(Circular) (Six-base) MAPSORT of: pFN21AB3088.seq Check: 2821 from: 1 to: 5959 pFN21AB3088 5959 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2892 3302 1937 Size: 955 410 4594 Fragments arranged by size: 4594 955 410 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2743 4022 5406 5674 1632 Size: 1111 1279 1384 268 1917 Fragments arranged by size: 1917 1384 1279 1111 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 4858 5835 1203 Size: 3655 977 1327 Fragments arranged by size: 3655 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5959 AlwNI CAG_nnn'CTG Cuts at: 2175 3095 3702 5274 2175 Size: 920 607 1572 2860 Fragments arranged by size: 2860 1572 920 607 ApaLI G'TGCA_C Cuts at: 4016 5172 4016 Size: 1156 4803 ApoI r'AATT_y Cuts at: 1493 3268 3494 3646 5755 1493 Size: 1775 226 152 2109 1697 Fragments arranged by size: 2109 1775 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 3084 3284 1870 Size: 73 1141 200 4545 Fragments arranged by size: 4545 1141 200 73 BamHI G'GATC_C Cuts at: 2479 3289 3656 2479 Size: 810 367 4782 Fragments arranged by size: 4782 810 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2070 2144 2189 2207 2284 Size: 325 217 627 283 74 45 18 77 Cuts at: 2284 2882 3280 4735 618 Size: 598 398 1455 1842 Fragments arranged by size: 1842 1455 627 598 398 325 283 217 77 74 45 18 BanII G_rGCy'C Cuts at: 1391 2244 3278 1391 Size: 853 1034 4072 Fragments arranged by size: 4072 1034 853 BbeI G_GCGC'C Cuts at: 2193 2288 2193 Size: 95 5864 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4988 BbvCI CC'TCA_GC Cuts at: 2066 2066 Size: 5959 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4150 4184 1903 Size: 34 2213 34 3678 Fragments arranged by size: 3678 2213 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 2422 2551 1548 Size: 874 129 4956 Fragments arranged by size: 4956 874 129 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2166 4570 136 Size: 107 122 71 631 605 494 2404 1525 Fragments arranged by size: 2404 1525 631 605 494 122 107 71 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 3376 3376 Size: 5959 Bme1580I G_kGCm'C Cuts at: 1790 4020 5176 1790 Size: 2230 1156 2573 Fragments arranged by size: 2573 2230 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5959 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3690 4648 442 Size: 416 53 83 399 24 2273 958 1753 Fragments arranged by size: 2273 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2111 2298 2979 4619 1239 Size: 872 187 681 1640 2579 Fragments arranged by size: 2579 1640 872 681 187 Bpu10I CC'TnA_GC Cuts at: 2066 2919 2066 Size: 853 5106 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2481 4084 4949 5247 2481 Size: 1603 865 298 3193 Fragments arranged by size: 3193 1603 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4622 882 Size: 617 3123 2219 Fragments arranged by size: 3123 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3750 493 Size: 711 2546 2702 Fragments arranged by size: 2702 2546 711 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2190 2285 3766 4148 Size: 53 83 186 936 657 95 1481 382 Cuts at: 4148 4783 275 Size: 635 1451 Fragments arranged by size: 1481 1451 936 657 635 382 186 95 83 53 BsaWI w'CCGG_w Cuts at: 2482 4392 4790 5064 5211 5622 2482 Size: 1910 398 274 147 411 2819 Fragments arranged by size: 2819 1910 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2477 2507 1178 Size: 30 165 30 1074 30 4630 Fragments arranged by size: 4630 1074 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2252 2285 2288 2291 1186 Size: 1066 33 3 3 4854 Fragments arranged by size: 4854 1066 33 3 3 BseYI C'CCAG_C Cuts at: 2213 5162 5658 2213 Size: 2949 496 2514 Fragments arranged by size: 2949 2514 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2373 3036 1163 Size: 573 637 663 4086 Fragments arranged by size: 4086 663 637 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3409 4170 5198 1540 Size: 275 186 1408 761 1028 2301 Fragments arranged by size: 2301 1408 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2734 4020 4105 5176 2734 Size: 1286 85 1071 3517 Fragments arranged by size: 3517 1286 1071 85 BsmI GAATG_Cn' Cuts at: 3470 3563 3470 Size: 93 5866 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2244 2734 3278 4020 4105 5176 1391 Size: 399 454 490 544 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 544 490 454 399 85 BspEI T'CCGG_A Cuts at: 2482 5622 2482 Size: 3140 2819 BspHI T'CATG_A Cuts at: 3848 5578 3848 Size: 1730 4229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2094 3314 844 Size: 1250 1220 3489 Fragments arranged by size: 3489 1250 1220 BsrBI CCG'CTC Cuts at: 1394 1823 3846 1394 Size: 429 2023 3507 Fragments arranged by size: 3507 2023 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2913 3135 4454 4628 65 Size: 1183 1665 222 1319 174 1396 Fragments arranged by size: 1665 1396 1319 1183 222 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4603 4790 1700 Size: 110 2793 187 2869 Fragments arranged by size: 2869 2793 187 110 BsrGI T'GTAC_A Cuts at: 96 2685 96 Size: 2589 3370 BssHII G'CGCG_C Cuts at: 1925 2150 1925 Size: 225 5734 BssSI C'ACGA_G Cuts at: 4019 5031 4019 Size: 1012 4947 BstAPI GCAn_nnn'nTGC Cuts at: 794 3095 794 Size: 2301 3658 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 2752 1184 Size: 1568 4391 BstXI CCAn_nnnn'nTGG Cuts at: 3107 3107 Size: 5959 BstYI r'GATC_y Cuts at: 1972 2016 2479 3289 3656 4041 4058 5510 5596 Size: 44 463 810 367 385 17 1452 86 Cuts at: 5596 5608 5954 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 810 463 385 367 346 86 44 17 12 BstZ17I GTA'TAC Cuts at: 2893 2893 Size: 5959 BtgI C'CryG_G Cuts at: 513 1065 2079 2289 513 Size: 552 1014 210 4183 Fragments arranged by size: 4183 1014 552 210 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3175 487 Size: 683 56 1949 3271 Fragments arranged by size: 3271 1949 683 56 BtsI GCAGTG_nn' Cuts at: 2712 2822 3252 3475 4269 4289 2712 Size: 110 430 223 794 20 4382 Fragments arranged by size: 4382 794 430 223 110 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2158 3406 4295 Size: 54 1425 50 275 18 328 1248 889 Cuts at: 4295 5924 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1248 889 328 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3000 3889 1353 Size: 1647 889 3423 Fragments arranged by size: 3423 1647 889 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4546 4920 5066 387 Size: 1064 138 2957 374 146 1280 Fragments arranged by size: 2957 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2298 2743 2979 4022 4619 5406 Size: 393 479 187 445 236 1043 597 787 Cuts at: 5406 5674 1239 Size: 268 1524 Fragments arranged by size: 1524 1043 787 597 479 445 393 268 236 187 EcoNI CCTnn'n_nnAGG Cuts at: 2123 2123 Size: 5959 EcoO109I rG'GnC_Cy Cuts at: 2172 2182 3161 2172 Size: 10 979 4970 Fragments arranged by size: 4970 979 10 EcoRI G'AATT_C Cuts at: 3268 3646 3268 Size: 378 5581 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 2193 2288 5106 1678 Size: 515 95 2818 2531 Fragments arranged by size: 2818 2531 515 95 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2378 2392 2410 2424 2477 Size: 32 135 32 806 14 18 14 53 Cuts at: 2477 2509 2895 2927 4600 4632 4674 4706 1373 Size: 32 386 32 1673 32 42 32 2626 Fragments arranged by size: 2626 1673 806 386 135 53 42 32 32 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 2227 3303 3549 669 Size: 988 281 289 1076 246 3079 Fragments arranged by size: 3079 1076 988 289 281 246 HindIII A'AGCT_T Cuts at: 748 2358 2809 748 Size: 1610 451 3898 Fragments arranged by size: 3898 1610 451 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KasI G'GCGC_C Cuts at: 2189 2284 2189 Size: 95 5864 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2277 2608 5073 5257 1305 Size: 972 331 2465 184 2007 Fragments arranged by size: 2465 2007 972 331 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4137 Fragments arranged by size: 4137 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3917 4276 4435 518 Size: 648 2751 359 159 2042 Fragments arranged by size: 2751 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2081 2195 2291 3375 4052 5200 5445 1606 Size: 475 114 96 1084 677 1148 245 2120 Fragments arranged by size: 2120 1148 1084 677 475 245 114 96 NarI GG'CG_CC Cuts at: 2190 2285 2190 Size: 95 5864 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5407 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 2546 3317 4862 1132 Size: 97 1317 771 1545 2229 Fragments arranged by size: 2229 1545 1317 771 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5959 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5959 PfoI T'CCnGG_A Cuts at: 2396 2396 Size: 5959 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2172 2182 3161 2172 Size: 10 979 4970 Fragments arranged by size: 4970 979 10 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 2364 3529 2364 Size: 1165 4794 PspXI vC'TCGA_Gb Cuts at: 3084 3084 Size: 5959 PstI C_TGCA'G Cuts at: 830 2046 2106 2124 3259 3311 830 Size: 1216 60 18 1135 52 3478 Fragments arranged by size: 3478 1216 1135 60 52 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 SacII CC_GC'GG Cuts at: 2082 2292 2082 Size: 210 5749 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 3050 4207 5919 3050 Size: 1157 1712 3090 Fragments arranged by size: 3090 1712 1157 SfcI C'TryA_G Cuts at: 826 1045 2042 2102 2120 3255 3307 4442 5123 Size: 219 997 60 18 1135 52 1135 681 Cuts at: 5123 5314 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1135 997 681 219 191 60 52 18 SfoI GGC'GCC Cuts at: 2191 2286 2191 Size: 95 5864 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2496 3084 4063 4964 5226 820 Size: 197 926 553 588 979 901 262 1553 Fragments arranged by size: 1553 979 926 901 588 553 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5959 StyI C'CwwG_G Cuts at: 513 1065 3398 513 Size: 552 2333 3074 Fragments arranged by size: 3074 2333 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2710 3986 4003 4156 4341 4786 325 Size: 1255 1130 1276 17 153 185 445 1498 Fragments arranged by size: 1498 1276 1255 1130 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2685 3048 4205 Size: 274 80 33 51 1443 708 363 1157 Cuts at: 4205 5917 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1157 708 363 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2814 2846 5919 5951 2814 Size: 32 3073 32 2822 Fragments arranged by size: 3073 2822 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 3084 1943 Size: 1141 4818 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 3153 4088 3153 Size: 935 5024 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI