(Circular) (Six-base) MAPSORT of: pFN21AB3047.seq Check: 175 from: 1 to: 8146 pFN21AB3047 8146 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3667 3667 Size: 8146 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5956 278 Size: 53 83 186 936 4420 2468 Fragments arranged by size: 4420 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4904 5046 5489 1937 Size: 2967 142 443 4594 Fragments arranged by size: 4594 2967 443 142 Acc65I G'GTAC_C Cuts at: 5467 5467 Size: 8146 AclI AA'CG_TT Cuts at: 1563 2753 3298 6273 6646 1563 Size: 1190 545 2975 373 3063 Fragments arranged by size: 3063 2975 1190 545 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3132 4113 6209 7593 7861 1632 Size: 1500 981 2096 1384 268 1917 Fragments arranged by size: 2096 1917 1500 1384 981 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7949 AflIII A'CryG_T Cuts at: 1203 4593 4672 7045 8022 1203 Size: 3390 79 2373 977 1327 Fragments arranged by size: 3390 2373 1327 977 79 AgeI A'CCGG_T Cuts at: 6977 6977 Size: 8146 AhdI GACnn_n'nnGTC Cuts at: 6875 6875 Size: 8146 AleI CACnn'nnGTG Cuts at: 1166 3251 1166 Size: 2085 6061 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8146 AlwNI CAG_nnn'CTG Cuts at: 4936 5130 5889 7461 4936 Size: 194 759 1572 5621 Fragments arranged by size: 5621 1572 759 194 ApaI G_GGCC'C Cuts at: 4544 4544 Size: 8146 ApaLI G'TGCA_C Cuts at: 4467 6203 7359 4467 Size: 1736 1156 5254 Fragments arranged by size: 5254 1736 1156 ApoI r'AATT_y Cuts at: 1493 2018 2295 3715 3751 3902 4200 4236 5455 Size: 525 277 1420 36 151 298 36 1219 Cuts at: 5455 5681 5833 7942 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1420 1219 525 298 277 226 152 151 36 36 AseI AT'TA_AT Cuts at: 160 1032 4211 6700 160 Size: 872 3179 2489 1606 Fragments arranged by size: 3179 2489 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8146 AvaI C'yCGr_G Cuts at: 1870 1943 4326 5471 1870 Size: 73 2383 1145 4545 Fragments arranged by size: 4545 2383 1145 73 AvrII C'CTAG_G Cuts at: 2855 2855 Size: 8146 BamHI G'GATC_C Cuts at: 2308 5164 5476 5843 2308 Size: 2856 312 367 4611 Fragments arranged by size: 4611 2856 367 312 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5467 6922 618 Size: 325 217 627 3680 1455 1842 Fragments arranged by size: 3680 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2990 3191 4544 5415 5465 1391 Size: 1599 201 1353 871 50 4072 Fragments arranged by size: 4072 1599 1353 871 201 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4790 5106 928 Size: 971 2891 316 3968 Fragments arranged by size: 3968 2891 971 316 BbvCI CC'TCA_GC Cuts at: 3684 3684 Size: 8146 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6337 6371 1903 Size: 34 4400 34 3678 Fragments arranged by size: 4400 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4001 6038 7248 4001 Size: 2037 1210 4899 Fragments arranged by size: 4899 2037 1210 BclI T'GATC_A Cuts at: 1548 5358 1548 Size: 3810 4336 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6757 136 Size: 107 122 71 631 605 5085 1525 Fragments arranged by size: 5085 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2393 2531 4615 8141 2393 Size: 138 2084 3526 2398 Fragments arranged by size: 3526 2398 2084 138 BlpI GC'TnA_GC Cuts at: 5563 5563 Size: 8146 Bme1580I G_kGCm'C Cuts at: 1790 4471 4544 6207 7363 1790 Size: 2681 73 1663 1156 2573 Fragments arranged by size: 2681 2573 1663 1156 73 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8146 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4858 5877 6835 Size: 416 53 83 399 24 3441 1019 958 Cuts at: 6835 442 Size: 1753 Fragments arranged by size: 3441 1753 1019 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8146 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2669 3069 3278 3533 4377 4596 4813 4857 Size: 1430 400 209 255 844 219 217 44 Cuts at: 4857 4951 6806 1239 Size: 94 1855 2579 Fragments arranged by size: 2579 1855 1430 844 400 255 219 217 209 94 44 Bpu10I CC'TnA_GC Cuts at: 3684 3684 Size: 8146 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3773 6271 7136 7434 3773 Size: 2498 865 298 4485 Fragments arranged by size: 4485 2498 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6809 882 Size: 617 5310 2219 Fragments arranged by size: 5310 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4018 4675 5937 493 Size: 711 2814 657 1262 2702 Fragments arranged by size: 2814 2702 1262 711 657 BsaBI GATnn'nnATC Cuts at: 4266 8140 4266 Size: 3874 4272 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5953 6335 6970 275 Size: 53 83 186 936 4420 382 635 1451 Fragments arranged by size: 4420 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4776 6579 6977 7251 7398 7809 4776 Size: 1803 398 274 147 411 5113 Fragments arranged by size: 5113 1803 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2642 2672 3046 3076 3831 Size: 30 165 30 1239 30 374 30 755 Cuts at: 3831 3861 1178 Size: 30 5463 Fragments arranged by size: 5463 1239 755 374 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2240 3701 4595 1186 Size: 1054 1461 894 4737 Fragments arranged by size: 4737 1461 1054 894 BseYI C'CCAG_C Cuts at: 4888 5130 7349 7845 4888 Size: 242 2219 496 5189 Fragments arranged by size: 5189 2219 496 242 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5596 6357 7385 1540 Size: 275 186 3595 761 1028 2301 Fragments arranged by size: 3595 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2990 4471 6207 6292 7363 2990 Size: 1481 1736 85 1071 3773 Fragments arranged by size: 3773 1736 1481 1071 85 BsmI GAATG_Cn' Cuts at: 4033 5657 5750 4033 Size: 1624 93 6429 Fragments arranged by size: 6429 1624 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2222 2222 Size: 8146 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2990 3191 4471 4544 5415 5465 6207 Size: 399 1200 201 1280 73 871 50 742 Cuts at: 6207 6292 7363 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1280 1200 1071 871 742 399 201 85 73 50 BspEI T'CCGG_A Cuts at: 4776 7809 4776 Size: 3033 5113 BspHI T'CATG_A Cuts at: 4627 6035 7765 4627 Size: 1408 1730 5008 Fragments arranged by size: 5008 1730 1408 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2227 3667 5501 844 Size: 1383 1440 1834 3489 Fragments arranged by size: 3489 1834 1440 1383 BsrBI CCG'CTC Cuts at: 1394 1823 6033 1394 Size: 429 4210 3507 Fragments arranged by size: 4210 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4189 4264 6641 6815 65 Size: 1183 2941 75 2377 174 1396 Fragments arranged by size: 2941 2377 1396 1183 174 75 BsrFI r'CCGG_y Cuts at: 1700 1810 6790 6977 1700 Size: 110 4980 187 2869 Fragments arranged by size: 4980 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 5020 96 Size: 4924 3222 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8146 BssSI C'ACGA_G Cuts at: 6206 7218 6206 Size: 1012 7134 BstAPI GCAn_nnn'nTGC Cuts at: 794 4717 794 Size: 3923 4223 BstBI TT'CG_AA Cuts at: 5438 6950 5438 Size: 1512 6634 BstEII G'GTnAC_C Cuts at: 1184 3812 4754 1184 Size: 2628 942 4576 Fragments arranged by size: 4576 2628 942 BstXI CCAn_nnnn'nTGG Cuts at: 3184 3184 Size: 8146 BstYI r'GATC_y Cuts at: 1972 2308 2393 2531 4615 4773 5164 5476 5843 Size: 336 85 138 2084 158 391 312 367 Cuts at: 5843 6228 6245 7697 7783 7795 8141 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 2084 1977 1452 391 385 367 346 336 312 158 138 86 85 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6655 Fragments arranged by size: 6655 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2596 4764 487 Size: 683 56 1370 2168 3869 Fragments arranged by size: 3869 2168 1370 683 56 BtsI GCAGTG_nn' Cuts at: 3739 5662 6456 6476 3739 Size: 1923 794 20 5409 Fragments arranged by size: 5409 1923 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8146 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8111 DraI TTT'AAA Cuts at: 2106 3934 4369 4380 5450 2106 Size: 1828 435 11 1070 4802 Fragments arranged by size: 4802 1828 1070 435 11 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7153 809 Size: 507 5837 1802 Fragments arranged by size: 5837 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5593 6482 8111 Size: 54 1425 50 275 18 3763 889 1629 Cuts at: 8111 8 Size: 43 Fragments arranged by size: 3763 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5593 1537 Size: 275 3781 4090 Fragments arranged by size: 4090 3781 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2667 2941 2976 4132 4655 6076 1353 Size: 1314 274 35 1156 523 1421 3423 Fragments arranged by size: 3423 1421 1314 1156 523 274 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6733 7107 7253 387 Size: 1064 138 5144 374 146 1280 Fragments arranged by size: 5144 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2988 2988 Size: 8146 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2669 3069 3132 3278 3533 4113 4377 Size: 393 1037 400 63 146 255 580 264 Cuts at: 4377 4596 4813 4857 4951 6209 6806 7593 7861 Size: 219 217 44 94 1258 597 787 268 Cuts at: 7861 1239 Size: 1524 Fragments arranged by size: 1524 1258 1037 787 597 580 400 393 268 264 255 219 217 146 94 63 44 EcoO109I rG'GnC_Cy Cuts at: 3173 3953 4933 3173 Size: 780 980 6386 Fragments arranged by size: 6386 980 780 EcoRI G'AATT_C Cuts at: 4200 5455 5833 4200 Size: 1255 378 6513 Fragments arranged by size: 6513 1255 378 EcoRV GAT'ATC Cuts at: 4432 4531 4432 Size: 99 8047 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5231 5231 Size: 8146 FspI TGC'GCA Cuts at: 4809 6652 4809 Size: 1843 6303 HaeII r_GCGC'y Cuts at: 1678 7293 1678 Size: 5615 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2204 2236 2836 2868 3046 Size: 32 135 32 632 32 600 32 178 Cuts at: 3046 3078 3269 3301 3908 3940 4270 4302 4375 Size: 32 191 32 607 32 330 32 73 Cuts at: 4375 4407 4982 5014 5300 5332 6787 6819 6861 Size: 32 575 32 286 32 1455 32 42 Cuts at: 6861 6893 1373 Size: 32 2626 Fragments arranged by size: 2626 1455 632 607 600 575 330 286 191 178 135 73 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5490 5736 669 Size: 988 281 3552 246 3079 Fragments arranged by size: 3552 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3144 748 Size: 2396 5750 HpaI GTT'AAC Cuts at: 5736 5736 Size: 8146 KpnI G_GTAC'C Cuts at: 5471 5471 Size: 8146 MfeI C'AATT_G Cuts at: 5745 5745 Size: 8146 MluI A'CGCG_T Cuts at: 8022 8022 Size: 8146 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2679 7260 7444 1305 Size: 1374 4581 184 2007 Fragments arranged by size: 4581 2007 1374 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6324 Fragments arranged by size: 6324 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2667 3251 4673 6104 6463 6622 518 Size: 648 1501 584 1422 1431 359 159 2042 Fragments arranged by size: 2042 1501 1431 1422 648 584 359 159 MspA1I CmG'CkG Cuts at: 1606 2915 4574 5130 5562 6239 7387 7632 1606 Size: 1309 1659 556 432 677 1148 245 2120 Fragments arranged by size: 2120 1659 1309 1148 677 556 432 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6655 Fragments arranged by size: 6655 939 552 NdeI CA'TA_TG Cuts at: 387 4745 4947 387 Size: 4358 202 3586 Fragments arranged by size: 4358 3586 202 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8146 NotI GC'GGCC_GC Cuts at: 5593 5593 Size: 8146 NspI r_CATG'y Cuts at: 1132 1229 4148 4597 5504 7049 1132 Size: 97 2919 449 907 1545 2229 Fragments arranged by size: 2919 2229 1545 907 449 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8146 PciI A'CATG_T Cuts at: 4593 7045 4593 Size: 2452 5694 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8146 PfoI T'CCnGG_A Cuts at: 4656 4834 4656 Size: 178 7968 PmeI GTTT'AAAC Cuts at: 5450 5450 Size: 8146 PmlI CAC'GTG Cuts at: 4675 4675 Size: 8146 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6215 6247 7728 7760 6215 Size: 32 1481 32 6601 Fragments arranged by size: 6601 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3173 4933 3173 Size: 1760 6386 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8146 PsiI TTA'TAA Cuts at: 2994 5716 2994 Size: 2722 5424 PspOMI G'GGCC_C Cuts at: 4540 4540 Size: 8146 PstI C_TGCA'G Cuts at: 830 5498 830 Size: 4668 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8146 PvuII CAG'CTG Cuts at: 2915 4574 5130 2915 Size: 1659 556 5931 Fragments arranged by size: 5931 1659 556 SacI G_AGCT'C Cuts at: 2990 2990 Size: 8146 SalI G'TCGA_C Cuts at: 1936 5488 1936 Size: 3552 4594 SbfI CC_TGCA'GG Cuts at: 5498 5498 Size: 8146 ScaI AGT'ACT Cuts at: 2499 6394 8106 2499 Size: 3895 1712 2539 Fragments arranged by size: 3895 2539 1712 SexAI A'CCwGG_T Cuts at: 2287 2287 Size: 8146 SfcI C'TryA_G Cuts at: 826 1045 2848 4683 4905 5340 5494 6629 7310 Size: 219 1803 1835 222 435 154 1135 681 Cuts at: 7310 7501 826 Size: 191 1471 Fragments arranged by size: 1835 1803 1471 1135 681 435 222 219 191 154 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8146 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8146 SmaI CCC'GGG Cuts at: 1872 5473 1872 Size: 3601 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3788 6250 7151 7413 820 Size: 197 926 1845 2462 901 262 1553 Fragments arranged by size: 2462 1845 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5937 493 Size: 5444 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8146 SphI G_CATG'C Cuts at: 1132 5504 1132 Size: 4372 3774 SspI AAT'ATT Cuts at: 5 52 6070 5 Size: 47 6018 2081 Fragments arranged by size: 6018 2081 47 StuI AGG'CCT Cuts at: 1517 3231 1517 Size: 1714 6432 StyI C'CwwG_G Cuts at: 513 1065 2004 2855 2943 3177 3396 3538 5169 Size: 552 939 851 88 234 219 142 1631 Cuts at: 5169 5585 513 Size: 416 3074 Fragments arranged by size: 3074 1631 939 851 552 416 234 219 142 88 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2503 3751 6173 6190 6343 6528 6973 Size: 1255 923 1248 2422 17 153 185 445 Cuts at: 6973 325 Size: 1498 Fragments arranged by size: 2422 1498 1255 1248 923 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2497 5020 5053 Size: 274 80 33 51 1443 520 2523 33 Cuts at: 5053 6392 8104 96 Size: 1339 1712 138 Fragments arranged by size: 2523 1712 1443 1339 520 274 138 80 51 33 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8106 8138 8106 Size: 32 8114 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8146 XbaI T'CTAG_A Cuts at: 5249 5482 5249 Size: 233 7913 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2956 3985 2956 Size: 1029 7117 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8146 XmaI C'CCGG_G Cuts at: 1870 5471 1870 Size: 3601 4545 XmnI GAAnn'nnTTC Cuts at: 5434 6275 5434 Size: 841 7305 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5954 276 Size: 53 83 186 936 4420 2468 Fragments arranged by size: 4420 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI