(Circular) (Six-base) MAPSORT of: pFN21AB3078.seq Check: 4486 from: 1 to: 7528 pFN21AB3078 7528 bp With 182 enzymes: SgfI * September 11, 2009 17:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2869 2869 Size: 7528 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5338 278 Size: 53 83 186 936 3802 2468 Fragments arranged by size: 3802 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4092 4871 1937 Size: 2155 779 4594 Fragments arranged by size: 4594 2155 779 Acc65I G'GTAC_C Cuts at: 4849 4849 Size: 7528 AclI AA'CG_TT Cuts at: 1563 5655 6028 1563 Size: 4092 373 3063 Fragments arranged by size: 4092 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2585 2753 5591 6975 7243 1632 Size: 953 168 2838 1384 268 1917 Fragments arranged by size: 2838 1917 1384 953 268 168 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7331 AflIII A'CryG_T Cuts at: 1203 2817 4789 6427 7404 1203 Size: 1614 1972 1638 977 1327 Fragments arranged by size: 1972 1638 1614 1327 977 AgeI A'CCGG_T Cuts at: 6359 6359 Size: 7528 AhdI GACnn_n'nnGTC Cuts at: 6257 6257 Size: 7528 AleI CACnn'nnGTG Cuts at: 1166 4340 1166 Size: 3174 4354 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7528 AlwNI CAG_nnn'CTG Cuts at: 3675 3793 4755 5271 6843 3675 Size: 118 962 516 1572 4360 Fragments arranged by size: 4360 1572 962 516 118 ApaI G_GGCC'C Cuts at: 3057 3057 Size: 7528 ApaLI G'TGCA_C Cuts at: 2882 5585 6741 2882 Size: 2703 1156 3669 Fragments arranged by size: 3669 2703 1156 ApoI r'AATT_y Cuts at: 1493 3284 4837 5063 5215 7324 1493 Size: 1791 1553 226 152 2109 1697 Fragments arranged by size: 2109 1791 1697 1553 226 152 AseI AT'TA_AT Cuts at: 160 1032 6082 160 Size: 872 5050 1606 Fragments arranged by size: 5050 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7528 AvaI C'yCGr_G Cuts at: 1870 1943 2033 2114 3476 4143 4819 4853 1870 Size: 73 90 81 1362 667 676 34 4545 Fragments arranged by size: 4545 1362 676 667 90 81 73 34 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3817 3850 3817 Size: 33 7495 BamHI G'GATC_C Cuts at: 3156 3383 3626 4858 5225 3156 Size: 227 243 1232 367 5459 Fragments arranged by size: 5459 1232 367 243 227 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2110 2138 3147 4849 6304 Size: 325 217 627 323 28 1009 1702 1455 Cuts at: 6304 618 Size: 1842 Fragments arranged by size: 1842 1702 1455 1009 627 325 323 217 28 BanII G_rGCy'C Cuts at: 1391 2024 2967 2975 3057 4506 4617 4847 1391 Size: 633 943 8 82 1449 111 230 4072 Fragments arranged by size: 4072 1449 943 633 230 111 82 8 BbeI G_GCGC'C Cuts at: 2114 2142 2114 Size: 28 7500 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6557 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4060 4094 5719 5753 1903 Size: 34 2123 34 1625 34 3678 Fragments arranged by size: 3678 2123 1625 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3845 5420 6630 3845 Size: 1575 1210 4743 Fragments arranged by size: 4743 1575 1210 BclI T'GATC_A Cuts at: 1548 3483 1548 Size: 1935 5593 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4371 6139 136 Size: 107 122 71 631 605 2699 1768 1525 Fragments arranged by size: 2699 1768 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3432 7523 3432 Size: 4091 3437 BlpI GC'TnA_GC Cuts at: 4945 4945 Size: 7528 Bme1580I G_kGCm'C Cuts at: 1790 2603 2886 3057 5589 6745 1790 Size: 813 283 171 2532 1156 2573 Fragments arranged by size: 2573 2532 1156 813 283 171 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7528 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5259 6217 442 Size: 416 53 83 399 24 3842 958 1753 Fragments arranged by size: 3842 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7528 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2716 3605 2716 Size: 889 6639 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2673 3371 3437 3751 3959 4388 4641 4670 Size: 1434 698 66 314 208 429 253 29 Cuts at: 4670 6188 1239 Size: 1518 2579 Fragments arranged by size: 2579 1518 1434 698 429 314 253 208 66 29 Bpu10I CC'TnA_GC Cuts at: 2969 2969 Size: 7528 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2558 5653 6518 6816 2558 Size: 3095 865 298 3270 Fragments arranged by size: 3270 3095 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3212 4028 4822 6191 882 Size: 617 1713 816 794 1369 2219 Fragments arranged by size: 2219 1713 1369 816 794 617 BsaAI yAC'GTr Cuts at: 493 1204 5319 493 Size: 711 4115 2702 Fragments arranged by size: 4115 2702 711 BsaBI GATnn'nnATC Cuts at: 4584 7522 4584 Size: 2938 4590 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2111 2139 2403 4737 Size: 53 83 186 936 578 28 264 2334 Cuts at: 4737 5335 5717 6352 275 Size: 598 382 635 1451 Fragments arranged by size: 2334 1451 936 635 598 578 382 264 186 83 53 28 BsaWI w'CCGG_w Cuts at: 2913 3255 3522 5961 6359 6633 6780 7191 2913 Size: 342 267 2439 398 274 147 411 3250 Fragments arranged by size: 3250 2439 411 398 342 274 267 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2804 2834 1178 Size: 30 165 30 1401 30 5872 Fragments arranged by size: 5872 1401 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2825 1186 Size: 1639 5889 BseYI C'CCAG_C Cuts at: 2127 2156 3056 3187 3904 4590 6731 7227 2127 Size: 29 900 131 717 686 2141 496 2428 Fragments arranged by size: 2428 2141 900 717 686 496 131 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2480 4181 1163 Size: 573 744 1701 4510 Fragments arranged by size: 4510 1701 744 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2150 3702 4978 5739 6767 1540 Size: 275 186 149 1552 1276 761 1028 2301 Fragments arranged by size: 2301 1552 1276 1028 761 275 186 149 BsiHKAI G_wGCw'C Cuts at: 2024 2238 2886 2975 3945 4506 4544 5589 5674 Size: 214 648 89 970 561 38 1045 85 Cuts at: 5674 6745 2024 Size: 1071 2807 Fragments arranged by size: 2807 1071 1045 970 648 561 214 89 85 38 BsmI GAATG_Cn' Cuts at: 5039 5132 5039 Size: 93 7435 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2238 2603 2886 2967 2975 3057 Size: 399 234 214 365 283 81 8 82 Cuts at: 3057 3945 4506 4544 4617 4847 5589 5674 6745 Size: 888 561 38 73 230 742 85 1071 Cuts at: 6745 1391 Size: 2174 Fragments arranged by size: 2174 1071 888 742 561 399 365 283 234 230 214 85 82 81 73 38 8 BspEI T'CCGG_A Cuts at: 2913 3255 7191 2913 Size: 342 3936 3250 Fragments arranged by size: 3936 3250 342 BspHI T'CATG_A Cuts at: 5417 7147 5417 Size: 1730 5798 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2313 2869 4883 844 Size: 1469 556 2014 3489 Fragments arranged by size: 3489 2014 1469 556 BsrBI CCG'CTC Cuts at: 1394 1823 2170 2194 5415 1394 Size: 429 347 24 3221 3507 Fragments arranged by size: 3507 3221 429 347 24 BsrDI GCAATG_nn' Cuts at: 65 1248 2427 6023 6197 65 Size: 1183 1179 3596 174 1396 Fragments arranged by size: 3596 1396 1183 1179 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3144 6172 6359 1700 Size: 110 1334 3028 187 2869 Fragments arranged by size: 3028 2869 1334 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7528 BssHII G'CGCG_C Cuts at: 1925 2066 1925 Size: 141 7387 BssSI C'ACGA_G Cuts at: 5588 6600 5588 Size: 1012 6516 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7528 BstBI TT'CG_AA Cuts at: 6332 6332 Size: 7528 BstEII G'GTnAC_C Cuts at: 1184 4333 4438 1184 Size: 3149 105 4274 Fragments arranged by size: 4274 3149 105 BstXI CCAn_nnnn'nTGG Cuts at: 2206 3415 2206 Size: 1209 6319 BstYI r'GATC_y Cuts at: 1972 2265 3156 3383 3432 3626 4633 4858 5225 Size: 293 891 227 49 194 1007 225 367 Cuts at: 5225 5610 5627 7079 7165 7177 7523 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1007 891 385 367 346 293 227 225 194 86 49 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2015 2244 2934 3067 3120 3687 Size: 552 939 11 229 690 133 53 567 Cuts at: 3687 513 Size: 4354 Fragments arranged by size: 4354 939 690 567 552 229 133 53 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3082 4376 487 Size: 683 56 1856 1294 3639 Fragments arranged by size: 3639 1856 1294 683 56 BtsI GCAGTG_nn' Cuts at: 3107 3128 5044 5838 5858 3107 Size: 21 1916 794 20 4777 Fragments arranged by size: 4777 1916 794 21 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7528 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7493 DraI TTT'AAA Cuts at: 4566 4832 4566 Size: 266 7262 DraIII CAC_nnn'GTG Cuts at: 2325 2325 Size: 7528 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6535 809 Size: 507 5219 1802 Fragments arranged by size: 5219 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2147 2208 2253 Size: 54 1425 50 275 18 317 61 45 Cuts at: 2253 2761 3339 3489 4975 5864 7493 8 Size: 508 578 150 1486 889 1629 43 Fragments arranged by size: 1629 1486 1425 889 578 508 317 275 150 61 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2147 4975 1537 Size: 275 335 2828 4090 Fragments arranged by size: 4090 2828 335 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3509 4428 5458 1353 Size: 2156 919 1030 3423 Fragments arranged by size: 3423 2156 1030 919 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3917 6115 6489 6635 387 Size: 1064 138 2328 2198 374 146 1280 Fragments arranged by size: 2328 2198 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2022 2973 4504 2022 Size: 951 1531 5046 Fragments arranged by size: 5046 1531 951 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2585 2673 2753 3371 3437 3751 3959 Size: 393 953 88 80 618 66 314 208 Cuts at: 3959 4388 4641 4670 5591 6188 6975 7243 1239 Size: 429 253 29 921 597 787 268 1524 Fragments arranged by size: 1524 953 921 787 618 597 429 393 314 268 253 208 88 80 66 29 EcoO109I rG'GnC_Cy Cuts at: 2118 3053 3672 4372 2118 Size: 935 619 700 5274 Fragments arranged by size: 5274 935 700 619 EcoRI G'AATT_C Cuts at: 4837 5215 4837 Size: 378 7150 EcoRV GAT'ATC Cuts at: 3919 3919 Size: 7528 FspI TGC'GCA Cuts at: 6034 6034 Size: 7528 HaeII r_GCGC'y Cuts at: 1678 2114 2142 6675 1678 Size: 436 28 4533 2531 Fragments arranged by size: 4533 2531 436 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3535 3567 3618 3650 3903 Size: 32 135 32 1963 32 51 32 253 Cuts at: 3903 3935 4637 4669 4800 4832 6169 6201 6243 Size: 32 702 32 131 32 1337 32 42 Cuts at: 6243 6275 1373 Size: 32 2626 Fragments arranged by size: 2626 1963 1337 702 253 135 131 51 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4872 5118 669 Size: 988 281 2934 246 3079 Fragments arranged by size: 3079 2934 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7528 HpaI GTT'AAC Cuts at: 5118 5118 Size: 7528 KasI G'GCGC_C Cuts at: 2110 2138 2110 Size: 28 7500 KpnI G_GTAC'C Cuts at: 4853 4853 Size: 7528 MfeI C'AATT_G Cuts at: 5127 5127 Size: 7528 MluI A'CGCG_T Cuts at: 7404 7404 Size: 7528 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2727 6642 6826 1305 Size: 1422 3915 184 2007 Fragments arranged by size: 3915 2007 1422 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2210 2255 2763 3491 10 Size: 54 1425 343 378 45 508 728 4047 Fragments arranged by size: 4047 1425 728 508 378 343 54 45 MslI CAynn'nnrTG Cuts at: 518 1166 2951 3542 3959 4082 4340 4788 5486 Size: 648 1785 591 417 123 258 448 698 Cuts at: 5486 5845 6004 518 Size: 359 159 2042 Fragments arranged by size: 2042 1785 698 648 591 448 417 359 258 159 123 MspA1I CmG'CkG Cuts at: 1606 2017 2095 2907 3154 3424 4127 4450 4743 Size: 411 78 812 247 270 703 323 293 Cuts at: 4743 4944 5621 6769 7014 1606 Size: 201 677 1148 245 2120 Fragments arranged by size: 2120 1148 812 703 677 411 323 293 270 247 245 201 78 NaeI GCC'GGC Cuts at: 3146 3146 Size: 7528 NarI GG'CG_CC Cuts at: 2111 2139 2111 Size: 28 7500 NcoI C'CATG_G Cuts at: 513 1065 2004 2934 3120 513 Size: 552 939 930 186 4921 Fragments arranged by size: 4921 939 930 552 186 NdeI CA'TA_TG Cuts at: 387 3960 387 Size: 3573 3955 NgoMIV G'CCGG_C Cuts at: 3144 3144 Size: 7528 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7528 NotI GC'GGCC_GC Cuts at: 4975 4975 Size: 7528 NspI r_CATG'y Cuts at: 1132 1229 2821 3137 3817 4629 4793 4886 6431 Size: 97 1592 316 680 812 164 93 1545 Cuts at: 6431 1132 Size: 2229 Fragments arranged by size: 2229 1592 1545 812 680 316 164 97 93 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7528 PciI A'CATG_T Cuts at: 2817 4789 6427 2817 Size: 1972 1638 3918 Fragments arranged by size: 3918 1972 1638 PflMI CCAn_nnn'nTGG Cuts at: 1108 2389 2779 3187 1108 Size: 1281 390 408 5449 Fragments arranged by size: 5449 1281 408 390 PfoI T'CCnGG_A Cuts at: 3159 3414 3936 3159 Size: 255 522 6751 Fragments arranged by size: 6751 522 255 PmeI GTTT'AAAC Cuts at: 4832 4832 Size: 7528 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5597 5629 7110 7142 5597 Size: 32 1481 32 5983 Fragments arranged by size: 5983 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3672 3672 Size: 7528 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7528 PsiI TTA'TAA Cuts at: 5098 5098 Size: 7528 PspOMI G'GGCC_C Cuts at: 3053 3053 Size: 7528 PstI C_TGCA'G Cuts at: 830 3114 4199 4455 4880 830 Size: 2284 1085 256 425 3478 Fragments arranged by size: 3478 2284 1085 425 256 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7528 PvuII CAG'CTG Cuts at: 3424 4450 4743 3424 Size: 1026 293 6209 Fragments arranged by size: 6209 1026 293 RsrII CG'GwC_CG Cuts at: 2526 2526 Size: 7528 SacI G_AGCT'C Cuts at: 2024 2975 4506 2024 Size: 951 1531 5046 Fragments arranged by size: 5046 1531 951 SacII CC_GC'GG Cuts at: 2018 2018 Size: 7528 SalI G'TCGA_C Cuts at: 1936 4870 1936 Size: 2934 4594 SbfI CC_TGCA'GG Cuts at: 4880 4880 Size: 7528 ScaI AGT'ACT Cuts at: 5776 7488 5776 Size: 1712 5816 SfcI C'TryA_G Cuts at: 826 1045 3110 3448 4195 4376 4451 4595 4638 Size: 219 2065 338 747 181 75 144 43 Cuts at: 4638 4876 6011 6692 6883 826 Size: 238 1135 681 191 1471 Fragments arranged by size: 2065 1471 1135 747 681 338 238 219 191 181 144 75 43 SfoI GGC'GCC Cuts at: 2112 2140 2112 Size: 28 7500 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7528 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7528 SmaI CCC'GGG Cuts at: 1872 2035 2116 4145 4855 1872 Size: 163 81 2029 710 4545 Fragments arranged by size: 4545 2029 710 163 81 SmlI C'TyrA_G Cuts at: 820 1017 1943 2573 5632 6533 6795 820 Size: 197 926 630 3059 901 262 1553 Fragments arranged by size: 3059 1553 926 901 630 262 197 SnaBI TAC'GTA Cuts at: 493 5319 493 Size: 4826 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7528 SphI G_CATG'C Cuts at: 1132 3137 3817 4886 1132 Size: 2005 680 1069 3774 Fragments arranged by size: 3774 2005 1069 680 SrfI GCCC'GGGC Cuts at: 2035 2035 Size: 7528 SspI AAT'ATT Cuts at: 5 52 5452 5 Size: 47 5400 2081 Fragments arranged by size: 5400 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7528 StyI C'CwwG_G Cuts at: 513 1065 2004 2199 2211 2443 2758 2934 3120 Size: 552 939 195 12 232 315 176 186 Cuts at: 3120 3379 3577 4967 513 Size: 259 198 1390 3074 Fragments arranged by size: 3074 1390 939 552 315 259 232 198 195 186 176 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3971 4321 5555 5572 5725 5910 6355 Size: 1255 2391 350 1234 17 153 185 445 Cuts at: 6355 325 Size: 1498 Fragments arranged by size: 2391 1498 1255 1234 445 350 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3027 3327 3885 Size: 274 80 33 51 1443 1050 300 558 Cuts at: 3885 4074 5774 7486 96 Size: 189 1700 1712 138 Fragments arranged by size: 1712 1700 1443 1050 558 300 274 189 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4788 4820 7488 7520 4788 Size: 32 2668 32 4796 Fragments arranged by size: 4796 2668 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7528 XbaI T'CTAG_A Cuts at: 4864 4864 Size: 7528 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4729 4729 Size: 7528 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7528 XmaI C'CCGG_G Cuts at: 1870 2033 2114 4143 4853 1870 Size: 163 81 2029 710 4545 Fragments arranged by size: 4545 2029 710 163 81 XmnI GAAnn'nnTTC Cuts at: 2351 5657 2351 Size: 3306 4222 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5336 276 Size: 53 83 186 936 3802 2468 Fragments arranged by size: 3802 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI SanDI SapI SexAI SfiI SwaI