Restriction map for pFN21AB3104
 (Linear) (Six-base) MAPSORT of: KIBB3104.seq  Check: 1238  from: 1  to: 2409



KIBB3104.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2023    2409 
   Size:       2023     386
 
AccI GT'mk_AC

Cuts at:      0     457    2409 
   Size:        457    1952
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     207     523    1413    1425    1666    1921    2337    2409 
   Size:        207     316     890      12     241     255     416      72

  Fragments arranged by size: 

                 890     416     316     255     241     207      72      12
            
 
AfeI AGC'GCT

Cuts at:      0      24    1434    2409 
   Size:         24    1410     975

  Fragments arranged by size: 

                1410     975      24
 
AflIII A'CryG_T

Cuts at:      0     612     882    1025    1554    2409 
   Size:        612     270     143     529     855

  Fragments arranged by size: 

                 855     612     529     270     143
 
AgeI A'CCGG_T

Cuts at:      0    2321    2409 
   Size:       2321      88
 
AleI CACnn'nnGTG

Cuts at:      0     726     967    2409 
   Size:        726     241    1442

  Fragments arranged by size: 

                1442     726     241
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2147    2179    2409 
   Size:       2147      32     230

  Fragments arranged by size: 

                2147     230      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      60     683    1644    2166    2316    2409 
   Size:         60     623     961     522     150      93

  Fragments arranged by size: 

                 961     623     522     150      93      60
 
ApaI G_GGCC'C

Cuts at:      0     559    2409 
   Size:        559    1850
 
ApaLI G'TGCA_C

Cuts at:      0     608    1504    2409 
   Size:        608     896     905

  Fragments arranged by size: 

                 905     896     608
 
ApoI r'AATT_y

Cuts at:      0     239     277     482    1189    2195    2409 
   Size:        239      38     205     707    1006     214

  Fragments arranged by size: 

                1006     707     239     214     205      38
 
AvaI C'yCGr_G

Cuts at:      0     250    1707    2409 
   Size:        250    1457     702

  Fragments arranged by size: 

                1457     702     250
 
BamHI G'GATC_C

Cuts at:      0     495    2357    2409 
   Size:        495    1862      52

  Fragments arranged by size: 

                1862     495      52
 
BanI G'GyrC_C

Cuts at:      0    1702    2409 
   Size:       1702     707
 
BanII G_rGCy'C

Cuts at:      0     559     797    2409 
   Size:        559     238    1612

  Fragments arranged by size: 

                1612     559     238
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     236     715    2409 
   Size:        236     479    1694

  Fragments arranged by size: 

                1694     479     236
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     732     766     975    1009    2409 
   Size:        732      34     209      34    1400

  Fragments arranged by size: 

                1400     732     209      34      34
 
BclI T'GATC_A

Cuts at:      0    2168    2409 
   Size:       2168     241
 
BglI GCCn_nnn'nGGC

Cuts at:      0     563    2409 
   Size:        563    1846
 
BglII A'GATC_T

Cuts at:      0     216    1310    2409 
   Size:        216    1094    1099

  Fragments arranged by size: 

                1099    1094     216
 
Bme1580I G_kGCm'C

Cuts at:      0     559     612    1508    2409 
   Size:        559      53     896     901

  Fragments arranged by size: 

                 901     896     559      53
 
BmgBI CAC'GTC

Cuts at:      0    1272    2409 
   Size:       1272    1137
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1275    2409 
   Size:       1275    1134
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      92    2409 
   Size:         92    2317
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383     937    1882    2283    2409 
   Size:        383     554     945     401     126

  Fragments arranged by size: 

                 945     554     401     383     126
 
Bpu10I CC'TnA_GC

Cuts at:      0     475    1321    1963    2409 
   Size:        475     846     642     446

  Fragments arranged by size: 

                 846     642     475     446
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1453    2409 
   Size:       1453     956
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     645     737    2409 
   Size:        645      92    1672

  Fragments arranged by size: 

                1672     645      92
 
BsaHI Gr'CG_yC

Cuts at:      0    1049    2409 
   Size:       1049    1360
 
BsaWI w'CCGG_w

Cuts at:      0     735    2321    2409 
   Size:        735    1586      88

  Fragments arranged by size: 

                1586     735      88
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     796     826    2409 
   Size:        796      30    1583

  Fragments arranged by size: 

                1583     796      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     712    1879    2409 
   Size:        712    1167     530

  Fragments arranged by size: 

                1167     712     530
 
BseYI C'CCAG_C

Cuts at:      0     868    1217    1745    1851    1980    2409 
   Size:        868     349     528     106     129     429

  Fragments arranged by size: 

                 868     528     429     349     129     106
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61    1050    1404    1816    2409 
   Size:         61     989     354     412     593

  Fragments arranged by size: 

                 989     593     412     354      61
 
BsiEI CG_ry'CG

Cuts at:      0    1800    2409 
   Size:       1800     609
 
BsiHKAI G_wGCw'C

Cuts at:      0     612     797    1327    1508    1969    2409 
   Size:        612     185     530     181     461     440

  Fragments arranged by size: 

                 612     530     461     440     185     181
 
BsmI GAATG_Cn'

Cuts at:      0    2353    2409 
   Size:       2353      56
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1338    1744    2409 
   Size:       1338     406     665

  Fragments arranged by size: 

                1338     665     406
 
Bsp1286I G_dGCh'C

Cuts at:      0     559     612     797    1327    1508    1969    2409 
   Size:        559      53     185     530     181     461     440

  Fragments arranged by size: 

                 559     530     461     440     185     181      53
 
BspEI T'CCGG_A

Cuts at:      0     735    2409 
   Size:        735    1674
 
BspHI T'CATG_A

Cuts at:      0     243    2409 
   Size:        243    2166
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1343    1637    2023    2409 
   Size:       1343     294     386     386

  Fragments arranged by size: 

                1343     386     386     294
 
BsrDI GCAATG_nn'

Cuts at:      0     534    2409 
   Size:        534    1875
 
BsrFI r'CCGG_y

Cuts at:      0     808    2321    2409 
   Size:        808    1513      88

  Fragments arranged by size: 

                1513     808      88
 
BssSI C'ACGA_G

Cuts at:      0     744    1078    2409 
   Size:        744     334    1331

  Fragments arranged by size: 

                1331     744     334
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     636    1371    2409 
   Size:        636     735    1038

  Fragments arranged by size: 

                1038     735     636
 
BstYI r'GATC_y

Cuts at:      0     216     495    1280    1310    2357    2409 
   Size:        216     279     785      30    1047      52

  Fragments arranged by size: 

                1047     785     279     216      52      30
 
BstZ17I GTA'TAC

Cuts at:      0     458    2409 
   Size:        458    1951
 
BtgI C'CryG_G

Cuts at:      0     940     965    1055    1770    1800    2091    2409 
   Size:        940      25      90     715      30     291     318

  Fragments arranged by size: 

                 940     715     318     291      90      30      25
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     702    2409 
   Size:        702    1707
 
BtsI GCAGTG_nn'

Cuts at:      0     109    1396    2180    2409 
   Size:        109    1287     784     229

  Fragments arranged by size: 

                1287     784     229     109
 
DraIII CAC_nnn'GTG

Cuts at:      0    1839    2409 
   Size:       1839     570
 
EaeI y'GGCC_r

Cuts at:      0    1140    1797    1931    2409 
   Size:       1140     657     134     478

  Fragments arranged by size: 

                1140     657     478     134
 
EagI C'GGCC_G

Cuts at:      0    1797    2409 
   Size:       1797     612
 
EarI CTCTTCn'nnn_

Cuts at:      0     226     689    2409 
   Size:        226     463    1720

  Fragments arranged by size: 

                1720     463     226
 
EcoICRI GAG'CTC

Cuts at:      0     795    2409 
   Size:        795    1614
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     207     383     523     937    1413    1425    1666    1882 
   Size:        207     176     140     414     476      12     241     216

Cuts at:   1882    1921    2283    2337    2409 
   Size:         39     362      54      72

  Fragments arranged by size: 

                 476     414     362     241     216     207     176     140
                  72      54      39      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0     555     556    1332    1480    2409 
   Size:        555       1     776     148     929

  Fragments arranged by size: 

                 929     776     555     148       1
 
EcoRI G'AATT_C

Cuts at:      0     277    2409 
   Size:        277    2132
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1170    1332    2409 
   Size:       1170     162    1077

  Fragments arranged by size: 

                1170    1077     162
 
HaeII r_GCGC'y

Cuts at:      0      26    1436    2409 
   Size:         26    1410     973

  Fragments arranged by size: 

                1410     973      26
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     455     487    1337    1369    2160    2192    2409 
   Size:        455      32     850      32     791      32     217

  Fragments arranged by size: 

                 850     791     455     217      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     535    2409 
   Size:        535    1874
 
MfeI C'AATT_G

Cuts at:      0     104    2409 
   Size:        104    2305
 
MslI CAynn'nnrTG

Cuts at:      0     634     726     964     967    1082    1204    2409 
   Size:        634      92     238       3     115     122    1205

  Fragments arranged by size: 

                1205     634     238     122     115      92       3
 
MspA1I CmG'CkG

Cuts at:      0     172     573    1808    2409 
   Size:        172     401    1235     601

  Fragments arranged by size: 

                1235     601     401     172
 
NcoI C'CATG_G

Cuts at:      0     965    2091    2409 
   Size:        965    1126     318

  Fragments arranged by size: 

                1126     965     318
 
NspI r_CATG'y

Cuts at:      0     616    1558    2409 
   Size:        616     942     851

  Fragments arranged by size: 

                 942     851     616
 
PasI CC'CwG_GG

Cuts at:      0    1173    2409 
   Size:       1173    1236
 
PciI A'CATG_T

Cuts at:      0     612    1554    2409 
   Size:        612     942     855

  Fragments arranged by size: 

                 942     855     612
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     915    1452    2409 
   Size:        915     537     957

  Fragments arranged by size: 

                 957     915     537
 
PfoI T'CCnGG_A

Cuts at:      0    2360    2409 
   Size:       2360      49
 
PpuMI rG'GwC_Cy

Cuts at:      0    1332    1480    2409 
   Size:       1332     148     929

  Fragments arranged by size: 

                1332     929     148
 
PspOMI G'GGCC_C

Cuts at:      0     555    2409 
   Size:        555    1854
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1408    1440    2409 
   Size:       1408      32     969

  Fragments arranged by size: 

                1408     969      32
 
PvuII CAG'CTG

Cuts at:      0     573    2409 
   Size:        573    1836
 
SacI G_AGCT'C

Cuts at:      0     797    2409 
   Size:        797    1612
 
ScaI AGT'ACT

Cuts at:      0     955    2409 
   Size:        955    1454
 
SexAI A'CCwGG_T

Cuts at:      0    1406    2409 
   Size:       1406    1003
 
SfcI C'TryA_G

Cuts at:      0     209     285    1356    2409 
   Size:        209      76    1071    1053

  Fragments arranged by size: 

                1071    1053     209      76
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     563    2409 
   Size:        563    1846
 
SmaI CCC'GGG

Cuts at:      0    1709    2409 
   Size:       1709     700
 
SmlI C'TyrA_G

Cuts at:      0    1468    2409 
   Size:       1468     941
 
StuI AGG'CCT

Cuts at:      0    1096    2409 
   Size:       1096    1313
 
StyI C'CwwG_G

Cuts at:      0      11     848     965     989    1133    1815    2091    2123 
   Size:         11     837     117      24     144     682     276      32

Cuts at:   2123    2409 
   Size:        286

  Fragments arranged by size: 

                 837     682     286     276     144     117      32      24
                  11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     141     922    1592    1687    2409 
   Size:        141     781     670      95     722

  Fragments arranged by size: 

                 781     722     670     141      95
 
TatI w'GTAC_w

Cuts at:      0     953    1910    2409 
   Size:        953     957     499

  Fragments arranged by size: 

                 957     953     499
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     912    2409 
   Size:        912    1497
 
XmaI C'CCGG_G

Cuts at:      0    1707    2409 
   Size:       1707     702
 
XmnI GAAnn'nnTTC

Cuts at:      0    2195    2409 
   Size:       2195     214
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AgeI     AleI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII
     BbsI     BcgI     BclI     BglI    BglII Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrDI    BsrFI    BssSI    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     BtsI   DraIII     EaeI     EagI     EarI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI    HaeII    Hin4I  HindIII
     MfeI     MslI   MspA1I     NcoI     NspI     PasI     PciI    PflMI
     PfoI    PpuMI   PspOMI     PsrI    PvuII     SacI     ScaI    SexAI
     SfcI     SfiI     SmaI     SmlI     StuI     StyI    TaqII     TatI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AhdI     AlfI     AscI     AseI
    AsiSI    AvrII     BaeI     BbeI    BbvCI    BciVI    BfrBI     BlpI
     BmtI    BsaAI    BsaBI    BsiWI    BsrBI    BsrGI   BssHII   BstAPI
    BstBI   BstEII   Bsu36I     ClaI    CspCI     DraI     DrdI     EciI
    EcoNI    EcoRV     FseI     FspI    FspAI   HincII     HpaI     KasI
     KpnI     MluI     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PstI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XhoI
     ZraI
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