Restriction map for
pFN21AB3230
(Linear) (Six-base) MAPSORT of: KIBB3230.seq Check: 7386 from: 1 to: 1887
KIBB3230.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 181 1887
Size: 181 1706
AatII G_ACGT'C
Cuts at: 0 1778 1887
Size: 1778 109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 1396 1887
Size: 865 531 491
Fragments arranged by size:
865 531 491
AflIII A'CryG_T
Cuts at: 0 633 1887
Size: 633 1254
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1588 1620 1887
Size: 1588 32 267
Fragments arranged by size:
1588 267 32
AlwNI CAG_nnn'CTG
Cuts at: 0 86 1887
Size: 86 1801
ApaI G_GGCC'C
Cuts at: 0 281 1887
Size: 281 1606
ApaLI G'TGCA_C
Cuts at: 0 1234 1887
Size: 1234 653
AvaI C'yCGr_G
Cuts at: 0 986 1887
Size: 986 901
BamHI G'GATC_C
Cuts at: 0 372 1350 1887
Size: 372 978 537
Fragments arranged by size:
978 537 372
BanI G'GyrC_C
Cuts at: 0 1252 1887
Size: 1252 635
BanII G_rGCy'C
Cuts at: 0 47 218 281 1058 1133 1847 1887
Size: 47 171 63 777 75 714 40
Fragments arranged by size:
777 714 171 75 63 47 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 400 1330 1887
Size: 400 930 557
Fragments arranged by size:
930 557 400
BbvCI CC'TCA_GC
Cuts at: 0 193 1887
Size: 193 1694
BciVI GTATCCnnnnn_n'
Cuts at: 0 426 1887
Size: 426 1461
BclI T'GATC_A
Cuts at: 0 855 1887
Size: 855 1032
BfrBI ATG'CAT
Cuts at: 0 649 1887
Size: 649 1238
BlpI GC'TnA_GC
Cuts at: 0 773 1887
Size: 773 1114
Bme1580I G_kGCm'C
Cuts at: 0 169 281 1238 1887
Size: 169 112 957 649
Fragments arranged by size:
957 649 169 112
BmgBI CAC'GTC
Cuts at: 0 655 1887
Size: 655 1232
BmrI ACTGGGnnnn_n'
Cuts at: 0 1644 1850 1887
Size: 1644 206 37
Fragments arranged by size:
1644 206 37
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 918 1072 1887
Size: 918 154 815
Fragments arranged by size:
918 815 154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 61 616 1117 1887
Size: 31 30 555 501 770
Fragments arranged by size:
770 555 501 31 30
Bpu10I CC'TnA_GC
Cuts at: 0 193 1887
Size: 193 1694
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1887
Size: 202 1685
BsaI GGTCTCn'nnnn_
Cuts at: 0 289 592 1125 1887
Size: 289 303 533 762
Fragments arranged by size:
762 533 303 289
BsaHI Gr'CG_yC
Cuts at: 0 320 1775 1887
Size: 320 1455 112
Fragments arranged by size:
1455 320 112
BsaWI w'CCGG_w
Cuts at: 0 495 1150 1887
Size: 495 655 737
Fragments arranged by size:
737 655 495
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 691 1887
Size: 691 1196
BseYI C'CCAG_C
Cuts at: 0 289 1887
Size: 289 1598
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1044 1854 1887
Size: 1044 810 33
Fragments arranged by size:
1044 810 33
BsiHKAI G_wGCw'C
Cuts at: 0 47 1058 1133 1238 1887
Size: 47 1011 75 105 649
Fragments arranged by size:
1011 649 105 75 47
Bsp1286I G_dGCh'C
Cuts at: 0 47 169 218 281 1058 1133 1238 1847
Size: 47 122 49 63 777 75 105 609
Cuts at: 1847 1887
Size: 40
Fragments arranged by size:
777 609 122 105 75 63 49 47
40
BspEI T'CCGG_A
Cuts at: 0 495 1887
Size: 495 1392
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 34 181 1887
Size: 34 147 1706
Fragments arranged by size:
1706 147 34
BsrBI CCG'CTC
Cuts at: 0 275 309 807 1250 1887
Size: 275 34 498 443 637
Fragments arranged by size:
637 498 443 275 34
BsrFI r'CCGG_y
Cuts at: 0 176 1502 1887
Size: 176 1326 385
Fragments arranged by size:
1326 385 176
BstAPI GCAn_nnn'nTGC
Cuts at: 0 797 1242 1887
Size: 797 445 645
Fragments arranged by size:
797 645 445
BstYI r'GATC_y
Cuts at: 0 372 1350 1887
Size: 372 978 537
Fragments arranged by size:
978 537 372
BtgI C'CryG_G
Cuts at: 0 905 1887
Size: 905 982
BtsI GCAGTG_nn'
Cuts at: 0 1311 1887
Size: 1311 576
DraI TTT'AAA
Cuts at: 0 1344 1887
Size: 1344 543
DraIII CAC_nnn'GTG
Cuts at: 0 124 1814 1887
Size: 124 1690 73
Fragments arranged by size:
1690 124 73
EaeI y'GGCC_r
Cuts at: 0 1500 1887
Size: 1500 387
EarI CTCTTCn'nnn_
Cuts at: 0 65 1046 1423 1887
Size: 65 981 377 464
Fragments arranged by size:
981 464 377 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 327 1887
Size: 327 1560
EcoICRI GAG'CTC
Cuts at: 0 45 1056 1131 1887
Size: 45 1011 75 756
Fragments arranged by size:
1011 756 75 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 61 616 865 1117 1396 1887
Size: 31 30 555 249 252 279 491
Fragments arranged by size:
555 491 279 252 249 31 30
EcoO109I rG'GnC_Cy
Cuts at: 0 54 348 1887
Size: 54 294 1539
Fragments arranged by size:
1539 294 54
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 480 567 1281 1887
Size: 480 87 714 606
Fragments arranged by size:
714 606 480 87
FspI TGC'GCA
Cuts at: 0 75 1787 1887
Size: 75 1712 100
Fragments arranged by size:
1712 100 75
HaeII r_GCGC'y
Cuts at: 0 1615 1887
Size: 1615 272
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1342 1374 1402 1405 1434 1437 1708 1740
Size: 1342 32 28 3 29 3 271 32
Cuts at: 1740 1887
Size: 147
Fragments arranged by size:
1342 271 147 32 32 29 28 3
3
HincII GTy'rAC
Cuts at: 0 631 1887
Size: 631 1256
MslI CAynn'nnrTG
Cuts at: 0 536 1084 1267 1887
Size: 536 548 183 620
Fragments arranged by size:
620 548 536 183
MspA1I CmG'CkG
Cuts at: 0 1622 1881 1887
Size: 1622 259 6
Fragments arranged by size:
1622 259 6
NaeI GCC'GGC
Cuts at: 0 178 1504 1887
Size: 178 1326 383
Fragments arranged by size:
1326 383 178
NcoI C'CATG_G
Cuts at: 0 905 1887
Size: 905 982
NdeI CA'TA_TG
Cuts at: 0 1071 1887
Size: 1071 816
NgoMIV G'CCGG_C
Cuts at: 0 176 1502 1887
Size: 176 1326 385
Fragments arranged by size:
1326 385 176
NsiI A_TGCA'T
Cuts at: 0 651 1887
Size: 651 1236
NspI r_CATG'y
Cuts at: 0 184 637 1887
Size: 184 453 1250
Fragments arranged by size:
1250 453 184
PciI A'CATG_T
Cuts at: 0 633 1887
Size: 633 1254
PpuMI rG'GwC_Cy
Cuts at: 0 54 348 1887
Size: 54 294 1539
Fragments arranged by size:
1539 294 54
PshAI GACnn'nnGTC
Cuts at: 0 400 1887
Size: 400 1487
PspOMI G'GGCC_C
Cuts at: 0 277 1887
Size: 277 1610
PstI C_TGCA'G
Cuts at: 0 1247 1887
Size: 1247 640
PvuII CAG'CTG
Cuts at: 0 1881 1887
Size: 1881 6
SacI G_AGCT'C
Cuts at: 0 47 1058 1133 1887
Size: 47 1011 75 754
Fragments arranged by size:
1011 754 75 47
SapI GCTCTTCn'nnn_
Cuts at: 0 1046 1887
Size: 1046 841
SfcI C'TryA_G
Cuts at: 0 235 871 1243 1887
Size: 235 636 372 644
Fragments arranged by size:
644 636 372 235
SmlI C'TyrA_G
Cuts at: 0 217 1887
Size: 217 1670
StyI C'CwwG_G
Cuts at: 0 905 1887
Size: 905 982
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1626 1887
Size: 1626 261
TatI w'GTAC_w
Cuts at: 0 1136 1259 1887
Size: 1136 123 628
Fragments arranged by size:
1136 628 123
XbaI T'CTAG_A
Cuts at: 0 207 1887
Size: 207 1680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 138 1465 1887
Size: 138 1327 422
Fragments arranged by size:
1327 422 138
ZraI GAC'GTC
Cuts at: 0 1776 1887
Size: 1776 111
Enzymes that do cut:
AarI AatII AcuI AflIII AloI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbsI BbvCI BciVI BclI
BfrBI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspMI BsrBI BsrFI BstAPI BstYI BtgI
BtsI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoO109I FalI FspI HaeII Hin4I HincII MslI MspA1I
NaeI NcoI NdeI NgoMIV NsiI NspI PciI PpuMI
PshAI PspOMI PstI PvuII SacI SapI SfcI SmlI
StyI TaqII TatI XbaI XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BcgI BglI BglII BmtI BsaAI BsaBI BsaXI BsiEI
BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DrdI EagI EcoNI EcoRI EcoRV FseI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NarI
NheI NotI NruI PacI PasI PflMI PfoI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XhoI XmaI XmnI