Restriction map for
pFN21AB3343
##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AclI AcsI AflII AflIII
AgeI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AvrII BamHI BbeI BbsI BcgI
BciVI BclI BfrI BlnI BpuAI
BsaBI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstBI BstXI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
HaeII HindIII HpaI KasI KpnI
KspI MamI MluI MseI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI TaqI Tru9I TthHB8I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 671 GACGTC GACGT^C
AccI 520 GTCTAC GT^[AC][GT]AC
AccIII 2504 TCCGGA T^CCGGA
AcyI 671 GACGTC G[GA]^CG[CT]C
AocI 2359 CCTGAGG CC^T[ACGT]AGG
AspI 2528 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1409 TGCGCA TGC^GCA
BbrPI 1143 CACGTG CAC^GTG
BbvCI 876 GCTGAGG CCTCAGC(-5/-2)
BglI 31 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1677 AGATCT A^GATCT
BlpI 771 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 771 GCTCAGC GC^T[ACGT]AGC
BsaHI 671 GACGTC G[GA]^CG[CT]C
BsaWI 2504 TCCGGA [AT]^CCGG[AT]
BseAI 2504 TCCGGA T^CCGGA
BsmBI 960 CGTCTC CGTCTC(1/5)
BspEI 2504 TCCGGA T^CCGGA
BsrBI 1797 CCGCTC CCGCTC(-3/-3)
BssHII 1291 GCGCGC G^CGCGC
BstEII 2333 GGTCACC G^GT[ACGT]ACC
BstPI 2333 GGTCACC G^GT[ACGT]ACC
Bsu36I 2359 CCTGAGG CC^T[ACGT]AGG
CelII 771 GCTCAGC GC^T[ACGT]AGC
Eco81I 2359 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2333 GGTCACC G^GT[ACGT]ACC
FspI 1409 TGCGCA TGC^GCA
HgaI 550 GACGC GACGC(5/10)
Hin1I 671 GACGTC G[GA]^CG[CT]C
MfeI 2312 CAATTG C^AATTG
MroI 2504 TCCGGA T^CCGGA
MunI 2312 CAATTG C^AATTG
NcoI 26 CCATGG C^CATGG
PflFI 2528 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1143 CACGTG CAC^GTG
PmlI 1143 CACGTG CAC^GTG
SapI 2616 GCTCTTC GCTCTTC(1/4)
SbfI 131 CCTGCAGG CCTGCA^GG
SfiI 30 GGCCCTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2523 CCCGGG CCC^GGG
Sse8387I 131 CCTGCAGG CCTGCA^GG
StuI 349 AGGCCT AGG^CCT
Tsp509I 2313 AATT ^AATT
Tth111I 2528 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2523 CCCGGG C^CCGGG
XmaI 2523 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 939 CGCG CG^CG
1292 CGCG
AhdI 1303 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2641 GACTACCTGTC
ApaI 1771 GGGCCC GGGCC^C
2485 GGGCCC
AspEI 1303 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2641 GACTACCTGTC
BalI 803 TGGCCA TGG^CCA
2243 TGGCCA
BsaAI 622 TACGTG [CT]AC^GT[GA]
1143 CACGTG
BsaI 1165 GAGACC GGTCTC(1/5)
813 GGTCTC
BsrFI 1255 ACCGGC [GA]^CCGG[CT]
2321 GCCGGT
BstAPI 90 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
600 GCACCTCATGC
BstUI 939 CGCG CG^CG
1292 CGCG
Cfr10I 1255 ACCGGC [GA]^CCGG[CT]
2321 GCCGGT
Eam1105I 1303 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2641 GACTACCTGTC
EarI 2617 CTCTTC CTCTTC(1/4)
859 GAAGAG
EcoNI 1211 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2550 CCTAGACCAGG
Ksp632I 2617 CTCTTC CTCTTC(1/4)
859 GAAGAG
MluNI 803 TGGCCA TGG^CCA
2243 TGGCCA
MscI 803 TGGCCA TGG^CCA
2243 TGGCCA
MvnI 939 CGCG CG^CG
1292 CGCG
PvuII 774 CAGCTG CAG^CTG
1667 CAGCTG
SexAI 1216 ACCAGGT A^CC[AT]GGT
2147 ACCTGGT
TfiI 921 GATTC G^A[AT]TC
1583 GATTC
XcmI 26 CCATGGCCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
691 CCAGAGGTTTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
TspRI Van91I XhoII