Restriction map for pFN21AB3312
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BanI        
BbeI        BbrPI       BbvCI       BcgI        BclI        
BcnI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstEII      BstPI       BstUI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco52I      
EcoNI       EcoO65I     EcoRI       FauI        FbaI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HpaI        KasI        KpnI        KspI        
MaeII       MluI        MluNI       MroI        MscI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SmlI        
SnaBI       SphI        Sse8387I    SspBI       Tth111I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AflIII        366  ACATGT               A^C[GA][CT]GT
AlwNI         485  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1078  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      1980  AGCGCT               AGC^GCT
BamHI         883  GGATCC               G^GATCC
BbsI          994  GTCTTC               GAAGAC(2/6)
BciVI        2067  GGATAC               GTATCC(6/5)
BglII        1610  AGATCT               A^GATCT
BpmI          875  CTGGAG               CTGGAG(16/14)
BpuAI         994  GTCTTC               GAAGAC(2/6)
BsaBI        2293  GATTTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          446  GGTCTC               GGTCTC(1/5)
BsaWI         937  ACCGGA               [AT]^CCGG[AT]
BseRI        2362  GAGGAG               GAGGAG(10/8)
BspLU11I      366  ACATGT               A^CATGT
BspMI         218  ACCTGC               ACCTGC(4/8)
BsrBI         147  CCGCTC               CCGCTC(-3/-3)
BsrDI         207  GCAATG               GCAATG(2/0)
BstBI        1871  TTCGAA               TT^CGAA
BstXI        1699  CCAGAGGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1078  CCTCAGG              CC^T[ACGT]AGG
CfoI         1981  GCGC                 GCG^C
DraII        1519  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DraIII       1001  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          972  CGGCCA               [CT]^GGCC[GA]
EarI         1299  CTCTTC               CTCTTC(1/4)
Eco47III     1980  AGCGCT               AGC^GCT
Eco81I       1078  CCTCAGG              CC^T[ACGT]AGG
EcoO109I     1519  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRV        1987  GATATC               GAT^ATC
EcoT14I      1166  CCAAGG               C^C[AT][AT]GG
HaeII        1980  AGCGCT               [GA]GCGC^[CT]
HapII         938  CCGG                 C^CGG
HhaI         1981  GCGC                 GCG^C
HinP1I       1981  GCGC                 G^CGC
HindIII       250  AAGCTT               A^AGCTT
HpaII         938  CCGG                 C^CGG
Ksp632I      1299  CTCTTC               CTCTTC(1/4)
MamI         2293  GATTTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI          938  CCGG                 C^CGG
NspV         1871  TTCGAA               TT^CGAA
ScaI          668  AGTACT               AGT^ACT
SfuI         1871  TTCGAA               TT^CGAA
SpeI         1147  ACTAGT               A^CTAGT
StyI         1166  CCAAGG               C^C[AT][AT]GG
SwaI          501  ATTTAAAT             ATTT^AAAT
Tsp45I       1151  GTGAC                ^GT[GC]AC
XcmI         1482  CCACTACAATACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

Asp700I      1127  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1722  GAACTGTTTC          
AvaII         811  GGACC                G^G[AT]CC
             1057  GGTCC               
BanII         941  GAGCCC               G[GA]GC[CT]^C
             1089  GAGCCC              
BfaI         1148  CTAG                 C^TAG
             1895  CTAG                
BmyI          941  GAGCCC               G[AGT]GC[ACT]^C
             1089  GAGCCC              
BsmAI         595  GAGAC                GTCTC(1/5)
              447  GTCTC               
BsmFI         363  GGGAC                GGGAC(10/14)
              733  GTCCC               
Bsp1286I      941  GAGCCC               G[AGT]GC[ACT]^C
             1089  GAGCCC              
BstF5I        932  CATCC                GGATG(2/0)
             2433  GGATG               
DraI          429  TTTAAA               TTT^AAA
              502  TTTAAA              
EcoT22I      1907  ATGCAT               ATGCA^T
             1966  ATGCAT              
FokI          932  CATCC                GGATG(9/13)
             2433  GGATG               
MaeI         1148  CTAG                 C^TAG
             1895  CTAG                
MfeI          626  CAATTG               C^AATTG
             2333  CAATTG              
MslI          203  CACCGCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1900  CATTATAATG          
MspA1I        710  CAGCTG               C[AC]G^C[GT]G
             2453  CAGCTG              
MunI          626  CAATTG               C^AATTG
             2333  CAATTG              
MwoI          160  GCATATAAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1705  GCCTGGTGTGC         
NsiI         1907  ATGCAT               ATGCA^T
             1966  ATGCAT              
PstI           10  CTGCAG               CTGCA^G
             1715  CTGCAG              
PvuII         710  CAGCTG               CAG^CTG
             2453  CAGCTG              
SfcI           10  CTGCAG               C^T[GA][CT]AG
             1715  CTGCAG              
SspI         1456  AATATT               AAT^ATT
             1560  AATATT              
StuI         1349  AGGCCT               AGG^CCT
             1703  AGGCCT              
XmnI         1127  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1722  GAACTGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BbvI        BsaJI       BsiYI       BslI        
BsrI        BssKI       BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       EcoRII      Fnu4HI      
HaeIII      HinfI       HphI        ItaI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MvaI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TaqI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       
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