Restriction map for pFN21AB3283
 (Circular) (Six-base) MAPSORT of: pFN21AB3283.seq  Check: 7079  from: 1  to: 8272



pFN21AB3283  8272 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2495    2721    3684    5505    2495 
   Size:        226     963    1821    5262

  Fragments arranged by size: 

                5262    1821     963     226
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    6082     278 
   Size:         53      83     186     936    4546    2468

  Fragments arranged by size: 

                4546    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2565    5615    1937 
   Size:        628    3050    4594

  Fragments arranged by size: 

                4594    3050     628
 
Acc65I G'GTAC_C

Cuts at:   2913    5593    2913 
   Size:       2680    5592
 
AclI AA'CG_TT

Cuts at:   1563    6399    6772    1563 
   Size:       4836     373    3063

  Fragments arranged by size: 

                4836    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2048    2584    3161    3434    6335    7719    7987    1632 
   Size:        416     536     577     273    2901    1384     268    1917

  Fragments arranged by size: 

                2901    1917    1384     577     536     416     273     268
            
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    8075
 
AflIII A'CryG_T

Cuts at:   1203    5169    7171    8148    1203 
   Size:       3966    2002     977    1327

  Fragments arranged by size: 

                3966    2002    1327     977
 
AgeI A'CCGG_T

Cuts at:   7103    7103 
   Size:       8272
 
AhdI GACnn_n'nnGTC

Cuts at:   7001    7001 
   Size:       8272
 
AleI CACnn'nnGTG

Cuts at:   1166    2941    1166 
   Size:       1775    6497
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    4049    5072    1184 
   Size:       2865    1023    4384

  Fragments arranged by size: 

                4384    2865    1023
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4413    4445    4413 
   Size:         32    8240
 
AlwNI CAG_nnn'CTG

Cuts at:   2822    3114    3317    3896    4705    5392    6015    7587    2822 
   Size:        292     203     579     809     687     623    1572    3507

  Fragments arranged by size: 

                3507    1572     809     687     623     579     292     203
            
 
ApaI G_GGCC'C

Cuts at:   2210    2246    3235    3745    4105    4260    2210 
   Size:         36     989     510     360     155    6222

  Fragments arranged by size: 

                6222     989     510     360     155      36
 
ApaLI G'TGCA_C

Cuts at:   3946    6329    7485    3946 
   Size:       2383    1156    4733

  Fragments arranged by size: 

                4733    2383    1156
 
ApoI r'AATT_y

Cuts at:   1493    5581    5807    5959    8068    1493 
   Size:       4088     226     152    2109    1697

  Fragments arranged by size: 

                4088    2109    1697     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    6826     160 
   Size:        872    5794    1606

  Fragments arranged by size: 

                5794    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       8272
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2100    3254    3385    3442    4092    4106    4468 
   Size:         73     157    1154     131      57     650      14     362

Cuts at:   4468    5597    1870 
   Size:       1129    4545

  Fragments arranged by size: 

                4545    1154    1129     650     362     157     131      73
                  57      14
 
BamHI G'GATC_C

Cuts at:   2632    4632    5602    5969    2632 
   Size:       2000     970     367    4935

  Fragments arranged by size: 

                4935    2000     970     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2033    2479    2709    2913    2943 
   Size:        325     217     627     246     446     230     204      30

Cuts at:   2943    3056    5085    5237    5252    5292    5359    5593    7048 
   Size:        113    2029     152      15      40      67     234    1455

Cuts at:   7048     618 
   Size:       1842

  Fragments arranged by size: 

                2029    1842    1455     627     446     325     246     234
                 230     217     204     152     113      67      40      30
                  15
 
BanII G_rGCy'C

Cuts at:   1391    2088    2210    2246    2470    2664    3235    3745    4105 
   Size:        697     122      36     224     194     571     510     360

Cuts at:   4105    4260    4557    4712    4805    4962    4991    5133    5374 
   Size:        155     297     155      93     157      29     142     241

Cuts at:   5374    5591    1391 
   Size:        217    4072

  Fragments arranged by size: 

                4072     697     571     510     360     297     241     224
                 217     194     157     155     155     142     122      93
                  36      29
 
BbeI G_GCGC'C

Cuts at:   5241    5256    5296    5241 
   Size:         15      40    8217

  Fragments arranged by size: 

                8217      40      15
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2057    3152    3600     928 
   Size:        971     158    1095     448    5600

  Fragments arranged by size: 

                5600    1095     971     448     158
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3646    3680    6463    6497    1903 
   Size:         34    1709      34    2783      34    3678

  Fragments arranged by size: 

                3678    2783    1709      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   6164    7374    6164 
   Size:       1210    7062
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       8272
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2729    3042    5083 
   Size:        107     122      71     631     605    1057     313    2041

Cuts at:   5083    6883     136 
   Size:       1800    1525

  Fragments arranged by size: 

                2041    1800    1525    1057     631     605     313     122
                 107      71
 
BglII A'GATC_T

Cuts at:   8267    8267 
   Size:       8272
 
BlpI GC'TnA_GC

Cuts at:   3653    4022    4706    5454    5689    3653 
   Size:        369     684     748     235    6236

  Fragments arranged by size: 

                6236     748     684     369     235
 
Bme1580I G_kGCm'C

Cuts at:   1790    2036    2210    2246    2351    2484    2592    2712    2829 
   Size:        246     174      36     105     133     108     120     117

Cuts at:   2829    2948    3235    3508    3534    3745    3950    3966    4071 
   Size:        119     287     273      26     211     205      16     105

Cuts at:   4071    4105    4260    4684    5095    5182    5225    6333    7489 
   Size:         34     155     424     411      87      43    1108    1156

Cuts at:   7489    1790 
   Size:       2573

  Fragments arranged by size: 

                2573    1156    1108     424     411     287     273     246
                 211     205     174     155     133     120     119     117
                 108     105     105      87      43      36      34      26
                  16
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       8272
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3906    3985    4362 
   Size:        416      53      83     399      24    2489      79     377

Cuts at:   4362    5527    6003    6961     442 
   Size:       1165     476     958    1753

  Fragments arranged by size: 

                2489    1753    1165     958     476     416     399     377
                  83      79      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       8272
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3582    4783    3582 
   Size:       1201    7071
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2465    2480    3068    3419    3537    3595    3985    4488 
   Size:       1226      15     588     351     118      58     390     503

Cuts at:   4488    4621    4976    5195    5264    5318    6932    1239 
   Size:        133     355     219      69      54    1614    2579

  Fragments arranged by size: 

                2579    1614    1226     588     503     390     355     351
                 219     133     118      69      58      54      15
 
Bpu10I CC'TnA_GC

Cuts at:   2165    2376    2605    2955    4776    2165 
   Size:        211     229     350    1821    5661

  Fragments arranged by size: 

                5661    1821     350     229     211
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2381    6397    7262    7560    2381 
   Size:       4016     865     298    3093

  Fragments arranged by size: 

                4016    3093     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2441    4202    4220    5142    5429    6935     882 
   Size:        617     942    1761      18     922     287    1506    2219

  Fragments arranged by size: 

                2219    1761    1506     942     922     617     287      18
            
 
BsaAI yAC'GTr

Cuts at:    493    1204    5170    6063     493 
   Size:        711    3966     893    2702

  Fragments arranged by size: 

                3966    2702     893     711
 
BsaBI GATnn'nnATC

Cuts at:   8266    8266 
   Size:       8272
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2160    5187    5238    5253 
   Size:         53      83     186     936     627    3027      51      15

Cuts at:   5253    5293    6079    6461    7096     275 
   Size:         40     786     382     635    1451

  Fragments arranged by size: 

                3027    1451     936     786     635     627     382     186
                  83      53      51      40      15
 
BsaWI w'CCGG_w

Cuts at:   3298    4882    6705    7103    7377    7524    7935    3298 
   Size:       1584    1823     398     274     147     411    3635

  Fragments arranged by size: 

                3635    1823    1584     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    4680    4710    5337    5367    5411 
   Size:         30     165      30    3277      30     627      30      44

Cuts at:   5411    5441    1178 
   Size:         30    4009

  Fragments arranged by size: 

                4009    3277     627     165      44      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3221    3540    3956    4220    5318    1186 
   Size:       2035     319     416     264    1098    4140

  Fragments arranged by size: 

                4140    2035    1098     416     319     264
 
BseYI C'CCAG_C

Cuts at:   2018    2275    2888    2920    2970    3077    3122    3332    3744 
   Size:        257     613      32      50     107      45     210     412

Cuts at:   3744    4231    4557    4725    4730    4962    5190    5474    7475 
   Size:        487     326     168       5     232     228     284    2001

Cuts at:   7475    7971    2018 
   Size:        496    2319

  Fragments arranged by size: 

                2319    2001     613     496     487     412     326     284
                 257     232     228     210     168     107      50      45
                  32       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2096    2732    4718    4802    5405    1163 
   Size:        573     360     636    1986      84     603    4030

  Fragments arranged by size: 

                4030    1986     636     603     573     360      84
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    5229    5722    6483    7511    1540 
   Size:        275     186    3228     493     761    1028    2301

  Fragments arranged by size: 

                3228    2301    1028     761     493     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2171    2470    3950    4028    4599    5402    6333    6418    7489 
   Size:        299    1480      78     571     803     931      85    1071

Cuts at:   7489    2171 
   Size:       2954

  Fragments arranged by size: 

                2954    1480    1071     931     803     571     299      85
                  78
 
BsmI GAATG_Cn'

Cuts at:   2330    2640    3789    5783    5876    2330 
   Size:        310    1149    1994      93    4726

  Fragments arranged by size: 

                4726    1994    1149     310      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2036    2088    2171    2210    2246    2351    2470 
   Size:        399     246      52      83      39      36     105     119

Cuts at:   2470    2484    2592    2664    2712    2829    2948    3235    3508 
   Size:         14     108      72      48     117     119     287     273

Cuts at:   3508    3534    3745    3950    3966    4028    4071    4105    4260 
   Size:         26     211     205      16      62      43      34     155

Cuts at:   4260    4557    4599    4684    4712    4805    4962    4991    5095 
   Size:        297      42      85      28      93     157      29     104

Cuts at:   5095    5133    5182    5225    5374    5402    5591    6333    6418 
   Size:         38      49      43     149      28     189     742      85

Cuts at:   6418    7489    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     297     287     273     246
                 211     205     189     157     155     149     119     119
                 117     108     105     104      93      85      85      83
                  72      62      52      49      48      43      43      42
                  39      38      36      34      29      28      28      26
                  16      14
 
BspEI T'CCGG_A

Cuts at:   4882    7935    4882 
   Size:       3053    5219
 
BspHI T'CATG_A

Cuts at:   6161    7891    6161 
   Size:       1730    6542
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2495    2721    3684    5505    5627     844 
   Size:       1651     226     963    1821     122    3489

  Fragments arranged by size: 

                3489    1821    1651     963     226     122
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2800    3724    6159    1394 
   Size:        429     977     924    2435    3507

  Fragments arranged by size: 

                3507    2435     977     924     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3276    4038    4177    4643    6767    6941      65 
   Size:       1183    2028     762     139     466    2124     174    1396

  Fragments arranged by size: 

                2124    2028    1396    1183     762     466     174     139
            
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2778    3214    3648    3861    6916    7103    1700 
   Size:        110     968     436     434     213    3055     187    2869

  Fragments arranged by size: 

                3055    2869     968     436     434     213     187     110
            
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       8272
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       8272
 
BssSI C'ACGA_G

Cuts at:   6332    7344    6332 
   Size:       1012    7260
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3349    3810     794 
   Size:       2555     461    5256

  Fragments arranged by size: 

                5256    2555     461
 
BstBI TT'CG_AA

Cuts at:   7076    7076 
   Size:       8272
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       8272
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2181    2458    2538    2759    3515    4393    4738    4852    2181 
   Size:        277      80     221     756     878     345     114    5601

  Fragments arranged by size: 

                5601     878     756     345     277     221     114      80
            
 
BstYI r'GATC_y

Cuts at:   1972    2454    2632    3013    4428    4632    5602    5969    6354 
   Size:        482     178     381    1415     204     970     367     385

Cuts at:   6354    6371    7823    7909    7921    8267    1972 
   Size:         17    1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1452    1415     970     482     385     381     367
                 346     204     178      86      17      12
 
BstZ17I GTA'TAC

Cuts at:   2566    2566 
   Size:       8272
 
Bsu36I CC'TnA_GG

Cuts at:   3198    3198 
   Size:       8272
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2116    3817    3936    4299    4900    5547 
   Size:        552     939     112    1701     119     363     601     647

Cuts at:   5547     513 
   Size:       3238

  Fragments arranged by size: 

                3238    1701     939     647     601     552     363     119
                 112
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2913    3856     487 
   Size:        683      56    1687     943    4903

  Fragments arranged by size: 

                4903    1687     943     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2428    2744    3315    4026    4069    5072    5541    5788    6582 
   Size:        316     571     711      43    1003     469     247     794

Cuts at:   6582    6602    2428 
   Size:         20    4098

  Fragments arranged by size: 

                4098    1003     794     711     571     469     316     247
                  43      20
 
ClaI AT'CG_AT

Cuts at:   1554    3362    1554 
   Size:       1808    6464
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    8237
 
DraI TTT'AAA

Cuts at:   5576    5576 
   Size:       8272
 
DraIII CAC_nnn'GTG

Cuts at:   5163    5383    5163 
   Size:        220    8052
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    7279     809 
   Size:        507    5963    1802

  Fragments arranged by size: 

                5963    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2389    2776    3293 
   Size:         54    1425      50     275      18     559     387     517

Cuts at:   3293    3421    4249    5034    5226    5544    5719    6608    8237 
   Size:        128     828     785     192     318     175     889    1629

Cuts at:   8237       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     828     785     559     517     387
                 318     275     192     175     128      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    5226    5719    1537 
   Size:        275    3414     493    4090

  Fragments arranged by size: 

                4090    3414     493     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2807    3830    4592    6202    1353 
   Size:       1454    1023     762    1610    3423

  Fragments arranged by size: 

                3423    1610    1454    1023     762
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    5189    6859    7233    7379     387 
   Size:       1064     138    3600    1670     374     146    1280

  Fragments arranged by size: 

                3600    1670    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2468    2468 
   Size:       8272
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2048    2465    2480    2584    3068    3161    3419 
   Size:        393     416     417      15     104     484      93     258

Cuts at:   3419    3434    3537    3595    3985    4488    4621    4976    5195 
   Size:         15     103      58     390     503     133     355     219

Cuts at:   5195    5264    5318    6335    6932    7719    7987    1239 
   Size:         69      54    1017     597     787     268    1524

  Fragments arranged by size: 

                1524    1017     787     597     503     484     417     416
                 393     390     355     268     258     219     133     104
                 103      93      69      58      54      15      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2152    3309    3570    4527    4589    5300    5333    2152 
   Size:       1157     261     957      62     711      33    5091

  Fragments arranged by size: 

                5091    1157     957     711     261      62      33
 
EcoO109I rG'GnC_Cy

Cuts at:   2206    2207    2242    2243    3043    3111    3232    3410    3439 
   Size:          1      35       1     800      68     121     178      29

Cuts at:   3439    3730    4102    4256    4257    4483    5338    5389    5471 
   Size:        291     372     154       1     226     855      51      82

Cuts at:   5471    2206 
   Size:       5007

  Fragments arranged by size: 

                5007     855     800     372     291     226     178     154
                 121      82      68      51      35      29       1       1
                   1
 
EcoRI G'AATT_C

Cuts at:   5581    5959    5581 
   Size:        378    7894
 
EcoRV GAT'ATC

Cuts at:   4060    4060 
   Size:       8272
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2680    5123    2680 
   Size:       2443    5829
 
FspI TGC'GCA

Cuts at:   4478    6778    4478 
   Size:       2300    5972
 
HaeII r_GCGC'y

Cuts at:   1678    4706    5241    5256    5296    7419    1678 
   Size:       3028     535      15      40    2123    2531

  Fragments arranged by size: 

                3028    2531    2123     535      40      15
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3368    3400    4657    4689    4787 
   Size:         32     135      32    1796      32    1257      32      98

Cuts at:   4787    4819    6913    6945    6987    7019    1373 
   Size:         32    2094      32      42      32    2626

  Fragments arranged by size: 

                2626    2094    1796    1257     135      98      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2269    5616    5862     669 
   Size:        988     281     331    3347     246    3079

  Fragments arranged by size: 

                3347    3079     988     331     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       8272
 
HpaI GTT'AAC

Cuts at:   5862    5862 
   Size:       8272
 
KasI G'GCGC_C

Cuts at:   5237    5252    5292    5237 
   Size:         15      40    8217

  Fragments arranged by size: 

                8217      40      15
 
KpnI G_GTAC'C

Cuts at:   2917    5597    2917 
   Size:       2680    5592
 
MfeI C'AATT_G

Cuts at:   5871    5871 
   Size:       8272
 
MluI A'CGCG_T

Cuts at:   8148    8148 
   Size:       8272
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    7386    7570    1305 
   Size:       6081     184    2007

  Fragments arranged by size: 

                6081    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2391    3423      10 
   Size:         54    1425     343     559    1032    4859

  Fragments arranged by size: 

                4859    1425    1032     559     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2385    2532    2941    4031    5054    6230    6589 
   Size:        648    1219     147     409    1090    1023    1176     359

Cuts at:   6589    6748     518 
   Size:        159    2042

  Fragments arranged by size: 

                2042    1219    1176    1090    1023     648     409     359
                 159     147
 
MspA1I CmG'CkG

Cuts at:   1606    2125    2326    2672    2920    2974    3515    3748    3852 
   Size:        519     201     346     248      54     541     233     104

Cuts at:   3852    3938    4223    4301    5265    5688    6365    7513    7758 
   Size:         86     285      78     964     423     677    1148     245

Cuts at:   7758    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1148     964     677     541     519     423     346
                 285     248     245     233     201     104      86      78
                  54
 
NaeI GCC'GGC

Cuts at:   2780    3650    2780 
   Size:        870    7402
 
NarI GG'CG_CC

Cuts at:   5238    5253    5293    5238 
   Size:         15      40    8217

  Fragments arranged by size: 

                8217      40      15
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2116    3817    4900     513 
   Size:        552     939     112    1701    1083    3885

  Fragments arranged by size: 

                3885    1701    1083     939     552     112
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       8272
 
NgoMIV G'CCGG_C

Cuts at:   2778    3648    2778 
   Size:        870    7402
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       8272
 
NotI GC'GGCC_GC

Cuts at:   5719    5719 
   Size:       8272
 
NspI r_CATG'y

Cuts at:   1132    1229    2221    5063    5630    7175    1132 
   Size:         97     992    2842     567    1545    2229

  Fragments arranged by size: 

                2842    2229    1545     992     567      97
 
PasI CC'CwG_GG

Cuts at:   1348    2203    2559    3108    3629    4444    4529    1348 
   Size:        855     356     549     521     815      85    5091

  Fragments arranged by size: 

                5091     855     815     549     521     356      85
 
PciI A'CATG_T

Cuts at:   7171    7171 
   Size:       8272
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2326    2900    4012    1108 
   Size:       1218     574    1112    5368

  Fragments arranged by size: 

                5368    1218    1112     574
 
PfoI T'CCnGG_A

Cuts at:   2457    3435    4392    4431    4590    2457 
   Size:        978     957      39     159    6139

  Fragments arranged by size: 

                6139     978     957     159      39
 
PmeI GTTT'AAAC

Cuts at:   5576    5576 
   Size:       8272
 
PmlI CAC'GTG

Cuts at:   5170    5170 
   Size:       8272
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3083    3115    6341    6373    7854    7886    3083 
   Size:         32    3226      32    1481      32    3469

  Fragments arranged by size: 

                3469    3226    1481      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   3410    3439    5471    3410 
   Size:         29    2032    6211

  Fragments arranged by size: 

                6211    2032      29
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       8272
 
PsiI TTA'TAA

Cuts at:   5842    5842 
   Size:       8272
 
PspOMI G'GGCC_C

Cuts at:   2206    2242    3231    3741    4101    4256    2206 
   Size:         36     989     510     360     155    6222

  Fragments arranged by size: 

                6222     989     510     360     155      36
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   2897    2929    2897 
   Size:         32    8240
 
PstI C_TGCA'G

Cuts at:    830    2718    3322    3585    3806    3857    4551    5368    5624 
   Size:       1888     604     263     221      51     694     817     256

Cuts at:   5624     830 
   Size:       3478

  Fragments arranged by size: 

                3478    1888     817     694     604     263     256     221
                  51
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       8272
 
PvuII CAG'CTG

Cuts at:   2672    2920    3515    5265    2672 
   Size:        248     595    1750    5679

  Fragments arranged by size: 

                5679    1750     595     248
 
SacI G_AGCT'C

Cuts at:   2470    2470 
   Size:       8272
 
SacII CC_GC'GG

Cuts at:   3939    4302    3939 
   Size:        363    7909
 
SalI G'TCGA_C

Cuts at:   1936    5614    1936 
   Size:       3678    4594
 
SanDI GG'GwC_CC

Cuts at:   5471    5471 
   Size:       8272
 
SapI GCTCTTCn'nnn_

Cuts at:   2807    2807 
   Size:       8272
 
SbfI CC_TGCA'GG

Cuts at:   5368    5624    5368 
   Size:        256    8016
 
ScaI AGT'ACT

Cuts at:   4889    6520    8232    4889 
   Size:       1631    1712    4929

  Fragments arranged by size: 

                4929    1712    1631
 
SexAI A'CCwGG_T

Cuts at:   4038    5040    5379    4038 
   Size:       1002     339    6931

  Fragments arranged by size: 

                6931    1002     339
 
SfcI C'TryA_G

Cuts at:    826    1045    2714    3318    3581    3802    3853    4547    5364 
   Size:        219    1669     604     263     221      51     694     817

Cuts at:   5364    5620    6755    7436    7627     826 
   Size:        256    1135     681     191    1471

  Fragments arranged by size: 

                1669    1471    1135     817     694     681     604     263
                 256     221     219     191      51
 
SfoI GGC'GCC

Cuts at:   5239    5254    5294    5239 
   Size:         15      40    8217

  Fragments arranged by size: 

                8217      40      15
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       8272
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       8272
 
SmaI CCC'GGG

Cuts at:   1872    3444    4094    4108    4470    5599    1872 
   Size:       1572     650      14     362    1129    4545

  Fragments arranged by size: 

                4545    1572    1129     650     362      14
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2100    2396    6376    7277    7539     820 
   Size:        197     926     157     296    3980     901     262    1553

  Fragments arranged by size: 

                3980    1553     926     901     296     262     197     157
            
 
SnaBI TAC'GTA

Cuts at:    493    6063     493 
   Size:       5570    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       8272
 
SphI G_CATG'C

Cuts at:   1132    5063    5630    1132 
   Size:       3931     567    3774

  Fragments arranged by size: 

                3931    3774     567
 
SspI AAT'ATT

Cuts at:      5      52    6196       5 
   Size:         47    6144    2081

  Fragments arranged by size: 

                6144    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2859    3098    3663    5115    1517 
   Size:       1342     239     565    1452    4674

  Fragments arranged by size: 

                4674    1452    1342     565     239
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2116    2211    2664    2908    2939    3757 
   Size:        552     939     112      95     453     244      31     818

Cuts at:   3757    3817    4900    5711     513 
   Size:         60    1083     811    3074

  Fragments arranged by size: 

                3074    1083     939     818     811     552     453     244
                 112      95      60      31
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2467    2984    3013    3711    3848    4196    6299 
   Size:       1255     887     517      29     698     137     348    2103

Cuts at:   6299    6316    6469    6654    7099     325 
   Size:         17     153     185     445    1498

  Fragments arranged by size: 

                2103    1498    1255     887     698     517     445     348
                 185     153     137      29      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4887    6518    8230 
   Size:        274      80      33      51    1443    2910    1631    1712

Cuts at:   8230      96 
   Size:        138

  Fragments arranged by size: 

                2910    1712    1631    1443     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3686    3718    4680    4712    5337    5369    5411    5443    8232 
   Size:         32     962      32     625      32      42      32    2789

Cuts at:   8232    8264    3686 
   Size:         32    3694

  Fragments arranged by size: 

                3694    2789     962     625      42      32      32      32
                  32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    5042    1316 
   Size:       3726    4546
 
XbaI T'CTAG_A

Cuts at:   5608    5608 
   Size:       8272
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   4009    5079    4009 
   Size:       1070    7202
 
XhoI C'TCGA_G

Cuts at:   1943    2100    1943 
   Size:        157    8115
 
XmaI C'CCGG_G

Cuts at:   1870    3442    4092    4106    4468    5597    1870 
   Size:       1572     650      14     362    1129    4545

  Fragments arranged by size: 

                4545    1572    1129     650     362      14
 
XmnI GAAnn'nnTTC

Cuts at:   5102    6401    5102 
   Size:       1299    6973
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    6080     276 
   Size:         53      83     186     936    4546    2468

  Fragments arranged by size: 

                4546    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
     PsrI     PstI     PvuI    PvuII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AscI    AvrII     BaeI    BbvCI    BfrBI    BsiWI    BsmBI
     FseI    FspAI     NruI     NsiI     PacI    PspXI    RsrII     SfiI
     SrfI     SwaI
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