Restriction map for
pFN21AB3048
(Linear) (Six-base) MAPSORT of: KIBB3048.seq Check: 5318 from: 1 to: 4026
KIBB3048.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3802 4026
Size: 3802 224
AatII G_ACGT'C
Cuts at: 0 1760 4026
Size: 1760 2266
AccI GT'mk_AC
Cuts at: 0 662 3381 3425 3661 4026
Size: 662 2719 44 236 365
Fragments arranged by size:
2719 662 365 236 44
Acc65I G'GTAC_C
Cuts at: 0 778 1823 2186 4026
Size: 778 1045 363 1840
Fragments arranged by size:
1840 1045 778 363
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 1448 2148 3128 3583 3633 4026
Size: 515 933 700 980 455 50 393
Fragments arranged by size:
980 933 700 515 455 393 50
AfeI AGC'GCT
Cuts at: 0 734 4026
Size: 734 3292
AflIII A'CryG_T
Cuts at: 0 2042 2458 2584 3464 4026
Size: 2042 416 126 880 562
Fragments arranged by size:
2042 880 562 416 126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1542 3159 4026
Size: 1542 1617 867
Fragments arranged by size:
1617 1542 867
AlwNI CAG_nnn'CTG
Cuts at: 0 2936 4026
Size: 2936 1090
ApaI G_GGCC'C
Cuts at: 0 1055 1156 4026
Size: 1055 101 2870
Fragments arranged by size:
2870 1055 101
ApaLI G'TGCA_C
Cuts at: 0 2169 4026
Size: 2169 1857
ApoI r'AATT_y
Cuts at: 0 291 451 1905 3999 4026
Size: 291 160 1454 2094 27
Fragments arranged by size:
2094 1454 291 160 27
AseI AT'TA_AT
Cuts at: 0 902 2396 2471 4026
Size: 902 1494 75 1555
Fragments arranged by size:
1555 1494 902 75
AvaI C'yCGr_G
Cuts at: 0 1223 4026
Size: 1223 2803
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2170 2203 4026
Size: 2170 33 1823
Fragments arranged by size:
2170 1823 33
BamHI G'GATC_C
Cuts at: 0 996 4026
Size: 996 3030
BanI G'GyrC_C
Cuts at: 0 328 778 1823 2186 4026
Size: 328 450 1045 363 1840
Fragments arranged by size:
1840 1045 450 363 328
BanII G_rGCy'C
Cuts at: 0 1055 1156 2161 2284 2495 3325 4026
Size: 1055 101 1005 123 211 830 701
Fragments arranged by size:
1055 1005 830 701 211 123 101
BbsI GAAGACnn'nnnn_
Cuts at: 0 556 616 1086 2225 4026
Size: 556 60 470 1139 1801
Fragments arranged by size:
1801 1139 556 470 60
BbvCI CC'TCA_GC
Cuts at: 0 3728 4026
Size: 3728 298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 956 990 4026
Size: 956 34 3036
Fragments arranged by size:
3036 956 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 829 3816 4026
Size: 829 2987 210
Fragments arranged by size:
2987 829 210
BglI GCCn_nnn'nGGC
Cuts at: 0 542 2060 4026
Size: 542 1518 1966
Fragments arranged by size:
1966 1518 542
BlpI GC'TnA_GC
Cuts at: 0 3584 4026
Size: 3584 442
Bme1580I G_kGCm'C
Cuts at: 0 286 1055 1156 1775 2173 3552 4026
Size: 286 769 101 619 398 1379 474
Fragments arranged by size:
1379 769 619 474 398 286 101
BmgBI CAC'GTC
Cuts at: 0 660 4026
Size: 660 3366
BmrI ACTGGGnnnn_n'
Cuts at: 0 313 2843 3278 4026
Size: 313 2530 435 748
Fragments arranged by size:
2530 748 435 313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1325 3113 3650 4026
Size: 1325 1788 537 376
Fragments arranged by size:
1788 1325 537 376
Bpu10I CC'TnA_GC
Cuts at: 0 3728 4026
Size: 3728 298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1336 1454 4026
Size: 1336 118 2572
Fragments arranged by size:
2572 1336 118
BsaI GGTCTCn'nnnn_
Cuts at: 0 2324 4026
Size: 2324 1702
BsaAI yAC'GTr
Cuts at: 0 2585 3465 3951 4026
Size: 2585 880 486 75
Fragments arranged by size:
2585 880 486 75
BsaBI GATnn'nnATC
Cuts at: 0 2879 3812 4026
Size: 2879 933 214
Fragments arranged by size:
2879 933 214
BsaHI Gr'CG_yC
Cuts at: 0 1757 2927 3612 3635 4026
Size: 1757 1170 685 23 391
Fragments arranged by size:
1757 1170 685 391 23
BsaWI w'CCGG_w
Cuts at: 0 1799 2076 2427 3875 4026
Size: 1799 277 351 1448 151
Fragments arranged by size:
1799 1448 351 277 151
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 431 461 1386 1416 1624 1654 2058 2088
Size: 431 30 925 30 208 30 404 30
Cuts at: 2088 4026
Size: 1938
Fragments arranged by size:
1938 925 431 404 208 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1726 2399 3730 4026
Size: 1726 673 1331 296
Fragments arranged by size:
1726 1331 673 296
BseYI C'CCAG_C
Cuts at: 0 359 1331 1689 3440 4026
Size: 359 972 358 1751 586
Fragments arranged by size:
1751 972 586 359 358
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 279 1250 1920 2286 3960 4026
Size: 99 180 971 670 366 1674 66
Fragments arranged by size:
1674 971 670 366 180 99 66
BsiEI CG_ry'CG
Cuts at: 0 3388 4026
Size: 3388 638
BsiHKAI G_wGCw'C
Cuts at: 0 2173 2256 4026
Size: 2173 83 1770
Fragments arranged by size:
2173 1770 83
BsiWI C'GTAC_G
Cuts at: 0 2581 4026
Size: 2581 1445
BsmI GAATG_Cn'
Cuts at: 0 354 859 1747 2236 2359 2911 2980 3148
Size: 354 505 888 489 123 552 69 168
Cuts at: 3148 3214 3739 3862 3900 4026
Size: 66 525 123 38 126
Fragments arranged by size:
888 552 525 505 489 354 168 126
123 123 69 66 38
Bsp1286I G_dGCh'C
Cuts at: 0 286 1055 1156 1775 2161 2173 2256 2284
Size: 286 769 101 619 386 12 83 28
Cuts at: 2284 2495 3325 3552 4026
Size: 211 830 227 474
Fragments arranged by size:
830 769 619 474 386 286 227 211
101 83 28 12
BspEI T'CCGG_A
Cuts at: 0 2076 2427 4026
Size: 2076 351 1599
Fragments arranged by size:
2076 1599 351
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 121 636 982 3802 4026
Size: 121 515 346 2820 224
Fragments arranged by size:
2820 515 346 224 121
BsrBI CCG'CTC
Cuts at: 0 48 2846 4026
Size: 48 2798 1180
Fragments arranged by size:
2798 1180 48
BsrFI r'CCGG_y
Cuts at: 0 692 3387 3473 3614 4026
Size: 692 2695 86 141 412
Fragments arranged by size:
2695 692 412 141 86
BsrGI T'GTAC_A
Cuts at: 0 2983 4026
Size: 2983 1043
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3357 4026
Size: 3357 669
BstYI r'GATC_y
Cuts at: 0 314 996 1915 4026
Size: 314 682 919 2111
Fragments arranged by size:
2111 919 682 314
BstZ17I GTA'TAC
Cuts at: 0 3382 3662 4026
Size: 3382 280 364
Fragments arranged by size:
3382 364 280
Bsu36I CC'TnA_GG
Cuts at: 0 1747 4026
Size: 1747 2279
BtgI C'CryG_G
Cuts at: 0 324 668 953 3350 4026
Size: 324 344 285 2397 676
Fragments arranged by size:
2397 676 344 324 285
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3875 4026
Size: 3875 151
BtsI GCAGTG_nn'
Cuts at: 0 1524 1903 2146 2269 2845 3259 3550 4026
Size: 1524 379 243 123 576 414 291 476
Fragments arranged by size:
1524 576 476 414 379 291 243 123
ClaI AT'CG_AT
Cuts at: 0 966 4026
Size: 966 3060
DraIII CAC_nnn'GTG
Cuts at: 0 724 1162 3509 4026
Size: 724 438 2347 517
Fragments arranged by size:
2347 724 517 438
EaeI y'GGCC_r
Cuts at: 0 69 203 252 690 1703 2577 3300 3827
Size: 69 134 49 438 1013 874 723 527
Cuts at: 3827 4026
Size: 199
Fragments arranged by size:
1013 874 723 527 438 199 134 69
49
EarI CTCTTCn'nnn_
Cuts at: 0 1037 1190 4026
Size: 1037 153 2836
Fragments arranged by size:
2836 1037 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1261 4026
Size: 1261 2765
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 1325 1448 2148 3113 3128 3583 3633
Size: 515 810 123 700 965 15 455 50
Cuts at: 3633 3650 4026
Size: 17 376
Fragments arranged by size:
965 810 700 515 455 376 123 50
17 15
EcoO109I rG'GnC_Cy
Cuts at: 0 1051 1052 1152 1209 1793 2408 2783 4026
Size: 1051 1 100 57 584 615 375 1243
Fragments arranged by size:
1243 1051 615 584 375 100 57 1
EcoRI G'AATT_C
Cuts at: 0 1905 4026
Size: 1905 2121
EcoRV GAT'ATC
Cuts at: 0 1509 2346 4026
Size: 1509 837 1680
Fragments arranged by size:
1680 1509 837
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 102 4026
Size: 102 3924
FspI TGC'GCA
Cuts at: 0 2007 4026
Size: 2007 2019
HaeII r_GCGC'y
Cuts at: 0 736 4026
Size: 736 3290
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3149 3181 4026
Size: 3149 32 845
Fragments arranged by size:
3149 845 32
HincII GTy'rAC
Cuts at: 0 1327 1737 3645 4026
Size: 1327 410 1908 381
Fragments arranged by size:
1908 1327 410 381
HindIII A'AGCT_T
Cuts at: 0 497 1430 4026
Size: 497 933 2596
Fragments arranged by size:
2596 933 497
KpnI G_GTAC'C
Cuts at: 0 782 1827 2190 4026
Size: 782 1045 363 1836
Fragments arranged by size:
1836 1045 782 363
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1653 2048 4026
Size: 1653 395 1978
Fragments arranged by size:
1978 1653 395
MscI TGG'CCA
Cuts at: 0 254 1705 3302 4026
Size: 254 1451 1597 724
Fragments arranged by size:
1597 1451 724 254
MslI CAynn'nnrTG
Cuts at: 0 79 2249 3058 3452 3601 4026
Size: 79 2170 809 394 149 425
Fragments arranged by size:
2170 809 425 394 149 79
MspA1I CmG'CkG
Cuts at: 0 128 140 1335 1652 1820 2193 2653 2666
Size: 128 12 1195 317 168 373 460 13
Cuts at: 2666 3588 4026
Size: 922 438
Fragments arranged by size:
1195 922 460 438 373 317 168 128
13 12
NaeI GCC'GGC
Cuts at: 0 3475 3616 4026
Size: 3475 141 410
Fragments arranged by size:
3475 410 141
NcoI C'CATG_G
Cuts at: 0 324 953 3350 4026
Size: 324 629 2397 676
Fragments arranged by size:
2397 676 629 324
NdeI CA'TA_TG
Cuts at: 0 3624 4026
Size: 3624 402
NgoMIV G'CCGG_C
Cuts at: 0 3473 3614 4026
Size: 3473 141 412
Fragments arranged by size:
3473 412 141
NspI r_CATG'y
Cuts at: 0 391 2046 2462 3543 4026
Size: 391 1655 416 1081 483
Fragments arranged by size:
1655 1081 483 416 391
PasI CC'CwG_GG
Cuts at: 0 1644 4026
Size: 1644 2382
PciI A'CATG_T
Cuts at: 0 2042 2458 4026
Size: 2042 416 1568
Fragments arranged by size:
2042 1568 416
PflMI CCAn_nnn'nTGG
Cuts at: 0 325 849 2701 4026
Size: 325 524 1852 1325
Fragments arranged by size:
1852 1325 524 325
PfoI T'CCnGG_A
Cuts at: 0 701 1911 2848 3356 3779 4026
Size: 701 1210 937 508 423 247
Fragments arranged by size:
1210 937 701 508 423 247
PpuMI rG'GwC_Cy
Cuts at: 0 2408 2783 4026
Size: 2408 375 1243
Fragments arranged by size:
2408 1243 375
PshAI GACnn'nnGTC
Cuts at: 0 275 388 2930 3841 4026
Size: 275 113 2542 911 185
Fragments arranged by size:
2542 911 275 185 113
PsiI TTA'TAA
Cuts at: 0 869 4026
Size: 869 3157
PspOMI G'GGCC_C
Cuts at: 0 1051 1152 4026
Size: 1051 101 2874
Fragments arranged by size:
2874 1051 101
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 762 794 4026
Size: 762 32 3232
Fragments arranged by size:
3232 762 32
PstI C_TGCA'G
Cuts at: 0 2406 2574 3254 4026
Size: 2406 168 680 772
Fragments arranged by size:
2406 772 680 168
PvuII CAG'CTG
Cuts at: 0 128 140 1335 2193 2653 3588 4026
Size: 128 12 1195 858 460 935 438
Fragments arranged by size:
1195 935 858 460 438 128 12
SapI GCTCTTCn'nnn_
Cuts at: 0 1190 4026
Size: 1190 2836
SfcI C'TryA_G
Cuts at: 0 1572 2091 2402 2570 3250 4026
Size: 1572 519 311 168 680 776
Fragments arranged by size:
1572 776 680 519 311 168
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 542 4026
Size: 542 3484
SmaI CCC'GGG
Cuts at: 0 1225 4026
Size: 1225 2801
SmlI C'TyrA_G
Cuts at: 0 1315 1469 4026
Size: 1315 154 2557
Fragments arranged by size:
2557 1315 154
SphI G_CATG'C
Cuts at: 0 3543 4026
Size: 3543 483
StuI AGG'CCT
Cuts at: 0 1147 3532 4026
Size: 1147 2385 494
Fragments arranged by size:
2385 1147 494
StyI C'CwwG_G
Cuts at: 0 119 324 591 953 1190 3350 3638 4026
Size: 119 205 267 362 237 2160 288 388
Fragments arranged by size:
2160 388 362 288 267 237 205 119
TatI w'GTAC_w
Cuts at: 0 1492 1618 2419 2819 2983 3340 4026
Size: 1492 126 801 400 164 357 686
Fragments arranged by size:
1492 801 686 400 357 164 126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 431 463 1624 1656 2112 2144 4026
Size: 431 32 1161 32 456 32 1882
Fragments arranged by size:
1882 1161 456 431 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 709 4026
Size: 709 3317
XmaI C'CCGG_G
Cuts at: 0 1223 4026
Size: 1223 2803
ZraI GAC'GTC
Cuts at: 0 1758 4026
Size: 1758 2268
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AlfI
AlwNI ApaI ApaLI ApoI AseI AvaI BaeI BamHI
BanI BanII BbsI BbvCI BcgI BciVI BglI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspMI BsrBI BsrFI
BsrGI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI DraIII EaeI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KpnI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuII SapI SfcI SfiI SmaI
SmlI SphI StuI StyI TatI TstI XcmI XmaI
ZraI
Enzymes that do not cut:
AclI AflII AgeI AhdI AleI AloI AscI AsiSI
AvrII BbeI BclI BfrBI BglII BmtI BplI BsmBI
BspHI BsrDI BssHII BssSI BstAPI BstBI BstEII CspCI
DraI DrdI EagI EcoICRI EcoNI FseI FspAI HpaI
KasI MfeI MluI NarI NheI NotI NruI NsiI
PacI PmeI PmlI PpiI PspXI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfoI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI TaqII Tth111I
XbaI XhoI XmnI