Restriction map for pFN21AB3319
 (Linear) (Six-base) MAPSORT of: KIBB3319.seq  Check: 9709  from: 1  to: 2418



KIBB3319.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     188    1437    1905    2418 
   Size:        188    1249     468     513

  Fragments arranged by size: 

                1249     513     468     188
 
AccI GT'mk_AC

Cuts at:      0    1880    2418 
   Size:       1880     538
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403    1120    1744    2418 
   Size:        403     717     624     674

  Fragments arranged by size: 

                 717     674     624     403
 
AfeI AGC'GCT

Cuts at:      0    1351    1777    2101    2418 
   Size:       1351     426     324     317

  Fragments arranged by size: 

                1351     426     324     317
 
AflIII A'CryG_T

Cuts at:      0      62     474    1611    2418 
   Size:         62     412    1137     807

  Fragments arranged by size: 

                1137     807     412      62
 
AleI CACnn'nnGTG

Cuts at:      0    2054    2418 
   Size:       2054     364
 
AlwNI CAG_nnn'CTG

Cuts at:      0     130     378    1548    2320    2418 
   Size:        130     248    1170     772      98

  Fragments arranged by size: 

                1170     772     248     130      98
 
ApaI G_GGCC'C

Cuts at:      0     151    1262    1683    2407    2418 
   Size:        151    1111     421     724      11

  Fragments arranged by size: 

                1111     724     421     151      11
 
ApaLI G'TGCA_C

Cuts at:      0     175    2418 
   Size:        175    2243
 
AvaI C'yCGr_G

Cuts at:      0     293    2418 
   Size:        293    2125
 
BamHI G'GATC_C

Cuts at:      0    1149    2166    2418 
   Size:       1149    1017     252

  Fragments arranged by size: 

                1149    1017     252
 
BanI G'GyrC_C

Cuts at:      0     571     742    1888    2056    2418 
   Size:        571     171    1146     168     362

  Fragments arranged by size: 

                1146     571     362     171     168
 
BanII G_rGCy'C

Cuts at:      0     151     599    1262    1285    1683    1985    2407    2418 
   Size:        151     448     663      23     398     302     422      11

  Fragments arranged by size: 

                 663     448     422     398     302     151      23      11
            
 
BbeI G_GCGC'C

Cuts at:      0    1892    2418 
   Size:       1892     526
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     526    2418 
   Size:        526    1892
 
BbvCI CC'TCA_GC

Cuts at:      0    2177    2418 
   Size:       2177     241
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1028    1062    2418 
   Size:       1028      34    1356

  Fragments arranged by size: 

                1356    1028      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2250    2418 
   Size:       2250     168
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1750    2411    2418 
   Size:       1750     661       7

  Fragments arranged by size: 

                1750     661       7
 
BglII A'GATC_T

Cuts at:      0     257     356    2418 
   Size:        257      99    2062

  Fragments arranged by size: 

                2062     257      99
 
BlpI GC'TnA_GC

Cuts at:      0     265    1789    2096    2103    2418 
   Size:        265    1524     307       7     315

  Fragments arranged by size: 

                1524     315     307     265       7
 
Bme1580I G_kGCm'C

Cuts at:      0     151     179     239     576     747     962    1262    1683 
   Size:        151      28      60     337     171     215     300     421

Cuts at:   1683    2361    2407    2418 
   Size:        678      46      11

  Fragments arranged by size: 

                 678     421     337     300     215     171     151      60
                  46      28      11
 
BmgBI CAC'GTC

Cuts at:      0      63    2418 
   Size:         63    2355
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     546     615     786    2418 
   Size:        546      69     171    1632

  Fragments arranged by size: 

                1632     546     171      69
 
BmtI G_CTAG'C

Cuts at:      0     863    2418 
   Size:        863    1555
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1821    2418 
   Size:       1821     597
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     156     400     571     649    1945    2074    2172    2260 
   Size:        156     244     171      78    1296     129      98      88

Cuts at:   2260    2287    2353    2418 
   Size:         27      66      65

  Fragments arranged by size: 

                1296     244     171     156     129      98      88      78
                  66      65      27
 
Bpu10I CC'TnA_GC

Cuts at:      0    2177    2224    2418 
   Size:       2177      47     194

  Fragments arranged by size: 

                2177     194      47
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     339    2418 
   Size:        339    2079
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     496    1219    1792    2418 
   Size:        496     723     573     626

  Fragments arranged by size: 

                 723     626     573     496
 
BsaAI yAC'GTr

Cuts at:      0     213     475    1612    1908    2418 
   Size:        213     262    1137     296     510

  Fragments arranged by size: 

                1137     510     296     262     213
 
BsaHI Gr'CG_yC

Cuts at:      0    1271    1889    2418 
   Size:       1271     618     529

  Fragments arranged by size: 

                1271     618     529
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1120    1150    2418 
   Size:       1120      30    1268

  Fragments arranged by size: 

                1268    1120      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     153     178     733     862    1141    1225    1360    1750 
   Size:        153      25     555     129     279      84     135     390

Cuts at:   1750    2380    2418 
   Size:        630      38

  Fragments arranged by size: 

                 630     555     390     279     153     135     129      84
                  38      25
 
BseYI C'CCAG_C

Cuts at:      0     955    1548    1671    2418 
   Size:        955     593     123     747

  Fragments arranged by size: 

                 955     747     593     123
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     367     391     853    1072    1315    1540    1567    1930 
   Size:        367      24     462     219     243     225      27     363

Cuts at:   1930    2418 
   Size:        488

  Fragments arranged by size: 

                 488     462     367     363     243     225     219      27
                  24
 
BsiHKAI G_wGCw'C

Cuts at:      0     179     599    1370    1985    2342    2418 
   Size:        179     420     771     615     357      76

  Fragments arranged by size: 

                 771     615     420     357     179      76
 
Bsp1286I G_dGCh'C

Cuts at:      0     151     179     239     576     599     747     962    1262 
   Size:        151      28      60     337      23     148     215     300

Cuts at:   1262    1285    1370    1683    1985    2342    2361    2407    2418 
   Size:         23      85     313     302     357      19      46      11

  Fragments arranged by size: 

                 357     337     313     302     300     215     151     148
                  85      60      46      28      23      23      19      11
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     188    1094    1437    1905    2089    2418 
   Size:        188     906     343     468     184     329

  Fragments arranged by size: 

                 906     468     343     329     188     184
 
BsrBI CCG'CTC

Cuts at:      0    1493    2418 
   Size:       1493     925
 
BsrFI r'CCGG_y

Cuts at:      0    1334    1631    1744    2110    2418 
   Size:       1334     297     113     366     308

  Fragments arranged by size: 

                1334     366     308     297     113
 
BssHII G'CGCG_C

Cuts at:      0      44    2069    2071    2418 
   Size:         44    2025       2     347

  Fragments arranged by size: 

                2025     347      44       2
 
BssSI C'ACGA_G

Cuts at:      0    1069    1090    2362    2418 
   Size:       1069      21    1272      56

  Fragments arranged by size: 

                1272    1069      56      21
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2320    2418 
   Size:       2320      98
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     432     924    1050    2418 
   Size:        432     492     126    1368

  Fragments arranged by size: 

                1368     492     432     126
 
BstYI r'GATC_y

Cuts at:      0     257     356    1149    1184    2166    2418 
   Size:        257      99     793      35     982     252

  Fragments arranged by size: 

                 982     793     257     252      99      35
 
Bsu36I CC'TnA_GG

Cuts at:      0    2291    2418 
   Size:       2291     127
 
BtgI C'CryG_G

Cuts at:      0    1001    1286    1868    2418 
   Size:       1001     285     582     550

  Fragments arranged by size: 

                1001     582     550     285
 
BtsI GCAGTG_nn'

Cuts at:      0     583     854    1495    1606    2418 
   Size:        583     271     641     111     812

  Fragments arranged by size: 

                 812     641     583     271     111
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1743    1778    2418 
   Size:       1743      35     640

  Fragments arranged by size: 

                1743     640      35
 
EaeI y'GGCC_r

Cuts at:      0       2     116     942    1391    1697    1751    2039    2418 
   Size:          2     114     826     449     306      54     288     379

  Fragments arranged by size: 

                 826     449     379     306     288     114      54       2
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     875    1024    1414    2418 
   Size:        875     149     390    1004

  Fragments arranged by size: 

                1004     875     390     149
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     682    2418 
   Size:        682    1736
 
EcoICRI GAG'CTC

Cuts at:      0     597    1983    2418 
   Size:        597    1386     435

  Fragments arranged by size: 

                1386     597     435
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     156     400     403     571     649    1120    1744    1945 
   Size:        156     244       3     168      78     471     624     201

Cuts at:   1945    2074    2172    2260    2287    2353    2418 
   Size:        129      98      88      27      66      65

  Fragments arranged by size: 

                 624     471     244     201     168     156     129      98
                  88      78      66      65      27       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     769    1090    2332    2418 
   Size:        769     321    1242      86

  Fragments arranged by size: 

                1242     769     321      86
 
EcoO109I rG'GnC_Cy

Cuts at:      0     148     429     909    1095    1258    1679    1680    2282 
   Size:        148     281     480     186     163     421       1     602

Cuts at:   2282    2295    2404    2418 
   Size:         13     109      14

  Fragments arranged by size: 

                 602     480     421     281     186     163     148     109
                  14      13       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     543     645    2418 
   Size:        543     102    1773

  Fragments arranged by size: 

                1773     543     102
 
FspI TGC'GCA

Cuts at:      0    2008    2418 
   Size:       2008     410
 
HaeII r_GCGC'y

Cuts at:      0    1296    1353    1779    1892    1945    2021    2103    2418 
   Size:       1296      57     426     113      53      76      82     315

  Fragments arranged by size: 

                1296     426     315     113      82      76      57      53
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2178    2210    2418 
   Size:       2178      32     208

  Fragments arranged by size: 

                2178     208      32
 
KasI G'GCGC_C

Cuts at:      0    1888    2418 
   Size:       1888     530
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1920    2418 
   Size:       1920     498
 
MscI TGG'CCA

Cuts at:      0       4     118    1393    1753    2418 
   Size:          4     114    1275     360     665

  Fragments arranged by size: 

                1275     665     360     114       4
 
MslI CAynn'nnrTG

Cuts at:      0     539    1849    2054    2418 
   Size:        539    1310     205     364

  Fragments arranged by size: 

                1310     539     364     205
 
MspA1I CmG'CkG

Cuts at:      0     201     308     398    1329    1548    1793    2134    2202 
   Size:        201     107      90     931     219     245     341      68

Cuts at:   2202    2418 
   Size:        216

  Fragments arranged by size: 

                 931     341     245     219     216     201     107      90
                  68
 
NaeI GCC'GGC

Cuts at:      0    1746    2112    2418 
   Size:       1746     366     306

  Fragments arranged by size: 

                1746     366     306
 
NarI GG'CG_CC

Cuts at:      0    1889    2418 
   Size:       1889     529
 
NcoI C'CATG_G

Cuts at:      0    1001    2418 
   Size:       1001    1417
 
NgoMIV G'CCGG_C

Cuts at:      0    1744    2110    2418 
   Size:       1744     366     308

  Fragments arranged by size: 

                1744     366     308
 
NheI G'CTAG_C

Cuts at:      0     859    2418 
   Size:        859    1559
 
NspI r_CATG'y

Cuts at:      0    1080    2418 
   Size:       1080    1338
 
PasI CC'CwG_GG

Cuts at:      0    2142    2400    2418 
   Size:       2142     258      18

  Fragments arranged by size: 

                2142     258      18
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     201     498    1548    2418 
   Size:        201     297    1050     870

  Fragments arranged by size: 

                1050     870     297     201
 
PfoI T'CCnGG_A

Cuts at:      0    1625    2418 
   Size:       1625     793
 
PmlI CAC'GTG

Cuts at:      0     475    1612    1908    2418 
   Size:        475    1137     296     510

  Fragments arranged by size: 

                1137     510     475     296
 
PpuMI rG'GwC_Cy

Cuts at:      0     429     909    1095    2295    2418 
   Size:        429     480     186    1200     123

  Fragments arranged by size: 

                1200     480     429     186     123
 
PspOMI G'GGCC_C

Cuts at:      0     147    1258    1679    2403    2418 
   Size:        147    1111     421     724      15

  Fragments arranged by size: 

                1111     724     421     147      15
 
PstI C_TGCA'G

Cuts at:      0    1601    2119    2418 
   Size:       1601     518     299

  Fragments arranged by size: 

                1601     518     299
 
PvuII CAG'CTG

Cuts at:      0     201     308    1329    1548    2134    2202    2418 
   Size:        201     107    1021     219     586      68     216

  Fragments arranged by size: 

                1021     586     219     216     201     107      68
 
SacI G_AGCT'C

Cuts at:      0     599    1985    2418 
   Size:        599    1386     433

  Fragments arranged by size: 

                1386     599     433
 
SapI GCTCTTCn'nnn_

Cuts at:      0     875    2418 
   Size:        875    1543
 
SfcI C'TryA_G

Cuts at:      0    1597    2115    2418 
   Size:       1597     518     303

  Fragments arranged by size: 

                1597     518     303
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2411    2418 
   Size:       2411       7
 
SfoI GGC'GCC

Cuts at:      0    1890    2418 
   Size:       1890     528
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1334    2418 
   Size:       1334    1084
 
SmlI C'TyrA_G

Cuts at:      0     318    2418 
   Size:        318    2100
 
SphI G_CATG'C

Cuts at:      0    1080    2418 
   Size:       1080    1338
 
StyI C'CwwG_G

Cuts at:      0      56     866     890    1001    1511    1554    2409    2418 
   Size:         56     810      24     111     510      43     855       9

  Fragments arranged by size: 

                 855     810     510     111      56      43      24       9
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     730    2418 
   Size:        730    1688
 
TatI w'GTAC_w

Cuts at:      0     167    1136    2418 
   Size:        167     969    1282

  Fragments arranged by size: 

                1282     969     167
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     726     758    1418    1450    2418 
   Size:        726      32     660      32     968

  Fragments arranged by size: 

                 968     726     660      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     229     778    2418 
   Size:        229     549    1640

  Fragments arranged by size: 

                1640     549     229
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     683    2418 
   Size:        683    1735
 
XmnI GAAnn'nnTTC

Cuts at:      0     628    2418 
   Size:        628    1790
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AleI    AlwNI     ApaI
    ApaLI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrBI
    BsrFI   BssHII    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
     BtsI    CspCI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI     FspI    HaeII    Hin4I     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NspI
     PasI    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII
     SacI     SapI     SfcI     SfiI     SfoI    SgrAI     SmlI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AlfI     AloI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BclI    BfrBI
    BsaBI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrDI    BsrGI    BstBI   BstEII  BstZ17I    BtgZI     ClaI     DraI
   DraIII     DrdI     EagI    EcoRI    EcoRV     FseI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     NdeI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SgfI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI     XbaI     XhoI     XmaI     ZraI
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