(Circular) (Six-base) MAPSORT of: pFN21AB3294.seq Check: 4414 from: 1 to: 7555 pFN21AB3294 7555 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3145 3649 3145 Size: 504 7051 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5365 278 Size: 53 83 186 936 3829 2468 Fragments arranged by size: 3829 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2742 4898 1937 Size: 805 2156 4594 Fragments arranged by size: 4594 2156 805 Acc65I G'GTAC_C Cuts at: 4876 4876 Size: 7555 AclI AA'CG_TT Cuts at: 1563 5682 6055 1563 Size: 4119 373 3063 Fragments arranged by size: 4119 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2386 4303 5618 7002 7270 1632 Size: 754 1917 1315 1384 268 1917 Fragments arranged by size: 1917 1917 1384 1315 754 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7358 AflIII A'CryG_T Cuts at: 1203 3525 3816 4095 4347 6454 7431 1203 Size: 2322 291 279 252 2107 977 1327 Fragments arranged by size: 2322 2107 1327 977 291 279 252 AgeI A'CCGG_T Cuts at: 6386 6386 Size: 7555 AhdI GACnn_n'nnGTC Cuts at: 6284 6284 Size: 7555 AleI CACnn'nnGTG Cuts at: 1166 4220 1166 Size: 3054 4501 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7555 AlwNI CAG_nnn'CTG Cuts at: 2158 3571 5298 6870 2158 Size: 1413 1727 1572 2843 Fragments arranged by size: 2843 1727 1572 1413 ApaI G_GGCC'C Cuts at: 4070 4295 4070 Size: 225 7330 ApaLI G'TGCA_C Cuts at: 3533 4463 5612 6768 3533 Size: 930 1149 1156 4320 Fragments arranged by size: 4320 1156 1149 930 ApoI r'AATT_y Cuts at: 1493 2263 2449 2879 2955 4378 4664 4864 5090 Size: 770 186 430 76 1423 286 200 226 Cuts at: 5090 5242 7351 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1423 770 430 286 226 200 186 152 76 AseI AT'TA_AT Cuts at: 160 1032 6109 160 Size: 872 5077 1606 Fragments arranged by size: 5077 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7555 AvaI C'yCGr_G Cuts at: 1870 1943 2037 2524 3377 3708 3805 4880 1870 Size: 73 94 487 853 331 97 1075 4545 Fragments arranged by size: 4545 1075 853 487 331 97 94 73 BamHI G'GATC_C Cuts at: 4885 5252 4885 Size: 367 7188 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2129 2182 3143 3703 3745 Size: 325 217 627 342 53 961 560 42 Cuts at: 3745 4027 4876 6331 618 Size: 282 849 1455 1842 Fragments arranged by size: 1842 1455 961 849 627 560 342 325 282 217 53 42 BanII G_rGCy'C Cuts at: 1391 2038 3299 4070 4295 4874 1391 Size: 647 1261 771 225 579 4072 Fragments arranged by size: 4072 1261 771 647 579 225 BbeI G_GCGC'C Cuts at: 3707 4031 3707 Size: 324 7231 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2392 4032 928 Size: 971 493 1640 4451 Fragments arranged by size: 4451 1640 971 493 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3091 3125 5746 5780 1903 Size: 34 1154 34 2621 34 3678 Fragments arranged by size: 3678 2621 1154 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4540 5447 6657 4540 Size: 907 1210 5438 Fragments arranged by size: 5438 1210 907 BclI T'GATC_A Cuts at: 1548 2691 4104 4510 1548 Size: 1143 1413 406 4593 Fragments arranged by size: 4593 1413 1143 406 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6166 136 Size: 107 122 71 631 605 4494 1525 Fragments arranged by size: 4494 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7550 7550 Size: 7555 BlpI GC'TnA_GC Cuts at: 2519 4972 2519 Size: 2453 5102 Bme1580I G_kGCm'C Cuts at: 1790 3537 4070 4295 4467 5616 6772 1790 Size: 1747 533 225 172 1149 1156 2573 Fragments arranged by size: 2573 1747 1156 1149 533 225 172 BmgBI CAC'GTC Cuts at: 1318 3181 3346 4350 1318 Size: 1863 165 1004 4523 Fragments arranged by size: 4523 1863 1004 165 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5286 6244 442 Size: 416 53 83 399 24 3869 958 1753 Fragments arranged by size: 3869 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7555 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2592 2592 Size: 7555 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2186 2249 2342 3030 3608 4213 4557 6215 Size: 947 63 93 688 578 605 344 1658 Cuts at: 6215 1239 Size: 2579 Fragments arranged by size: 2579 1658 947 688 605 578 344 93 63 Bpu10I CC'TnA_GC Cuts at: 2151 2899 2151 Size: 748 6807 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5680 6545 6843 5680 Size: 865 298 6392 Fragments arranged by size: 6392 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4760 6218 882 Size: 617 3261 1458 2219 Fragments arranged by size: 3261 2219 1458 617 BsaAI yAC'GTr Cuts at: 493 1204 3526 5346 493 Size: 711 2322 1820 2702 Fragments arranged by size: 2702 2322 1820 711 BsaBI GATnn'nnATC Cuts at: 7549 7549 Size: 7555 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2204 3704 3798 4028 Size: 53 83 186 936 671 1500 94 230 Cuts at: 4028 4037 4049 5362 5744 6379 275 Size: 9 12 1313 382 635 1451 Fragments arranged by size: 1500 1451 1313 936 671 635 382 230 186 94 83 53 12 9 BsaWI w'CCGG_w Cuts at: 2662 4640 5988 6386 6660 6807 7218 2662 Size: 1978 1348 398 274 147 411 2999 Fragments arranged by size: 2999 1978 1348 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4418 4448 1178 Size: 30 165 30 3015 30 4285 Fragments arranged by size: 4285 3015 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2297 3596 4487 4778 1186 Size: 868 243 1299 891 291 3963 Fragments arranged by size: 3963 1299 891 868 291 243 BseYI C'CCAG_C Cuts at: 6758 7254 6758 Size: 496 7059 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2329 3750 1163 Size: 573 593 1421 4968 Fragments arranged by size: 4968 1421 593 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2278 5005 5766 6794 1540 Size: 275 186 277 2727 761 1028 2301 Fragments arranged by size: 2727 2301 1028 761 277 275 186 BsiHKAI G_wGCw'C Cuts at: 3537 4143 4175 4229 4283 4467 5616 5701 6772 Size: 606 32 54 54 184 1149 85 1071 Cuts at: 6772 3537 Size: 4320 Fragments arranged by size: 4320 1149 1071 606 184 85 54 54 32 BsmI GAATG_Cn' Cuts at: 4482 5066 5159 4482 Size: 584 93 6878 Fragments arranged by size: 6878 584 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2038 3299 3537 4070 4143 4175 4229 Size: 399 248 1261 238 533 73 32 54 Cuts at: 4229 4283 4295 4467 4874 5616 5701 6772 1391 Size: 54 12 172 407 742 85 1071 2174 Fragments arranged by size: 2174 1261 1071 742 533 407 399 248 238 172 85 73 54 54 32 12 BspEI T'CCGG_A Cuts at: 7218 7218 Size: 7555 BspHI T'CATG_A Cuts at: 5444 7174 5444 Size: 1730 5825 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2338 3145 3649 4910 844 Size: 1494 807 504 1261 3489 Fragments arranged by size: 3489 1494 1261 807 504 BsrBI CCG'CTC Cuts at: 1394 1823 2088 3546 4020 5442 1394 Size: 429 265 1458 474 1422 3507 Fragments arranged by size: 3507 1458 1422 474 429 265 BsrDI GCAATG_nn' Cuts at: 65 1248 6050 6224 65 Size: 1183 4802 174 1396 Fragments arranged by size: 4802 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3691 3841 3973 6199 6386 1700 Size: 110 1881 150 132 2226 187 2869 Fragments arranged by size: 2869 2226 1881 187 150 132 110 BsrGI T'GTAC_A Cuts at: 96 3882 4155 96 Size: 3786 273 3496 Fragments arranged by size: 3786 3496 273 BssHII G'CGCG_C Cuts at: 1925 3508 3510 3612 3787 3855 3857 1925 Size: 1583 2 102 175 68 2 5623 Fragments arranged by size: 5623 1583 175 102 68 2 2 BssSI C'ACGA_G Cuts at: 2862 3578 3906 5615 6627 2862 Size: 716 328 1709 1012 3790 Fragments arranged by size: 3790 1709 1012 716 328 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7555 BstBI TT'CG_AA Cuts at: 6359 6359 Size: 7555 BstEII G'GTnAC_C Cuts at: 1184 3277 1184 Size: 2093 5462 BstXI CCAn_nnnn'nTGG Cuts at: 4135 4237 4135 Size: 102 7453 BstYI r'GATC_y Cuts at: 1972 2041 4384 4679 4885 5252 5637 5654 7106 Size: 69 2343 295 206 367 385 17 1452 Cuts at: 7106 7192 7204 7550 1972 Size: 86 12 346 1977 Fragments arranged by size: 2343 1977 1452 385 367 346 295 206 86 69 17 12 BstZ17I GTA'TAC Cuts at: 2743 2743 Size: 7555 BtgI C'CryG_G Cuts at: 513 1065 2004 3315 3330 3987 513 Size: 552 939 1311 15 657 4081 Fragments arranged by size: 4081 1311 939 657 552 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3094 4093 487 Size: 683 56 1868 999 3949 Fragments arranged by size: 3949 1868 999 683 56 BtsI GCAGTG_nn' Cuts at: 3560 3798 4145 4523 5071 5865 5885 3560 Size: 238 347 378 548 794 20 5230 Fragments arranged by size: 5230 794 548 378 347 238 20 ClaI AT'CG_AT Cuts at: 1554 2274 1554 Size: 720 6835 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3982 4017 529 Size: 35 3418 35 4067 Fragments arranged by size: 4067 3418 35 35 DraI TTT'AAA Cuts at: 4612 4859 4612 Size: 247 7308 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6562 809 Size: 507 5246 1802 Fragments arranged by size: 5246 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2089 2140 3318 Size: 54 1425 50 275 18 259 51 1178 Cuts at: 3318 3339 3990 4021 4520 5002 5891 7520 8 Size: 21 651 31 499 482 889 1629 43 Fragments arranged by size: 1629 1425 1178 889 651 499 482 275 259 54 51 50 43 31 21 18 EagI C'GGCC_G Cuts at: 1537 1812 5002 1537 Size: 275 3190 4090 Fragments arranged by size: 4090 3190 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2508 5485 1353 Size: 1155 2977 3423 Fragments arranged by size: 3423 2977 1155 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2189 6142 6516 6662 387 Size: 1064 138 600 3953 374 146 1280 Fragments arranged by size: 3953 1280 1064 600 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 2249 2342 2386 3030 3608 4213 Size: 393 554 63 93 44 644 578 605 Cuts at: 4213 4303 4557 5618 6215 7002 7270 1239 Size: 90 254 1061 597 787 268 1524 Fragments arranged by size: 1524 1061 787 644 605 597 578 554 393 268 254 93 90 63 44 EcoNI CCTnn'n_nnAGG Cuts at: 2228 3917 4472 2228 Size: 1689 555 5311 Fragments arranged by size: 5311 1689 555 EcoO109I rG'GnC_Cy Cuts at: 2026 2155 2221 4291 4505 2026 Size: 129 66 2070 214 5076 Fragments arranged by size: 5076 2070 214 129 66 EcoRI G'AATT_C Cuts at: 2263 2449 4864 5242 2263 Size: 186 2415 378 4576 Fragments arranged by size: 4576 2415 378 186 FspI TGC'GCA Cuts at: 6061 6061 Size: 7555 HaeII r_GCGC'y Cuts at: 1678 3240 3698 3707 3980 4031 4082 4166 6702 Size: 1562 458 9 273 51 51 84 2536 Cuts at: 6702 1678 Size: 2531 Fragments arranged by size: 2536 2531 1562 458 273 84 51 51 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2522 2554 2882 2914 2983 Size: 32 135 32 950 32 328 32 69 Cuts at: 2983 3015 3122 3154 3242 3274 3281 3313 3788 Size: 32 107 32 88 32 7 32 475 Cuts at: 3788 3820 6196 6228 6270 6302 1373 Size: 32 2376 32 42 32 2626 Fragments arranged by size: 2626 2376 950 475 328 135 107 88 69 42 32 32 32 32 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 4093 4591 4899 5145 669 Size: 988 281 2155 498 308 246 3079 Fragments arranged by size: 3079 2155 988 498 308 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7555 HpaI GTT'AAC Cuts at: 5145 5145 Size: 7555 KasI G'GCGC_C Cuts at: 3703 4027 3703 Size: 324 7231 KpnI G_GTAC'C Cuts at: 4880 4880 Size: 7555 MfeI C'AATT_G Cuts at: 5154 5154 Size: 7555 MluI A'CGCG_T Cuts at: 3816 7431 3816 Size: 3615 3940 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3210 6669 6853 1305 Size: 1905 3459 184 2007 Fragments arranged by size: 3459 2007 1905 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4522 10 Size: 54 1425 343 2690 3043 Fragments arranged by size: 3043 2690 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3122 4220 5513 5872 6031 518 Size: 648 1956 1098 1293 359 159 2042 Fragments arranged by size: 2042 1956 1293 1098 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2344 4602 4971 5648 6796 7041 1606 Size: 738 2258 369 677 1148 245 2120 Fragments arranged by size: 2258 2120 1148 738 677 369 245 NaeI GCC'GGC Cuts at: 3693 3843 3975 3693 Size: 150 132 7273 Fragments arranged by size: 7273 150 132 NarI GG'CG_CC Cuts at: 3704 4028 3704 Size: 324 7231 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6064 Fragments arranged by size: 6064 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7555 NgoMIV G'CCGG_C Cuts at: 3691 3841 3973 3691 Size: 150 132 7273 Fragments arranged by size: 7273 150 132 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7555 NotI GC'GGCC_GC Cuts at: 5002 5002 Size: 7555 NruI TCG'CGA Cuts at: 2280 3249 2280 Size: 969 6586 NspI r_CATG'y Cuts at: 1132 1229 4453 4913 6458 1132 Size: 97 3224 460 1545 2229 Fragments arranged by size: 3224 2229 1545 460 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7555 PciI A'CATG_T Cuts at: 6454 6454 Size: 7555 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7555 PfoI T'CCnGG_A Cuts at: 2109 3912 4498 2109 Size: 1803 586 5166 Fragments arranged by size: 5166 1803 586 PmeI GTTT'AAAC Cuts at: 4859 4859 Size: 7555 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5624 5656 7137 7169 5624 Size: 32 1481 32 6010 Fragments arranged by size: 6010 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2155 2221 2155 Size: 66 7489 PshAI GACnn'nnGTC Cuts at: 1613 3908 1613 Size: 2295 5260 PsiI TTA'TAA Cuts at: 5125 5125 Size: 7555 PspOMI G'GGCC_C Cuts at: 4066 4291 4066 Size: 225 7330 PspXI vC'TCGA_Gb Cuts at: 3708 3708 Size: 7555 PstI C_TGCA'G Cuts at: 830 2244 2349 3567 3600 3660 3840 4907 830 Size: 1414 105 1218 33 60 180 1067 3478 Fragments arranged by size: 3478 1414 1218 1067 180 105 60 33 PvuI CG_AT'CG Cuts at: 2001 2278 2001 Size: 277 7278 PvuII CAG'CTG Cuts at: 4602 4602 Size: 7555 SalI G'TCGA_C Cuts at: 1936 4897 1936 Size: 2961 4594 SapI GCTCTTCn'nnn_ Cuts at: 2508 2508 Size: 7555 SbfI CC_TGCA'GG Cuts at: 4907 4907 Size: 7555 ScaI AGT'ACT Cuts at: 5803 7515 5803 Size: 1712 5843 SexAI A'CCwGG_T Cuts at: 4851 4851 Size: 7555 SfcI C'TryA_G Cuts at: 826 1045 2240 2345 2766 3563 3596 3656 3836 Size: 219 1195 105 421 797 33 60 180 Cuts at: 3836 4903 6038 6719 6910 826 Size: 1067 1135 681 191 1471 Fragments arranged by size: 1471 1195 1135 1067 797 681 421 219 191 180 105 60 33 SfoI GGC'GCC Cuts at: 3705 4029 3705 Size: 324 7231 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7555 SgrAI Cr'CCGG_yG Cuts at: 1700 3691 1700 Size: 1991 5564 SmaI CCC'GGG Cuts at: 1872 2039 4882 1872 Size: 167 2843 4545 Fragments arranged by size: 4545 2843 167 SmlI C'TyrA_G Cuts at: 820 1017 1943 3708 5659 6560 6822 820 Size: 197 926 1765 1951 901 262 1553 Fragments arranged by size: 1951 1765 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5346 493 Size: 4853 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7555 SphI G_CATG'C Cuts at: 1132 4453 4913 1132 Size: 3321 460 3774 Fragments arranged by size: 3774 3321 460 SspI AAT'ATT Cuts at: 5 52 2424 2600 5479 5 Size: 47 2372 176 2879 2081 Fragments arranged by size: 2879 2372 2081 176 47 StuI AGG'CCT Cuts at: 1517 4329 1517 Size: 2812 4743 StyI C'CwwG_G Cuts at: 513 1065 2004 4994 513 Size: 552 939 2990 3074 Fragments arranged by size: 3074 2990 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3250 3704 3749 4046 4312 4319 5582 Size: 1255 1670 454 45 297 266 7 1263 Cuts at: 5582 5599 5752 5937 6382 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1670 1498 1263 1255 454 445 297 266 185 153 45 17 7 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3381 3882 4155 Size: 274 80 33 51 1443 1404 501 273 Cuts at: 4155 5801 7513 96 Size: 1646 1712 138 Fragments arranged by size: 1712 1646 1443 1404 501 274 273 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2769 2801 7515 7547 2769 Size: 32 4714 32 2777 Fragments arranged by size: 4714 2777 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7555 XbaI T'CTAG_A Cuts at: 2438 4891 2438 Size: 2453 5102 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3328 3328 Size: 7555 XhoI C'TCGA_G Cuts at: 1943 3708 1943 Size: 1765 5790 XmaI C'CCGG_G Cuts at: 1870 2037 4880 1870 Size: 167 2843 4545 Fragments arranged by size: 4545 2843 167 XmnI GAAnn'nnTTC Cuts at: 5684 5684 Size: 7555 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5363 276 Size: 53 83 186 936 3829 2468 Fragments arranged by size: 3829 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NsiI PacI PmlI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI