(Circular) (Six-base) MAPSORT of: pFN21AB3129.seq Check: 7835 from: 1 to: 5422 pFN21AB3129 5422 bp With 182 enzymes: SgfI * May 7, 2009 14:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2565 2565 Size: 5422 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3232 278 Size: 53 83 186 936 1696 2468 Fragments arranged by size: 2468 1696 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2765 1937 Size: 828 4594 Acc65I G'GTAC_C Cuts at: 2743 2743 Size: 5422 AclI AA'CG_TT Cuts at: 1563 3549 3922 1563 Size: 1986 373 3063 Fragments arranged by size: 3063 1986 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2401 2675 3485 4869 5137 1632 Size: 769 274 810 1384 268 1917 Fragments arranged by size: 1917 1384 810 769 274 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5225 AflIII A'CryG_T Cuts at: 1203 4321 5298 1203 Size: 3118 977 1327 Fragments arranged by size: 3118 1327 977 AgeI A'CCGG_T Cuts at: 4253 4253 Size: 5422 AhdI GACnn_n'nnGTC Cuts at: 4151 4151 Size: 5422 AleI CACnn'nnGTG Cuts at: 1166 2402 1166 Size: 1236 4186 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5422 AlwNI CAG_nnn'CTG Cuts at: 3165 4737 3165 Size: 1572 3850 ApaLI G'TGCA_C Cuts at: 3479 4635 3479 Size: 1156 4266 ApoI r'AATT_y Cuts at: 1493 2731 2957 3109 5218 1493 Size: 1238 226 152 2109 1697 Fragments arranged by size: 2109 1697 1238 226 152 AseI AT'TA_AT Cuts at: 160 1032 3976 160 Size: 872 2944 1606 Fragments arranged by size: 2944 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 AvaI C'yCGr_G Cuts at: 1870 1943 2122 2162 2190 2747 1870 Size: 73 179 40 28 557 4545 Fragments arranged by size: 4545 557 179 73 40 28 BamHI G'GATC_C Cuts at: 2568 2752 3119 2568 Size: 184 367 4871 Fragments arranged by size: 4871 367 184 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2111 2240 2269 2553 2702 Size: 325 217 627 324 129 29 284 149 Cuts at: 2702 2743 4198 618 Size: 41 1455 1842 Fragments arranged by size: 1842 1455 627 325 324 284 217 149 129 41 29 BanII G_rGCy'C Cuts at: 1391 2415 2741 1391 Size: 1024 326 4072 Fragments arranged by size: 4072 1024 326 BbeI G_GCGC'C Cuts at: 2115 2244 2115 Size: 129 5293 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2663 928 Size: 971 764 3687 Fragments arranged by size: 3687 971 764 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2078 2112 3613 3647 1903 Size: 34 141 34 1501 34 3678 Fragments arranged by size: 3678 1501 141 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3314 4524 3314 Size: 1210 4212 BclI T'GATC_A Cuts at: 1548 1548 Size: 5422 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2304 4033 136 Size: 107 122 71 631 605 632 1729 1525 Fragments arranged by size: 1729 1525 632 631 605 122 107 71 BglII A'GATC_T Cuts at: 5417 5417 Size: 5422 BlpI GC'TnA_GC Cuts at: 2261 2839 2261 Size: 578 4844 Bme1580I G_kGCm'C Cuts at: 1790 2169 3483 4639 1790 Size: 379 1314 1156 2573 Fragments arranged by size: 2573 1314 1156 379 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5422 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2443 3153 4111 Size: 416 53 83 399 24 1026 710 958 Cuts at: 4111 442 Size: 1753 Fragments arranged by size: 1753 1026 958 710 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5422 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4082 1239 Size: 2843 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3547 4412 4710 3547 Size: 865 298 4259 Fragments arranged by size: 4259 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4085 882 Size: 617 2586 2219 Fragments arranged by size: 2586 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3213 493 Size: 711 2009 2702 Fragments arranged by size: 2702 2009 711 BsaBI GATnn'nnATC Cuts at: 5416 5416 Size: 5422 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2112 2241 2369 3229 Size: 53 83 186 936 579 129 128 860 Cuts at: 3229 3611 4246 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 860 635 579 382 186 129 128 83 53 BsaWI w'CCGG_w Cuts at: 3855 4253 4527 4674 5085 3855 Size: 398 274 147 411 4192 Fragments arranged by size: 4192 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2300 2330 1178 Size: 30 165 30 897 30 4270 Fragments arranged by size: 4270 897 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2143 2423 1186 Size: 957 280 4185 Fragments arranged by size: 4185 957 280 BseYI C'CCAG_C Cuts at: 2015 2157 2586 2615 4625 5121 2015 Size: 142 429 29 2010 496 2316 Fragments arranged by size: 2316 2010 496 429 142 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2345 2543 1163 Size: 573 609 198 4042 Fragments arranged by size: 4042 609 573 198 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2202 2872 3633 4661 1540 Size: 275 186 201 670 761 1028 2301 Fragments arranged by size: 2301 1028 761 670 275 201 186 BsiHKAI G_wGCw'C Cuts at: 2415 3483 3568 4639 2415 Size: 1068 85 1071 3198 Fragments arranged by size: 3198 1071 1068 85 BsmI GAATG_Cn' Cuts at: 2933 3026 2933 Size: 93 5329 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2415 2741 3483 3568 4639 1391 Size: 399 379 246 326 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 379 326 246 85 BspEI T'CCGG_A Cuts at: 5085 5085 Size: 5422 BspHI T'CATG_A Cuts at: 3311 5041 3311 Size: 1730 3692 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2565 2777 844 Size: 1721 212 3489 Fragments arranged by size: 3489 1721 212 BsrBI CCG'CTC Cuts at: 1394 1823 3309 1394 Size: 429 1486 3507 Fragments arranged by size: 3507 1486 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3917 4091 65 Size: 1183 2669 174 1396 Fragments arranged by size: 2669 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2303 2550 4066 4253 1700 Size: 110 493 247 1516 187 2869 Fragments arranged by size: 2869 1516 493 247 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5422 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5422 BssSI C'ACGA_G Cuts at: 3482 4494 3482 Size: 1012 4410 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5422 BstBI TT'CG_AA Cuts at: 4226 4226 Size: 5422 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5422 BstXI CCAn_nnnn'nTGG Cuts at: 2404 2404 Size: 5422 BstYI r'GATC_y Cuts at: 1972 2568 2752 3119 3504 3521 4973 5059 5071 Size: 596 184 367 385 17 1452 86 12 Cuts at: 5071 5417 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 596 385 367 346 184 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2247 2397 513 Size: 552 939 243 150 3538 Fragments arranged by size: 3538 939 552 243 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2506 487 Size: 683 56 1280 3403 Fragments arranged by size: 3403 1280 683 56 BtsI GCAGTG_nn' Cuts at: 2938 3732 3752 2938 Size: 794 20 4608 Fragments arranged by size: 4608 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5422 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5387 DraI TTT'AAA Cuts at: 2726 2726 Size: 5422 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4429 809 Size: 507 3113 1802 Fragments arranged by size: 3113 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2869 3758 5387 Size: 54 1425 50 275 18 1039 889 1629 Cuts at: 5387 8 Size: 43 Fragments arranged by size: 1629 1425 1039 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2869 1537 Size: 275 1057 4090 Fragments arranged by size: 4090 1057 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2420 3352 1353 Size: 1067 932 3423 Fragments arranged by size: 3423 1067 932 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2285 4009 4383 4529 387 Size: 1064 138 696 1724 374 146 1280 Fragments arranged by size: 1724 1280 1064 696 374 146 138 EcoICRI GAG'CTC Cuts at: 2413 2413 Size: 5422 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2401 2675 3485 4082 4869 5137 1239 Size: 393 769 274 810 597 787 268 1524 Fragments arranged by size: 1524 810 787 769 597 393 274 268 EcoNI CCTnn'n_nnAGG Cuts at: 2297 2297 Size: 5422 EcoO109I rG'GnC_Cy Cuts at: 2194 2208 2252 2194 Size: 14 44 5364 Fragments arranged by size: 5364 44 14 EcoRI G'AATT_C Cuts at: 2731 3109 2731 Size: 378 5044 FspI TGC'GCA Cuts at: 3928 3928 Size: 5422 HaeII r_GCGC'y Cuts at: 1678 2115 2244 4569 1678 Size: 437 129 2325 2531 Fragments arranged by size: 2531 2325 437 129 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2300 2332 2676 2708 4063 Size: 32 135 32 728 32 344 32 1355 Cuts at: 4063 4095 4137 4169 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1355 728 344 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2491 2766 3012 669 Size: 988 281 553 275 246 3079 Fragments arranged by size: 3079 988 553 281 275 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5422 HpaI GTT'AAC Cuts at: 3012 3012 Size: 5422 KasI G'GCGC_C Cuts at: 2111 2240 2111 Size: 129 5293 KpnI G_GTAC'C Cuts at: 2747 2747 Size: 5422 MfeI C'AATT_G Cuts at: 3021 3021 Size: 5422 MluI A'CGCG_T Cuts at: 5298 5298 Size: 5422 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4536 4720 1305 Size: 3231 184 2007 Fragments arranged by size: 3231 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3600 Fragments arranged by size: 3600 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2402 2477 3380 3739 3898 518 Size: 648 1236 75 903 359 159 2042 Fragments arranged by size: 2042 1236 903 648 359 159 75 MspA1I CmG'CkG Cuts at: 1606 2218 2249 2359 2838 3515 4663 4908 1606 Size: 612 31 110 479 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 612 479 245 110 31 NaeI GCC'GGC Cuts at: 2305 2305 Size: 5422 NarI GG'CG_CC Cuts at: 2112 2241 2112 Size: 129 5293 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3931 Fragments arranged by size: 3931 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5422 NgoMIV G'CCGG_C Cuts at: 2303 2303 Size: 5422 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5422 NotI GC'GGCC_GC Cuts at: 2869 2869 Size: 5422 NspI r_CATG'y Cuts at: 1132 1229 2623 2780 4325 1132 Size: 97 1394 157 1545 2229 Fragments arranged by size: 2229 1545 1394 157 97 PasI CC'CwG_GG Cuts at: 1348 2698 1348 Size: 1350 4072 PciI A'CATG_T Cuts at: 4321 4321 Size: 5422 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5422 PfoI T'CCnGG_A Cuts at: 2571 2571 Size: 5422 PmeI GTTT'AAAC Cuts at: 2726 2726 Size: 5422 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3491 3523 5004 5036 3491 Size: 32 1481 32 3877 Fragments arranged by size: 3877 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2194 2208 2194 Size: 14 5408 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5422 PsiI TTA'TAA Cuts at: 2067 2992 2067 Size: 925 4497 PstI C_TGCA'G Cuts at: 830 2223 2774 830 Size: 1393 551 3478 Fragments arranged by size: 3478 1393 551 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5422 PvuII CAG'CTG Cuts at: 2359 2359 Size: 5422 SacI G_AGCT'C Cuts at: 2415 2415 Size: 5422 SacII CC_GC'GG Cuts at: 2250 2250 Size: 5422 SalI G'TCGA_C Cuts at: 1936 2764 1936 Size: 828 4594 SanDI GG'GwC_CC Cuts at: 2194 2194 Size: 5422 SapI GCTCTTCn'nnn_ Cuts at: 2420 2420 Size: 5422 SbfI CC_TGCA'GG Cuts at: 2774 2774 Size: 5422 ScaI AGT'ACT Cuts at: 3670 5382 3670 Size: 1712 3710 SfcI C'TryA_G Cuts at: 826 1045 2219 2770 3905 4586 4777 826 Size: 219 1174 551 1135 681 191 1471 Fragments arranged by size: 1471 1174 1135 681 551 219 191 SfoI GGC'GCC Cuts at: 2113 2242 2113 Size: 129 5293 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5422 SmaI CCC'GGG Cuts at: 1872 2124 2164 2192 2749 1872 Size: 252 40 28 557 4545 Fragments arranged by size: 4545 557 252 40 28 SmlI C'TyrA_G Cuts at: 820 1017 1943 3526 4427 4689 820 Size: 197 926 1583 901 262 1553 Fragments arranged by size: 1583 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3213 493 Size: 2720 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5422 SphI G_CATG'C Cuts at: 1132 2623 2780 1132 Size: 1491 157 3774 Fragments arranged by size: 3774 1491 157 SrfI GCCC'GGGC Cuts at: 2164 2164 Size: 5422 SspI AAT'ATT Cuts at: 5 52 3346 5 Size: 47 3294 2081 Fragments arranged by size: 3294 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5422 StyI C'CwwG_G Cuts at: 513 1065 2004 2861 513 Size: 552 939 857 3074 Fragments arranged by size: 3074 939 857 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2690 3449 3466 3619 3804 4249 325 Size: 1255 1110 759 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1110 759 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2055 3668 5380 Size: 274 80 33 51 1443 78 1613 1712 Cuts at: 5380 96 Size: 138 Fragments arranged by size: 1712 1613 1443 274 138 80 78 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2161 2193 2265 2297 5382 5414 2161 Size: 32 72 32 3085 32 2169 Fragments arranged by size: 3085 2169 72 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2198 1316 Size: 882 4540 XbaI T'CTAG_A Cuts at: 2758 2758 Size: 5422 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5422 XmaI C'CCGG_G Cuts at: 1870 2122 2162 2190 2747 1870 Size: 252 40 28 557 4545 Fragments arranged by size: 4545 557 252 40 28 XmnI GAAnn'nnTTC Cuts at: 3551 3551 Size: 5422 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3230 276 Size: 53 83 186 936 1696 2468 Fragments arranged by size: 2468 1696 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SexAI SfiI SwaI XcmI