(Circular) (Six-base) MAPSORT of: pFN21AB3230.seq Check: 7573 from: 1 to: 6592 pFN21AB3230 6592 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2186 2186 Size: 6592 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3783 4402 278 Size: 53 83 186 936 2247 619 2468 Fragments arranged by size: 2468 2247 936 619 186 83 53 AccI GT'mk_AC Cuts at: 1937 3935 1937 Size: 1998 4594 Acc65I G'GTAC_C Cuts at: 3913 3913 Size: 6592 AclI AA'CG_TT Cuts at: 1563 4719 5092 1563 Size: 3156 373 3063 Fragments arranged by size: 3156 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2870 3401 4655 6039 6307 1632 Size: 1238 531 1254 1384 268 1917 Fragments arranged by size: 1917 1384 1254 1238 531 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6395 AflIII A'CryG_T Cuts at: 1203 2638 5491 6468 1203 Size: 1435 2853 977 1327 Fragments arranged by size: 2853 1435 1327 977 AgeI A'CCGG_T Cuts at: 5423 5423 Size: 6592 AhdI GACnn_n'nnGTC Cuts at: 5321 5321 Size: 6592 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6592 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6592 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3593 3625 3593 Size: 32 6560 AlwNI CAG_nnn'CTG Cuts at: 2091 4335 5907 2091 Size: 2244 1572 2776 Fragments arranged by size: 2776 2244 1572 ApaI G_GGCC'C Cuts at: 2286 2286 Size: 6592 ApaLI G'TGCA_C Cuts at: 3239 4649 5805 3239 Size: 1410 1156 4026 Fragments arranged by size: 4026 1410 1156 ApoI r'AATT_y Cuts at: 1493 3901 4127 4279 6388 1493 Size: 2408 226 152 2109 1697 Fragments arranged by size: 2408 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5146 160 Size: 872 4114 1606 Fragments arranged by size: 4114 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6592 AvaI C'yCGr_G Cuts at: 1870 1943 2991 3917 1870 Size: 73 1048 926 4545 Fragments arranged by size: 4545 1048 926 73 BamHI G'GATC_C Cuts at: 2377 3355 3922 4289 2377 Size: 978 567 367 4680 Fragments arranged by size: 4680 978 567 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3257 3913 5368 618 Size: 325 217 627 1470 656 1455 1842 Fragments arranged by size: 1842 1470 1455 656 627 325 217 BanII G_rGCy'C Cuts at: 1391 2052 2223 2286 3063 3138 3852 3911 1391 Size: 661 171 63 777 75 714 59 4072 Fragments arranged by size: 4072 777 714 661 171 75 63 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2405 3335 928 Size: 971 506 930 4185 Fragments arranged by size: 4185 971 930 506 BbvCI CC'TCA_GC Cuts at: 2198 2198 Size: 6592 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4783 4817 1903 Size: 34 2846 34 3678 Fragments arranged by size: 3678 2846 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2431 4484 5694 2431 Size: 2053 1210 3329 Fragments arranged by size: 3329 2053 1210 BclI T'GATC_A Cuts at: 1548 2860 1548 Size: 1312 5280 BfrBI ATG'CAT Cuts at: 2654 2654 Size: 6592 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5203 136 Size: 107 122 71 631 605 3531 1525 Fragments arranged by size: 3531 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6587 6587 Size: 6592 BlpI GC'TnA_GC Cuts at: 2778 4009 2778 Size: 1231 5361 Bme1580I G_kGCm'C Cuts at: 1790 2174 2286 3243 4653 5809 1790 Size: 384 112 957 1410 1156 2573 Fragments arranged by size: 2573 1410 1156 957 384 112 BmgBI CAC'GTC Cuts at: 1318 2660 1318 Size: 1342 5250 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3649 3855 4323 Size: 416 53 83 399 24 2232 206 468 Cuts at: 4323 5281 442 Size: 958 1753 Fragments arranged by size: 2232 1753 958 468 416 399 206 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6592 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2923 3077 2923 Size: 154 6438 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 2066 2621 3122 5252 1239 Size: 797 30 555 501 2130 2579 Fragments arranged by size: 2579 2130 797 555 501 30 Bpu10I CC'TnA_GC Cuts at: 2198 2198 Size: 6592 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2207 4717 5582 5880 2207 Size: 2510 865 298 2919 Fragments arranged by size: 2919 2510 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2294 2597 3130 5255 882 Size: 617 795 303 533 2125 2219 Fragments arranged by size: 2219 2125 795 617 533 303 BsaAI yAC'GTr Cuts at: 493 1204 4383 493 Size: 711 3179 2702 Fragments arranged by size: 3179 2702 711 BsaBI GATnn'nnATC Cuts at: 6586 6586 Size: 6592 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2325 3780 4399 4781 Size: 53 83 186 936 792 1455 619 382 Cuts at: 4781 5416 275 Size: 635 1451 Fragments arranged by size: 1455 1451 936 792 635 619 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2500 3155 5025 5423 5697 5844 6255 2500 Size: 655 1870 398 274 147 411 2837 Fragments arranged by size: 2837 1870 655 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6367 Fragments arranged by size: 6367 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2696 1186 Size: 1510 5082 BseYI C'CCAG_C Cuts at: 2294 5795 6291 2294 Size: 3501 496 2595 Fragments arranged by size: 3501 2595 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3049 3859 1163 Size: 573 1313 810 3896 Fragments arranged by size: 3896 1313 810 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4042 4803 5831 1540 Size: 275 186 2041 761 1028 2301 Fragments arranged by size: 2301 2041 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2052 3063 3138 3243 4653 4738 5809 2052 Size: 1011 75 105 1410 85 1071 2835 Fragments arranged by size: 2835 1410 1071 1011 105 85 75 BsmI GAATG_Cn' Cuts at: 4103 4196 4103 Size: 93 6499 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2174 2223 2286 3063 3138 3243 Size: 399 262 122 49 63 777 75 105 Cuts at: 3243 3852 3911 4653 4738 5809 1391 Size: 609 59 742 85 1071 2174 Fragments arranged by size: 2174 1071 777 742 609 399 262 122 105 85 75 63 59 49 BspEI T'CCGG_A Cuts at: 2500 6255 2500 Size: 3755 2837 BspHI T'CATG_A Cuts at: 4481 6211 4481 Size: 1730 4862 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2039 2186 3947 844 Size: 1195 147 1761 3489 Fragments arranged by size: 3489 1761 1195 147 BsrBI CCG'CTC Cuts at: 1394 1823 2280 2314 2812 3255 4479 1394 Size: 429 457 34 498 443 1224 3507 Fragments arranged by size: 3507 1224 498 457 443 429 34 BsrDI GCAATG_nn' Cuts at: 65 1248 5087 5261 65 Size: 1183 3839 174 1396 Fragments arranged by size: 3839 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2181 3507 3889 5236 5423 1700 Size: 110 371 1326 382 1347 187 2869 Fragments arranged by size: 2869 1347 1326 382 371 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6592 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6592 BssSI C'ACGA_G Cuts at: 4652 5664 4652 Size: 1012 5580 BstAPI GCAn_nnn'nTGC Cuts at: 794 2802 3247 794 Size: 2008 445 4139 Fragments arranged by size: 4139 2008 445 BstBI TT'CG_AA Cuts at: 5396 5396 Size: 6592 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6592 BstYI r'GATC_y Cuts at: 1972 2377 3355 3922 4289 4674 4691 6143 6229 Size: 405 978 567 367 385 17 1452 86 Cuts at: 6229 6241 6587 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 978 567 405 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2910 513 Size: 552 939 906 4195 Fragments arranged by size: 4195 939 906 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5853 Fragments arranged by size: 5853 683 56 BtsI GCAGTG_nn' Cuts at: 3316 4108 4902 4922 3316 Size: 792 794 20 4986 Fragments arranged by size: 4986 794 792 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6592 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6557 DraI TTT'AAA Cuts at: 3349 3896 3349 Size: 547 6045 DraIII CAC_nnn'GTG Cuts at: 2129 3819 2129 Size: 1690 4902 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5599 809 Size: 507 4283 1802 Fragments arranged by size: 4283 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3505 4039 4928 Size: 54 1425 50 275 18 1675 534 889 Cuts at: 4928 6557 8 Size: 1629 43 Fragments arranged by size: 1675 1629 1425 889 534 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4039 1537 Size: 275 2227 4090 Fragments arranged by size: 4090 2227 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2070 3051 3428 4522 1353 Size: 717 981 377 1094 3423 Fragments arranged by size: 3423 1094 981 717 377 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2332 5179 5553 5699 387 Size: 1064 138 743 2847 374 146 1280 Fragments arranged by size: 2847 1280 1064 743 374 146 138 EcoICRI GAG'CTC Cuts at: 2050 3061 3136 2050 Size: 1011 75 5506 Fragments arranged by size: 5506 1011 75 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2066 2621 2870 3122 3401 4655 Size: 393 404 30 555 249 252 279 1254 Cuts at: 4655 5252 6039 6307 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1254 787 597 555 404 393 279 268 252 249 30 EcoO109I rG'GnC_Cy Cuts at: 2059 2353 2059 Size: 294 6298 EcoRI G'AATT_C Cuts at: 3901 4279 3901 Size: 378 6214 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2485 2572 3286 2485 Size: 87 714 5791 Fragments arranged by size: 5791 714 87 FspI TGC'GCA Cuts at: 2080 3792 5098 2080 Size: 1712 1306 3574 Fragments arranged by size: 3574 1712 1306 HaeII r_GCGC'y Cuts at: 1678 3620 5739 1678 Size: 1942 2119 2531 Fragments arranged by size: 2531 2119 1942 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3347 3379 3407 3410 3439 Size: 32 135 32 1775 32 28 3 29 Cuts at: 3439 3442 3713 3745 5233 5265 5307 5339 1373 Size: 3 271 32 1488 32 42 32 2626 Fragments arranged by size: 2626 1775 1488 271 135 42 32 32 32 32 32 32 29 28 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 2636 3936 4182 669 Size: 988 281 698 1300 246 3079 Fragments arranged by size: 3079 1300 988 698 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6592 HpaI GTT'AAC Cuts at: 4182 4182 Size: 6592 KpnI G_GTAC'C Cuts at: 3917 3917 Size: 6592 MfeI C'AATT_G Cuts at: 4191 4191 Size: 6592 MluI A'CGCG_T Cuts at: 6468 6468 Size: 6592 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5706 5890 1305 Size: 4401 184 2007 Fragments arranged by size: 4401 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4770 Fragments arranged by size: 4770 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2541 3089 3272 4550 4909 5068 518 Size: 648 1375 548 183 1278 359 159 2042 Fragments arranged by size: 2042 1375 1278 648 548 359 183 159 MspA1I CmG'CkG Cuts at: 1606 3627 3886 4008 4685 5833 6078 1606 Size: 2021 259 122 677 1148 245 2120 Fragments arranged by size: 2120 2021 1148 677 259 245 122 NaeI GCC'GGC Cuts at: 2183 3509 2183 Size: 1326 5266 NcoI C'CATG_G Cuts at: 513 1065 2004 2910 513 Size: 552 939 906 4195 Fragments arranged by size: 4195 939 906 552 NdeI CA'TA_TG Cuts at: 387 3076 387 Size: 2689 3903 NgoMIV G'CCGG_C Cuts at: 2181 3507 2181 Size: 1326 5266 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6592 NotI GC'GGCC_GC Cuts at: 4039 4039 Size: 6592 NsiI A_TGCA'T Cuts at: 2656 2656 Size: 6592 NspI r_CATG'y Cuts at: 1132 1229 2189 2642 3950 5495 1132 Size: 97 960 453 1308 1545 2229 Fragments arranged by size: 2229 1545 1308 960 453 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6592 PciI A'CATG_T Cuts at: 2638 5491 2638 Size: 2853 3739 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6592 PmeI GTTT'AAAC Cuts at: 3896 3896 Size: 6592 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4661 4693 6174 6206 4661 Size: 32 1481 32 5047 Fragments arranged by size: 5047 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2059 2353 2059 Size: 294 6298 PshAI GACnn'nnGTC Cuts at: 1613 2405 1613 Size: 792 5800 PsiI TTA'TAA Cuts at: 4162 4162 Size: 6592 PspOMI G'GGCC_C Cuts at: 2282 2282 Size: 6592 PstI C_TGCA'G Cuts at: 830 3252 3944 830 Size: 2422 692 3478 Fragments arranged by size: 3478 2422 692 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6592 PvuII CAG'CTG Cuts at: 3886 3886 Size: 6592 SacI G_AGCT'C Cuts at: 2052 3063 3138 2052 Size: 1011 75 5506 Fragments arranged by size: 5506 1011 75 SalI G'TCGA_C Cuts at: 1936 3934 1936 Size: 1998 4594 SapI GCTCTTCn'nnn_ Cuts at: 3051 3051 Size: 6592 SbfI CC_TGCA'GG Cuts at: 3944 3944 Size: 6592 ScaI AGT'ACT Cuts at: 4840 6552 4840 Size: 1712 4880 SfcI C'TryA_G Cuts at: 826 1045 2240 2876 3248 3940 5075 5756 5947 Size: 219 1195 636 372 692 1135 681 191 Cuts at: 5947 826 Size: 1471 Fragments arranged by size: 1471 1195 1135 692 681 636 372 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6592 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6592 SmaI CCC'GGG Cuts at: 1872 3919 1872 Size: 2047 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2222 4696 5597 5859 820 Size: 197 926 279 2474 901 262 1553 Fragments arranged by size: 2474 1553 926 901 279 262 197 SnaBI TAC'GTA Cuts at: 493 4383 493 Size: 3890 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6592 SphI G_CATG'C Cuts at: 1132 3950 1132 Size: 2818 3774 SspI AAT'ATT Cuts at: 5 52 4516 5 Size: 47 4464 2081 Fragments arranged by size: 4464 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6592 StyI C'CwwG_G Cuts at: 513 1065 2004 2910 4031 513 Size: 552 939 906 1121 3074 Fragments arranged by size: 3074 1121 939 906 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3631 4619 4636 4789 4974 5419 325 Size: 1255 2051 988 17 153 185 445 1498 Fragments arranged by size: 2051 1498 1255 988 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3141 3264 4838 Size: 274 80 33 51 1443 1164 123 1574 Cuts at: 4838 6550 96 Size: 1712 138 Fragments arranged by size: 1712 1574 1443 1164 274 138 123 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6552 6584 6552 Size: 32 6560 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6592 XbaI T'CTAG_A Cuts at: 2212 3928 2212 Size: 1716 4876 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2143 3470 2011 Size: 132 1327 5133 Fragments arranged by size: 5133 1327 132 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6592 XmaI C'CCGG_G Cuts at: 1870 3917 1870 Size: 2047 4545 XmnI GAAnn'nnTTC Cuts at: 4721 4721 Size: 6592 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3781 4400 276 Size: 53 83 186 936 2247 619 2468 Fragments arranged by size: 2468 2247 936 619 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI