Restriction map for
pFN21AB3093
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# NO CUT ENZYMES ( 105 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I AlwI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AvrII
BamHI BbrPI BbsI BbvCI BfrI
BlnI BpuAI BsaI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstBI BstZ17I Cfr10I
ClaI CpoI DraI DraIII Eco47III
EcoRI EcoRV EcoT22I FseI HindIII
HpaI KpnI KspI MfeI MluI
MroI MseI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
Tsp509I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 1210 GACGTC GACGT^C
AhdI 987 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 987 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1127 TGCGCA TGC^GCA
BcgI 1914 CGAGGGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 74 GTATCC GTATCC(6/5)
BfaI 1388 CTAG C^TAG
BglII 1736 AGATCT A^GATCT
BlpI 702 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 702 GCTCAGC GC^T[ACGT]AGC
BsaAI 1621 CACGTA [CT]AC^GT[GA]
BsaBI 2058 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1850 CGGCCG CG[GA][CT]^CG
BsmBI 1429 CGTCTC CGTCTC(1/5)
BssSI 943 CTCGTG CACGAG(-5/-1)
BstEII 474 GGTGACC G^GT[ACGT]ACC
BstPI 474 GGTGACC G^GT[ACGT]ACC
BstYI 1736 AGATCT [GA]^GATC[CT]
CelII 702 GCTCAGC GC^T[ACGT]AGC
DrdI 1210 GACGTCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1850 CGGCCG C^GGCCG
Eam1105I 987 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2014 CTCTTC CTCTTC(1/4)
EclXI 1850 CGGCCG C^GGCCG
Eco52I 1850 CGGCCG C^GGCCG
EcoNI 762 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 474 GGTGACC G^GT[ACGT]ACC
FspI 1127 TGCGCA TGC^GCA
HincII 1213 GTCAAC GT[CT]^[GA]AC
HindII 1213 GTCAAC GT[CT]^[GA]AC
Ksp632I 2014 CTCTTC CTCTTC(1/4)
MaeI 1388 CTAG C^TAG
MamI 2058 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1736 AGATCT [GA]^GATC[CT]
SfiI 1404 GGCCGAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1712 CCCGGG CCC^GGG
SphI 698 GCATGC GCATG^C
StuI 140 AGGCCT AGG^CCT
XcmI 1721 CCATGAACCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1736 AGATCT [GA]^GATC[CT]
XmaCI 1712 CCCGGG C^CCGGG
XmaI 1712 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AocI 1399 CCTGAGG CC^T[ACGT]AGG
2196 CCTCAGG
ApaI 530 GGGCCC GGGCC^C
931 GGGCCC
BclI 911 TGATCA T^GATCA
1511 TGATCA
BsaWI 1690 ACCGGA [AT]^CCGG[AT]
1979 ACCGGA
BseRI 130 GAGGAG GAGGAG(10/8)
1669 GAGGAG
BsmAI 2165 GAGAC GTCTC(1/5)
1430 GTCTC
BstAPI 750 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
948 GCAGATCATGC
Bsu36I 1399 CCTGAGG CC^T[ACGT]AGG
2196 CCTCAGG
DsaI 1232 CCACGG C^C[GA][CT]GG
1994 CCGTGG
Eco81I 1399 CCTGAGG CC^T[ACGT]AGG
2196 CCTCAGG
EcoT14I 204 CCAAGG C^C[AT][AT]GG
479 CCAAGG
FbaI 911 TGATCA T^GATCA
1511 TGATCA
PleI 67 GACTC GAGTC(4/5)
718 GAGTC
PpuMI 461 AGGACCC [GA]G^G[AT]CC[CT]
1715 GGGACCC
SacI 436 GAGCTC GAGCT^C
1999 GAGCTC
StyI 204 CCAAGG C^C[AT][AT]GG
479 CCAAGG
TaqI 508 TCGA T^CGA
2177 TCGA
TthHB8I 508 TCGA T^CGA
2177 TCGA
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvI BcnI
BglI BmyI BpmI BsaHI BsaJI
BsgI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PflMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI TfiI TseI
Tsp45I TspRI Van91I