Restriction map for
pFN21AB3272
(Linear) (Six-base) MAPSORT of: KIBB3272.seq Check: 3475 from: 1 to: 3795
KIBB3272.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 141 1871 3795
Size: 141 1730 1924
Fragments arranged by size:
1924 1730 141
AatII G_ACGT'C
Cuts at: 0 1154 2264 3795
Size: 1154 1110 1531
Fragments arranged by size:
1531 1154 1110
AccI GT'mk_AC
Cuts at: 0 2804 2915 2954 3795
Size: 2804 111 39 841
Fragments arranged by size:
2804 841 111 39
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1497 1803 2034 2944 3520 3795
Size: 1497 306 231 910 576 275
Fragments arranged by size:
1497 910 576 306 275 231
AfeI AGC'GCT
Cuts at: 0 2224 3795
Size: 2224 1571
AflIII A'CryG_T
Cuts at: 0 983 1445 3795
Size: 983 462 2350
Fragments arranged by size:
2350 983 462
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1579 1611 3795
Size: 1579 32 2184
Fragments arranged by size:
2184 1579 32
AlwNI CAG_nnn'CTG
Cuts at: 0 783 902 3795
Size: 783 119 2893
Fragments arranged by size:
2893 783 119
ApaI G_GGCC'C
Cuts at: 0 1091 1378 3795
Size: 1091 287 2417
Fragments arranged by size:
2417 1091 287
ApoI r'AATT_y
Cuts at: 0 658 3368 3795
Size: 658 2710 427
Fragments arranged by size:
2710 658 427
AvaI C'yCGr_G
Cuts at: 0 112 1188 1379 1991 2209 2407 3157 3795
Size: 112 1076 191 612 218 198 750 638
Fragments arranged by size:
1076 750 638 612 218 198 191 112
BamHI G'GATC_C
Cuts at: 0 1760 2280 2441 3795
Size: 1760 520 161 1354
Fragments arranged by size:
1760 1354 520 161
BanI G'GyrC_C
Cuts at: 0 1675 2377 3795
Size: 1675 702 1418
Fragments arranged by size:
1675 1418 702
BanII G_rGCy'C
Cuts at: 0 63 590 777 1091 1378 2147 2216 2408
Size: 63 527 187 314 287 769 69 192
Cuts at: 2408 2633 2687 2789 3342 3795
Size: 225 54 102 553 453
Fragments arranged by size:
769 553 527 453 314 287 225 192
187 102 69 63 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 281 505 2528 2610 3795
Size: 281 224 2023 82 1185
Fragments arranged by size:
2023 1185 281 224 82
BbvCI CC'TCA_GC
Cuts at: 0 316 3325 3607 3795
Size: 316 3009 282 188
Fragments arranged by size:
3009 316 282 188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 291 325 2020 2054 3795
Size: 291 34 1695 34 1741
Fragments arranged by size:
1741 1695 291 34 34
BclI T'GATC_A
Cuts at: 0 134 515 3795
Size: 134 381 3280
Fragments arranged by size:
3280 381 134
BglI GCCn_nnn'nGGC
Cuts at: 0 1382 3795
Size: 1382 2413
BglII A'GATC_T
Cuts at: 0 3263 3795
Size: 3263 532
BlpI GC'TnA_GC
Cuts at: 0 325 589 842 2362 3795
Size: 325 264 253 1520 1433
Fragments arranged by size:
1520 1433 325 264 253
Bme1580I G_kGCm'C
Cuts at: 0 1091 1378 1553 1907 2382 3795
Size: 1091 287 175 354 475 1413
Fragments arranged by size:
1413 1091 475 354 287 175
BmrI ACTGGGnnnn_n'
Cuts at: 0 1192 2279 2766 3795
Size: 1192 1087 487 1029
Fragments arranged by size:
1192 1087 1029 487
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2496 3795
Size: 2496 1299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 180 2239 2647 3589 3709 3795
Size: 85 95 2059 408 942 120 86
Fragments arranged by size:
2059 942 408 120 95 86 85
Bpu10I CC'TnA_GC
Cuts at: 0 316 776 1951 3325 3607 3795
Size: 316 460 1175 1374 282 188
Fragments arranged by size:
1374 1175 460 316 282 188
BsaI GGTCTCn'nnnn_
Cuts at: 0 1693 2543 3795
Size: 1693 850 1252
Fragments arranged by size:
1693 1252 850
BsaAI yAC'GTr
Cuts at: 0 875 986 3795
Size: 875 111 2809
Fragments arranged by size:
2809 875 111
BsaBI GATnn'nnATC
Cuts at: 0 1354 3795
Size: 1354 2441
BsaHI Gr'CG_yC
Cuts at: 0 1151 2261 3795
Size: 1151 1110 1534
Fragments arranged by size:
1534 1151 1110
BsaWI w'CCGG_w
Cuts at: 0 2012 2652 3795
Size: 2012 640 1143
Fragments arranged by size:
2012 1143 640
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 952 982 1579 1609 2380 2410 3795
Size: 952 30 597 30 771 30 1385
Fragments arranged by size:
1385 952 771 597 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 960 1231 1555 2512 2695 3582 3624 3795
Size: 960 271 324 957 183 887 42 171
Fragments arranged by size:
960 957 887 324 271 183 171 42
BseYI C'CCAG_C
Cuts at: 0 69 1079 1330 1371 2109 3795
Size: 69 1010 251 41 738 1686
Fragments arranged by size:
1686 1010 738 251 69 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1177 3795
Size: 1177 2618
BsiEI CG_ry'CG
Cuts at: 0 2097 3795
Size: 2097 1698
BsiHKAI G_wGCw'C
Cuts at: 0 576 590 1223 2147 2216 2687 2789 3210
Size: 576 14 633 924 69 471 102 421
Cuts at: 3210 3795
Size: 585
Fragments arranged by size:
924 633 585 576 471 421 102 69
14
BsmI GAATG_Cn'
Cuts at: 0 408 2166 3795
Size: 408 1758 1629
Fragments arranged by size:
1758 1629 408
BsmBI CGTCTCn'nnnn_
Cuts at: 0 136 1651 1849 2872 3795
Size: 136 1515 198 1023 923
Fragments arranged by size:
1515 1023 923 198 136
Bsp1286I G_dGCh'C
Cuts at: 0 63 576 590 777 1091 1223 1378 1553
Size: 63 513 14 187 314 132 155 175
Cuts at: 1553 1907 2147 2216 2382 2408 2633 2687 2789
Size: 354 240 69 166 26 225 54 102
Cuts at: 2789 3210 3342 3795
Size: 421 132 453
Fragments arranged by size:
513 453 421 354 314 240 225 187
175 166 155 132 132 102 69 63
54 26 14
BspEI T'CCGG_A
Cuts at: 0 2012 3795
Size: 2012 1783
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 141 734 1289 1871 3795
Size: 141 593 555 582 1924
Fragments arranged by size:
1924 593 582 555 141
BsrBI CCG'CTC
Cuts at: 0 117 801 1766 2867 3795
Size: 117 684 965 1101 928
Fragments arranged by size:
1101 965 928 684 117
BsrDI GCAATG_nn'
Cuts at: 0 1490 3678 3795
Size: 1490 2188 117
Fragments arranged by size:
2188 1490 117
BsrFI r'CCGG_y
Cuts at: 0 1101 2096 3795
Size: 1101 995 1699
Fragments arranged by size:
1699 1101 995
BsrGI T'GTAC_A
Cuts at: 0 1442 3795
Size: 1442 2353
BssSI C'ACGA_G
Cuts at: 0 887 1921 3795
Size: 887 1034 1874
Fragments arranged by size:
1874 1034 887
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2106 3795
Size: 2106 1689
BstEII G'GTnAC_C
Cuts at: 0 997 3795
Size: 997 2798
BstYI r'GATC_y
Cuts at: 0 780 965 1760 2280 2441 3173 3263 3795
Size: 780 185 795 520 161 732 90 532
Fragments arranged by size:
795 780 732 532 520 185 161 90
Bsu36I CC'TnA_GG
Cuts at: 0 2532 2591 3795
Size: 2532 59 1204
Fragments arranged by size:
2532 1204 59
BtgI C'CryG_G
Cuts at: 0 8 1113 1298 1772 1933 1956 2373 3795
Size: 8 1105 185 474 161 23 417 1422
Fragments arranged by size:
1422 1105 474 417 185 161 23 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 412 1400 2369 2760 3795
Size: 412 988 969 391 1035
Fragments arranged by size:
1035 988 969 412 391
BtsI GCAGTG_nn'
Cuts at: 0 540 1221 1752 3795
Size: 540 681 531 2043
Fragments arranged by size:
2043 681 540 531
ClaI AT'CG_AT
Cuts at: 0 646 3795
Size: 646 3149
DraI TTT'AAA
Cuts at: 0 525 3795
Size: 525 3270
DraIII CAC_nnn'GTG
Cuts at: 0 2106 3795
Size: 2106 1689
DrdI GACnn_nn'nnGTC
Cuts at: 0 2800 3795
Size: 2800 995
EaeI y'GGCC_r
Cuts at: 0 1250 2094 3559 3795
Size: 1250 844 1465 236
Fragments arranged by size:
1465 1250 844 236
EagI C'GGCC_G
Cuts at: 0 2094 3795
Size: 2094 1701
EarI CTCTTCn'nnn_
Cuts at: 0 37 616 1609 2985 3572 3760 3795
Size: 37 579 993 1376 587 188 35
Fragments arranged by size:
1376 993 587 579 188 37 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 208 2109 2989 3795
Size: 208 1901 880 806
Fragments arranged by size:
1901 880 806 208
EcoICRI GAG'CTC
Cuts at: 0 588 2145 2214 2685 2787 3795
Size: 588 1557 69 471 102 1008
Fragments arranged by size:
1557 1008 588 471 102 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 180 1497 1803 2034 2239 2647 2944
Size: 85 95 1317 306 231 205 408 297
Cuts at: 2944 3520 3589 3709 3795
Size: 576 69 120 86
Fragments arranged by size:
1317 576 408 306 297 231 205 120
95 86 85 69
EcoO109I rG'GnC_Cy
Cuts at: 0 1375 1784 2000 2114 2940 3795
Size: 1375 409 216 114 826 855
Fragments arranged by size:
1375 855 826 409 216 114
EcoRI G'AATT_C
Cuts at: 0 658 3795
Size: 658 3137
EcoRV GAT'ATC
Cuts at: 0 198 3795
Size: 198 3597
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 522 1527 2853 3601 3795
Size: 522 1005 1326 748 194
Fragments arranged by size:
1326 1005 748 522 194
FspI TGC'GCA
Cuts at: 0 1048 3670 3795
Size: 1048 2622 125
Fragments arranged by size:
2622 1048 125
HaeII r_GCGC'y
Cuts at: 0 2226 3795
Size: 2226 1569
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2233 2265 3795
Size: 2233 32 1530
Fragments arranged by size:
2233 1530 32
HincII GTy'rAC
Cuts at: 0 360 1289 1398 2916 2976 3795
Size: 360 929 109 1518 60 819
Fragments arranged by size:
1518 929 819 360 109 60
HindIII A'AGCT_T
Cuts at: 0 1028 3795
Size: 1028 2767
HpaI GTT'AAC
Cuts at: 0 360 3795
Size: 360 3435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2486 3367 3795
Size: 2486 881 428
Fragments arranged by size:
2486 881 428
MscI TGG'CCA
Cuts at: 0 1252 3795
Size: 1252 2543
MslI CAynn'nnrTG
Cuts at: 0 581 1978 3064 3795
Size: 581 1397 1086 731
Fragments arranged by size:
1397 1086 731 581
MspA1I CmG'CkG
Cuts at: 0 337 1005 1040 1320 1338 1958 3606 3729
Size: 337 668 35 280 18 620 1648 123
Cuts at: 3729 3795
Size: 66
Fragments arranged by size:
1648 668 620 337 280 123 66 35
18
NaeI GCC'GGC
Cuts at: 0 2098 3795
Size: 2098 1697
NcoI C'CATG_G
Cuts at: 0 1113 1772 3795
Size: 1113 659 2023
Fragments arranged by size:
2023 1113 659
NgoMIV G'CCGG_C
Cuts at: 0 2096 3795
Size: 2096 1699
NspI r_CATG'y
Cuts at: 0 933 1449 1977 3063 3795
Size: 933 516 528 1086 732
Fragments arranged by size:
1086 933 732 528 516
PasI CC'CwG_GG
Cuts at: 0 223 3795
Size: 223 3572
PciI A'CATG_T
Cuts at: 0 1445 3795
Size: 1445 2350
PflMI CCAn_nnn'nTGG
Cuts at: 0 1934 3795
Size: 1934 1861
PfoI T'CCnGG_A
Cuts at: 0 2216 2624 3566 3795
Size: 2216 408 942 229
Fragments arranged by size:
2216 942 408 229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1579 1611 3795
Size: 1579 32 2184
Fragments arranged by size:
2184 1579 32
PpuMI rG'GwC_Cy
Cuts at: 0 1784 2114 3795
Size: 1784 330 1681
Fragments arranged by size:
1784 1681 330
PshAI GACnn'nnGTC
Cuts at: 0 2950 3795
Size: 2950 845
PspOMI G'GGCC_C
Cuts at: 0 1087 1374 3795
Size: 1087 287 2421
Fragments arranged by size:
2421 1087 287
PspXI vC'TCGA_Gb
Cuts at: 0 1188 3795
Size: 1188 2607
PstI C_TGCA'G
Cuts at: 0 1228 1643 2490 3116 3795
Size: 1228 415 847 626 679
Fragments arranged by size:
1228 847 679 626 415
PvuII CAG'CTG
Cuts at: 0 337 1005 1320 1338 3606 3729 3795
Size: 337 668 315 18 2268 123 66
Fragments arranged by size:
2268 668 337 315 123 66 18
SacI G_AGCT'C
Cuts at: 0 590 2147 2216 2687 2789 3795
Size: 590 1557 69 471 102 1006
Fragments arranged by size:
1557 1006 590 471 102 69
SacII CC_GC'GG
Cuts at: 0 1959 3795
Size: 1959 1836
SalI G'TCGA_C
Cuts at: 0 2914 3795
Size: 2914 881
SanDI GG'GwC_CC
Cuts at: 0 1784 3795
Size: 1784 2011
SapI GCTCTTCn'nnn_
Cuts at: 0 37 3795
Size: 37 3758
ScaI AGT'ACT
Cuts at: 0 2452 2755 3795
Size: 2452 303 1040
Fragments arranged by size:
2452 1040 303
SexAI A'CCwGG_T
Cuts at: 0 2795 3795
Size: 2795 1000
SfcI C'TryA_G
Cuts at: 0 1224 1568 1639 2486 3112 3795
Size: 1224 344 71 847 626 683
Fragments arranged by size:
1224 847 683 626 344 71
SmaI CCC'GGG
Cuts at: 0 1993 2409 3795
Size: 1993 416 1386
Fragments arranged by size:
1993 1386 416
SmlI C'TyrA_G
Cuts at: 0 1188 3795
Size: 1188 2607
SspI AAT'ATT
Cuts at: 0 2555 3795
Size: 2555 1240
StuI AGG'CCT
Cuts at: 0 1258 3795
Size: 1258 2537
StyI C'CwwG_G
Cuts at: 0 1113 1253 1772 2399 2735 2828 3795
Size: 1113 140 519 627 336 93 967
Fragments arranged by size:
1113 967 627 519 336 140 93
TatI w'GTAC_w
Cuts at: 0 1442 2450 2753 2927 3056 3795
Size: 1442 1008 303 174 129 739
Fragments arranged by size:
1442 1008 739 303 174 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2380 2412 3023 3055 3795
Size: 2380 32 611 32 740
Fragments arranged by size:
2380 740 611 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2673 3795
Size: 2673 1122
XhoI C'TCGA_G
Cuts at: 0 1188 3795
Size: 1188 2607
XmaI C'CCGG_G
Cuts at: 0 1991 2407 3795
Size: 1991 416 1388
Fragments arranged by size:
1991 1388 416
XmnI GAAnn'nnTTC
Cuts at: 0 1594 3795
Size: 1594 2201
ZraI GAC'GTC
Cuts at: 0 1152 2262 3795
Size: 1152 1110 1533
Fragments arranged by size:
1533 1152 1110
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AloI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuII SacI SacII SalI
SanDI SapI ScaI SexAI SfcI SmaI SmlI SspI
StuI StyI TatI TstI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AleI AlfI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BciVI BfrBI
BmgBI BmtI BpuEI BsiWI BspHI BssHII BstBI BstXI
BstZ17I CspCI EcoNI FseI FspAI KasI KpnI MfeI
MluI NarI NdeI NheI NotI NruI NsiI PacI
PmeI PmlI PsiI PsrI PvuI RsrII SbfI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI
TaqII Tth111I XbaI