 1802 1661 1312 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2753 3823 4552 Size: 54 1425 50 275 18 923 1070 729 Cuts at: 4552 5441 7070 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1070 923 889 729 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4552 1537 Size: 275 2740 4090 Fragments arranged by size: 4090 2740 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3079 3952 4009 4272 5035 1353 Size: 1726 873 57 263 763 3423 Fragments arranged by size: 3423 1726 873 763 263 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2861 5692 6066 6212 387 Size: 1064 138 1272 2831 374 146 1280 Fragments arranged by size: 2831 1280 1272 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2465 2511 2804 3060 3167 3309 3990 Size: 393 833 46 293 256 107 142 681 Cuts at: 3990 5168 5765 6552 6820 1239 Size: 1178 597 787 268 1524 Fragments arranged by size: 1524 1178 833 787 681 597 393 293 268 256 142 107 46 EcoNI CCTnn'n_nnAGG Cuts at: 4122 4122 Size: 7105 EcoO109I rG'GnC_Cy Cuts at: 2614 4210 2614 Size: 1596 5509 EcoRI G'AATT_C Cuts at: 4414 4792 4414 Size: 378 6727 EcoRV GAT'ATC Cuts at: 2112 2112 Size: 7105 FspI TGC'GCA Cuts at: 5611 5611 Size: 7105 HaeII r_GCGC'y Cuts at: 1678 6252 1678 Size: 4574 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2080 2112 2393 2425 2777 Size: 32 135 32 508 32 281 32 352 Cuts at: 2777 2809 5746 5778 5820 5852 1373 Size: 32 2937 32 42 32 2626 Fragments arranged by size: 2937 2626 508 352 281 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4449 4695 669 Size: 988 281 2511 246 3079 Fragments arranged by size: 3079 2511 988 281 246 HindIII A'AGCT_T Cuts at: 748 3100 748 Size: 2352 4753 HpaI GTT'AAC Cuts at: 4695 4695 Size: 7105 KpnI G_GTAC'C Cuts at: 4430 4430 Size: 7105 MfeI C'AATT_G Cuts at: 4704 4704 Size: 7105 MluI A'CGCG_T Cuts at: 6981 6981 Size: 7105 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2485 3006 3667 3723 3780 6219 6403 1305 Size: 1180 521 661 56 57 2439 184 2007 Fragments arranged by size: 2439 2007 1180 661 521 184 57 56 MscI TGG'CCA Cuts at: 10 64 1489 1832 3825 10 Size: 54 1425 343 1993 3290 Fragments arranged by size: 3290 1993 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3819 5063 5422 5581 518 Size: 648 2653 1244 359 159 2042 Fragments arranged by size: 2653 2042 1244 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2992 4521 5198 6346 6591 1606 Size: 1386 1529 677 1148 245 2120 Fragments arranged by size: 2120 1529 1386 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2516 513 Size: 552 1451 5102 Fragments arranged by size: 5102 1451 552 NdeI CA'TA_TG Cuts at: 387 2370 3550 387 Size: 1983 1180 3942 Fragments arranged by size: 3942 1983 1180 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7105 NotI GC'GGCC_GC Cuts at: 4552 4552 Size: 7105 NsiI A_TGCA'T Cuts at: 2361 2361 Size: 7105 NspI r_CATG'y Cuts at: 1132 1229 2497 3590 4463 6008 1132 Size: 97 1268 1093 873 1545 2229 Fragments arranged by size: 2229 1545 1268 1093 873 97 PasI CC'CwG_GG Cuts at: 1348 4206 1348 Size: 2858 4247 PciI A'CATG_T Cuts at: 2493 3586 6004 2493 Size: 1093 2418 3594 Fragments arranged by size: 3594 2418 1093 PflMI CCAn_nnn'nTGG Cuts at: 1108 3353 1108 Size: 2245 4860 PfoI T'CCnGG_A Cuts at: 2781 2781 Size: 7105 PmeI GTTT'AAAC Cuts at: 4409 4409 Size: 7105 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3604 3636 5174 5206 6687 6719 3604 Size: 32 1538 32 1481 32 3990 Fragments arranged by size: 3990 1538 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2614 4210 2614 Size: 1596 5509 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7105 PsiI TTA'TAA Cuts at: 4675 4675 Size: 7105 PstI C_TGCA'G Cuts at: 830 4457 830 Size: 3627 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7105 SalI G'TCGA_C Cuts at: 1936 4447 1936 Size: 2511 4594 SbfI CC_TGCA'GG Cuts at: 4457 4457 Size: 7105 ScaI AGT'ACT Cuts at: 2442 5353 7065 2442 Size: 2911 1712 2482 Fragments arranged by size: 2911 2482 1712 SfcI C'TryA_G Cuts at: 826 1045 4147 4453 5588 6269 6460 826 Size: 219 3102 306 1135 681 191 1471 Fragments arranged by size: 3102 1471 1135 681 306 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7105 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7105 SmaI CCC'GGG Cuts at: 1872 4432 1872 Size: 2560 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2405 2876 3703 4264 5209 6110 Size: 197 926 462 471 827 561 945 901 Cuts at: 6110 6372 820 Size: 262 1553 Fragments arranged by size: 1553 945 926 901 827 561 471 462 262 197 SnaBI TAC'GTA Cuts at: 493 4896 493 Size: 4403 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7105 SphI G_CATG'C Cuts at: 1132 4463 1132 Size: 3331 3774 SspI AAT'ATT Cuts at: 5 52 5029 5 Size: 47 4977 2081 Fragments arranged by size: 4977 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7105 StyI C'CwwG_G Cuts at: 513 1065 2499 2516 3183 4544 513 Size: 552 1434 17 667 1361 3074 Fragments arranged by size: 3074 1434 1361 667 552 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5132 5149 5302 5487 5932 325 Size: 1255 3552 17 153 185 445 1498 Fragments arranged by size: 3552 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2440 5351 7063 Size: 274 80 33 51 1443 463 2911 1712 Cuts at: 7063 96 Size: 138 Fragments arranged by size: 2911 1712 1443 463 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2794 2826 2840 2872 3356 3388 7065 7097 2794 Size: 32 14 32 484 32 3677 32 2802 Fragments arranged by size: 3677 2802 484 32 32 32 32 14 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7105 XbaI T'CTAG_A Cuts at: 4441 4441 Size: 7105 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7105 XmaI C'CCGG_G Cuts at: 1870 4430 1870 Size: 2560 4545 XmnI GAAnn'nnTTC Cuts at: 5234 5234 Size: 7105 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4913 276 Size: 53 83 186 936 3379 2468 Fragments arranged by size: 3379 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI