(Circular) (Six-base) MAPSORT of: pFN21AB3272.seq Check: 545 from: 1 to: 8500 pFN21AB3272 8500 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2146 3876 2146 Size: 1730 6770 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3159 4269 6310 278 Size: 53 83 186 936 1623 1110 2041 2468 Fragments arranged by size: 2468 2041 1623 1110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4809 4920 4959 5843 1937 Size: 2872 111 39 884 4594 Fragments arranged by size: 4594 2872 884 111 39 Acc65I G'GTAC_C Cuts at: 5821 5821 Size: 8500 AclI AA'CG_TT Cuts at: 1563 6627 7000 1563 Size: 5064 373 3063 Fragments arranged by size: 5064 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3502 3808 4039 4949 5525 6563 7947 8215 Size: 1870 306 231 910 576 1038 1384 268 Cuts at: 8215 1632 Size: 1917 Fragments arranged by size: 1917 1870 1384 1038 910 576 306 268 231 AfeI AGC'GCT Cuts at: 4229 4229 Size: 8500 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8303 AflIII A'CryG_T Cuts at: 1203 2988 3450 7399 8376 1203 Size: 1785 462 3949 977 1327 Fragments arranged by size: 3949 1785 1327 977 462 AgeI A'CCGG_T Cuts at: 7331 7331 Size: 8500 AhdI GACnn_n'nnGTC Cuts at: 7229 7229 Size: 8500 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8500 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8500 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3584 3616 3584 Size: 32 8468 AlwNI CAG_nnn'CTG Cuts at: 2788 2907 6243 7815 2788 Size: 119 3336 1572 3473 Fragments arranged by size: 3473 3336 1572 119 ApaI G_GGCC'C Cuts at: 3096 3383 3096 Size: 287 8213 ApaLI G'TGCA_C Cuts at: 6557 7713 6557 Size: 1156 7344 ApoI r'AATT_y Cuts at: 1493 2663 5373 5809 6035 6187 8296 1493 Size: 1170 2710 436 226 152 2109 1697 Fragments arranged by size: 2710 2109 1697 1170 436 226 152 AseI AT'TA_AT Cuts at: 160 1032 7054 160 Size: 872 6022 1606 Fragments arranged by size: 6022 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8500 AvaI C'yCGr_G Cuts at: 1870 1943 2117 3193 3384 3996 4214 4412 5162 Size: 73 174 1076 191 612 218 198 750 Cuts at: 5162 5825 1870 Size: 663 4545 Fragments arranged by size: 4545 1076 750 663 612 218 198 191 174 73 BamHI G'GATC_C Cuts at: 3765 4285 4446 5830 6197 3765 Size: 520 161 1384 367 6068 Fragments arranged by size: 6068 1384 520 367 161 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3680 4382 5821 7276 618 Size: 325 217 627 1893 702 1439 1455 1842 Fragments arranged by size: 1893 1842 1455 1439 702 627 325 217 BanII G_rGCy'C Cuts at: 1391 2068 2595 2782 3096 3383 4152 4221 4413 Size: 677 527 187 314 287 769 69 192 Cuts at: 4413 4638 4692 4794 5347 5819 1391 Size: 225 54 102 553 472 4072 Fragments arranged by size: 4072 769 677 553 527 472 314 287 225 192 187 102 69 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2286 2510 4533 4615 928 Size: 971 387 224 2023 82 4813 Fragments arranged by size: 4813 2023 971 387 224 82 BbvCI CC'TCA_GC Cuts at: 2321 5330 5612 2321 Size: 3009 282 5209 Fragments arranged by size: 5209 3009 282 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2296 2330 4025 4059 6691 6725 1903 Size: 34 359 34 1695 34 2632 34 3678 Fragments arranged by size: 3678 2632 1695 359 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6392 7602 6392 Size: 1210 7290 BclI T'GATC_A Cuts at: 1548 2139 2520 1548 Size: 591 381 7528 Fragments arranged by size: 7528 591 381 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3387 7111 136 Size: 107 122 71 631 605 1715 3724 1525 Fragments arranged by size: 3724 1715 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5268 8495 5268 Size: 3227 5273 BlpI GC'TnA_GC Cuts at: 2330 2594 2847 4367 5917 2330 Size: 264 253 1520 1550 4913 Fragments arranged by size: 4913 1550 1520 264 253 Bme1580I G_kGCm'C Cuts at: 1790 3096 3383 3558 3912 4387 6561 7717 1790 Size: 1306 287 175 354 475 2174 1156 2573 Fragments arranged by size: 2573 2174 1306 1156 475 354 287 175 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8500 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3197 4284 4771 Size: 416 53 83 399 24 1780 1087 487 Cuts at: 4771 6231 7189 442 Size: 1460 958 1753 Fragments arranged by size: 1780 1753 1460 1087 958 487 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8500 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4501 4501 Size: 8500 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 2185 4244 4652 5594 5714 7160 1239 Size: 851 95 2059 408 942 120 1446 2579 Fragments arranged by size: 2579 2059 1446 942 851 408 120 95 Bpu10I CC'TnA_GC Cuts at: 2321 2781 3956 5330 5612 2321 Size: 460 1175 1374 282 5209 Fragments arranged by size: 5209 1374 1175 460 282 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6625 7490 7788 6625 Size: 865 298 7337 Fragments arranged by size: 7337 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3698 4548 7163 882 Size: 617 2199 850 2615 2219 Fragments arranged by size: 2615 2219 2199 850 617 BsaAI yAC'GTr Cuts at: 493 1204 2880 2991 6291 493 Size: 711 1676 111 3300 2702 Fragments arranged by size: 3300 2702 1676 711 111 BsaBI GATnn'nnATC Cuts at: 3359 8494 3359 Size: 5135 3365 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3156 4266 6307 6689 Size: 53 83 186 936 1623 1110 2041 382 Cuts at: 6689 7324 275 Size: 635 1451 Fragments arranged by size: 2041 1623 1451 1110 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4017 4657 6933 7331 7605 7752 8163 4017 Size: 640 2276 398 274 147 411 4354 Fragments arranged by size: 4354 2276 640 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2957 2987 3584 3614 4385 Size: 30 165 30 1554 30 597 30 771 Cuts at: 4385 4415 1178 Size: 30 5263 Fragments arranged by size: 5263 1554 771 597 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2965 3236 3560 4517 4700 5587 5629 1186 Size: 1779 271 324 957 183 887 42 4057 Fragments arranged by size: 4057 1779 957 887 324 271 183 42 BseYI C'CCAG_C Cuts at: 2074 3084 3335 3376 4114 7703 8199 2074 Size: 1010 251 41 738 3589 496 2375 Fragments arranged by size: 3589 2375 1010 738 496 251 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3182 1163 Size: 573 1446 6481 Fragments arranged by size: 6481 1446 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4102 5950 6711 7739 1540 Size: 275 186 2101 1848 761 1028 2301 Fragments arranged by size: 2301 2101 1848 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2581 2595 3228 4152 4221 4692 4794 5215 6561 Size: 14 633 924 69 471 102 421 1346 Cuts at: 6561 6646 7717 2581 Size: 85 1071 3364 Fragments arranged by size: 3364 1346 1071 924 633 471 421 102 85 69 14 BsmI GAATG_Cn' Cuts at: 2413 4171 6011 6104 2413 Size: 1758 1840 93 4809 Fragments arranged by size: 4809 1840 1758 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2141 3656 3854 4877 2141 Size: 1515 198 1023 5764 Fragments arranged by size: 5764 1515 1023 198 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2068 2581 2595 2782 3096 3228 3383 Size: 399 278 513 14 187 314 132 155 Cuts at: 3383 3558 3912 4152 4221 4387 4413 4638 4692 Size: 175 354 240 69 166 26 225 54 Cuts at: 4692 4794 5215 5347 5819 6561 6646 7717 1391 Size: 102 421 132 472 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 513 472 421 399 354 314 278 240 225 187 175 166 155 132 132 102 85 69 54 26 14 BspEI T'CCGG_A Cuts at: 4017 8163 4017 Size: 4146 4354 BspHI T'CATG_A Cuts at: 6389 8119 6389 Size: 1730 6770 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2146 2739 3294 3876 5855 844 Size: 1302 593 555 582 1979 3489 Fragments arranged by size: 3489 1979 1302 593 582 555 BsrBI CCG'CTC Cuts at: 1394 1823 2122 2806 3771 4872 6387 1394 Size: 429 299 684 965 1101 1515 3507 Fragments arranged by size: 3507 1515 1101 965 684 429 299 BsrDI GCAATG_nn' Cuts at: 65 1248 3495 5683 6995 7169 65 Size: 1183 2247 2188 1312 174 1396 Fragments arranged by size: 2247 2188 1396 1312 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3106 4101 7144 7331 1700 Size: 110 1296 995 3043 187 2869 Fragments arranged by size: 3043 2869 1296 995 187 110 BsrGI T'GTAC_A Cuts at: 96 3447 96 Size: 3351 5149 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8500 BssSI C'ACGA_G Cuts at: 2892 3926 6560 7572 2892 Size: 1034 2634 1012 3820 Fragments arranged by size: 3820 2634 1034 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4111 794 Size: 3317 5183 BstBI TT'CG_AA Cuts at: 7304 7304 Size: 8500 BstEII G'GTnAC_C Cuts at: 1184 3002 1184 Size: 1818 6682 BstYI r'GATC_y Cuts at: 1972 2785 2970 3765 4285 4446 5178 5268 5830 Size: 813 185 795 520 161 732 90 562 Cuts at: 5830 6197 6582 6599 8051 8137 8149 8495 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 813 795 732 562 520 385 367 346 185 161 90 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4537 4596 4537 Size: 59 8441 BtgI C'CryG_G Cuts at: 513 1065 2013 3118 3303 3777 3938 3961 4378 Size: 552 948 1105 185 474 161 23 417 Cuts at: 4378 513 Size: 4635 Fragments arranged by size: 4635 1105 948 552 474 417 185 161 23 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2417 3405 4374 4765 487 Size: 683 56 1191 988 969 391 4222 Fragments arranged by size: 4222 1191 988 969 683 391 56 BtsI GCAGTG_nn' Cuts at: 2545 3226 3757 6016 6810 6830 2545 Size: 681 531 2259 794 20 4215 Fragments arranged by size: 4215 2259 794 681 531 20 ClaI AT'CG_AT Cuts at: 1554 2651 1554 Size: 1097 7403 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8465 DraI TTT'AAA Cuts at: 2530 5804 2530 Size: 3274 5226 DraIII CAC_nnn'GTG Cuts at: 4111 4111 Size: 8500 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4805 7507 809 Size: 507 3489 2702 1802 Fragments arranged by size: 3489 2702 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3255 4099 5564 Size: 54 1425 50 275 18 1425 844 1465 Cuts at: 5564 5947 6836 8465 8 Size: 383 889 1629 43 Fragments arranged by size: 1629 1465 1425 1425 889 844 383 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4099 5947 1537 Size: 275 2287 1848 4090 Fragments arranged by size: 4090 2287 1848 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2042 2621 3614 4990 5577 5765 6430 1353 Size: 689 579 993 1376 587 188 665 3423 Fragments arranged by size: 3423 1376 993 689 665 587 579 188 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2213 4114 4994 7087 7461 7607 Size: 1064 138 624 1901 880 2093 374 146 Cuts at: 7607 387 Size: 1280 Fragments arranged by size: 2093 1901 1280 1064 880 624 374 146 138 EcoICRI GAG'CTC Cuts at: 2593 4150 4219 4690 4792 2593 Size: 1557 69 471 102 6301 Fragments arranged by size: 6301 1557 471 102 69 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2185 3502 3808 4039 4244 4652 Size: 393 458 95 1317 306 231 205 408 Cuts at: 4652 4949 5525 5594 5714 6563 7160 7947 8215 Size: 297 576 69 120 849 597 787 268 Cuts at: 8215 1239 Size: 1524 Fragments arranged by size: 1524 1317 849 787 597 576 458 408 393 306 297 268 231 205 120 95 69 EcoO109I rG'GnC_Cy Cuts at: 3380 3789 4005 4119 4945 3380 Size: 409 216 114 826 6935 Fragments arranged by size: 6935 826 409 216 114 EcoRI G'AATT_C Cuts at: 2663 5809 6187 2663 Size: 3146 378 4976 Fragments arranged by size: 4976 3146 378 EcoRV GAT'ATC Cuts at: 2203 2203 Size: 8500 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2527 3532 4858 5606 2527 Size: 1005 1326 748 5421 Fragments arranged by size: 5421 1326 1005 748 FspI TGC'GCA Cuts at: 3053 5675 7006 3053 Size: 2622 1331 4547 Fragments arranged by size: 4547 2622 1331 HaeII r_GCGC'y Cuts at: 1678 4231 7647 1678 Size: 2553 3416 2531 Fragments arranged by size: 3416 2553 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4238 4270 7141 7173 7215 Size: 32 135 32 2666 32 2871 32 42 Cuts at: 7215 7247 1373 Size: 32 2626 Fragments arranged by size: 2871 2666 2626 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2365 3294 3403 4921 4981 5844 Size: 988 281 427 929 109 1518 60 863 Cuts at: 5844 6090 669 Size: 246 3079 Fragments arranged by size: 3079 1518 988 929 863 427 281 246 109 60 HindIII A'AGCT_T Cuts at: 748 3033 748 Size: 2285 6215 HpaI GTT'AAC Cuts at: 2365 6090 2365 Size: 3725 4775 KpnI G_GTAC'C Cuts at: 5825 5825 Size: 8500 MfeI C'AATT_G Cuts at: 6099 6099 Size: 8500 MluI A'CGCG_T Cuts at: 8376 8376 Size: 8500 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4491 5372 7614 7798 1305 Size: 3186 881 2242 184 2007 Fragments arranged by size: 3186 2242 2007 881 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3257 10 Size: 54 1425 343 1425 5253 Fragments arranged by size: 5253 1425 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2586 3983 5069 6458 6817 6976 518 Size: 648 1420 1397 1086 1389 359 159 2042 Fragments arranged by size: 2042 1420 1397 1389 1086 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2342 3010 3045 3325 3343 3963 5611 5734 Size: 736 668 35 280 18 620 1648 123 Cuts at: 5734 5916 6593 7741 7986 1606 Size: 182 677 1148 245 2120 Fragments arranged by size: 2120 1648 1148 736 677 668 620 280 245 182 123 35 18 NaeI GCC'GGC Cuts at: 4103 4103 Size: 8500 NcoI C'CATG_G Cuts at: 513 1065 3118 3777 513 Size: 552 2053 659 5236 Fragments arranged by size: 5236 2053 659 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8500 NgoMIV G'CCGG_C Cuts at: 4101 4101 Size: 8500 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8500 NotI GC'GGCC_GC Cuts at: 5947 5947 Size: 8500 NspI r_CATG'y Cuts at: 1132 1229 2938 3454 3982 5068 5858 7403 1132 Size: 97 1709 516 528 1086 790 1545 2229 Fragments arranged by size: 2229 1709 1545 1086 790 528 516 97 PasI CC'CwG_GG Cuts at: 1348 2228 1348 Size: 880 7620 PciI A'CATG_T Cuts at: 3450 7399 3450 Size: 3949 4551 PflMI CCAn_nnn'nTGG Cuts at: 1108 3939 1108 Size: 2831 5669 PfoI T'CCnGG_A Cuts at: 4221 4629 5571 4221 Size: 408 942 7150 Fragments arranged by size: 7150 942 408 PmeI GTTT'AAAC Cuts at: 5804 5804 Size: 8500 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3584 3616 6569 6601 8082 8114 3584 Size: 32 2953 32 1481 32 3970 Fragments arranged by size: 3970 2953 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3789 4119 3789 Size: 330 8170 PshAI GACnn'nnGTC Cuts at: 1613 4955 1613 Size: 3342 5158 PsiI TTA'TAA Cuts at: 6070 6070 Size: 8500 PspOMI G'GGCC_C Cuts at: 3092 3379 3092 Size: 287 8213 PspXI vC'TCGA_Gb Cuts at: 3193 3193 Size: 8500 PstI C_TGCA'G Cuts at: 830 3233 3648 4495 5121 5852 830 Size: 2403 415 847 626 731 3478 Fragments arranged by size: 3478 2403 847 731 626 415 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8500 PvuII CAG'CTG Cuts at: 2342 3010 3325 3343 5611 5734 2342 Size: 668 315 18 2268 123 5108 Fragments arranged by size: 5108 2268 668 315 123 18 SacI G_AGCT'C Cuts at: 2595 4152 4221 4692 4794 2595 Size: 1557 69 471 102 6301 Fragments arranged by size: 6301 1557 471 102 69 SacII CC_GC'GG Cuts at: 3964 3964 Size: 8500 SalI G'TCGA_C Cuts at: 1936 4919 5842 1936 Size: 2983 923 4594 Fragments arranged by size: 4594 2983 923 SanDI GG'GwC_CC Cuts at: 3789 3789 Size: 8500 SapI GCTCTTCn'nnn_ Cuts at: 2042 2042 Size: 8500 SbfI CC_TGCA'GG Cuts at: 5852 5852 Size: 8500 ScaI AGT'ACT Cuts at: 4457 4760 6748 8460 4457 Size: 303 1988 1712 4497 Fragments arranged by size: 4497 1988 1712 303 SexAI A'CCwGG_T Cuts at: 4800 4800 Size: 8500 SfcI C'TryA_G Cuts at: 826 1045 3229 3573 3644 4491 5117 5848 6983 Size: 219 2184 344 71 847 626 731 1135 Cuts at: 6983 7664 7855 826 Size: 681 191 1471 Fragments arranged by size: 2184 1471 1135 847 731 681 626 344 219 191 71 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8500 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8500 SmaI CCC'GGG Cuts at: 1872 3998 4414 5827 1872 Size: 2126 416 1413 4545 Fragments arranged by size: 4545 2126 1413 416 SmlI C'TyrA_G Cuts at: 820 1017 1943 3193 6604 7505 7767 820 Size: 197 926 1250 3411 901 262 1553 Fragments arranged by size: 3411 1553 1250 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6291 493 Size: 5798 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8500 SphI G_CATG'C Cuts at: 1132 5858 1132 Size: 4726 3774 SspI AAT'ATT Cuts at: 5 52 4560 6424 5 Size: 47 4508 1864 2081 Fragments arranged by size: 4508 2081 1864 47 StuI AGG'CCT Cuts at: 1517 3263 1517 Size: 1746 6754 StyI C'CwwG_G Cuts at: 513 1065 3118 3258 3777 4404 4740 4833 5939 Size: 552 2053 140 519 627 336 93 1106 Cuts at: 5939 513 Size: 3074 Fragments arranged by size: 3074 2053 1106 627 552 519 336 140 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6527 6544 6697 6882 7327 325 Size: 1255 4947 17 153 185 445 1498 Fragments arranged by size: 4947 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3447 4455 4758 Size: 274 80 33 51 1443 1470 1008 303 Cuts at: 4758 4932 5061 6746 8458 96 Size: 174 129 1685 1712 138 Fragments arranged by size: 1712 1685 1470 1443 1008 303 274 174 138 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4385 4417 5028 5060 8460 8492 4385 Size: 32 611 32 3400 32 4393 Fragments arranged by size: 4393 3400 611 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8500 XbaI T'CTAG_A Cuts at: 5836 5836 Size: 8500 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4678 4678 Size: 8500 XhoI C'TCGA_G Cuts at: 1943 3193 1943 Size: 1250 7250 XmaI C'CCGG_G Cuts at: 1870 3996 4412 5825 1870 Size: 2126 416 1413 4545 Fragments arranged by size: 4545 2126 1413 416 XmnI GAAnn'nnTTC Cuts at: 3599 6629 3599 Size: 3030 5470 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3157 4267 6308 276 Size: 53 83 186 936 1623 1110 2041 2468 Fragments arranged by size: 2468 2041 1623 1110 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I EcoNI FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SfiI SfoI SrfI SwaI