Restriction map for pFN21AB3319
##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AvrII       
BclI        BfrI        BlnI        BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrGI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HincII      HindII      HindIII     
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        NdeI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfuI        SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccI         1879  GTAGAC               GT^[AC][GT]AC
Alw44I        175  GTGCAC               G^TGCAC
AocI         2290  CCTGAGG              CC^T[ACGT]AGG
ApaLI         175  GTGCAC               G^TGCAC
Asp700I       624  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          293  CCCGAG               C^[CT]CG[GA]G
AviII        2006  TGCGCA               TGC^GCA
BbeI         1888  GGCGCC               GGCGC^C
BbsI          519  GAAGAC               GAAGAC(2/6)
BbvCI        2176  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1039  GCATCCAAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2256  GGATAC               GTATCC(6/5)
BpuAI         519  GAAGAC               GAAGAC(2/6)
BsoBI         293  CCCGAG               C^[CT]CG[GA]G
BsrBI        1491  CCGCTC               CCGCTC(-3/-3)
BstAPI       2314  GCAGCAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       2290  CCTGAGG              CC^T[ACGT]AGG
Eco81I       2290  CCTGAGG              CC^T[ACGT]AGG
FspI         2006  TGCGCA               TGC^GCA
KasI         1888  GGCGCC               G^GCGCC
MseI          314  TTAA                 T^TAA
NarI         1888  GGCGCC               GG^CGCC
NcoI         1001  CCATGG               C^CATGG
NheI          859  GCTAGC               G^CTAGC
NspI         1076  GCATGC               [GA]CATG^[CT]
SapI          880  GAAGAGC              GCTCTTC(1/4)
SfiI         2404  GGCCCCCTTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1888  GGCGCC               GGC^GCC
SgrAI        1333  CACCGGCG             C[GA]^CCGG[CT]G
SmlI          318  CTTGAG               C^T[CT][GA]AG
SphI         1076  GCATGC               GCATG^C
Tru9I         314  TTAA                 T^TAA
Tsp509I      2234  AATT                 ^AATT
XcmI          676  CCACCTAAAGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          624  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AcyI         1270  GACGCC               G[GA]^CG[CT]C
             1888  GGCGCC              
AspI          226  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              775  GACCGCGTC           
BamHI        1149  GGATCC               G^GATCC
             2166  GGATCC              
BglI         1744  GCCGGCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2405  GCCCCCTTGGC         
BglII         257  AGATCT               A^GATCT
              356  AGATCT              
BsaHI        1270  GACGCC               G[GA]^CG[CT]C
             1888  GGCGCC              
Hin1I        1270  GACGCC               G[GA]^CG[CT]C
             1888  GGCGCC              
NaeI         1744  GCCGGC               GCC^GGC
             2110  GCCGGC              
NgoMIV       1744  GCCGGC               G^CCGGC
             2110  GCCGGC              
PflFI         226  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              775  GACCGCGTC           
PstI         1597  CTGCAG               CTGCA^G
             2115  CTGCAG              
SacI          595  GAGCTC               GAGCT^C
             1981  GAGCTC              
SfcI         1597  CTGCAG               C^T[GA][CT]AG
             2115  CTGCAG              
TaqI         1048  TCGA                 T^CGA
             1707  TCGA                
Tth111I       226  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              775  GACCGCGTC           
TthHB8I      1048  TCGA                 T^CGA
             1707  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AluI        
AlwI        AlwNI       Aor51HI     ApaI        AspHI       
AvaII       BalI        BanI        BanII       BbrPI       
BbvI        BcnI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BsaAI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrFI       
BsrI        BssHII      BssKI       BssSI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco47III    EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PmaCI       PmlI        PpuMI       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TfiI        TseI        Tsp45I      TspRI       Van91I      
XhoII       
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