(Circular) (Six-base) MAPSORT of: pFN21AB3414.seq Check: 6872 from: 1 to: 7333 pFN21AB3414 7333 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2653 3083 2653 Size: 430 6903 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5143 278 Size: 53 83 186 936 3607 2468 Fragments arranged by size: 3607 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2179 2450 3345 4676 1937 Size: 242 271 895 1331 4594 Fragments arranged by size: 4594 1331 895 271 242 Acc65I G'GTAC_C Cuts at: 3147 4654 3147 Size: 1507 5826 AclI AA'CG_TT Cuts at: 1563 5460 5833 1563 Size: 3897 373 3063 Fragments arranged by size: 3897 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2880 3345 3459 3674 3728 3752 4205 4569 Size: 1248 465 114 215 54 24 453 364 Cuts at: 4569 4640 5396 6780 7048 1632 Size: 71 756 1384 268 1917 Fragments arranged by size: 1917 1384 1248 756 465 453 364 268 215 114 71 54 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7136 AflIII A'CryG_T Cuts at: 1203 3100 6232 7209 1203 Size: 1897 3132 977 1327 Fragments arranged by size: 3132 1897 1327 977 AgeI A'CCGG_T Cuts at: 6164 6164 Size: 7333 AhdI GACnn_n'nnGTC Cuts at: 2273 3745 6062 2273 Size: 1472 2317 3544 Fragments arranged by size: 3544 2317 1472 AleI CACnn'nnGTG Cuts at: 1166 3908 1166 Size: 2742 4591 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3070 1184 Size: 1886 5447 AlwNI CAG_nnn'CTG Cuts at: 2725 2968 3370 4144 5076 6648 2725 Size: 243 402 774 932 1572 3410 Fragments arranged by size: 3410 1572 932 774 402 243 ApaLI G'TGCA_C Cuts at: 5390 6546 5390 Size: 1156 6177 ApoI r'AATT_y Cuts at: 1493 2846 3306 3929 4099 4388 4642 4868 5020 Size: 1353 460 623 170 289 254 226 152 Cuts at: 5020 7129 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1353 623 460 289 254 226 170 152 AseI AT'TA_AT Cuts at: 160 1032 3251 5887 160 Size: 872 2219 2636 1606 Fragments arranged by size: 2636 2219 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7333 AvaI C'yCGr_G Cuts at: 1870 1943 2024 4658 1870 Size: 73 81 2634 4545 Fragments arranged by size: 4545 2634 81 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2685 2718 2948 2981 2685 Size: 33 230 33 7037 Fragments arranged by size: 7037 230 33 33 BamHI G'GATC_C Cuts at: 4663 5030 4663 Size: 367 6966 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3147 4654 6109 618 Size: 325 217 627 1360 1507 1455 1842 Fragments arranged by size: 1842 1507 1455 1360 627 325 217 BanII G_rGCy'C Cuts at: 1391 3201 4652 1391 Size: 1810 1451 4072 Fragments arranged by size: 4072 1810 1451 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2036 4571 928 Size: 971 137 2535 3690 Fragments arranged by size: 3690 2535 971 137 BbvCI CC'TCA_GC Cuts at: 2397 2397 Size: 7333 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5524 5558 1903 Size: 34 3587 34 3678 Fragments arranged by size: 3678 3587 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5225 6435 5225 Size: 1210 6123 BclI T'GATC_A Cuts at: 1548 2013 3472 1548 Size: 465 1459 5409 Fragments arranged by size: 5409 1459 465 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3893 5944 136 Size: 107 122 71 631 605 2221 2051 1525 Fragments arranged by size: 2221 2051 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3810 7328 3810 Size: 3518 3815 BlpI GC'TnA_GC Cuts at: 4750 4750 Size: 7333 Bme1580I G_kGCm'C Cuts at: 1790 4045 5394 6550 1790 Size: 2255 1349 1156 2573 Fragments arranged by size: 2573 2255 1349 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7333 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2363 3195 3258 Size: 416 53 83 399 24 946 832 63 Cuts at: 3258 5064 6022 442 Size: 1806 958 1753 Fragments arranged by size: 1806 1753 958 946 832 416 399 83 63 53 24 BmtI G_CTAG'C Cuts at: 1056 2582 1056 Size: 1526 5807 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2072 2099 3412 4440 5993 1239 Size: 833 27 1313 1028 1553 2579 Fragments arranged by size: 2579 1553 1313 1028 833 27 Bpu10I CC'TnA_GC Cuts at: 2397 2925 4286 2397 Size: 528 1361 5444 Fragments arranged by size: 5444 1361 528 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3920 5458 6323 6621 3920 Size: 1538 865 298 4632 Fragments arranged by size: 4632 1538 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3386 5996 882 Size: 617 1887 2610 2219 Fragments arranged by size: 2610 2219 1887 617 BsaAI yAC'GTr Cuts at: 493 1204 5124 493 Size: 711 3920 2702 Fragments arranged by size: 3920 2702 711 BsaBI GATnn'nnATC Cuts at: 3633 4428 7327 3633 Size: 795 2899 3639 Fragments arranged by size: 3639 2899 795 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5140 5522 6157 275 Size: 53 83 186 936 3607 382 635 1451 Fragments arranged by size: 3607 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5766 6164 6438 6585 6996 5766 Size: 398 274 147 411 6103 Fragments arranged by size: 6103 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2023 2053 3090 3120 1178 Size: 30 165 30 620 30 1037 30 5391 Fragments arranged by size: 5391 1037 620 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3592 4194 1186 Size: 2406 602 4325 Fragments arranged by size: 4325 2406 602 BseYI C'CCAG_C Cuts at: 3417 3860 6536 7032 3417 Size: 443 2676 496 3718 Fragments arranged by size: 3718 2676 496 443 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2379 1163 Size: 573 643 6117 Fragments arranged by size: 6117 643 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2047 2091 4783 5544 6572 1540 Size: 275 186 46 44 2692 761 1028 2301 Fragments arranged by size: 2692 2301 1028 761 275 186 46 44 BsiHKAI G_wGCw'C Cuts at: 2439 2931 2959 3716 5394 5479 6550 2439 Size: 492 28 757 1678 85 1071 3222 Fragments arranged by size: 3222 1678 1071 757 492 85 28 BsmI GAATG_Cn' Cuts at: 2596 4844 4937 2596 Size: 2248 93 4992 Fragments arranged by size: 4992 2248 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2439 2931 2959 3201 3716 4045 4652 Size: 399 649 492 28 242 515 329 607 Cuts at: 4652 5394 5479 6550 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 649 607 515 492 399 329 242 85 28 BspEI T'CCGG_A Cuts at: 6996 6996 Size: 7333 BspHI T'CATG_A Cuts at: 5222 6952 5222 Size: 1730 5603 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2109 2653 3083 4688 844 Size: 1265 544 430 1605 3489 Fragments arranged by size: 3489 1605 1265 544 430 BsrBI CCG'CTC Cuts at: 1394 1823 5220 1394 Size: 429 3397 3507 Fragments arranged by size: 3507 3397 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2811 4413 5828 6002 65 Size: 1183 1563 1602 1415 174 1396 Fragments arranged by size: 1602 1563 1415 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2316 5977 6164 1700 Size: 110 506 3661 187 2869 Fragments arranged by size: 3661 2869 506 187 110 BsrGI T'GTAC_A Cuts at: 96 4481 96 Size: 4385 2948 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7333 BssSI C'ACGA_G Cuts at: 5393 6405 5393 Size: 1012 6321 BstAPI GCAn_nnn'nTGC Cuts at: 794 3464 794 Size: 2670 4663 BstBI TT'CG_AA Cuts at: 6137 6137 Size: 7333 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7333 BstXI CCAn_nnnn'nTGG Cuts at: 2134 4418 2134 Size: 2284 5049 BstYI r'GATC_y Cuts at: 1972 2209 2364 3810 4663 5030 5415 5432 6884 Size: 237 155 1446 853 367 385 17 1452 Cuts at: 6884 6970 6982 7328 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1446 853 385 367 346 237 155 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3575 3575 Size: 7333 BtgI C'CryG_G Cuts at: 513 1065 2004 2091 513 Size: 552 939 87 5755 Fragments arranged by size: 5755 939 552 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6594 Fragments arranged by size: 6594 683 56 BtsI GCAGTG_nn' Cuts at: 3168 4849 5643 5663 3168 Size: 1681 794 20 4838 Fragments arranged by size: 4838 1681 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7333 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2095 2130 529 Size: 35 1531 35 5732 Fragments arranged by size: 5732 1531 35 35 DraI TTT'AAA Cuts at: 3955 4189 4637 3955 Size: 234 448 6651 Fragments arranged by size: 6651 448 234 DraIII CAC_nnn'GTG Cuts at: 2339 2685 2339 Size: 346 6987 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6340 809 Size: 507 5024 1802 Fragments arranged by size: 5024 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 2381 2406 Size: 54 1425 50 275 18 258 293 25 Cuts at: 2406 2883 3069 4780 5669 7298 8 Size: 477 186 1711 889 1629 43 Fragments arranged by size: 1711 1629 1425 889 477 293 275 258 186 54 50 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 2088 4780 1537 Size: 275 276 2692 4090 Fragments arranged by size: 4090 2692 276 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2550 3771 4224 5263 1353 Size: 1197 1221 453 1039 3423 Fragments arranged by size: 3423 1221 1197 1039 453 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4570 5920 6294 6440 387 Size: 1064 138 2981 1350 374 146 1280 Fragments arranged by size: 2981 1350 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2099 2880 3345 3412 3459 3674 Size: 393 440 27 781 465 67 47 215 Cuts at: 3674 3728 3752 4205 4440 4569 4640 5396 5993 Size: 54 24 453 235 129 71 756 597 Cuts at: 5993 6780 7048 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 781 756 597 465 453 440 393 268 235 215 129 71 67 54 47 27 24 EcoO109I rG'GnC_Cy Cuts at: 2774 2774 Size: 7333 EcoRI G'AATT_C Cuts at: 2846 3929 4642 5020 2846 Size: 1083 713 378 5159 Fragments arranged by size: 5159 1083 713 378 EcoRV GAT'ATC Cuts at: 2218 2218 Size: 7333 FspI TGC'GCA Cuts at: 5839 5839 Size: 7333 HaeII r_GCGC'y Cuts at: 1678 6480 1678 Size: 4802 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2023 2055 2259 2291 2624 Size: 32 135 32 451 32 204 32 333 Cuts at: 2624 2656 3364 3396 3731 3763 5974 6006 6048 Size: 32 708 32 335 32 2211 32 42 Cuts at: 6048 6080 1373 Size: 32 2626 Fragments arranged by size: 2626 2211 708 451 335 333 204 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2451 4545 4677 4923 669 Size: 988 281 513 2094 132 246 3079 Fragments arranged by size: 3079 2094 988 513 281 246 132 HindIII A'AGCT_T Cuts at: 748 2892 748 Size: 2144 5189 HpaI GTT'AAC Cuts at: 4545 4923 4545 Size: 378 6955 KpnI G_GTAC'C Cuts at: 3151 4658 3151 Size: 1507 5826 MfeI C'AATT_G Cuts at: 4932 4932 Size: 7333 MluI A'CGCG_T Cuts at: 7209 7209 Size: 7333 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4077 6447 6631 1305 Size: 2772 2370 184 2007 Fragments arranged by size: 2772 2370 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2383 2408 2885 3071 10 Size: 54 1425 343 551 25 477 186 4272 Fragments arranged by size: 4272 1425 551 477 343 186 54 25 MslI CAynn'nnrTG Cuts at: 518 1166 3798 3908 4359 4437 5291 5650 5809 Size: 648 2632 110 451 78 854 359 159 Cuts at: 5809 518 Size: 2042 Fragments arranged by size: 2632 2042 854 648 451 359 159 110 78 MspA1I CmG'CkG Cuts at: 1606 2174 2401 3287 3860 4013 4087 4749 5426 Size: 568 227 886 573 153 74 662 677 Cuts at: 5426 6574 6819 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 886 677 662 573 568 245 227 153 74 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5842 Fragments arranged by size: 5842 939 552 NdeI CA'TA_TG Cuts at: 387 3589 387 Size: 3202 4131 NheI G'CTAG_C Cuts at: 1052 2578 1052 Size: 1526 5807 NotI GC'GGCC_GC Cuts at: 4780 4780 Size: 7333 NspI r_CATG'y Cuts at: 1132 1229 3104 4691 6236 1132 Size: 97 1875 1587 1545 2229 Fragments arranged by size: 2229 1875 1587 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7333 PciI A'CATG_T Cuts at: 3100 6232 3100 Size: 3132 4201 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7333 PfoI T'CCnGG_A Cuts at: 2076 2143 2076 Size: 67 7266 PmeI GTTT'AAAC Cuts at: 4637 4637 Size: 7333 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5402 5434 6915 6947 5402 Size: 32 1481 32 5788 Fragments arranged by size: 5788 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2774 2774 Size: 7333 PshAI GACnn'nnGTC Cuts at: 1613 3659 1613 Size: 2046 5287 PsiI TTA'TAA Cuts at: 4903 4903 Size: 7333 PstI C_TGCA'G Cuts at: 830 3460 4685 830 Size: 2630 1225 3478 Fragments arranged by size: 3478 2630 1225 PvuI CG_AT'CG Cuts at: 2001 2047 2001 Size: 46 7287 PvuII CAG'CTG Cuts at: 2174 2401 3287 3860 4013 4087 2174 Size: 227 886 573 153 74 5420 Fragments arranged by size: 5420 886 573 227 153 74 SalI G'TCGA_C Cuts at: 1936 2449 4675 1936 Size: 513 2226 4594 Fragments arranged by size: 4594 2226 513 SapI GCTCTTCn'nnn_ Cuts at: 2550 2550 Size: 7333 SbfI CC_TGCA'GG Cuts at: 4685 4685 Size: 7333 ScaI AGT'ACT Cuts at: 5581 7293 5581 Size: 1712 5621 SexAI A'CCwGG_T Cuts at: 3996 3996 Size: 7333 SfcI C'TryA_G Cuts at: 826 1045 2960 3297 3362 3456 3543 4681 5816 Size: 219 1915 337 65 94 87 1138 1135 Cuts at: 5816 6497 6688 826 Size: 681 191 1471 Fragments arranged by size: 1915 1471 1138 1135 681 337 219 191 94 87 65 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7333 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7333 SmaI CCC'GGG Cuts at: 1872 4660 1872 Size: 2788 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2024 3935 5437 6338 6600 820 Size: 197 926 81 1911 1502 901 262 1553 Fragments arranged by size: 1911 1553 1502 926 901 262 197 81 SnaBI TAC'GTA Cuts at: 493 5124 493 Size: 4631 2702 SpeI A'CTAG_T Cuts at: 152 3217 3907 152 Size: 3065 690 3578 Fragments arranged by size: 3578 3065 690 SphI G_CATG'C Cuts at: 1132 4691 1132 Size: 3559 3774 SspI AAT'ATT Cuts at: 5 52 5257 5 Size: 47 5205 2081 Fragments arranged by size: 5205 2081 47 StuI AGG'CCT Cuts at: 1517 3950 4229 1517 Size: 2433 279 4621 Fragments arranged by size: 4621 2433 279 StyI C'CwwG_G Cuts at: 513 1065 2004 3624 4772 513 Size: 552 939 1620 1148 3074 Fragments arranged by size: 3074 1620 1148 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3911 5360 5377 5530 5715 6160 325 Size: 1255 2331 1449 17 153 185 445 1498 Fragments arranged by size: 2331 1498 1449 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2754 3874 3939 Size: 274 80 33 51 1443 777 1120 65 Cuts at: 3939 4481 4501 5579 7291 96 Size: 542 20 1078 1712 138 Fragments arranged by size: 1712 1443 1120 1078 777 542 274 138 80 65 51 33 20 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2671 2703 3090 3122 7293 7325 2671 Size: 32 387 32 4171 32 2679 Fragments arranged by size: 4171 2679 387 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2035 3092 1316 Size: 719 1057 5557 Fragments arranged by size: 5557 1057 719 XbaI T'CTAG_A Cuts at: 4669 4669 Size: 7333 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2113 2376 3857 2113 Size: 263 1481 5589 Fragments arranged by size: 5589 1481 263 XhoI C'TCGA_G Cuts at: 1943 2024 1943 Size: 81 7252 XmaI C'CCGG_G Cuts at: 1870 4658 1870 Size: 2788 4545 XmnI GAAnn'nnTTC Cuts at: 2391 4392 5462 2391 Size: 2001 1070 4262 Fragments arranged by size: 4262 2001 1070 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5141 276 Size: 53 83 186 936 3607 2468 Fragments arranged by size: 3607 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI