Restriction map for
pFN21AB3100
(Linear) (Six-base) MAPSORT of: KIBB3100.seq Check: 7501 from: 1 to: 2568
KIBB3100.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2352 2568
Size: 2352 216
AccI GT'mk_AC
Cuts at: 0 820 2568
Size: 820 1748
Acc65I G'GTAC_C
Cuts at: 0 1360 2568
Size: 1360 1208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 488 617 2568
Size: 37 451 129 1951
Fragments arranged by size:
1951 451 129 37
AfeI AGC'GCT
Cuts at: 0 1822 1882 2568
Size: 1822 60 686
Fragments arranged by size:
1822 686 60
AlwNI CAG_nnn'CTG
Cuts at: 0 829 2568
Size: 829 1739
AvaI C'yCGr_G
Cuts at: 0 65 169 241 276 674 1182 2568
Size: 65 104 72 35 398 508 1386
Fragments arranged by size:
1386 508 398 104 72 65 35
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 326 359 614 647 2568
Size: 326 33 255 33 1921
Fragments arranged by size:
1921 326 255 33 33
BanI G'GyrC_C
Cuts at: 0 1231 1360 2568
Size: 1231 129 1208
Fragments arranged by size:
1231 1208 129
BanII G_rGCy'C
Cuts at: 0 143 208 266 738 1166 2073 2568
Size: 143 65 58 472 428 907 495
Fragments arranged by size:
907 495 472 428 143 65 58
BbsI GAAGACnn'nnnn_
Cuts at: 0 430 2568
Size: 430 2138
BbvCI CC'TCA_GC
Cuts at: 0 584 2426 2568
Size: 584 1842 142
Fragments arranged by size:
1842 584 142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 391 425 2568
Size: 391 34 2143
Fragments arranged by size:
2143 391 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1234 2568
Size: 1234 1334
BglI GCCn_nnn'nGGC
Cuts at: 0 243 492 2568
Size: 243 249 2076
Fragments arranged by size:
2076 249 243
BlpI GC'TnA_GC
Cuts at: 0 89 2568
Size: 89 2479
Bme1580I G_kGCm'C
Cuts at: 0 176 1234 2568
Size: 176 1058 1334
Fragments arranged by size:
1334 1058 176
BmgBI CAC'GTC
Cuts at: 0 1020 2568
Size: 1020 1548
BmrI ACTGGGnnnn_n'
Cuts at: 0 929 1449 2568
Size: 929 520 1119
Fragments arranged by size:
1119 929 520
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 222 816 915 2568
Size: 222 594 99 1653
Fragments arranged by size:
1653 594 222 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 1925 2568
Size: 235 1690 643
Fragments arranged by size:
1690 643 235
Bpu10I CC'TnA_GC
Cuts at: 0 584 2426 2568
Size: 584 1842 142
Fragments arranged by size:
1842 584 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 466 2196 2568
Size: 58 408 1730 372
Fragments arranged by size:
1730 408 372 58
BsaI GGTCTCn'nnnn_
Cuts at: 0 187 372 1548 2568
Size: 187 185 1176 1020
Fragments arranged by size:
1176 1020 187 185
BsaAI yAC'GTr
Cuts at: 0 840 2568
Size: 840 1728
BsaBI GATnn'nnATC
Cuts at: 0 784 2568
Size: 784 1784
BsaHI Gr'CG_yC
Cuts at: 0 146 2568
Size: 146 2422
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2026 2056 2568
Size: 2026 30 512
Fragments arranged by size:
2026 512 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 133 199 390 572 1967 1982 2568
Size: 52 81 66 191 182 1395 15 586
Fragments arranged by size:
1395 586 191 182 81 66 52 15
BseYI C'CCAG_C
Cuts at: 0 878 1427 1801 2012 2075 2123 2143 2431
Size: 878 549 374 211 63 48 20 288
Cuts at: 2431 2459 2568
Size: 28 109
Fragments arranged by size:
878 549 374 288 211 109 63 48
28 20
BsiHKAI G_wGCw'C
Cuts at: 0 143 447 557 2568
Size: 143 304 110 2011
Fragments arranged by size:
2011 304 143 110
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1536 2568
Size: 1536 1032
Bsp1286I G_dGCh'C
Cuts at: 0 143 176 208 266 447 557 738 1166
Size: 143 33 32 58 181 110 181 428
Cuts at: 1166 1234 2073 2568
Size: 68 839 495
Fragments arranged by size:
839 495 428 181 181 143 110 68
58 33 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2352 2568
Size: 2352 216
BsrBI CCG'CTC
Cuts at: 0 93 135 578 699 2568
Size: 93 42 443 121 1869
Fragments arranged by size:
1869 443 121 93 42
BsrDI GCAATG_nn'
Cuts at: 0 1058 2568
Size: 1058 1510
BsrFI r'CCGG_y
Cuts at: 0 941 1403 2568
Size: 941 462 1165
Fragments arranged by size:
1165 941 462
BtgI C'CryG_G
Cuts at: 0 51 299 1857 1914 2568
Size: 51 248 1558 57 654
Fragments arranged by size:
1558 654 248 57 51
BtsI GCAGTG_nn'
Cuts at: 0 1139 2470 2568
Size: 1139 1331 98
Fragments arranged by size:
1331 1139 98
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1818 1853 2568
Size: 1818 35 715
Fragments arranged by size:
1818 715 35
DraIII CAC_nnn'GTG
Cuts at: 0 1457 2551 2568
Size: 1457 1094 17
Fragments arranged by size:
1457 1094 17
EaeI y'GGCC_r
Cuts at: 0 1422 2497 2568
Size: 1422 1075 71
Fragments arranged by size:
1422 1075 71
EarI CTCTTCn'nnn_
Cuts at: 0 593 884 2568
Size: 593 291 1684
Fragments arranged by size:
1684 593 291
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2093 2568
Size: 2093 475
EcoICRI GAG'CTC
Cuts at: 0 141 2568
Size: 141 2427
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 235 488 617 1925 2568
Size: 37 198 253 129 1308 643
Fragments arranged by size:
1308 643 253 198 129 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1723 2568
Size: 1723 845
EcoO109I rG'GnC_Cy
Cuts at: 0 1098 1608 1897 2568
Size: 1098 510 289 671
Fragments arranged by size:
1098 671 510 289
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1101 2568
Size: 1101 1467
FspI TGC'GCA
Cuts at: 0 1131 2568
Size: 1131 1437
HaeII r_GCGC'y
Cuts at: 0 51 1824 1884 2219 2568
Size: 51 1773 60 335 349
Fragments arranged by size:
1773 349 335 60 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2409 2441 2568
Size: 2409 32 127
Fragments arranged by size:
2409 127 32
HindIII A'AGCT_T
Cuts at: 0 470 2568
Size: 470 2098
KpnI G_GTAC'C
Cuts at: 0 1364 2568
Size: 1364 1204
MfeI C'AATT_G
Cuts at: 0 704 2568
Size: 704 1864
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 1218 1355 2069 2568
Size: 89 1129 137 714 499
Fragments arranged by size:
1129 714 499 137 89
MslI CAynn'nnrTG
Cuts at: 0 723 2568
Size: 723 1845
MspA1I CmG'CkG
Cuts at: 0 53 1410 1427 1749 1859 1946 1985 2012
Size: 53 1357 17 322 110 87 39 27
Cuts at: 2012 2568
Size: 556
Fragments arranged by size:
1357 556 322 110 87 53 39 27
17
NaeI GCC'GGC
Cuts at: 0 1405 2568
Size: 1405 1163
NcoI C'CATG_G
Cuts at: 0 299 2568
Size: 299 2269
NgoMIV G'CCGG_C
Cuts at: 0 1403 2568
Size: 1403 1165
NruI TCG'CGA
Cuts at: 0 35 2568
Size: 35 2533
NspI r_CATG'y
Cuts at: 0 1324 2568
Size: 1324 1244
PasI CC'CwG_GG
Cuts at: 0 2170 2568
Size: 2170 398
PflMI CCAn_nnn'nTGG
Cuts at: 0 1708 2568
Size: 1708 860
PfoI T'CCnGG_A
Cuts at: 0 1718 2536 2568
Size: 1718 818 32
Fragments arranged by size:
1718 818 32
PmlI CAC'GTG
Cuts at: 0 840 2568
Size: 840 1728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1717 1749 2568
Size: 1717 32 819
Fragments arranged by size:
1717 819 32
PpuMI rG'GwC_Cy
Cuts at: 0 1608 1897 2568
Size: 1608 289 671
Fragments arranged by size:
1608 671 289
PspXI vC'TCGA_Gb
Cuts at: 0 65 2568
Size: 65 2503
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 313 345 2506 2538 2568
Size: 313 32 2161 32 30
Fragments arranged by size:
2161 313 32 32 30
PstI C_TGCA'G
Cuts at: 0 47 101 251 874 1748 2568
Size: 47 54 150 623 874 820
Fragments arranged by size:
874 820 623 150 54 47
SacI G_AGCT'C
Cuts at: 0 143 2568
Size: 143 2425
SacII CC_GC'GG
Cuts at: 0 54 1860 2568
Size: 54 1806 708
Fragments arranged by size:
1806 708 54
SapI GCTCTTCn'nnn_
Cuts at: 0 884 2568
Size: 884 1684
ScaI AGT'ACT
Cuts at: 0 2476 2568
Size: 2476 92
SfcI C'TryA_G
Cuts at: 0 43 97 247 821 870 1744 1998 2148
Size: 43 54 150 574 49 874 254 150
Cuts at: 2148 2247 2568
Size: 99 321
Fragments arranged by size:
874 574 321 254 150 150 99 54
49 43
SmaI CCC'GGG
Cuts at: 0 171 243 1184 2568
Size: 171 72 941 1384
Fragments arranged by size:
1384 941 171 72
SmlI C'TyrA_G
Cuts at: 0 65 73 481 2211 2568
Size: 65 8 408 1730 357
Fragments arranged by size:
1730 408 357 65 8
SrfI GCCC'GGGC
Cuts at: 0 243 2568
Size: 243 2325
SspI AAT'ATT
Cuts at: 0 309 2568
Size: 309 2259
StuI AGG'CCT
Cuts at: 0 71 406 519 2568
Size: 71 335 113 2049
Fragments arranged by size:
2049 335 113 71
StyI C'CwwG_G
Cuts at: 0 299 1085 2568
Size: 299 786 1483
Fragments arranged by size:
1483 786 299
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 211 462 892 1468 1634 2157 2568
Size: 211 251 430 576 166 523 411
Fragments arranged by size:
576 523 430 411 251 211 166
TatI w'GTAC_w
Cuts at: 0 1346 1778 2474 2560 2568
Size: 1346 432 696 86 8
Fragments arranged by size:
1346 696 432 86 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1004 1036 2568
Size: 1004 32 1532
Fragments arranged by size:
1532 1004 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1371 2568
Size: 1371 1197
XhoI C'TCGA_G
Cuts at: 0 65 2568
Size: 65 2503
XmaI C'CCGG_G
Cuts at: 0 169 241 1182 2568
Size: 169 72 941 1386
Fragments arranged by size:
1386 941 169 72
XmnI GAAnn'nnTTC
Cuts at: 0 325 2568
Size: 325 2243
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AlwNI AvaI BaeI
BanI BanII BbsI BbvCI BcgI BciVI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BtgI BtsI CspCI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HindIII KpnI MfeI MmeI
MslI MspA1I NaeI NcoI NgoMIV NruI NspI PasI
PflMI PfoI PmlI PpiI PpuMI PspXI PsrI PstI
SacI SacII SapI ScaI SfcI SmaI SmlI SrfI
SspI StuI StyI TaqII TatI TstI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AleI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BamHI BbeI BclI BfrBI BglII BmtI BsaWI BsgI
BsiEI BsiWI BsmI BspEI BspHI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
ClaI DraI DrdI EagI EcoRI EcoRV FseI FspAI
HincII HpaI KasI MluI MscI NarI NdeI NheI
NotI NsiI PacI PciI PmeI PshAI PsiI PspOMI
PvuI PvuII RsrII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SwaI Tth111I
XbaI ZraI