(Circular) (Six-base) MAPSORT of: pFN21AB3191.seq Check: 7547 from: 1 to: 6472 pFN21AB3191 6472 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3729 4282 278 Size: 53 83 186 936 2193 553 2468 Fragments arranged by size: 2468 2193 936 553 186 83 53 AccI GT'mk_AC Cuts at: 1937 3815 1937 Size: 1878 4594 Acc65I G'GTAC_C Cuts at: 3793 3793 Size: 6472 AclI AA'CG_TT Cuts at: 1563 4599 4972 1563 Size: 3036 373 3063 Fragments arranged by size: 3063 3036 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2781 3143 3422 4535 5919 6187 1632 Size: 1149 362 279 1113 1384 268 1917 Fragments arranged by size: 1917 1384 1149 1113 362 279 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6275 AflIII A'CryG_T Cuts at: 1203 3022 5371 6348 1203 Size: 1819 2349 977 1327 Fragments arranged by size: 2349 1819 1327 977 AgeI A'CCGG_T Cuts at: 5303 5303 Size: 6472 AhdI GACnn_n'nnGTC Cuts at: 5201 5201 Size: 6472 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6472 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3539 1184 Size: 2355 4117 AlwNI CAG_nnn'CTG Cuts at: 2707 2872 3214 4215 5787 2707 Size: 165 342 1001 1572 3392 Fragments arranged by size: 3392 1572 1001 342 165 ApaI G_GGCC'C Cuts at: 3439 3651 3439 Size: 212 6260 ApaLI G'TGCA_C Cuts at: 2261 4529 5685 2261 Size: 2268 1156 3048 Fragments arranged by size: 3048 2268 1156 ApoI r'AATT_y Cuts at: 1493 3781 4007 4159 6268 1493 Size: 2288 226 152 2109 1697 Fragments arranged by size: 2288 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5026 160 Size: 872 3994 1606 Fragments arranged by size: 3994 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 AvaI C'yCGr_G Cuts at: 1870 1943 2472 3797 1870 Size: 73 529 1325 4545 Fragments arranged by size: 4545 1325 529 73 BamHI G'GATC_C Cuts at: 3802 4169 3802 Size: 367 6105 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3793 5248 618 Size: 325 217 627 2006 1455 1842 Fragments arranged by size: 2006 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2814 3439 3651 3791 1391 Size: 1423 625 212 140 4072 Fragments arranged by size: 4072 1423 625 212 140 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2769 928 Size: 971 870 4631 Fragments arranged by size: 4631 971 870 BbvCI CC'TCA_GC Cuts at: 3240 3240 Size: 6472 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4663 4697 1903 Size: 34 2726 34 3678 Fragments arranged by size: 3678 2726 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4364 5574 4364 Size: 1210 5262 BclI T'GATC_A Cuts at: 1548 2967 1548 Size: 1419 5053 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3746 5083 Size: 107 122 71 631 605 337 1737 1337 Cuts at: 5083 136 Size: 1525 Fragments arranged by size: 1737 1525 1337 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2979 6467 2979 Size: 3488 2984 BlpI GC'TnA_GC Cuts at: 3117 3889 3117 Size: 772 5700 Bme1580I G_kGCm'C Cuts at: 1790 2265 3137 3439 3651 4533 5689 1790 Size: 475 872 302 212 882 1156 2573 Fragments arranged by size: 2573 1156 882 872 475 302 212 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6472 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3202 4203 5161 Size: 416 53 83 399 24 1785 1001 958 Cuts at: 5161 442 Size: 1753 Fragments arranged by size: 1785 1753 1001 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6472 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2674 2674 Size: 6472 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2303 2396 2612 2744 2798 5132 1239 Size: 1064 93 216 132 54 2334 2579 Fragments arranged by size: 2579 2334 1064 216 132 93 54 Bpu10I CC'TnA_GC Cuts at: 3240 3240 Size: 6472 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4597 5462 5760 4597 Size: 865 298 5309 Fragments arranged by size: 5309 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3062 3471 5135 882 Size: 617 1563 409 1664 2219 Fragments arranged by size: 2219 1664 1563 617 409 BsaAI yAC'GTr Cuts at: 493 1204 2260 4263 493 Size: 711 1056 2003 2702 Fragments arranged by size: 2702 2003 1056 711 BsaBI GATnn'nnATC Cuts at: 3191 6466 3191 Size: 3275 3197 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3246 3726 4279 4661 Size: 53 83 186 936 1713 480 553 382 Cuts at: 4661 5296 275 Size: 635 1451 Fragments arranged by size: 1713 1451 936 635 553 480 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2514 3473 4905 5303 5577 5724 6135 2514 Size: 959 1432 398 274 147 411 2851 Fragments arranged by size: 2851 1432 959 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2990 3020 1178 Size: 30 165 30 1587 30 4630 Fragments arranged by size: 4630 1587 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2318 1186 Size: 1132 5340 BseYI C'CCAG_C Cuts at: 2221 2569 2700 3304 3590 5675 6171 2221 Size: 348 131 604 286 2085 496 2522 Fragments arranged by size: 2522 2085 604 496 348 286 131 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3476 1163 Size: 573 1740 4159 Fragments arranged by size: 4159 1740 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 3922 4683 5711 1540 Size: 275 186 15 1906 761 1028 2301 Fragments arranged by size: 2301 1906 1028 761 275 186 15 BsiHKAI G_wGCw'C Cuts at: 2052 2265 2361 2528 2814 3096 4533 4618 5689 Size: 213 96 167 286 282 1437 85 1071 Cuts at: 5689 2052 Size: 2835 Fragments arranged by size: 2835 1437 1071 286 282 213 167 96 85 BsmI GAATG_Cn' Cuts at: 3983 4076 3983 Size: 93 6379 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2265 2361 2528 2814 3096 3137 Size: 399 262 213 96 167 286 282 41 Cuts at: 3137 3439 3651 3791 4533 4618 5689 1391 Size: 302 212 140 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 302 286 282 262 213 212 167 140 96 85 41 BspEI T'CCGG_A Cuts at: 2514 3473 6135 2514 Size: 959 2662 2851 Fragments arranged by size: 2851 2662 959 BspHI T'CATG_A Cuts at: 4361 6091 4361 Size: 1730 4742 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3498 3827 844 Size: 2654 329 3489 Fragments arranged by size: 3489 2654 329 BsrBI CCG'CTC Cuts at: 1394 1823 4359 1394 Size: 429 2536 3507 Fragments arranged by size: 3507 2536 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2074 4967 5141 65 Size: 1183 826 2893 174 1396 Fragments arranged by size: 2893 1396 1183 826 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5116 5303 1700 Size: 110 3306 187 2869 Fragments arranged by size: 3306 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6472 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6472 BssSI C'ACGA_G Cuts at: 2354 4532 5544 2354 Size: 2178 1012 3282 Fragments arranged by size: 3282 2178 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2269 2389 3100 794 Size: 1475 120 711 4166 Fragments arranged by size: 4166 1475 711 120 BstBI TT'CG_AA Cuts at: 5276 5276 Size: 6472 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6472 BstXI CCAn_nnnn'nTGG Cuts at: 2122 2590 3463 2122 Size: 468 873 5131 Fragments arranged by size: 5131 873 468 BstYI r'GATC_y Cuts at: 1972 2719 2979 3802 4169 4554 4571 6023 6109 Size: 747 260 823 367 385 17 1452 86 Cuts at: 6109 6121 6467 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 823 747 385 367 346 260 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3138 3138 Size: 6472 BtgI C'CryG_G Cuts at: 513 1065 2004 2541 3573 513 Size: 552 939 537 1032 3412 Fragments arranged by size: 3412 1032 939 552 537 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2026 2101 2680 487 Size: 683 56 800 75 579 4279 Fragments arranged by size: 4279 800 683 579 75 56 BtsI GCAGTG_nn' Cuts at: 2263 2334 2829 3988 4782 4802 2263 Size: 71 495 1159 794 20 3933 Fragments arranged by size: 3933 1159 794 495 71 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6472 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6437 DraI TTT'AAA Cuts at: 3776 3776 Size: 6472 DraIII CAC_nnn'GTG Cuts at: 2389 2389 Size: 6472 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3760 5479 809 Size: 507 2444 1719 1802 Fragments arranged by size: 2444 1802 1719 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2292 3267 Size: 54 1425 50 275 18 183 279 975 Cuts at: 3267 3465 3624 3919 4808 6437 8 Size: 198 159 295 889 1629 43 Fragments arranged by size: 1629 1425 975 889 295 279 275 198 183 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2013 3919 1537 Size: 275 201 1906 4090 Fragments arranged by size: 4090 1906 275 201 EarI CTCTTCn'nnn_ Cuts at: 1353 2459 3113 3515 4402 1353 Size: 1106 654 402 887 3423 Fragments arranged by size: 3423 1106 887 654 402 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2510 5059 5433 5579 387 Size: 1064 138 921 2549 374 146 1280 Fragments arranged by size: 2549 1280 1064 921 374 146 138 EcoICRI GAG'CTC Cuts at: 2812 2812 Size: 6472 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2303 2396 2612 2744 2781 2798 3143 Size: 393 671 93 216 132 37 17 345 Cuts at: 3143 3422 4535 5132 5919 6187 1239 Size: 279 1113 597 787 268 1524 Fragments arranged by size: 1524 1113 787 671 597 393 345 279 268 216 132 93 37 17 EcoO109I rG'GnC_Cy Cuts at: 2142 2197 2728 2828 3595 3647 2142 Size: 55 531 100 767 52 4967 Fragments arranged by size: 4967 767 531 100 55 52 EcoRI G'AATT_C Cuts at: 3781 4159 3781 Size: 378 6094 FspI TGC'GCA Cuts at: 3584 4978 3584 Size: 1394 5078 HaeII r_GCGC'y Cuts at: 1678 5619 1678 Size: 3941 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2728 2760 2990 3022 5113 Size: 32 135 32 1156 32 230 32 2091 Cuts at: 5113 5145 5187 5219 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2091 1156 230 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3816 4062 669 Size: 988 281 1878 246 3079 Fragments arranged by size: 3079 1878 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6472 HpaI GTT'AAC Cuts at: 4062 4062 Size: 6472 KpnI G_GTAC'C Cuts at: 3797 3797 Size: 6472 MfeI C'AATT_G Cuts at: 4071 4071 Size: 6472 MluI A'CGCG_T Cuts at: 6348 6348 Size: 6472 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2658 5586 5770 1305 Size: 1353 2928 184 2007 Fragments arranged by size: 2928 2007 1353 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2294 3269 3467 3626 10 Size: 54 1425 343 462 975 198 159 2856 Fragments arranged by size: 2856 1425 975 462 343 198 159 54 MslI CAynn'nnrTG Cuts at: 518 1166 2045 2540 2624 3369 3521 4430 4789 Size: 648 879 495 84 745 152 909 359 Cuts at: 4789 4948 518 Size: 159 2042 Fragments arranged by size: 2042 909 879 745 648 495 359 159 152 84 MspA1I CmG'CkG Cuts at: 1606 2221 2281 2759 2948 3121 3202 3304 3888 Size: 615 60 478 189 173 81 102 584 Cuts at: 3888 4565 5713 5958 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 615 584 478 245 189 173 102 81 60 NcoI C'CATG_G Cuts at: 513 1065 2004 2541 513 Size: 552 939 537 4444 Fragments arranged by size: 4444 939 552 537 NdeI CA'TA_TG Cuts at: 387 387 Size: 6472 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6472 NotI GC'GGCC_GC Cuts at: 3919 3919 Size: 6472 NspI r_CATG'y Cuts at: 1132 1229 3026 3286 3568 3830 5375 1132 Size: 97 1797 260 282 262 1545 2229 Fragments arranged by size: 2229 1797 1545 282 262 260 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6472 PciI A'CATG_T Cuts at: 3022 5371 3022 Size: 2349 4123 PflMI CCAn_nnn'nTGG Cuts at: 1108 3721 1108 Size: 2613 3859 PfoI T'CCnGG_A Cuts at: 3654 3654 Size: 6472 PmeI GTTT'AAAC Cuts at: 3776 3776 Size: 6472 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2510 2542 3143 3175 4541 4573 6054 6086 2510 Size: 32 601 32 1366 32 1481 32 2896 Fragments arranged by size: 2896 1481 1366 601 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2142 2197 3595 2142 Size: 55 1398 5019 Fragments arranged by size: 5019 1398 55 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6472 PsiI TTA'TAA Cuts at: 4042 4042 Size: 6472 PspOMI G'GGCC_C Cuts at: 3435 3647 3435 Size: 212 6260 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2218 2250 2218 Size: 32 6440 PstI C_TGCA'G Cuts at: 830 3639 3824 830 Size: 2809 185 3478 Fragments arranged by size: 3478 2809 185 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6472 PvuII CAG'CTG Cuts at: 2221 2948 3121 3202 3304 2221 Size: 727 173 81 102 5389 Fragments arranged by size: 5389 727 173 102 81 SacI G_AGCT'C Cuts at: 2814 2814 Size: 6472 SalI G'TCGA_C Cuts at: 1936 3814 1936 Size: 1878 4594 SanDI GG'GwC_CC Cuts at: 3595 3595 Size: 6472 SapI GCTCTTCn'nnn_ Cuts at: 3515 3515 Size: 6472 SbfI CC_TGCA'GG Cuts at: 3824 3824 Size: 6472 ScaI AGT'ACT Cuts at: 2510 4720 6432 2510 Size: 2210 1712 2550 Fragments arranged by size: 2550 2210 1712 SexAI A'CCwGG_T Cuts at: 2850 2850 Size: 6472 SfcI C'TryA_G Cuts at: 826 1045 3635 3820 4955 5636 5827 826 Size: 219 2590 185 1135 681 191 1471 Fragments arranged by size: 2590 1471 1135 681 219 191 185 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6472 SmaI CCC'GGG Cuts at: 1872 3799 1872 Size: 1927 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4576 5477 5739 820 Size: 197 926 2633 901 262 1553 Fragments arranged by size: 2633 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4263 493 Size: 3770 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6472 SphI G_CATG'C Cuts at: 1132 3830 1132 Size: 2698 3774 SspI AAT'ATT Cuts at: 5 52 4396 5 Size: 47 4344 2081 Fragments arranged by size: 4344 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6472 StyI C'CwwG_G Cuts at: 513 1065 2004 2121 2128 2541 2583 3456 3911 Size: 552 939 117 7 413 42 873 455 Cuts at: 3911 513 Size: 3074 Fragments arranged by size: 3074 939 873 552 455 413 117 42 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4499 4516 4669 4854 5299 325 Size: 1255 2919 17 153 185 445 1498 Fragments arranged by size: 2919 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2508 3279 4718 Size: 274 80 33 51 1443 531 771 1439 Cuts at: 4718 6430 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1439 771 531 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2134 2166 3236 3268 6432 6464 2134 Size: 32 1070 32 3164 32 2142 Fragments arranged by size: 3164 2142 1070 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2603 2852 1316 Size: 1287 249 4936 Fragments arranged by size: 4936 1287 249 XbaI T'CTAG_A Cuts at: 3808 3808 Size: 6472 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2119 2848 3232 2119 Size: 729 384 5359 Fragments arranged by size: 5359 729 384 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6472 XmaI C'CCGG_G Cuts at: 1870 3797 1870 Size: 1927 4545 XmnI GAAnn'nnTTC Cuts at: 4601 4601 Size: 6472 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3727 4280 276 Size: 53 83 186 936 2193 553 2468 Fragments arranged by size: 2468 2193 936 553 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacII SfiI SfoI SrfI SwaI