(Circular) (Six-base) MAPSORT of: pFN21AB3312.seq Check: 6199 from: 1 to: 7177 pFN21AB3312 7177 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4987 278 Size: 53 83 186 936 3451 2468 Fragments arranged by size: 3451 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4520 1937 Size: 2583 4594 Acc65I G'GTAC_C Cuts at: 4498 4498 Size: 7177 AclI AA'CG_TT Cuts at: 1563 5304 5677 1563 Size: 3741 373 3063 Fragments arranged by size: 3741 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3867 5240 6624 6892 1632 Size: 2235 1373 1384 268 1917 Fragments arranged by size: 2235 1917 1384 1373 268 AfeI AGC'GCT Cuts at: 3987 3987 Size: 7177 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6980 AflIII A'CryG_T Cuts at: 1203 2371 6076 7053 1203 Size: 1168 3705 977 1327 Fragments arranged by size: 3705 1327 1168 977 AgeI A'CCGG_T Cuts at: 6008 6008 Size: 7177 AhdI GACnn_n'nnGTC Cuts at: 5906 5906 Size: 7177 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7177 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2240 2558 1184 Size: 1056 318 5803 Fragments arranged by size: 5803 1056 318 AlwNI CAG_nnn'CTG Cuts at: 2495 4920 6492 2495 Size: 2425 1572 3180 Fragments arranged by size: 3180 2425 1572 ApaLI G'TGCA_C Cuts at: 5234 6390 5234 Size: 1156 6021 ApoI r'AATT_y Cuts at: 1493 2510 2650 3697 3767 3953 4121 4279 4472 Size: 1017 140 1047 70 186 168 158 193 Cuts at: 4472 4486 4712 4864 6973 1493 Size: 14 226 152 2109 1697 Fragments arranged by size: 2109 1697 1047 1017 226 193 186 168 158 152 140 70 14 AseI AT'TA_AT Cuts at: 160 1032 5731 160 Size: 872 4699 1606 Fragments arranged by size: 4699 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7177 AvaI C'yCGr_G Cuts at: 1870 1943 4502 1870 Size: 73 2559 4545 Fragments arranged by size: 4545 2559 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2550 2583 2550 Size: 33 7144 BamHI G'GATC_C Cuts at: 2888 4507 4874 2888 Size: 1619 367 5191 Fragments arranged by size: 5191 1619 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4498 5953 618 Size: 325 217 627 2711 1455 1842 Fragments arranged by size: 2711 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2950 3098 4496 1391 Size: 1559 148 1398 4072 Fragments arranged by size: 4072 1559 1398 148 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2992 928 Size: 971 1093 5113 Fragments arranged by size: 5113 1093 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5368 5402 1903 Size: 34 3431 34 3678 Fragments arranged by size: 3678 3431 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4066 5069 6279 4066 Size: 1003 1210 4964 Fragments arranged by size: 4964 1210 1003 BclI T'GATC_A Cuts at: 1548 1548 Size: 7177 BfrBI ATG'CAT Cuts at: 3914 3973 3914 Size: 59 7118 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5788 136 Size: 107 122 71 631 605 4116 1525 Fragments arranged by size: 4116 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3615 7172 3615 Size: 3557 3620 BlpI GC'TnA_GC Cuts at: 4594 4594 Size: 7177 Bme1580I G_kGCm'C Cuts at: 1790 5238 6394 1790 Size: 3448 1156 2573 Fragments arranged by size: 3448 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7177 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2753 4908 5866 Size: 416 53 83 399 24 1336 2155 958 Cuts at: 5866 442 Size: 1753 Fragments arranged by size: 2155 1753 1336 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7177 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3637 3637 Size: 7177 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2901 5837 1239 Size: 1662 2936 2579 Fragments arranged by size: 2936 2579 1662 Bpu10I CC'TnA_GC Cuts at: 2155 2155 Size: 7177 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5302 6167 6465 5302 Size: 865 298 6014 Fragments arranged by size: 6014 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2457 5840 882 Size: 617 958 3383 2219 Fragments arranged by size: 3383 2219 958 617 BsaAI yAC'GTr Cuts at: 493 1204 4968 493 Size: 711 3764 2702 Fragments arranged by size: 3764 2702 711 BsaBI GATnn'nnATC Cuts at: 4302 7171 4302 Size: 2869 4308 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4984 5366 6001 275 Size: 53 83 186 936 3451 382 635 1451 Fragments arranged by size: 3451 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2942 5610 6008 6282 6429 6840 2942 Size: 2668 398 274 147 411 3279 Fragments arranged by size: 3279 2668 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6952 Fragments arranged by size: 6952 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4382 1186 Size: 3196 3981 BseYI C'CCAG_C Cuts at: 6380 6876 6380 Size: 496 6681 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6604 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4627 5388 6416 1540 Size: 275 186 2626 761 1028 2301 Fragments arranged by size: 2626 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5238 5323 6394 5238 Size: 85 1071 6021 Fragments arranged by size: 6021 1071 85 BsmI GAATG_Cn' Cuts at: 4688 4781 4688 Size: 93 7084 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2950 3098 4496 5238 5323 6394 1391 Size: 399 1160 148 1398 742 85 1071 2174 Fragments arranged by size: 2174 1398 1160 1071 742 399 148 85 BspEI T'CCGG_A Cuts at: 6840 6840 Size: 7177 BspHI T'CATG_A Cuts at: 5066 6796 5066 Size: 1730 5447 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2232 4532 844 Size: 1388 2300 3489 Fragments arranged by size: 3489 2300 1388 BsrBI CCG'CTC Cuts at: 1394 1823 2154 5064 1394 Size: 429 331 2910 3507 Fragments arranged by size: 3507 2910 429 331 BsrDI GCAATG_nn' Cuts at: 65 1248 2219 5672 5846 65 Size: 1183 971 3453 174 1396 Fragments arranged by size: 3453 1396 1183 971 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5821 6008 1700 Size: 110 4011 187 2869 Fragments arranged by size: 4011 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7177 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7177 BssSI C'ACGA_G Cuts at: 5237 6249 5237 Size: 1012 6165 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7177 BstBI TT'CG_AA Cuts at: 3877 5981 3877 Size: 2104 5073 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7177 BstXI CCAn_nnnn'nTGG Cuts at: 3711 3711 Size: 7177 BstYI r'GATC_y Cuts at: 1972 2491 2888 3615 4303 4507 4874 5259 5276 Size: 519 397 727 688 204 367 385 17 Cuts at: 5276 6728 6814 6826 7172 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 727 688 519 397 385 367 346 204 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3084 3084 Size: 7177 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6625 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6438 Fragments arranged by size: 6438 683 56 BtsI GCAGTG_nn' Cuts at: 3122 4693 5487 5507 3122 Size: 1571 794 20 4792 Fragments arranged by size: 4792 1571 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7177 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7142 DraI TTT'AAA Cuts at: 2436 2509 4481 2436 Size: 73 1972 5132 Fragments arranged by size: 5132 1972 73 DraIII CAC_nnn'GTG Cuts at: 3011 3011 Size: 7177 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6184 809 Size: 507 4868 1802 Fragments arranged by size: 4868 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2977 4624 5513 Size: 54 1425 50 275 18 1147 1647 889 Cuts at: 5513 7142 8 Size: 1629 43 Fragments arranged by size: 1647 1629 1425 1147 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4624 1537 Size: 275 2812 4090 Fragments arranged by size: 4090 2812 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3310 5107 1353 Size: 1957 1797 3423 Fragments arranged by size: 3423 1957 1797 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5764 6138 6284 387 Size: 1064 138 4175 374 146 1280 Fragments arranged by size: 4175 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2901 3867 5240 5837 6624 6892 1239 Size: 393 1269 966 1373 597 787 268 1524 Fragments arranged by size: 1524 1373 1269 966 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3525 3525 Size: 7177 EcoRI G'AATT_C Cuts at: 4486 4864 4486 Size: 378 6799 EcoRV GAT'ATC Cuts at: 3994 3994 Size: 7177 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2695 3016 2695 Size: 321 6856 FspI TGC'GCA Cuts at: 5683 5683 Size: 7177 HaeII r_GCGC'y Cuts at: 1678 3989 6324 1678 Size: 2311 2335 2531 Fragments arranged by size: 2531 2335 2311 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2921 2953 5818 5850 5892 Size: 32 135 32 1349 32 2865 32 42 Cuts at: 5892 5924 1373 Size: 32 2626 Fragments arranged by size: 2865 2626 1349 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4521 4767 669 Size: 988 281 2583 246 3079 Fragments arranged by size: 3079 2583 988 281 246 HindIII A'AGCT_T Cuts at: 748 2255 748 Size: 1507 5670 HpaI GTT'AAC Cuts at: 4767 4767 Size: 7177 KpnI G_GTAC'C Cuts at: 4502 4502 Size: 7177 MfeI C'AATT_G Cuts at: 2631 4338 4776 2631 Size: 1707 438 5032 Fragments arranged by size: 5032 1707 438 MluI A'CGCG_T Cuts at: 7053 7053 Size: 7177 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6291 6475 1305 Size: 4986 184 2007 Fragments arranged by size: 4986 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5355 Fragments arranged by size: 5355 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2212 3909 5135 5494 5653 518 Size: 648 843 203 1697 1226 359 159 2042 Fragments arranged by size: 2042 1697 1226 843 648 359 203 159 MspA1I CmG'CkG Cuts at: 1606 2717 4460 4593 5270 6418 6663 1606 Size: 1111 1743 133 677 1148 245 2120 Fragments arranged by size: 2120 1743 1148 1111 677 245 133 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6625 NdeI CA'TA_TG Cuts at: 387 387 Size: 7177 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7177 NotI GC'GGCC_GC Cuts at: 4624 4624 Size: 7177 NsiI A_TGCA'T Cuts at: 3916 3975 3916 Size: 59 7118 NspI r_CATG'y Cuts at: 1132 1229 2375 3553 4046 4535 6080 1132 Size: 97 1146 1178 493 489 1545 2229 Fragments arranged by size: 2229 1545 1178 1146 493 489 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7177 PciI A'CATG_T Cuts at: 2371 6076 2371 Size: 3705 3472 PflMI CCAn_nnn'nTGG Cuts at: 1108 2795 2849 2879 1108 Size: 1687 54 30 5406 Fragments arranged by size: 5406 1687 54 30 PfoI T'CCnGG_A Cuts at: 2878 2878 Size: 7177 PmeI GTTT'AAAC Cuts at: 4481 4481 Size: 7177 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5246 5278 6759 6791 5246 Size: 32 1481 32 5632 Fragments arranged by size: 5632 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7177 PsiI TTA'TAA Cuts at: 3909 4747 3909 Size: 838 6339 PstI C_TGCA'G Cuts at: 830 2019 3724 4529 830 Size: 1189 1705 805 3478 Fragments arranged by size: 3478 1705 1189 805 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7177 PvuII CAG'CTG Cuts at: 2717 4460 2717 Size: 1743 5434 SalI G'TCGA_C Cuts at: 1936 4519 1936 Size: 2583 4594 SbfI CC_TGCA'GG Cuts at: 4529 4529 Size: 7177 ScaI AGT'ACT Cuts at: 2675 5425 7137 2675 Size: 2750 1712 2715 Fragments arranged by size: 2750 2715 1712 SfcI C'TryA_G Cuts at: 826 1045 2015 3720 4525 5660 6341 6532 826 Size: 219 970 1705 805 1135 681 191 1471 Fragments arranged by size: 1705 1471 1135 970 805 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7177 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7177 SmaI CCC'GGG Cuts at: 1872 4504 1872 Size: 2632 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5281 6182 6444 820 Size: 197 926 3338 901 262 1553 Fragments arranged by size: 3338 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4968 493 Size: 4475 2702 SpeI A'CTAG_T Cuts at: 152 3152 152 Size: 3000 4177 SphI G_CATG'C Cuts at: 1132 4535 1132 Size: 3403 3774 SspI AAT'ATT Cuts at: 5 52 3463 3567 5101 5 Size: 47 3411 104 1534 2081 Fragments arranged by size: 3411 2081 1534 104 47 StuI AGG'CCT Cuts at: 1517 3356 3710 1517 Size: 1839 354 4984 Fragments arranged by size: 4984 1839 354 StyI C'CwwG_G Cuts at: 513 1065 3171 4616 513 Size: 552 2106 1445 3074 Fragments arranged by size: 3074 2106 1445 552 SwaI ATTT'AAAT Cuts at: 2509 2509 Size: 7177 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5204 5221 5374 5559 6004 325 Size: 1255 3624 17 153 185 445 1498 Fragments arranged by size: 3624 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2107 2201 2673 Size: 274 80 33 51 1443 130 94 472 Cuts at: 2673 3074 3546 5423 7135 96 Size: 401 472 1877 1712 138 Fragments arranged by size: 1877 1712 1443 472 472 401 274 138 130 94 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3198 3230 7137 7169 3198 Size: 32 3907 32 3206 Fragments arranged by size: 3907 3206 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7177 XbaI T'CTAG_A Cuts at: 4513 4513 Size: 7177 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3494 3494 Size: 7177 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7177 XmaI C'CCGG_G Cuts at: 1870 4502 1870 Size: 2632 4545 XmnI GAAnn'nnTTC Cuts at: 3136 3731 5306 3136 Size: 595 1575 5007 Fragments arranged by size: 5007 1575 595 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4985 276 Size: 53 83 186 936 3451 2468 Fragments arranged by size: 3451 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BbvCI BsiWI BsmBI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI