Restriction map for
pFN21AB3294
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbvCI BfrI BglI BglII BlnI
BsaBI BseAI BsiWI BsmBI BspEI
BspHI BspLU11I BsrDI BstAPI BstBI
Bsu36I CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRV EcoT14I EcoT22I FseI FspI
HindIII HpaI KpnI KspI MamI
MfeI MroI MunI NcoI NdeI
NheI NotI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SnaBI SpeI
Sse8387I StyI SwaI Tth111I Van91I
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 736 GTATAC GT^[AC][GT]AC
BalI 2515 TGGCCA TGG^CCA
BcgI 1097 GCACCGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2524 GTATCC GTATCC(6/5)
BlpI 513 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 513 GCTCAGC GC^T[ACGT]AGC
BsaAI 1519 TACGTG [CT]AC^GT[GA]
BsaI 2761 GAGACC GGTCTC(1/5)
BsiEI 270 CGATCG CG[GA][CT]^CG
BsmI 2477 GCATTC GAATGC(1/-1)
BspDI 268 ATCGAT AT^CGAT
Bst1107I 736 GTATAC GTA^TAC
BstEII 1272 GGTGACC G^GT[ACGT]ACC
BstPI 1272 GGTGACC G^GT[ACGT]ACC
BstZ17I 736 GTATAC GTA^TAC
CelII 513 GCTCAGC GC^T[ACGT]AGC
ClaI 268 ATCGAT AT^CGAT
DraI 2605 TTTAAA TTT^AAA
EarI 497 CTCTTC CTCTTC(1/4)
EcoO65I 1272 GGTGACC G^GT[ACGT]ACC
Ksp632I 497 CTCTTC CTCTTC(1/4)
MluI 1811 ACGCGT A^CGCGT
MluNI 2515 TGGCCA TGG^CCA
MscI 2515 TGGCCA TGG^CCA
NspI 2444 GCATGC [GA]CATG^[CT]
PaeR7I 1703 CTCGAG C^TCGAG
PshAI 1899 GACTCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 270 CGATCG CGAT^CG
PvuII 2595 CAGCTG CAG^CTG
SapI 496 GCTCTTC GCTCTTC(1/4)
SgrAI 1685 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 32 CCCGGG CCC^GGG
SmlI 1703 CTCGAG C^T[CT][GA]AG
SphI 2444 GCATGC GCATG^C
StuI 2322 AGGCCT AGG^CCT
XbaI 433 TCTAGA T^CTAGA
XcmI 1316 CCAGGGTGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1703 CTCGAG C^TCGAG
XmaCI 32 CCCGGG C^CCGGG
XmaI 32 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 1528 GTGCAC G^TGCAC
2458 GTGCAC
AlwNI 148 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1561 CAGCCCCTG
ApaI 2061 GGGCCC GGGCC^C
2286 GGGCCC
ApaLI 1528 GTGCAC G^TGCAC
2458 GTGCAC
BbeI 1698 GGCGCC GGCGC^C
2022 GGCGCC
BbsI 394 GTCTTC GAAGAC(2/6)
2034 GTCTTC
BpuAI 394 GTCTTC GAAGAC(2/6)
2034 GTCTTC
BsaWI 657 ACCGGA [AT]^CCGG[AT]
2635 TCCGGT
BsgI 303 GTGCAG GTGCAG(16/14)
1724 GTGCAG
BsmAI 757 GAGAC GTCTC(1/5)
2761 GAGAC
Bsp1407I 1877 TGTACA T^GTACA
2150 TGTACA
BsrGI 1877 TGTACA T^GTACA
2150 TGTACA
BstXI 2123 CCAGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2225 CCAGCGCGTTGG
EcoRI 258 GAATTC G^AATTC
444 GAATTC
HincII 2086 GTCAAC GT[CT]^[GA]AC
2584 GTCAAC
HindII 2086 GTCAAC GT[CT]^[GA]AC
2584 GTCAAC
KasI 1698 GGCGCC G^GCGCC
2022 GGCGCC
MslI 1113 CATCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2211 CACTCTGGTG
MspA1I 337 CCGCTG C[AC]G^C[GT]G
2595 CAGCTG
NarI 1698 GGCGCC GG^CGCC
2022 GGCGCC
NruI 273 TCGCGA TCG^CGA
1242 TCGCGA
PpuMI 149 AGGACCT [GA]G^G[AT]CC[CT]
215 GGGACCT
SfoI 1698 GGCGCC GGC^GCC
2022 GGCGCC
SspBI 1877 TGTACA T^GTACA
2150 TGTACA
SspI 417 AATATT AAT^ATT
593 AATATT
TfiI 430 GAATC G^A[AT]TC
886 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AspHI
AvaI AvaII BanI BanII BbvI
BclI BcnI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BssSI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII