Restriction map for pFN21AB3411
 (Linear) (Six-base) MAPSORT of: KIBB3411.seq  Check: 969  from: 1  to: 2697



KIBB3411.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1445    1583    2697 
   Size:       1445     138    1114

  Fragments arranged by size: 

                1445    1114     138
 
AccI GT'mk_AC

Cuts at:      0    2411    2490    2697 
   Size:       2411      79     207

  Fragments arranged by size: 

                2411     207      79
 
Acc65I G'GTAC_C

Cuts at:      0    2036    2697 
   Size:       2036     661
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445     562     646     703    1729    1892    2083    2171 
   Size:        445     117      84      57    1026     163     191      88

Cuts at:   2171    2697 
   Size:        526

  Fragments arranged by size: 

                1026     526     445     191     163     117      88      84
                  57
 
AflIII A'CryG_T

Cuts at:      0     947    2414    2697 
   Size:        947    1467     283

  Fragments arranged by size: 

                1467     947     283
 
AleI CACnn'nnGTG

Cuts at:      0     838    1067    2221    2578    2697 
   Size:        838     229    1154     357     119

  Fragments arranged by size: 

                1154     838     357     229     119
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2467    2697 
   Size:       2467     230
 
AlwNI CAG_nnn'CTG

Cuts at:      0     715    1714    2331    2697 
   Size:        715     999     617     366

  Fragments arranged by size: 

                 999     715     617     366
 
ApaI G_GGCC'C

Cuts at:      0     377    1180    2641    2697 
   Size:        377     803    1461      56

  Fragments arranged by size: 

                1461     803     377      56
 
ApaLI G'TGCA_C

Cuts at:      0    1736    2692    2697 
   Size:       1736     956       5

  Fragments arranged by size: 

                1736     956       5
 
AvaI C'yCGr_G

Cuts at:      0    1194    1328    1373    2555    2697 
   Size:       1194     134      45    1182     142

  Fragments arranged by size: 

                1194    1182     142     134      45
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2020    2053    2697 
   Size:       2020      33     644

  Fragments arranged by size: 

                2020     644      33
 
BanI G'GyrC_C

Cuts at:      0     199    1230    2036    2675    2697 
   Size:        199    1031     806     639      22

  Fragments arranged by size: 

                1031     806     639     199      22
 
BanII G_rGCy'C

Cuts at:      0     377    1180    1329    1380    1758    1830    2102    2641 
   Size:        377     803     149      51     378      72     272     539

Cuts at:   2641    2666    2697 
   Size:         25      31

  Fragments arranged by size: 

                 803     539     378     377     272     149      72      51
                  31      25
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     995    2233    2594    2697 
   Size:        995    1238     361     103

  Fragments arranged by size: 

                1238     995     361     103
 
BbvCI CC'TCA_GC

Cuts at:      0    2539    2697 
   Size:       2539     158
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1309    1343    2697 
   Size:       1309      34    1354

  Fragments arranged by size: 

                1354    1309      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1814    2697 
   Size:       1814     883
 
BclI T'GATC_A

Cuts at:      0    1604    2697 
   Size:       1604    1093
 
BfrBI ATG'CAT

Cuts at:      0    1692    2697 
   Size:       1692    1005
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1184    1352    2697 
   Size:       1184     168    1345

  Fragments arranged by size: 

                1345    1184     168
 
BglII A'GATC_T

Cuts at:      0     242    2697 
   Size:        242    2455
 
Bme1580I G_kGCm'C

Cuts at:      0     377    1180    1235    1740    1769    2110    2641    2678 
   Size:        377     803      55     505      29     341     531      37

Cuts at:   2678    2696    2697 
   Size:         18       1

  Fragments arranged by size: 

                 803     531     505     377     341      55      37      29
                  18       1
 
BmgBI CAC'GTC

Cuts at:      0     948    2697 
   Size:        948    1749
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1284    2041    2432    2697 
   Size:       1284     757     391     265

  Fragments arranged by size: 

                1284     757     391     265
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1399    1772    2697 
   Size:       1399     373     925

  Fragments arranged by size: 

                1399     925     373
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     189     541    1130    1263    2353    2515    2697 
   Size:        189     352     589     133    1090     162     182

  Fragments arranged by size: 

                1090     589     352     189     182     162     133
 
Bpu10I CC'TnA_GC

Cuts at:      0     137    1724    2539    2648    2697 
   Size:        137    1587     815     109      49

  Fragments arranged by size: 

                1587     815     137     109      49
 
BsaAI yAC'GTr

Cuts at:      0    2417    2697 
   Size:       2417     280
 
BsaHI Gr'CG_yC

Cuts at:      0    1394    1585    2697 
   Size:       1394     191    1112

  Fragments arranged by size: 

                1394    1112     191
 
BsaWI w'CCGG_w

Cuts at:      0     866    2697 
   Size:        866    1831
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     719     749     932     962    1927    1957    2089    2119 
   Size:        719      30     183      30     965      30     132      30

Cuts at:   2119    2488    2518    2697 
   Size:        369      30     179

  Fragments arranged by size: 

                 965     719     369     183     179     132      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      54     953    1118    1127    1131    1948    2110    2536 
   Size:         54     899     165       9       4     817     162     426

Cuts at:   2536    2697 
   Size:        161

  Fragments arranged by size: 

                 899     817     426     165     162     161      54       9
                   4
 
BseYI C'CCAG_C

Cuts at:      0     348     641    1040    1234    1656    1769    1938    2281 
   Size:        348     293     399     194     422     113     169     343

Cuts at:   2281    2286    2369    2569    2634    2681    2697 
   Size:          5      83     200      65      47      16

  Fragments arranged by size: 

                 422     399     348     343     293     200     194     169
                 113      83      65      47      16       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     778    1024    1106    1459    1561    1576    1670    1999 
   Size:        778     246      82     353     102      15      94     329

Cuts at:   1999    2697 
   Size:        698

  Fragments arranged by size: 

                 778     698     353     329     246     102      94      82
                  15
 
BsiHKAI G_wGCw'C

Cuts at:      0     312    1329    1722    1740    1758    2696    2697 
   Size:        312    1017     393      18      18     938       1

  Fragments arranged by size: 

                1017     938     393     312      18      18       1
 
BsiWI C'GTAC_G

Cuts at:      0    2597    2697 
   Size:       2597     100
 
Bsp1286I G_dGCh'C

Cuts at:      0     312     377    1180    1235    1329    1380    1722    1740 
   Size:        312      65     803      55      94      51     342      18

Cuts at:   1740    1758    1769    1830    2102    2110    2641    2666    2678 
   Size:         18      11      61     272       8     531      25      12

Cuts at:   2678    2696    2697 
   Size:         18       1

  Fragments arranged by size: 

                 803     531     342     312     272      94      65      61
                  55      51      25      18      18      18      12      11
                   8       1
 
BspEI T'CCGG_A

Cuts at:      0     866    2697 
   Size:        866    1831
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1445    1583    2476    2697 
   Size:       1445     138     893     221

  Fragments arranged by size: 

                1445     893     221     138
 
BsrBI CCG'CTC

Cuts at:      0     479     900    1890    2697 
   Size:        479     421     990     807

  Fragments arranged by size: 

                 990     807     479     421
 
BsrDI GCAATG_nn'

Cuts at:      0     343    2697 
   Size:        343    2354
 
BssHII G'CGCG_C

Cuts at:      0    1342    1408    1410    1412    2697 
   Size:       1342      66       2       2    1285

  Fragments arranged by size: 

                1342    1285      66       2       2
 
BssSI C'ACGA_G

Cuts at:      0     305    2697 
   Size:        305    2392
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     338    2697 
   Size:        338    2359
 
BstEII G'GTnAC_C

Cuts at:      0    1947    1959    2697 
   Size:       1947      12     738

  Fragments arranged by size: 

                1947     738      12
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1069    1695    1929    2697 
   Size:       1069     626     234     768

  Fragments arranged by size: 

                1069     768     626     234
 
BstYI r'GATC_y

Cuts at:      0     242     353     555    1848    2697 
   Size:        242     111     202    1293     849

  Fragments arranged by size: 

                1293     849     242     202     111
 
Bsu36I CC'TnA_GG

Cuts at:      0    2687    2697 
   Size:       2687      10
 
BtgI C'CryG_G

Cuts at:      0     183    1181    1226    1259    1400    1491    2258    2670 
   Size:        183     998      45      33     141      91     767     412

Cuts at:   2670    2697 
   Size:         27

  Fragments arranged by size: 

                 998     767     412     183     141      91      45      33
                  27
 
BtsI GCAGTG_nn'

Cuts at:      0    1471    1738    2110    2697 
   Size:       1471     267     372     587

  Fragments arranged by size: 

                1471     587     372     267
 
DraIII CAC_nnn'GTG

Cuts at:      0     295     307    2311    2440    2697 
   Size:        295      12    2004     129     257

  Fragments arranged by size: 

                2004     295     257     129      12
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1393    2697 
   Size:       1393    1304
 
EaeI y'GGCC_r

Cuts at:      0    1220    2697 
   Size:       1220    1477
 
EarI CTCTTCn'nnn_

Cuts at:      0     992    2697 
   Size:        992    1705
 
EcoICRI GAG'CTC

Cuts at:      0    1327    1756    2697 
   Size:       1327     429     941

  Fragments arranged by size: 

                1327     941     429
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     189     445     541     562     646     703    1130    1263 
   Size:        189     256      96      21      84      57     427     133

Cuts at:   1263    1729    1892    2083    2171    2353    2515    2697 
   Size:        466     163     191      88     182     162     182

  Fragments arranged by size: 

                 466     427     256     191     189     182     182     163
                 162     133      96      88      84      57      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     499    2062    2521    2697 
   Size:        499    1563     459     176

  Fragments arranged by size: 

                1563     499     459     176
 
EcoO109I rG'GnC_Cy

Cuts at:      0     175     373     485     492    1177    1283    1305    1545 
   Size:        175     198     112       7     685     106      22     240

Cuts at:   1545    2155    2478    2499    2697 
   Size:        610     323      21     198

  Fragments arranged by size: 

                 685     610     323     240     198     198     175     112
                 106      22      21       7
 
FspI TGC'GCA

Cuts at:      0    2401    2455    2697 
   Size:       2401      54     242

  Fragments arranged by size: 

                2401     242      54
 
HaeII r_GCGC'y

Cuts at:      0    1274    1373    1434    1702    1715    2697 
   Size:       1274      99      61     268      13     982

  Fragments arranged by size: 

                1274     982     268      99      61      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     646     678    2125    2131    2157    2163    2488    2520 
   Size:        646      32    1447       6      26       6     325      32

Cuts at:   2520    2697 
   Size:        177

  Fragments arranged by size: 

                1447     646     325     177      32      32      26       6
                   6
 
HincII GTy'rAC

Cuts at:      0    1560    2697 
   Size:       1560    1137
 
KpnI G_GTAC'C

Cuts at:      0    2040    2697 
   Size:       2040     657
 
MslI CAynn'nnrTG

Cuts at:      0     838    1067    1927    2221    2578    2697 
   Size:        838     229     860     294     357     119

  Fragments arranged by size: 

                 860     838     357     294     229     119
 
MspA1I CmG'CkG

Cuts at:      0     103     471     513    1008    1261    1402    1773    2026 
   Size:        103     368      42     495     253     141     371     253

Cuts at:   2026    2340    2369    2697 
   Size:        314      29     328

  Fragments arranged by size: 

                 495     371     368     328     314     253     253     141
                 103      42      29
 
NcoI C'CATG_G

Cuts at:      0     183    1181    2258    2697 
   Size:        183     998    1077     439

  Fragments arranged by size: 

                1077     998     439     183
 
NdeI CA'TA_TG

Cuts at:      0    1781    2697 
   Size:       1781     916
 
NsiI A_TGCA'T

Cuts at:      0    1694    2697 
   Size:       1694    1003
 
NspI r_CATG'y

Cuts at:      0     421    2697 
   Size:        421    2276
 
PasI CC'CwG_GG

Cuts at:      0     489    2361    2697 
   Size:        489    1872     336

  Fragments arranged by size: 

                1872     489     336
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     324    2613    2697 
   Size:        324    2289      84

  Fragments arranged by size: 

                2289     324      84
 
PfoI T'CCnGG_A

Cuts at:      0    1279    2697 
   Size:       1279    1418
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     766     798    2697 
   Size:        766      32    1899

  Fragments arranged by size: 

                1899     766      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1283    1545    2478    2697 
   Size:       1283     262     933     219

  Fragments arranged by size: 

                1283     933     262     219
 
PshAI GACnn'nnGTC

Cuts at:      0    1504    2697 
   Size:       1504    1193
 
PspOMI G'GGCC_C

Cuts at:      0     373    1176    2637    2697 
   Size:        373     803    1461      60

  Fragments arranged by size: 

                1461     803     373      60
 
PstI C_TGCA'G

Cuts at:      0    1322    2327    2697 
   Size:       1322    1005     370

  Fragments arranged by size: 

                1322    1005     370
 
PvuII CAG'CTG

Cuts at:      0     103    1773    2026    2340    2369    2697 
   Size:        103    1670     253     314      29     328

  Fragments arranged by size: 

                1670     328     314     253     103      29
 
RsrII CG'GwC_CG

Cuts at:      0    1821    2697 
   Size:       1821     876
 
SacI G_AGCT'C

Cuts at:      0    1329    1758    2697 
   Size:       1329     429     939

  Fragments arranged by size: 

                1329     939     429
 
SacII CC_GC'GG

Cuts at:      0    1262    1403    2697 
   Size:       1262     141    1294

  Fragments arranged by size: 

                1294    1262     141
 
SexAI A'CCwGG_T

Cuts at:      0     116     281    2697 
   Size:        116     165    2416

  Fragments arranged by size: 

                2416     165     116
 
SfcI C'TryA_G

Cuts at:      0    1152    1318    2323    2697 
   Size:       1152     166    1005     374

  Fragments arranged by size: 

                1152    1005     374     166
 
SmaI CCC'GGG

Cuts at:      0    1196    2697 
   Size:       1196    1501
 
SmlI C'TyrA_G

Cuts at:      0    1328    2697 
   Size:       1328    1369
 
StuI AGG'CCT

Cuts at:      0     814    1024    2697 
   Size:        814     210    1673

  Fragments arranged by size: 

                1673     814     210
 
StyI C'CwwG_G

Cuts at:      0      80     183     377    1181    2219    2258    2697 
   Size:         80     103     194     804    1038      39     439

  Fragments arranged by size: 

                1038     804     439     194     103      80      39
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     397    1057    1966    2451    2697 
   Size:        397     660     909     485     246

  Fragments arranged by size: 

                 909     660     485     397     246
 
TatI w'GTAC_w

Cuts at:      0    1334    1809    2697 
   Size:       1334     475     888

  Fragments arranged by size: 

                1334     888     475
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     932     964    2089    2121    2697 
   Size:        932      32    1125      32     576

  Fragments arranged by size: 

                1125     932     576      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     273    2697 
   Size:        273    2424
 
XhoI C'TCGA_G

Cuts at:      0    1328    2697 
   Size:       1328    1369
 
XmaI C'CCGG_G

Cuts at:      0    1194    2697 
   Size:       1194    1503
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AleI     AlfI    AlwNI
     ApaI    ApaLI     AvaI     BaeI     BanI    BanII     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI    BsiWI Bsp1286I    BspEI    BspMI
    BsrBI    BsrDI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtgI     BtsI   DraIII     DrdI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I   HincII     KpnI
     MslI   MspA1I     NcoI     NdeI     NsiI     NspI     PasI    PflMI
     PfoI     PpiI    PpuMI    PshAI   PspOMI     PstI    PvuII    RsrII
     SacI    SacII    SexAI     SfcI     SmaI     SmlI     StuI     StyI
    TaqII     TatI     TstI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AhdI     AloI     ApoI
     AscI     AseI    AsiSI    AvrII    BamHI     BbeI     BlpI     BmtI
    BpuEI     BsaI    BsaBI    BsiEI     BsmI    BsmBI    BspHI    BsrFI
    BsrGI    BstBI  BstZ17I    BtgZI     ClaI    CspCI     DraI     EagI
     EciI    EcoRI    EcoRV     FalI     FseI    FspAI  HindIII     HpaI
     KasI     MfeI     MluI     MmeI     MscI     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     PacI     PciI     PmeI     PmlI     PsiI
    PspXI     PsrI     PvuI     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI  Tth111I     XbaI     XmnI     ZraI
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