(Circular) (Six-base) MAPSORT of: pFN21AB3104.seq Check: 4015 from: 1 to: 7114 pFN21AB3104 7114 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4028 4028 Size: 7114 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4924 278 Size: 53 83 186 936 3388 2468 Fragments arranged by size: 3388 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2462 4457 1937 Size: 525 1995 4594 Fragments arranged by size: 4594 1995 525 Acc65I G'GTAC_C Cuts at: 4435 4435 Size: 7114 AclI AA'CG_TT Cuts at: 1563 5241 5614 1563 Size: 3678 373 3063 Fragments arranged by size: 3678 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2212 2528 3418 3430 3671 3926 4342 5177 Size: 580 316 890 12 241 255 416 835 Cuts at: 5177 6561 6829 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 890 835 580 416 316 268 255 241 12 AfeI AGC'GCT Cuts at: 2029 3439 2029 Size: 1410 5704 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6917 AflIII A'CryG_T Cuts at: 1203 2617 2887 3030 3559 6013 6990 1203 Size: 1414 270 143 529 2454 977 1327 Fragments arranged by size: 2454 1414 1327 977 529 270 143 AgeI A'CCGG_T Cuts at: 4326 5945 4326 Size: 1619 5495 AhdI GACnn_n'nnGTC Cuts at: 5843 5843 Size: 7114 AleI CACnn'nnGTG Cuts at: 1166 2731 2972 1166 Size: 1565 241 5308 Fragments arranged by size: 5308 1565 241 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7114 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4152 4184 4152 Size: 32 7082 AlwNI CAG_nnn'CTG Cuts at: 2065 2688 3649 4171 4321 4857 6429 2065 Size: 623 961 522 150 536 1572 2750 Fragments arranged by size: 2750 1572 961 623 536 522 150 ApaI G_GGCC'C Cuts at: 2564 2564 Size: 7114 ApaLI G'TGCA_C Cuts at: 2613 3509 5171 6327 2613 Size: 896 1662 1156 3400 Fragments arranged by size: 3400 1662 1156 896 ApoI r'AATT_y Cuts at: 1493 2244 2282 2487 3194 4200 4423 4649 4801 Size: 751 38 205 707 1006 223 226 152 Cuts at: 4801 6910 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1006 751 707 226 223 205 152 38 AseI AT'TA_AT Cuts at: 160 1032 5668 160 Size: 872 4636 1606 Fragments arranged by size: 4636 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7114 AvaI C'yCGr_G Cuts at: 1870 1943 2255 3712 4439 1870 Size: 73 312 1457 727 4545 Fragments arranged by size: 4545 1457 727 312 73 BamHI G'GATC_C Cuts at: 2500 4362 4444 4811 2500 Size: 1862 82 367 4803 Fragments arranged by size: 4803 1862 367 82 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3707 4435 5890 618 Size: 325 217 627 1920 728 1455 1842 Fragments arranged by size: 1920 1842 1455 728 627 325 217 BanII G_rGCy'C Cuts at: 1391 2564 2802 4433 1391 Size: 1173 238 1631 4072 Fragments arranged by size: 4072 1631 1173 238 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2241 2720 928 Size: 971 342 479 5322 Fragments arranged by size: 5322 971 479 342 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2737 2771 2980 3014 5305 5339 1903 Size: 34 800 34 209 34 2291 34 3678 Fragments arranged by size: 3678 2291 800 209 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5006 6216 5006 Size: 1210 5904 BclI T'GATC_A Cuts at: 1548 4173 1548 Size: 2625 4489 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2568 5725 136 Size: 107 122 71 631 605 896 3157 1525 Fragments arranged by size: 3157 1525 896 631 605 122 107 71 BglII A'GATC_T Cuts at: 2221 3315 7109 2221 Size: 1094 3794 2226 Fragments arranged by size: 3794 2226 1094 BlpI GC'TnA_GC Cuts at: 4531 4531 Size: 7114 Bme1580I G_kGCm'C Cuts at: 1790 2564 2617 3513 5175 6331 1790 Size: 774 53 896 1662 1156 2573 Fragments arranged by size: 2573 1662 1156 896 774 53 BmgBI CAC'GTC Cuts at: 1318 3277 1318 Size: 1959 5155 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3280 4845 5803 Size: 416 53 83 399 24 1863 1565 958 Cuts at: 5803 442 Size: 1753 Fragments arranged by size: 1863 1753 1565 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7114 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2097 2097 Size: 7114 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2388 2942 3887 4288 5774 1239 Size: 1149 554 945 401 1486 2579 Fragments arranged by size: 2579 1486 1149 945 554 401 Bpu10I CC'TnA_GC Cuts at: 2480 3326 3968 2480 Size: 846 642 5626 Fragments arranged by size: 5626 846 642 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3458 5239 6104 6402 3458 Size: 1781 865 298 4170 Fragments arranged by size: 4170 1781 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2650 2742 5777 882 Size: 617 1151 92 3035 2219 Fragments arranged by size: 3035 2219 1151 617 92 BsaAI yAC'GTr Cuts at: 493 1204 4905 493 Size: 711 3701 2702 Fragments arranged by size: 3701 2702 711 BsaBI GATnn'nnATC Cuts at: 7108 7108 Size: 7114 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3054 4921 5303 5938 Size: 53 83 186 936 1521 1867 382 635 Cuts at: 5938 275 Size: 1451 Fragments arranged by size: 1867 1521 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2740 4326 5547 5945 6219 6366 6777 2740 Size: 1586 1221 398 274 147 411 3077 Fragments arranged by size: 3077 1586 1221 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2801 2831 1178 Size: 30 165 30 1398 30 5461 Fragments arranged by size: 5461 1398 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2717 3884 4418 4421 1186 Size: 1531 1167 534 3 3879 Fragments arranged by size: 3879 1531 1167 534 3 BseYI C'CCAG_C Cuts at: 2873 3222 3750 3856 3985 6317 6813 2873 Size: 349 528 106 129 2332 496 3174 Fragments arranged by size: 3174 2332 528 496 349 129 106 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2066 3055 3409 3821 1163 Size: 573 330 989 354 412 4456 Fragments arranged by size: 4456 989 573 412 354 330 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3805 4564 5325 6353 1540 Size: 275 186 1804 759 761 1028 2301 Fragments arranged by size: 2301 1804 1028 761 759 275 186 BsiHKAI G_wGCw'C Cuts at: 2617 2802 3332 3513 3974 5175 5260 6331 2617 Size: 185 530 181 461 1201 85 1071 3400 Fragments arranged by size: 3400 1201 1071 530 461 185 181 85 BsmI GAATG_Cn' Cuts at: 4358 4625 4718 4358 Size: 267 93 6754 Fragments arranged by size: 6754 267 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3343 3749 3343 Size: 406 6708 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2564 2617 2802 3332 3513 3974 4433 Size: 399 774 53 185 530 181 461 459 Cuts at: 4433 5175 5260 6331 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 774 742 530 461 459 399 185 181 85 53 BspEI T'CCGG_A Cuts at: 2740 6777 2740 Size: 4037 3077 BspHI T'CATG_A Cuts at: 2248 5003 6733 2248 Size: 2755 1730 2629 Fragments arranged by size: 2755 2629 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3348 3642 4028 4469 844 Size: 2504 294 386 441 3489 Fragments arranged by size: 3489 2504 441 386 294 BsrBI CCG'CTC Cuts at: 1394 1823 5001 1394 Size: 429 3178 3507 Fragments arranged by size: 3507 3178 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2539 5609 5783 65 Size: 1183 1291 3070 174 1396 Fragments arranged by size: 3070 1396 1291 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2813 4326 5758 5945 1700 Size: 110 1003 1513 1432 187 2869 Fragments arranged by size: 2869 1513 1432 1003 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7114 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7114 BssSI C'ACGA_G Cuts at: 2749 3083 5174 6186 2749 Size: 334 2091 1012 3677 Fragments arranged by size: 3677 2091 1012 334 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7114 BstBI TT'CG_AA Cuts at: 5918 5918 Size: 7114 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7114 BstXI CCAn_nnnn'nTGG Cuts at: 2641 3376 2641 Size: 735 6379 BstYI r'GATC_y Cuts at: 1972 2221 2500 3285 3315 4362 4444 4811 5196 Size: 249 279 785 30 1047 82 367 385 Cuts at: 5196 5213 6665 6751 6763 7109 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1047 785 385 367 346 279 249 86 82 30 17 12 BstZ17I GTA'TAC Cuts at: 2463 2463 Size: 7114 BtgI C'CryG_G Cuts at: 513 1065 2945 2970 3060 3775 3805 4096 513 Size: 552 1880 25 90 715 30 291 3531 Fragments arranged by size: 3531 1880 715 552 291 90 30 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2707 487 Size: 683 56 1481 4894 Fragments arranged by size: 4894 1481 683 56 BtsI GCAGTG_nn' Cuts at: 2114 3401 4185 4630 5424 5444 2114 Size: 1287 784 445 794 20 3784 Fragments arranged by size: 3784 1287 794 784 445 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7114 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7079 DraI TTT'AAA Cuts at: 4418 4418 Size: 7114 DraIII CAC_nnn'GTG Cuts at: 3844 3844 Size: 7114 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6121 809 Size: 507 4805 1802 Fragments arranged by size: 4805 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3145 3802 3936 Size: 54 1425 50 275 18 1315 657 134 Cuts at: 3936 4561 5450 7079 8 Size: 625 889 1629 43 Fragments arranged by size: 1629 1425 1315 889 657 625 275 134 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3802 4561 1537 Size: 275 1990 759 4090 Fragments arranged by size: 4090 1990 759 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2231 2694 5044 1353 Size: 878 463 2350 3423 Fragments arranged by size: 3423 2350 878 463 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5701 6075 6221 387 Size: 1064 138 4112 374 146 1280 Fragments arranged by size: 4112 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2800 2800 Size: 7114 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2212 2388 2528 2942 3418 3430 3671 Size: 393 580 176 140 414 476 12 241 Cuts at: 3671 3887 3926 4288 4342 5177 5774 6561 6829 Size: 216 39 362 54 835 597 787 268 Cuts at: 6829 1239 Size: 1524 Fragments arranged by size: 1524 835 787 597 580 476 414 393 362 268 241 216 176 140 54 39 12 EcoO109I rG'GnC_Cy Cuts at: 2560 2561 3337 3485 2560 Size: 1 776 148 6189 Fragments arranged by size: 6189 776 148 1 EcoRI G'AATT_C Cuts at: 2282 4423 4801 2282 Size: 2141 378 4595 Fragments arranged by size: 4595 2141 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3175 3337 3175 Size: 162 6952 FspI TGC'GCA Cuts at: 5620 5620 Size: 7114 HaeII r_GCGC'y Cuts at: 1678 2031 3441 6261 1678 Size: 353 1410 2820 2531 Fragments arranged by size: 2820 2531 1410 353 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2460 2492 3342 3374 4165 Size: 32 135 32 888 32 850 32 791 Cuts at: 4165 4197 5755 5787 5829 5861 1373 Size: 32 1558 32 42 32 2626 Fragments arranged by size: 2626 1558 888 850 791 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4458 4704 669 Size: 988 281 2520 246 3079 Fragments arranged by size: 3079 2520 988 281 246 HindIII A'AGCT_T Cuts at: 748 2540 748 Size: 1792 5322 HpaI GTT'AAC Cuts at: 4704 4704 Size: 7114 KpnI G_GTAC'C Cuts at: 4439 4439 Size: 7114 MfeI C'AATT_G Cuts at: 2109 4713 2109 Size: 2604 4510 MluI A'CGCG_T Cuts at: 6990 6990 Size: 7114 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6228 6412 1305 Size: 4923 184 2007 Fragments arranged by size: 4923 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5292 Fragments arranged by size: 5292 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2639 2731 2969 2972 3087 3209 5072 Size: 648 1473 92 238 3 115 122 1863 Cuts at: 5072 5431 5590 518 Size: 359 159 2042 Fragments arranged by size: 2042 1863 1473 648 359 238 159 122 115 92 3 MspA1I CmG'CkG Cuts at: 1606 2177 2578 3813 4530 5207 6355 6600 1606 Size: 571 401 1235 717 677 1148 245 2120 Fragments arranged by size: 2120 1235 1148 717 677 571 401 245 NcoI C'CATG_G Cuts at: 513 1065 2970 4096 513 Size: 552 1905 1126 3531 Fragments arranged by size: 3531 1905 1126 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7114 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7114 NotI GC'GGCC_GC Cuts at: 4561 4561 Size: 7114 NspI r_CATG'y Cuts at: 1132 1229 2621 3563 4472 6017 1132 Size: 97 1392 942 909 1545 2229 Fragments arranged by size: 2229 1545 1392 942 909 97 PasI CC'CwG_GG Cuts at: 1348 3178 1348 Size: 1830 5284 PciI A'CATG_T Cuts at: 2617 3559 6013 2617 Size: 942 2454 3718 Fragments arranged by size: 3718 2454 942 PflMI CCAn_nnn'nTGG Cuts at: 1108 2920 3457 1108 Size: 1812 537 4765 Fragments arranged by size: 4765 1812 537 PfoI T'CCnGG_A Cuts at: 4365 4365 Size: 7114 PmeI GTTT'AAAC Cuts at: 4418 4418 Size: 7114 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5183 5215 6696 6728 5183 Size: 32 1481 32 5569 Fragments arranged by size: 5569 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3337 3485 3337 Size: 148 6966 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7114 PsiI TTA'TAA Cuts at: 4684 4684 Size: 7114 PspOMI G'GGCC_C Cuts at: 2560 2560 Size: 7114 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3413 3445 3413 Size: 32 7082 PstI C_TGCA'G Cuts at: 830 4466 830 Size: 3636 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7114 PvuII CAG'CTG Cuts at: 2578 2578 Size: 7114 SacI G_AGCT'C Cuts at: 2802 2802 Size: 7114 SalI G'TCGA_C Cuts at: 1936 4456 1936 Size: 2520 4594 SbfI CC_TGCA'GG Cuts at: 4466 4466 Size: 7114 ScaI AGT'ACT Cuts at: 2960 5362 7074 2960 Size: 2402 1712 3000 Fragments arranged by size: 3000 2402 1712 SexAI A'CCwGG_T Cuts at: 3411 3411 Size: 7114 SfcI C'TryA_G Cuts at: 826 1045 2214 2290 3361 4462 5597 6278 6469 Size: 219 1169 76 1071 1101 1135 681 191 Cuts at: 6469 826 Size: 1471 Fragments arranged by size: 1471 1169 1135 1101 1071 681 219 191 76 SfiI GGCCn_nnn'nGGCC Cuts at: 2568 2568 Size: 7114 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7114 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7114 SmaI CCC'GGG Cuts at: 1872 3714 4441 1872 Size: 1842 727 4545 Fragments arranged by size: 4545 1842 727 SmlI C'TyrA_G Cuts at: 820 1017 1943 3473 5218 6119 6381 820 Size: 197 926 1530 1745 901 262 1553 Fragments arranged by size: 1745 1553 1530 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4905 493 Size: 4412 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7114 SphI G_CATG'C Cuts at: 1132 4472 1132 Size: 3340 3774 SspI AAT'ATT Cuts at: 5 52 5038 5 Size: 47 4986 2081 Fragments arranged by size: 4986 2081 47 StuI AGG'CCT Cuts at: 1517 3101 1517 Size: 1584 5530 StyI C'CwwG_G Cuts at: 513 1065 2016 2853 2970 2994 3138 3820 4096 Size: 552 951 837 117 24 144 682 276 Cuts at: 4096 4128 4553 513 Size: 32 425 3074 Fragments arranged by size: 3074 951 837 682 552 425 276 144 117 32 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2146 2927 3597 3692 5141 5158 5311 Size: 1255 566 781 670 95 1449 17 153 Cuts at: 5311 5496 5941 325 Size: 185 445 1498 Fragments arranged by size: 1498 1449 1255 781 670 566 445 185 153 95 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2958 3915 5360 Size: 274 80 33 51 1443 981 957 1445 Cuts at: 5360 7072 96 Size: 1712 138 Fragments arranged by size: 1712 1445 1443 981 957 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7074 7106 7074 Size: 32 7082 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7114 XbaI T'CTAG_A Cuts at: 4450 4450 Size: 7114 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2917 2917 Size: 7114 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7114 XmaI C'CCGG_G Cuts at: 1870 3712 4439 1870 Size: 1842 727 4545 Fragments arranged by size: 4545 1842 727 XmnI GAAnn'nnTTC Cuts at: 4200 5243 4200 Size: 1043 6071 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4922 276 Size: 53 83 186 936 3388 2468 Fragments arranged by size: 3388 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacII SanDI SapI SfoI SrfI SwaI