(Circular) (Six-base) MAPSORT of: pFN21AB3109.seq Check: 521 from: 1 to: 10063 pFN21AB3109 10063 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5351 5351 Size: 10063 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3619 7873 278 Size: 53 83 186 936 2083 4254 2468 Fragments arranged by size: 4254 2468 2083 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3526 7406 1937 Size: 1589 3880 4594 Fragments arranged by size: 4594 3880 1589 Acc65I G'GTAC_C Cuts at: 7384 7384 Size: 10063 AclI AA'CG_TT Cuts at: 1563 8190 8563 1563 Size: 6627 373 3063 Fragments arranged by size: 6627 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2628 2997 6393 6423 6462 6671 7152 8126 Size: 996 369 3396 30 39 209 481 974 Cuts at: 8126 9510 9778 1632 Size: 1384 268 1917 Fragments arranged by size: 3396 1917 1384 996 974 481 369 268 209 39 30 AflII C'TTAA_G Cuts at: 820 1017 2327 3502 6977 820 Size: 197 1310 1175 3475 3906 Fragments arranged by size: 3906 3475 1310 1175 197 AflIII A'CryG_T Cuts at: 1203 3200 6933 8962 9939 1203 Size: 1997 3733 2029 977 1327 Fragments arranged by size: 3733 2029 1997 1327 977 AgeI A'CCGG_T Cuts at: 8894 8894 Size: 10063 AhdI GACnn_n'nnGTC Cuts at: 2433 8792 2433 Size: 6359 3704 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 10063 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 6714 1184 Size: 5530 4533 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2063 2095 6911 6943 2063 Size: 32 4816 32 5183 Fragments arranged by size: 5183 4816 32 32 AlwNI CAG_nnn'CTG Cuts at: 3484 4194 4643 4800 6182 6880 6914 7806 9378 Size: 710 449 157 1382 698 34 892 1572 Cuts at: 9378 3484 Size: 4169 Fragments arranged by size: 4169 1572 1382 892 710 698 449 157 34 ApaLI G'TGCA_C Cuts at: 4422 5497 8120 9276 4422 Size: 1075 2623 1156 5209 Fragments arranged by size: 5209 2623 1156 1075 ApoI r'AATT_y Cuts at: 1493 2393 2680 3231 5924 7111 7372 7598 7750 Size: 900 287 551 2693 1187 261 226 152 Cuts at: 7750 9859 1493 Size: 2109 1697 Fragments arranged by size: 2693 2109 1697 1187 900 551 287 261 226 152 AseI AT'TA_AT Cuts at: 160 1032 8617 160 Size: 872 7585 1606 Fragments arranged by size: 7585 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10063 AvaI C'yCGr_G Cuts at: 1870 1943 2055 2075 5512 7388 1870 Size: 73 112 20 3437 1876 4545 Fragments arranged by size: 4545 3437 1876 112 73 20 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4204 4237 4843 4876 5041 5074 4204 Size: 33 606 33 165 33 9193 Fragments arranged by size: 9193 606 165 33 33 33 BamHI G'GATC_C Cuts at: 7393 7760 7393 Size: 367 9696 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5105 5951 7384 8839 618 Size: 325 217 627 3318 846 1433 1455 1842 Fragments arranged by size: 3318 1842 1455 1433 846 627 325 217 BanII G_rGCy'C Cuts at: 1391 2056 4094 5717 7382 1391 Size: 665 2038 1623 1665 4072 Fragments arranged by size: 4072 2038 1665 1623 665 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5165 5512 6081 6450 6464 6864 7073 Size: 971 3266 347 569 369 14 400 209 Cuts at: 7073 7254 928 Size: 181 3737 Fragments arranged by size: 3737 3266 971 569 400 369 347 209 181 14 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2796 2830 4541 4575 5060 5094 8254 Size: 34 859 34 1711 34 485 34 3160 Cuts at: 8254 8288 1903 Size: 34 3678 Fragments arranged by size: 3678 3160 1711 859 485 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2667 4844 4880 5042 7955 9165 2667 Size: 2177 36 162 2913 1210 3565 Fragments arranged by size: 3565 2913 2177 1210 162 36 BclI T'GATC_A Cuts at: 1548 5568 1548 Size: 4020 6043 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 8674 136 Size: 107 122 71 631 605 7002 1525 Fragments arranged by size: 7002 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 10058 10058 Size: 10063 BlpI GC'TnA_GC Cuts at: 7480 7480 Size: 10063 Bme1580I G_kGCm'C Cuts at: 1790 2848 4426 4578 5108 5501 6367 8124 9280 Size: 1058 1578 152 530 393 866 1757 1156 Cuts at: 9280 1790 Size: 2573 Fragments arranged by size: 2573 1757 1578 1156 1058 866 530 393 152 BmgBI CAC'GTC Cuts at: 1318 2169 4084 5383 6079 1318 Size: 851 1915 1299 696 5302 Fragments arranged by size: 5302 1915 1299 851 696 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3505 3883 4443 Size: 416 53 83 399 24 2088 378 560 Cuts at: 4443 4588 4631 4653 4995 6094 7794 8752 442 Size: 145 43 22 342 1099 1700 958 1753 Fragments arranged by size: 2088 1753 1700 1099 958 560 416 399 378 342 145 83 53 43 24 22 BmtI G_CTAG'C Cuts at: 1056 2027 1056 Size: 971 9092 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2000 3426 3777 5627 8723 1239 Size: 761 1426 351 1850 3096 2579 Fragments arranged by size: 3096 2579 1850 1426 761 351 Bpu10I CC'TnA_GC Cuts at: 2067 2067 Size: 10063 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2722 3970 4982 5784 6011 6654 7182 7263 8188 Size: 1248 1012 802 227 643 528 81 925 Cuts at: 8188 9053 9351 2722 Size: 865 298 3434 Fragments arranged by size: 3434 1248 1012 925 865 802 643 528 298 227 81 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2258 2962 3269 5367 6403 8726 882 Size: 617 759 704 307 2098 1036 2323 2219 Fragments arranged by size: 2323 2219 2098 1036 759 704 617 307 BsaAI yAC'GTr Cuts at: 493 1204 3201 6647 7854 493 Size: 711 1997 3446 1207 2702 Fragments arranged by size: 3446 2702 1997 1207 711 BsaBI GATnn'nnATC Cuts at: 2256 2762 10057 2256 Size: 506 7295 2262 Fragments arranged by size: 7295 2262 506 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3616 4783 5426 7870 Size: 53 83 186 936 2083 1167 643 2444 Cuts at: 7870 8252 8887 275 Size: 382 635 1451 Fragments arranged by size: 2444 2083 1451 1167 936 643 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3738 8496 8894 9168 9315 9726 3738 Size: 4758 398 274 147 411 4075 Fragments arranged by size: 4758 4075 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2299 2329 4161 4191 1178 Size: 30 165 30 896 30 1832 30 7050 Fragments arranged by size: 7050 1832 896 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4060 6014 6134 6512 1186 Size: 2874 1954 120 378 4737 Fragments arranged by size: 4737 2874 1954 378 120 BseYI C'CCAG_C Cuts at: 3208 6939 9266 9762 3208 Size: 3731 2327 496 3509 Fragments arranged by size: 3731 3509 2327 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2068 2162 2923 3917 5515 7071 1163 Size: 573 332 94 761 994 1598 1556 4155 Fragments arranged by size: 4155 1598 1556 994 761 573 332 94 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2140 2462 4010 7513 8274 9302 Size: 275 186 139 322 1548 3503 761 1028 Cuts at: 9302 1540 Size: 2301 Fragments arranged by size: 3503 2301 1548 1028 761 322 275 186 139 BsiHKAI G_wGCw'C Cuts at: 2146 3495 3593 4426 5121 5501 8124 8209 9280 Size: 1349 98 833 695 380 2623 85 1071 Cuts at: 9280 2146 Size: 2929 Fragments arranged by size: 2929 2623 1349 1071 833 695 380 98 85 BsmI GAATG_Cn' Cuts at: 4331 6016 7574 7667 4331 Size: 1685 1558 93 6727 Fragments arranged by size: 6727 1685 1558 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5417 5417 Size: 10063 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2056 2146 2848 3495 3593 4094 4426 Size: 399 266 90 702 647 98 501 332 Cuts at: 4426 4578 5108 5121 5501 5717 6367 7382 8124 Size: 152 530 13 380 216 650 1015 742 Cuts at: 8124 8209 9280 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1015 742 702 650 647 530 501 399 380 332 266 216 152 98 90 85 13 BspEI T'CCGG_A Cuts at: 9726 9726 Size: 10063 BspHI T'CATG_A Cuts at: 2260 6057 7952 9682 2260 Size: 3797 1895 1730 2641 Fragments arranged by size: 3797 2641 1895 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2980 4418 5351 6888 7418 844 Size: 2136 1438 933 1537 530 3489 Fragments arranged by size: 3489 2136 1537 1438 933 530 BsrBI CCG'CTC Cuts at: 1394 1823 3721 7950 1394 Size: 429 1898 4229 3507 Fragments arranged by size: 4229 3507 1898 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3022 3267 3514 4618 5269 5299 5571 Size: 1183 1774 245 247 1104 651 30 272 Cuts at: 5571 6499 6705 8558 8732 65 Size: 928 206 1853 174 1396 Fragments arranged by size: 1853 1774 1396 1183 1104 928 651 272 247 245 206 174 30 BsrFI r'CCGG_y Cuts at: 1700 1810 3597 3761 4009 4386 5040 8707 8894 Size: 110 1787 164 248 377 654 3667 187 Cuts at: 8894 1700 Size: 2869 Fragments arranged by size: 3667 2869 1787 654 377 248 187 164 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10063 BssHII G'CGCG_C Cuts at: 1925 2062 1925 Size: 137 9926 BssSI C'ACGA_G Cuts at: 8123 9135 8123 Size: 1012 9051 BstAPI GCAn_nnn'nTGC Cuts at: 794 5352 5538 794 Size: 4558 186 5319 Fragments arranged by size: 5319 4558 186 BstBI TT'CG_AA Cuts at: 8867 8867 Size: 10063 BstEII G'GTnAC_C Cuts at: 1184 2158 4180 1184 Size: 974 2022 7067 Fragments arranged by size: 7067 2022 974 BstXI CCAn_nnnn'nTGG Cuts at: 3495 4613 5857 6343 3495 Size: 1118 1244 486 7215 Fragments arranged by size: 7215 1244 1118 486 BstYI r'GATC_y Cuts at: 1972 2251 2635 2656 3175 3355 4484 4810 7393 Size: 279 384 21 519 180 1129 326 2583 Cuts at: 7393 7760 8145 8162 9614 9700 9712 10058 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 2583 1977 1452 1129 519 385 384 367 346 326 279 180 86 21 17 12 Bsu36I CC'TnA_GG Cuts at: 4335 4882 4335 Size: 547 9516 BtgI C'CryG_G Cuts at: 513 1065 2004 2118 3063 3111 3825 4155 4674 Size: 552 939 114 945 48 714 330 519 Cuts at: 4674 5445 6336 513 Size: 771 891 4240 Fragments arranged by size: 4240 945 939 891 771 714 552 519 330 114 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9324 Fragments arranged by size: 9324 683 56 BtsI GCAGTG_nn' Cuts at: 2893 3137 3355 3436 4559 4741 5315 6774 7579 Size: 244 218 81 1123 182 574 1459 805 Cuts at: 7579 8373 8393 2893 Size: 794 20 4563 Fragments arranged by size: 4563 1459 1123 805 794 574 244 218 182 81 20 ClaI AT'CG_AT Cuts at: 1554 4107 1554 Size: 2553 7510 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10028 DraI TTT'AAA Cuts at: 7367 7367 Size: 10063 DraIII CAC_nnn'GTG Cuts at: 5435 5489 5435 Size: 54 10009 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6353 9070 809 Size: 507 5037 2717 1802 Fragments arranged by size: 5037 2717 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2137 3060 3274 Size: 54 1425 50 275 18 307 923 214 Cuts at: 3274 4007 4516 4615 5229 7510 8399 10028 8 Size: 733 509 99 614 2281 889 1629 43 Fragments arranged by size: 2281 1629 1425 923 889 733 614 509 307 275 214 99 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2137 4007 7510 1537 Size: 275 325 1870 3503 4090 Fragments arranged by size: 4090 3503 1870 325 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5369 7128 7993 1353 Size: 4016 1759 865 3423 Fragments arranged by size: 4016 3423 1759 865 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8650 9024 9170 387 Size: 1064 138 7061 374 146 1280 Fragments arranged by size: 7061 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2000 2628 2997 3426 3777 5627 6393 Size: 393 368 628 369 429 351 1850 766 Cuts at: 6393 6423 6462 6671 7152 8126 8723 9510 9778 Size: 30 39 209 481 974 597 787 268 Cuts at: 9778 1239 Size: 1524 Fragments arranged by size: 1850 1524 974 787 766 628 597 481 429 393 369 368 351 268 209 39 30 EcoNI CCTnn'n_nnAGG Cuts at: 2976 5069 2976 Size: 2093 7970 EcoO109I rG'GnC_Cy Cuts at: 3698 6213 6284 3698 Size: 2515 71 7477 Fragments arranged by size: 7477 2515 71 EcoRI G'AATT_C Cuts at: 2393 7372 7750 2393 Size: 4979 378 4706 Fragments arranged by size: 4979 4706 378 FspI TGC'GCA Cuts at: 2095 8569 2095 Size: 6474 3589 HaeII r_GCGC'y Cuts at: 1678 9210 1678 Size: 7532 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2257 2289 2419 2451 3601 Size: 32 135 32 685 32 130 32 1150 Cuts at: 3601 3633 3981 4013 4096 4128 4865 4897 6080 Size: 32 348 32 83 32 737 32 1183 Cuts at: 6080 6112 8704 8736 8778 8810 1373 Size: 32 2592 32 42 32 2626 Fragments arranged by size: 2626 2592 1183 1150 737 685 348 135 130 83 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3527 7407 7653 669 Size: 988 281 1589 3880 246 3079 Fragments arranged by size: 3880 3079 1589 988 281 246 HindIII A'AGCT_T Cuts at: 748 2150 748 Size: 1402 8661 HpaI GTT'AAC Cuts at: 7653 7653 Size: 10063 KpnI G_GTAC'C Cuts at: 7388 7388 Size: 10063 MfeI C'AATT_G Cuts at: 7662 7662 Size: 10063 MluI A'CGCG_T Cuts at: 6933 9939 6933 Size: 3006 7057 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2398 3941 4029 4381 9177 9361 1305 Size: 1093 1543 88 352 4796 184 2007 Fragments arranged by size: 4796 2007 1543 1093 352 184 88 MscI TGG'CCA Cuts at: 10 64 1489 1832 3062 3276 4518 4617 5231 Size: 54 1425 343 1230 214 1242 99 614 Cuts at: 5231 10 Size: 4842 Fragments arranged by size: 4842 1425 1242 1230 614 343 214 99 54 MslI CAynn'nnrTG Cuts at: 518 1166 3315 3893 4623 4686 8021 8380 8539 Size: 648 2149 578 730 63 3335 359 159 Cuts at: 8539 518 Size: 2042 Fragments arranged by size: 3335 2149 2042 730 648 578 359 159 63 MspA1I CmG'CkG Cuts at: 1606 2106 2441 3078 3212 5205 5227 6182 7142 Size: 500 335 637 134 1993 22 955 960 Cuts at: 7142 7479 8156 9304 9549 1606 Size: 337 677 1148 245 2120 Fragments arranged by size: 2120 1993 1148 960 955 677 637 500 337 335 245 134 22 NaeI GCC'GGC Cuts at: 3599 3763 4388 3599 Size: 164 625 9274 Fragments arranged by size: 9274 625 164 NcoI C'CATG_G Cuts at: 513 1065 2004 3111 3825 4674 6336 513 Size: 552 939 1107 714 849 1662 4240 Fragments arranged by size: 4240 1662 1107 939 849 714 552 NdeI CA'TA_TG Cuts at: 387 2237 387 Size: 1850 8213 NgoMIV G'CCGG_C Cuts at: 3597 3761 4386 3597 Size: 164 625 9274 Fragments arranged by size: 9274 625 164 NheI G'CTAG_C Cuts at: 1052 2023 1052 Size: 971 9092 NotI GC'GGCC_GC Cuts at: 7510 7510 Size: 10063 NspI r_CATG'y Cuts at: 1132 1229 2902 5323 7359 7421 8966 1132 Size: 97 1673 2421 2036 62 1545 2229 Fragments arranged by size: 2421 2229 2036 1673 1545 97 62 PasI CC'CwG_GG Cuts at: 1348 5412 6209 1348 Size: 4064 797 5202 Fragments arranged by size: 5202 4064 797 PciI A'CATG_T Cuts at: 8962 8962 Size: 10063 PflMI CCAn_nnn'nTGG Cuts at: 1108 4643 5220 1108 Size: 3535 577 5951 Fragments arranged by size: 5951 3535 577 PfoI T'CCnGG_A Cuts at: 2753 3403 3732 3749 4846 5604 2753 Size: 650 329 17 1097 758 7212 Fragments arranged by size: 7212 1097 758 650 329 17 PmeI GTTT'AAAC Cuts at: 7367 7367 Size: 10063 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8132 8164 9645 9677 8132 Size: 32 1481 32 8518 Fragments arranged by size: 8518 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10063 PsiI TTA'TAA Cuts at: 7633 7633 Size: 10063 PstI C_TGCA'G Cuts at: 830 5348 6254 6781 7415 830 Size: 4518 906 527 634 3478 Fragments arranged by size: 4518 3478 906 634 527 PvuI CG_AT'CG Cuts at: 2001 2462 2001 Size: 461 9602 PvuII CAG'CTG Cuts at: 2106 2441 3212 5227 7142 2106 Size: 335 771 2015 1915 5027 Fragments arranged by size: 5027 2015 1915 771 335 SalI G'TCGA_C Cuts at: 1936 3525 7405 1936 Size: 1589 3880 4594 Fragments arranged by size: 4594 3880 1589 SbfI CC_TGCA'GG Cuts at: 7415 7415 Size: 10063 ScaI AGT'ACT Cuts at: 8311 10023 8311 Size: 1712 8351 SexAI A'CCwGG_T Cuts at: 3637 3960 3637 Size: 323 9740 SfcI C'TryA_G Cuts at: 826 1045 5344 5867 6250 6777 7411 8546 9227 Size: 219 4299 523 383 527 634 1135 681 Cuts at: 9227 9418 826 Size: 191 1471 Fragments arranged by size: 4299 1471 1135 681 634 527 523 383 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10063 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10063 SmaI CCC'GGG Cuts at: 1872 7390 1872 Size: 5518 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2327 2701 3502 3985 4997 5799 Size: 197 926 384 374 801 483 1012 802 Cuts at: 5799 5990 6669 6977 7197 7278 8167 9068 9330 Size: 191 679 308 220 81 889 901 262 Cuts at: 9330 820 Size: 1553 Fragments arranged by size: 1553 1012 926 901 889 802 801 679 483 384 374 308 262 220 197 191 81 SnaBI TAC'GTA Cuts at: 493 7854 493 Size: 7361 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10063 SphI G_CATG'C Cuts at: 1132 5323 7421 1132 Size: 4191 2098 3774 Fragments arranged by size: 4191 3774 2098 SspI AAT'ATT Cuts at: 5 52 7987 5 Size: 47 7935 2081 Fragments arranged by size: 7935 2081 47 StuI AGG'CCT Cuts at: 1517 2519 4314 5621 1517 Size: 1002 1795 1307 5959 Fragments arranged by size: 5959 1795 1307 1002 StyI C'CwwG_G Cuts at: 513 1065 2004 2669 2916 2963 3111 3544 3825 Size: 552 939 665 247 47 148 433 281 Cuts at: 3825 4674 5616 5955 6336 7502 513 Size: 849 942 339 381 1166 3074 Fragments arranged by size: 3074 1166 942 939 849 665 552 433 381 339 281 247 148 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3171 8090 8107 8260 8445 8890 325 Size: 1255 1591 4919 17 153 185 445 1498 Fragments arranged by size: 4919 1591 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4062 8309 10021 Size: 274 80 33 51 1443 2085 4247 1712 Cuts at: 10021 96 Size: 138 Fragments arranged by size: 4247 2085 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4161 4193 5586 5618 10023 10055 4161 Size: 32 1393 32 4405 32 4169 Fragments arranged by size: 4405 4169 1393 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10063 XbaI T'CTAG_A Cuts at: 4222 7399 4222 Size: 3177 6886 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5321 6037 6478 5321 Size: 716 441 8906 Fragments arranged by size: 8906 716 441 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10063 XmaI C'CCGG_G Cuts at: 1870 7388 1870 Size: 5518 4545 XmnI GAAnn'nnTTC Cuts at: 4203 6447 6861 8192 4203 Size: 2244 414 1331 6074 Fragments arranged by size: 6074 2244 1331 414 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3617 7871 276 Size: 53 83 186 936 2083 4254 2468 Fragments arranged by size: 4254 2468 2083 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI