Restriction map for
pFN21AB3090
(Circular) (Six-base) MAPSORT of: pFN21AB3090.seq Check: 206 from: 1 to: 6559
pFN21AB3090 6559 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4369 278
Size: 53 83 186 936 2833 2468
Fragments arranged by size:
2833 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2156 3902 1937
Size: 219 1746 4594
Fragments arranged by size:
4594 1746 219
Acc65I G'GTAC_C
Cuts at: 3880 3880
Size: 6559
AclI AA'CG_TT
Cuts at: 1563 4686 5059 1563
Size: 3123 373 3063
Fragments arranged by size:
3123 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2574 3075 3141 3336 3375 4622 6006 6274
Size: 942 501 66 195 39 1247 1384 268
Cuts at: 6274 1632
Size: 1917
Fragments arranged by size:
1917 1384 1247 942 501 268 195 66
39
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6362
AflIII A'CryG_T
Cuts at: 1203 3422 5458 6435 1203
Size: 2219 2036 977 1327
Fragments arranged by size:
2219 2036 1327 977
AgeI A'CCGG_T
Cuts at: 5390 5390
Size: 6559
AhdI GACnn_n'nnGTC
Cuts at: 5288 5288
Size: 6559
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6559
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2031 2826 1184
Size: 847 795 4917
Fragments arranged by size:
4917 847 795
AlwNI CAG_nnn'CTG
Cuts at: 2350 2691 4302 5874 2350
Size: 341 1611 1572 3035
Fragments arranged by size:
3035 1611 1572 341
ApaLI G'TGCA_C
Cuts at: 4616 5772 4616
Size: 1156 5403
ApoI r'AATT_y
Cuts at: 1493 2758 3868 4094 4246 6355 1493
Size: 1265 1110 226 152 2109 1697
Fragments arranged by size:
2109 1697 1265 1110 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3383 3468 5113 160
Size: 872 2351 85 1645 1606
Fragments arranged by size:
2351 1645 1606 872 85
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6559
AvaI C'yCGr_G
Cuts at: 1870 1943 3884 1870
Size: 73 1941 4545
Fragments arranged by size:
4545 1941 73
BamHI G'GATC_C
Cuts at: 2369 3889 4256 2369
Size: 1520 367 4672
Fragments arranged by size:
4672 1520 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2182 3880 5335 618
Size: 325 217 627 395 1698 1455 1842
Fragments arranged by size:
1842 1698 1455 627 395 325 217
BanII G_rGCy'C
Cuts at: 1391 3878 1391
Size: 2487 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2511 928
Size: 971 612 4976
Fragments arranged by size:
4976 971 612
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4750 4784 1903
Size: 34 50 34 2729 34 3678
Fragments arranged by size:
3678 2729 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4451 5661 4451
Size: 1210 5349
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6559
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5170 136
Size: 107 122 71 631 605 3498 1525
Fragments arranged by size:
3498 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6554 6554
Size: 6559
BlpI GC'TnA_GC
Cuts at: 3976 3976
Size: 6559
Bme1580I G_kGCm'C
Cuts at: 1790 3793 4620 5776 1790
Size: 2003 827 1156 2573
Fragments arranged by size:
2573 2003 1156 827
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6559
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2701 4290 5248
Size: 416 53 83 399 24 1284 1589 958
Cuts at: 5248 442
Size: 1753
Fragments arranged by size:
1753 1589 1284 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6559
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2223 2223
Size: 6559
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2449 2667 2979 3123 3165 3285 5219 1239
Size: 1210 218 312 144 42 120 1934 2579
Fragments arranged by size:
2579 1934 1210 312 218 144 120 42
Bpu10I CC'TnA_GC
Cuts at: 2865 2865
Size: 6559
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2962 4684 5549 5847 2962
Size: 1722 865 298 3674
Fragments arranged by size:
3674 1722 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3216 5222 882
Size: 617 1717 2006 2219
Fragments arranged by size:
2219 2006 1717 617
BsaAI yAC'GTr
Cuts at: 493 1204 4350 493
Size: 711 3146 2702
Fragments arranged by size:
3146 2702 711
BsaBI GATnn'nnATC
Cuts at: 6553 6553
Size: 6559
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4366 4748 5383 275
Size: 53 83 186 936 2833 382 635 1451
Fragments arranged by size:
2833 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2724 4992 5390 5664 5811 6222 2724
Size: 2268 398 274 147 411 3061
Fragments arranged by size:
3061 2268 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2640 2670 1178
Size: 30 165 30 1237 30 5067
Fragments arranged by size:
5067 1237 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3350 3706 1186
Size: 2164 356 4039
Fragments arranged by size:
4039 2164 356
BseYI C'CCAG_C
Cuts at: 5762 6258 5762
Size: 496 6063
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5986
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4009 4770 5798 1540
Size: 275 186 2008 761 1028 2301
Fragments arranged by size:
2301 2008 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4620 4705 5776 4620
Size: 85 1071 5403
Fragments arranged by size:
5403 1071 85
BsmI GAATG_Cn'
Cuts at: 4070 4163 4070
Size: 93 6466
BsmBI CGTCTCn'nnnn_
Cuts at: 2394 2394
Size: 6559
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3793 3878 4620 4705 5776 1391
Size: 399 2003 85 742 85 1071 2174
Fragments arranged by size:
2174 2003 1071 742 399 85 85
BspEI T'CCGG_A
Cuts at: 6222 6222
Size: 6559
BspHI T'CATG_A
Cuts at: 3171 4448 6178 3171
Size: 1277 1730 3552
Fragments arranged by size:
3552 1730 1277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3914 844
Size: 3070 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4446 1394
Size: 429 2623 3507
Fragments arranged by size:
3507 2623 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2550 2911 5054 5228 65
Size: 1183 1302 361 2143 174 1396
Fragments arranged by size:
2143 1396 1302 1183 361 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5203 5390 1700
Size: 110 3393 187 2869
Fragments arranged by size:
3393 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6559
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6559
BssSI C'ACGA_G
Cuts at: 2208 4619 5631 2208
Size: 2411 1012 3136
Fragments arranged by size:
3136 2411 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6559
BstBI TT'CG_AA
Cuts at: 5363 5363
Size: 6559
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6559
BstYI r'GATC_y
Cuts at: 1972 2369 3889 4256 4641 4658 6110 6196 6208
Size: 397 1520 367 385 17 1452 86 12
Cuts at: 6208 6554 1972
Size: 346 1977
Fragments arranged by size:
1977 1520 1452 397 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2969 513
Size: 552 1904 4103
Fragments arranged by size:
4103 1904 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5820
Fragments arranged by size:
5820 683 56
BtsI GCAGTG_nn'
Cuts at: 4075 4869 4889 4075
Size: 794 20 5745
Fragments arranged by size:
5745 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6559
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6524
DraI TTT'AAA
Cuts at: 3453 3863 3453
Size: 410 6149
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5566 809
Size: 507 4250 1802
Fragments arranged by size:
4250 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2585 2840 3564
Size: 54 1425 50 275 18 755 255 724
Cuts at: 3564 4006 4895 6524 8
Size: 442 889 1629 43
Fragments arranged by size:
1629 1425 889 755 724 442 275 255
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4006 1537
Size: 275 2194 4090
Fragments arranged by size:
4090 2194 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2518 3327 4489 1353
Size: 1165 809 1162 3423
Fragments arranged by size:
3423 1165 1162 809
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5146 5520 5666 387
Size: 1064 138 3557 374 146 1280
Fragments arranged by size:
3557 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2449 2574 2667 2979 3075 3123 3141
Size: 393 817 125 93 312 96 48 18
Cuts at: 3141 3165 3285 3336 3375 4622 5219 6006 6274
Size: 24 120 51 39 1247 597 787 268
Cuts at: 6274 1239
Size: 1524
Fragments arranged by size:
1524 1247 817 787 597 393 312 268
125 120 96 93 51 48 39 24
18
EcoO109I rG'GnC_Cy
Cuts at: 2039 2642 2039
Size: 603 5956
EcoRI G'AATT_C
Cuts at: 3868 4246 3868
Size: 378 6181
EcoRV GAT'ATC
Cuts at: 3059 3059
Size: 6559
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2146 2146
Size: 6559
FspI TGC'GCA
Cuts at: 5065 5065
Size: 6559
HaeII r_GCGC'y
Cuts at: 1678 5706 1678
Size: 4028 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2699 2731 3281 3313 3362
Size: 32 135 32 1127 32 550 32 49
Cuts at: 3362 3394 5200 5232 5274 5306 1373
Size: 32 1806 32 42 32 2626
Fragments arranged by size:
2626 1806 1127 550 135 49 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3087 3903 4149 669
Size: 988 281 1149 816 246 3079
Fragments arranged by size:
3079 1149 988 816 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6559
HpaI GTT'AAC
Cuts at: 4149 4149
Size: 6559
KpnI G_GTAC'C
Cuts at: 3884 3884
Size: 6559
MfeI C'AATT_G
Cuts at: 3416 3686 4158 3416
Size: 270 472 5817
Fragments arranged by size:
5817 472 270
MluI A'CGCG_T
Cuts at: 6435 6435
Size: 6559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3674 5673 5857 1305
Size: 2369 1999 184 2007
Fragments arranged by size:
2369 2007 1999 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2587 2842 10
Size: 54 1425 343 755 255 3727
Fragments arranged by size:
3727 1425 755 343 255 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4517 4876 5035 518
Size: 648 3351 359 159 2042
Fragments arranged by size:
3351 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3079 3975 4652 5800 6045 1606
Size: 1473 896 677 1148 245 2120
Fragments arranged by size:
2120 1473 1148 896 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2969 513
Size: 552 1904 4103
Fragments arranged by size:
4103 1904 552
NdeI CA'TA_TG
Cuts at: 387 2580 387
Size: 2193 4366
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6559
NotI GC'GGCC_GC
Cuts at: 4006 4006
Size: 6559
NspI r_CATG'y
Cuts at: 1132 1229 2803 2813 2984 3426 3917 5462 1132
Size: 97 1574 10 171 442 491 1545 2229
Fragments arranged by size:
2229 1574 1545 491 442 171 97 10
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6559
PciI A'CATG_T
Cuts at: 3422 5458 3422
Size: 2036 4523
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6559
PfoI T'CCnGG_A
Cuts at: 3438 3438
Size: 6559
PmeI GTTT'AAAC
Cuts at: 3863 3863
Size: 6559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4628 4660 6141 6173 4628
Size: 32 1481 32 5014
Fragments arranged by size:
5014 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2039 2642 2039
Size: 603 5956
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6559
PsiI TTA'TAA
Cuts at: 4129 4129
Size: 6559
PstI C_TGCA'G
Cuts at: 830 3911 830
Size: 3081 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6559
PvuII CAG'CTG
Cuts at: 3079 3079
Size: 6559
SalI G'TCGA_C
Cuts at: 1936 3901 1936
Size: 1965 4594
SbfI CC_TGCA'GG
Cuts at: 3911 3911
Size: 6559
ScaI AGT'ACT
Cuts at: 2551 4807 6519 2551
Size: 2256 1712 2591
Fragments arranged by size:
2591 2256 1712
SexAI A'CCwGG_T
Cuts at: 3225 3225
Size: 6559
SfcI C'TryA_G
Cuts at: 826 1045 2146 2332 2533 3907 5042 5723 5914
Size: 219 1101 186 201 1374 1135 681 191
Cuts at: 5914 826
Size: 1471
Fragments arranged by size:
1471 1374 1135 1101 681 219 201 191
186
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6559
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6559
SmaI CCC'GGG
Cuts at: 1872 3886 1872
Size: 2014 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2941 4663 5564 5826 820
Size: 197 926 998 1722 901 262 1553
Fragments arranged by size:
1722 1553 998 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4350 493
Size: 3857 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6559
SphI G_CATG'C
Cuts at: 1132 2803 3917 1132
Size: 1671 1114 3774
Fragments arranged by size:
3774 1671 1114
SspI AAT'ATT
Cuts at: 5 52 4483 5
Size: 47 4431 2081
Fragments arranged by size:
4431 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6559
StyI C'CwwG_G
Cuts at: 513 1065 2430 2969 2996 3201 3998 513
Size: 552 1365 539 27 205 797 3074
Fragments arranged by size:
3074 1365 797 552 539 205 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4586 4603 4756 4941 5386 325
Size: 1255 3006 17 153 185 445 1498
Fragments arranged by size:
3006 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2549 2835 4805
Size: 274 80 33 51 1443 572 286 1970
Cuts at: 4805 6517 96
Size: 1712 138
Fragments arranged by size:
1970 1712 1443 572 286 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6519 6551 6519
Size: 32 6527
Tth111I GACn'n_nGTC
Cuts at: 1316 2243 1316
Size: 927 5632
XbaI T'CTAG_A
Cuts at: 2492 2764 3895 2492
Size: 272 1131 5156
Fragments arranged by size:
5156 1131 272
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6559
XmaI C'CCGG_G
Cuts at: 1870 3884 1870
Size: 2014 4545
XmnI GAAnn'nnTTC
Cuts at: 4688 4688
Size: 6559
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4367 276
Size: 53 83 186 936 2833 2468
Fragments arranged by size:
2833 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI