Restriction map for pFN21AB3312
 (Circular) (Six-base) MAPSORT of: pFN21AB3312.seq  Check: 6199  from: 1  to: 7177



pFN21AB3312  7177 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:33  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4987     278 
   Size:         53      83     186     936    3451    2468

  Fragments arranged by size: 

                3451    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    4520    1937 
   Size:       2583    4594
 
Acc65I G'GTAC_C

Cuts at:   4498    4498 
   Size:       7177
 
AclI AA'CG_TT

Cuts at:   1563    5304    5677    1563 
   Size:       3741     373    3063

  Fragments arranged by size: 

                3741    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3867    5240    6624    6892    1632 
   Size:       2235    1373    1384     268    1917

  Fragments arranged by size: 

                2235    1917    1384    1373     268
 
AfeI AGC'GCT

Cuts at:   3987    3987 
   Size:       7177
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6980
 
AflIII A'CryG_T

Cuts at:   1203    2371    6076    7053    1203 
   Size:       1168    3705     977    1327

  Fragments arranged by size: 

                3705    1327    1168     977
 
AgeI A'CCGG_T

Cuts at:   6008    6008 
   Size:       7177
 
AhdI GACnn_n'nnGTC

Cuts at:   5906    5906 
   Size:       7177
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       7177
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2240    2558    1184 
   Size:       1056     318    5803

  Fragments arranged by size: 

                5803    1056     318
 
AlwNI CAG_nnn'CTG

Cuts at:   2495    4920    6492    2495 
   Size:       2425    1572    3180

  Fragments arranged by size: 

                3180    2425    1572
 
ApaLI G'TGCA_C

Cuts at:   5234    6390    5234 
   Size:       1156    6021
 
ApoI r'AATT_y

Cuts at:   1493    2510    2650    3697    3767    3953    4121    4279    4472 
   Size:       1017     140    1047      70     186     168     158     193

Cuts at:   4472    4486    4712    4864    6973    1493 
   Size:         14     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1047    1017     226     193     186     168
                 158     152     140      70      14
 
AseI AT'TA_AT

Cuts at:    160    1032    5731     160 
   Size:        872    4699    1606

  Fragments arranged by size: 

                4699    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7177
 
AvaI C'yCGr_G

Cuts at:   1870    1943    4502    1870 
   Size:         73    2559    4545

  Fragments arranged by size: 

                4545    2559      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2550    2583    2550 
   Size:         33    7144
 
BamHI G'GATC_C

Cuts at:   2888    4507    4874    2888 
   Size:       1619     367    5191

  Fragments arranged by size: 

                5191    1619     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    4498    5953     618 
   Size:        325     217     627    2711    1455    1842

  Fragments arranged by size: 

                2711    1842    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2950    3098    4496    1391 
   Size:       1559     148    1398    4072

  Fragments arranged by size: 

                4072    1559    1398     148
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2992     928 
   Size:        971    1093    5113

  Fragments arranged by size: 

                5113    1093     971
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    5368    5402    1903 
   Size:         34    3431      34    3678

  Fragments arranged by size: 

                3678    3431      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4066    5069    6279    4066 
   Size:       1003    1210    4964

  Fragments arranged by size: 

                4964    1210    1003
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       7177
 
BfrBI ATG'CAT

Cuts at:   3914    3973    3914 
   Size:         59    7118
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    5788     136 
   Size:        107     122      71     631     605    4116    1525

  Fragments arranged by size: 

                4116    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   3615    7172    3615 
   Size:       3557    3620
 
BlpI GC'TnA_GC

Cuts at:   4594    4594 
   Size:       7177
 
Bme1580I G_kGCm'C

Cuts at:   1790    5238    6394    1790 
   Size:       3448    1156    2573

  Fragments arranged by size: 

                3448    2573    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       7177
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2753    4908    5866 
   Size:        416      53      83     399      24    1336    2155     958

Cuts at:   5866     442 
   Size:       1753

  Fragments arranged by size: 

                2155    1753    1336     958     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       7177
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3637    3637 
   Size:       7177
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2901    5837    1239 
   Size:       1662    2936    2579

  Fragments arranged by size: 

                2936    2579    1662
 
Bpu10I CC'TnA_GC

Cuts at:   2155    2155 
   Size:       7177
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   5302    6167    6465    5302 
   Size:        865     298    6014

  Fragments arranged by size: 

                6014     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2457    5840     882 
   Size:        617     958    3383    2219

  Fragments arranged by size: 

                3383    2219     958     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4968     493 
   Size:        711    3764    2702

  Fragments arranged by size: 

                3764    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   4302    7171    4302 
   Size:       2869    4308
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4984    5366    6001     275 
   Size:         53      83     186     936    3451     382     635    1451

  Fragments arranged by size: 

                3451    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2942    5610    6008    6282    6429    6840    2942 
   Size:       2668     398     274     147     411    3279

  Fragments arranged by size: 

                3279    2668     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    6952

  Fragments arranged by size: 

                6952     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    4382    1186 
   Size:       3196    3981
 
BseYI C'CCAG_C

Cuts at:   6380    6876    6380 
   Size:        496    6681
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    6604
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    4627    5388    6416    1540 
   Size:        275     186    2626     761    1028    2301

  Fragments arranged by size: 

                2626    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   5238    5323    6394    5238 
   Size:         85    1071    6021

  Fragments arranged by size: 

                6021    1071      85
 
BsmI GAATG_Cn'

Cuts at:   4688    4781    4688 
   Size:         93    7084
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2950    3098    4496    5238    5323    6394    1391 
   Size:        399    1160     148    1398     742      85    1071    2174

  Fragments arranged by size: 

                2174    1398    1160    1071     742     399     148      85
            
 
BspEI T'CCGG_A

Cuts at:   6840    6840 
   Size:       7177
 
BspHI T'CATG_A

Cuts at:   5066    6796    5066 
   Size:       1730    5447
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2232    4532     844 
   Size:       1388    2300    3489

  Fragments arranged by size: 

                3489    2300    1388
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2154    5064    1394 
   Size:        429     331    2910    3507

  Fragments arranged by size: 

                3507    2910     429     331
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2219    5672    5846      65 
   Size:       1183     971    3453     174    1396

  Fragments arranged by size: 

                3453    1396    1183     971     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5821    6008    1700 
   Size:        110    4011     187    2869

  Fragments arranged by size: 

                4011    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       7177
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       7177
 
BssSI C'ACGA_G

Cuts at:   5237    6249    5237 
   Size:       1012    6165
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       7177
 
BstBI TT'CG_AA

Cuts at:   3877    5981    3877 
   Size:       2104    5073
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       7177
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3711    3711 
   Size:       7177
 
BstYI r'GATC_y

Cuts at:   1972    2491    2888    3615    4303    4507    4874    5259    5276 
   Size:        519     397     727     688     204     367     385      17

Cuts at:   5276    6728    6814    6826    7172    1972 
   Size:       1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1452     727     688     519     397     385     367
                 346     204      86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   3084    3084 
   Size:       7177
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    6625
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    6438

  Fragments arranged by size: 

                6438     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3122    4693    5487    5507    3122 
   Size:       1571     794      20    4792

  Fragments arranged by size: 

                4792    1571     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       7177
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    7142
 
DraI TTT'AAA

Cuts at:   2436    2509    4481    2436 
   Size:         73    1972    5132

  Fragments arranged by size: 

                5132    1972      73
 
DraIII CAC_nnn'GTG

Cuts at:   3011    3011 
   Size:       7177
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    6184     809 
   Size:        507    4868    1802

  Fragments arranged by size: 

                4868    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2977    4624    5513 
   Size:         54    1425      50     275      18    1147    1647     889

Cuts at:   5513    7142       8 
   Size:       1629      43

  Fragments arranged by size: 

                1647    1629    1425    1147     889     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    4624    1537 
   Size:        275    2812    4090

  Fragments arranged by size: 

                4090    2812     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3310    5107    1353 
   Size:       1957    1797    3423

  Fragments arranged by size: 

                3423    1957    1797
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    5764    6138    6284     387 
   Size:       1064     138    4175     374     146    1280

  Fragments arranged by size: 

                4175    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2901    3867    5240    5837    6624    6892    1239 
   Size:        393    1269     966    1373     597     787     268    1524

  Fragments arranged by size: 

                1524    1373    1269     966     787     597     393     268
            
 
EcoO109I rG'GnC_Cy

Cuts at:   3525    3525 
   Size:       7177
 
EcoRI G'AATT_C

Cuts at:   4486    4864    4486 
   Size:        378    6799
 
EcoRV GAT'ATC

Cuts at:   3994    3994 
   Size:       7177
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2695    3016    2695 
   Size:        321    6856
 
FspI TGC'GCA

Cuts at:   5683    5683 
   Size:       7177
 
HaeII r_GCGC'y

Cuts at:   1678    3989    6324    1678 
   Size:       2311    2335    2531

  Fragments arranged by size: 

                2531    2335    2311
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2921    2953    5818    5850    5892 
   Size:         32     135      32    1349      32    2865      32      42

Cuts at:   5892    5924    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2865    2626    1349     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    4521    4767     669 
   Size:        988     281    2583     246    3079

  Fragments arranged by size: 

                3079    2583     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2255     748 
   Size:       1507    5670
 
HpaI GTT'AAC

Cuts at:   4767    4767 
   Size:       7177
 
KpnI G_GTAC'C

Cuts at:   4502    4502 
   Size:       7177
 
MfeI C'AATT_G

Cuts at:   2631    4338    4776    2631 
   Size:       1707     438    5032

  Fragments arranged by size: 

                5032    1707     438
 
MluI A'CGCG_T

Cuts at:   7053    7053 
   Size:       7177
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    6291    6475    1305 
   Size:       4986     184    2007

  Fragments arranged by size: 

                4986    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    5355

  Fragments arranged by size: 

                5355    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2009    2212    3909    5135    5494    5653     518 
   Size:        648     843     203    1697    1226     359     159    2042

  Fragments arranged by size: 

                2042    1697    1226     843     648     359     203     159
            
 
MspA1I CmG'CkG

Cuts at:   1606    2717    4460    4593    5270    6418    6663    1606 
   Size:       1111    1743     133     677    1148     245    2120

  Fragments arranged by size: 

                2120    1743    1148    1111     677     245     133
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    6625
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       7177
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       7177
 
NotI GC'GGCC_GC

Cuts at:   4624    4624 
   Size:       7177
 
NsiI A_TGCA'T

Cuts at:   3916    3975    3916 
   Size:         59    7118
 
NspI r_CATG'y

Cuts at:   1132    1229    2375    3553    4046    4535    6080    1132 
   Size:         97    1146    1178     493     489    1545    2229

  Fragments arranged by size: 

                2229    1545    1178    1146     493     489      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       7177
 
PciI A'CATG_T

Cuts at:   2371    6076    2371 
   Size:       3705    3472
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2795    2849    2879    1108 
   Size:       1687      54      30    5406

  Fragments arranged by size: 

                5406    1687      54      30
 
PfoI T'CCnGG_A

Cuts at:   2878    2878 
   Size:       7177
 
PmeI GTTT'AAAC

Cuts at:   4481    4481 
   Size:       7177
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5246    5278    6759    6791    5246 
   Size:         32    1481      32    5632

  Fragments arranged by size: 

                5632    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       7177
 
PsiI TTA'TAA

Cuts at:   3909    4747    3909 
   Size:        838    6339
 
PstI C_TGCA'G

Cuts at:    830    2019    3724    4529     830 
   Size:       1189    1705     805    3478

  Fragments arranged by size: 

                3478    1705    1189     805
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       7177
 
PvuII CAG'CTG

Cuts at:   2717    4460    2717 
   Size:       1743    5434
 
SalI G'TCGA_C

Cuts at:   1936    4519    1936 
   Size:       2583    4594
 
SbfI CC_TGCA'GG

Cuts at:   4529    4529 
   Size:       7177
 
ScaI AGT'ACT

Cuts at:   2675    5425    7137    2675 
   Size:       2750    1712    2715

  Fragments arranged by size: 

                2750    2715    1712
 
SfcI C'TryA_G

Cuts at:    826    1045    2015    3720    4525    5660    6341    6532     826 
   Size:        219     970    1705     805    1135     681     191    1471

  Fragments arranged by size: 

                1705    1471    1135     970     805     681     219     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7177
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       7177
 
SmaI CCC'GGG

Cuts at:   1872    4504    1872 
   Size:       2632    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    5281    6182    6444     820 
   Size:        197     926    3338     901     262    1553

  Fragments arranged by size: 

                3338    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4968     493 
   Size:       4475    2702
 
SpeI A'CTAG_T

Cuts at:    152    3152     152 
   Size:       3000    4177
 
SphI G_CATG'C

Cuts at:   1132    4535    1132 
   Size:       3403    3774
 
SspI AAT'ATT

Cuts at:      5      52    3463    3567    5101       5 
   Size:         47    3411     104    1534    2081

  Fragments arranged by size: 

                3411    2081    1534     104      47
 
StuI AGG'CCT

Cuts at:   1517    3356    3710    1517 
   Size:       1839     354    4984

  Fragments arranged by size: 

                4984    1839     354
 
StyI C'CwwG_G

Cuts at:    513    1065    3171    4616     513 
   Size:        552    2106    1445    3074

  Fragments arranged by size: 

                3074    2106    1445     552
 
SwaI ATTT'AAAT

Cuts at:   2509    2509 
   Size:       7177
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    5204    5221    5374    5559    6004     325 
   Size:       1255    3624      17     153     185     445    1498

  Fragments arranged by size: 

                3624    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2107    2201    2673 
   Size:        274      80      33      51    1443     130      94     472

Cuts at:   2673    3074    3546    5423    7135      96 
   Size:        401     472    1877    1712     138

  Fragments arranged by size: 

                1877    1712    1443     472     472     401     274     138
                 130      94      80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3198    3230    7137    7169    3198 
   Size:         32    3907      32    3206

  Fragments arranged by size: 

                3907    3206      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       7177
 
XbaI T'CTAG_A

Cuts at:   4513    4513 
   Size:       7177
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3494    3494 
   Size:       7177
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       7177
 
XmaI C'CCGG_G

Cuts at:   1870    4502    1870 
   Size:       2632    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3136    3731    5306    3136 
   Size:        595    1575    5007

  Fragments arranged by size: 

                5007    1575     595
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4985     276 
   Size:         53      83     186     936    3451    2468

  Fragments arranged by size: 

                3451    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NsiI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI
    PvuII     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AloI     ApaI     AscI    AvrII     BbeI    BbvCI    BsiWI
    BsmBI  BstZ17I  EcoICRI    EcoNI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     PacI     PmlI    PpuMI   PspOMI    PspXI
     PsrI    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI
     SfoI     SrfI
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