Restriction map for pFN21AB3129
 (Linear) (Six-base) MAPSORT of: KIBB3129.seq  Check: 263  from: 1  to: 717



KIBB3129.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     560     717 
   Size:        560     157
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     396     670     717 
   Size:        396     274      47

  Fragments arranged by size: 

                 396     274      47
 
AleI CACnn'nnGTG

Cuts at:      0     397     717 
   Size:        397     320
 
AvaI C'yCGr_G

Cuts at:      0     117     157     185     717 
   Size:        117      40      28     532

  Fragments arranged by size: 

                 532     117      40      28
 
BamHI G'GATC_C

Cuts at:      0     563     717 
   Size:        563     154
 
BanI G'GyrC_C

Cuts at:      0     106     235     264     548     697     717 
   Size:        106     129      29     284     149      20

  Fragments arranged by size: 

                 284     149     129     106      29      20
 
BanII G_rGCy'C

Cuts at:      0     410     717 
   Size:        410     307
 
BbeI G_GCGC'C

Cuts at:      0     110     239     717 
   Size:        110     129     478

  Fragments arranged by size: 

                 478     129     110
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     658     717 
   Size:        658      59
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      73     107     717 
   Size:         73      34     610

  Fragments arranged by size: 

                 610      73      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     299     717 
   Size:        299     418
 
BlpI GC'TnA_GC

Cuts at:      0     256     717 
   Size:        256     461
 
Bme1580I G_kGCm'C

Cuts at:      0     164     717 
   Size:        164     553
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     438     717 
   Size:        438     279
 
BsaHI Gr'CG_yC

Cuts at:      0     107     236     364     717 
   Size:        107     129     128     353

  Fragments arranged by size: 

                 353     129     128     107
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     295     325     717 
   Size:        295      30     392

  Fragments arranged by size: 

                 392     295      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     138     418     717 
   Size:        138     280     299

  Fragments arranged by size: 

                 299     280     138
 
BseYI C'CCAG_C

Cuts at:      0      10     152     581     610     717 
   Size:         10     142     429      29     107

  Fragments arranged by size: 

                 429     142     107      29      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     538     717 
   Size:        340     198     179

  Fragments arranged by size: 

                 340     198     179
 
BsiEI CG_ry'CG

Cuts at:      0     197     717 
   Size:        197     520
 
BsiHKAI G_wGCw'C

Cuts at:      0     410     717 
   Size:        410     307
 
Bsp1286I G_dGCh'C

Cuts at:      0     164     410     717 
   Size:        164     246     307

  Fragments arranged by size: 

                 307     246     164
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     560     717 
   Size:        560     157
 
BsrFI r'CCGG_y

Cuts at:      0     298     545     717 
   Size:        298     247     172

  Fragments arranged by size: 

                 298     247     172
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     399     717 
   Size:        399     318
 
BstYI r'GATC_y

Cuts at:      0     563     717 
   Size:        563     154
 
BtgI C'CryG_G

Cuts at:      0     242     392     717 
   Size:        242     150     325

  Fragments arranged by size: 

                 325     242     150
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     501     717 
   Size:        501     216
 
EarI CTCTTCn'nnn_

Cuts at:      0     415     717 
   Size:        415     302
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     280     717 
   Size:        280     437
 
EcoICRI GAG'CTC

Cuts at:      0     408     717 
   Size:        408     309
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     396     670     717 
   Size:        396     274      47

  Fragments arranged by size: 

                 396     274      47
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     292     717 
   Size:        292     425
 
EcoO109I rG'GnC_Cy

Cuts at:      0     189     203     247     717 
   Size:        189      14      44     470

  Fragments arranged by size: 

                 470     189      44      14
 
HaeII r_GCGC'y

Cuts at:      0     110     239     717 
   Size:        110     129     478

  Fragments arranged by size: 

                 478     129     110
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     295     327     671     703     717 
   Size:        295      32     344      32      14

  Fragments arranged by size: 

                 344     295      32      32      14
 
HincII GTy'rAC

Cuts at:      0     486     717 
   Size:        486     231
 
KasI G'GCGC_C

Cuts at:      0     106     235     717 
   Size:        106     129     482

  Fragments arranged by size: 

                 482     129     106
 
MslI CAynn'nnrTG

Cuts at:      0     397     472     717 
   Size:        397      75     245

  Fragments arranged by size: 

                 397     245      75
 
MspA1I CmG'CkG

Cuts at:      0     213     244     354     717 
   Size:        213      31     110     363

  Fragments arranged by size: 

                 363     213     110      31
 
NaeI GCC'GGC

Cuts at:      0     300     717 
   Size:        300     417
 
NarI GG'CG_CC

Cuts at:      0     107     236     717 
   Size:        107     129     481

  Fragments arranged by size: 

                 481     129     107
 
NgoMIV G'CCGG_C

Cuts at:      0     298     717 
   Size:        298     419
 
NspI r_CATG'y

Cuts at:      0     618     717 
   Size:        618      99
 
PasI CC'CwG_GG

Cuts at:      0     693     717 
   Size:        693      24
 
PfoI T'CCnGG_A

Cuts at:      0     566     717 
   Size:        566     151
 
PpuMI rG'GwC_Cy

Cuts at:      0     189     203     717 
   Size:        189      14     514

  Fragments arranged by size: 

                 514     189      14
 
PsiI TTA'TAA

Cuts at:      0      62     717 
   Size:         62     655
 
PstI C_TGCA'G

Cuts at:      0     218     717 
   Size:        218     499
 
PvuII CAG'CTG

Cuts at:      0     354     717 
   Size:        354     363
 
SacI G_AGCT'C

Cuts at:      0     410     717 
   Size:        410     307
 
SacII CC_GC'GG

Cuts at:      0     245     717 
   Size:        245     472
 
SanDI GG'GwC_CC

Cuts at:      0     189     717 
   Size:        189     528
 
SapI GCTCTTCn'nnn_

Cuts at:      0     415     717 
   Size:        415     302
 
SfcI C'TryA_G

Cuts at:      0     214     717 
   Size:        214     503
 
SfoI GGC'GCC

Cuts at:      0     108     237     717 
   Size:        108     129     480

  Fragments arranged by size: 

                 480     129     108
 
SmaI CCC'GGG

Cuts at:      0     119     159     187     717 
   Size:        119      40      28     530

  Fragments arranged by size: 

                 530     119      40      28
 
SphI G_CATG'C

Cuts at:      0     618     717 
   Size:        618      99
 
SrfI GCCC'GGGC

Cuts at:      0     159     717 
   Size:        159     558
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     685     717 
   Size:        685      32
 
TatI w'GTAC_w

Cuts at:      0      50     717 
   Size:         50     667
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     156     188     260     292     717 
   Size:        156      32      72      32     425

  Fragments arranged by size: 

                 425     156      72      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     193     717 
   Size:        193     524
 
XmaI C'CCGG_G

Cuts at:      0     117     157     185     717 
   Size:        117      40      28     532

  Fragments arranged by size: 

                 532     117      40      28
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI     BglI     BlpI Bme1580I     BmrI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrFI
    BstXI    BstYI     BtgI    BtgZI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    HaeII    Hin4I   HincII     KasI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NspI     PasI     PfoI    PpuMI     PsiI
     PstI    PvuII     SacI    SacII    SanDI     SapI     SfcI     SfoI
     SmaI     SphI     SrfI    TaqII     TatI     TstI  Tth111I     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BbvCI    BciVI     BclI    BfrBI
    BglII    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SalI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SspI     StuI     StyI     SwaI     XbaI
     XcmI     XhoI     XmnI     ZraI
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