(Circular) (Six-base) MAPSORT of: pFN21AB3037.seq Check: 3075 from: 1 to: 8155 pFN21AB3037 8155 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5965 278 Size: 53 83 186 936 4429 2468 Fragments arranged by size: 4429 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5498 1937 Size: 3561 4594 Acc65I G'GTAC_C Cuts at: 5476 5476 Size: 8155 AclI AA'CG_TT Cuts at: 1563 6282 6655 1563 Size: 4719 373 3063 Fragments arranged by size: 4719 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2271 2643 3945 4170 4454 4926 5150 6218 Size: 639 372 1302 225 284 472 224 1068 Cuts at: 6218 7602 7870 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1302 1068 639 472 372 284 268 225 224 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7958 AflIII A'CryG_T Cuts at: 1203 7054 8031 1203 Size: 5851 977 1327 Fragments arranged by size: 5851 1327 977 AgeI A'CCGG_T Cuts at: 3644 5265 6986 3644 Size: 1621 1721 4813 Fragments arranged by size: 4813 1721 1621 AhdI GACnn_n'nnGTC Cuts at: 6884 6884 Size: 8155 AleI CACnn'nnGTG Cuts at: 1166 2151 1166 Size: 985 7170 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4646 1184 Size: 3462 4693 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2504 2536 2582 2614 2504 Size: 32 46 32 8045 Fragments arranged by size: 8045 46 32 32 AlwNI CAG_nnn'CTG Cuts at: 3032 5898 7470 3032 Size: 2866 1572 3717 Fragments arranged by size: 3717 2866 1572 ApaLI G'TGCA_C Cuts at: 6212 7368 6212 Size: 1156 6999 ApoI r'AATT_y Cuts at: 1493 2381 2888 3127 3353 3476 3508 3873 4403 Size: 888 507 239 226 123 32 365 530 Cuts at: 4403 4523 4721 5375 5464 5690 5842 7951 1493 Size: 120 198 654 89 226 152 2109 1697 Fragments arranged by size: 2109 1697 888 654 530 507 365 239 226 226 198 152 123 120 89 32 AseI AT'TA_AT Cuts at: 160 1032 6709 160 Size: 872 5677 1606 Fragments arranged by size: 5677 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8155 AvaI C'yCGr_G Cuts at: 1870 1943 5480 1870 Size: 73 3537 4545 Fragments arranged by size: 4545 3537 73 BamHI G'GATC_C Cuts at: 5485 5852 5485 Size: 367 7788 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5476 6931 618 Size: 325 217 627 3689 1455 1842 Fragments arranged by size: 3689 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3772 5049 5253 5474 1391 Size: 2381 1277 204 221 4072 Fragments arranged by size: 4072 2381 1277 221 204 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2634 3459 3807 4331 4406 4917 928 Size: 971 735 825 348 524 75 511 4166 Fragments arranged by size: 4166 971 825 735 524 511 348 75 BbvCI CC'TCA_GC Cuts at: 5277 5277 Size: 8155 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6346 6380 1903 Size: 34 4409 34 3678 Fragments arranged by size: 4409 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2578 4477 6047 7257 2578 Size: 1899 1570 1210 3476 Fragments arranged by size: 3476 1899 1570 1210 BclI T'GATC_A Cuts at: 1548 2934 3993 4741 1548 Size: 1386 1059 748 4962 Fragments arranged by size: 4962 1386 1059 748 BfrBI ATG'CAT Cuts at: 2763 2763 Size: 8155 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6766 136 Size: 107 122 71 631 605 5094 1525 Fragments arranged by size: 5094 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2203 3661 4580 5125 8150 2203 Size: 1458 919 545 3025 2208 Fragments arranged by size: 3025 2208 1458 919 545 BlpI GC'TnA_GC Cuts at: 5572 5572 Size: 8155 Bme1580I G_kGCm'C Cuts at: 1790 6216 7372 1790 Size: 4426 1156 2573 Fragments arranged by size: 4426 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8155 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2969 5269 5886 Size: 416 53 83 399 24 1552 2300 617 Cuts at: 5886 6844 442 Size: 958 1753 Fragments arranged by size: 2300 1753 1552 958 617 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8155 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3969 4029 4503 5015 5198 6815 1239 Size: 2730 60 474 512 183 1617 2579 Fragments arranged by size: 2730 2579 1617 512 474 183 60 Bpu10I CC'TnA_GC Cuts at: 5247 5277 5247 Size: 30 8125 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3686 3795 6280 7145 7443 3686 Size: 109 2485 865 298 4398 Fragments arranged by size: 4398 2485 865 298 109 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3483 3569 4502 6818 882 Size: 617 1984 86 933 2316 2219 Fragments arranged by size: 2316 2219 1984 933 617 86 BsaAI yAC'GTr Cuts at: 493 1204 5946 493 Size: 711 4742 2702 Fragments arranged by size: 4742 2702 711 BsaBI GATnn'nnATC Cuts at: 2202 4740 8149 2202 Size: 2538 3409 2208 Fragments arranged by size: 3409 2538 2208 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5962 6344 6979 275 Size: 53 83 186 936 4429 382 635 1451 Fragments arranged by size: 4429 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3644 5265 6588 6986 7260 7407 7818 3644 Size: 1621 1323 398 274 147 411 3981 Fragments arranged by size: 3981 1621 1323 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4959 4989 1178 Size: 30 165 30 3556 30 4344 Fragments arranged by size: 4344 3556 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 5024 5294 1186 Size: 3838 270 4047 Fragments arranged by size: 4047 3838 270 BseYI C'CCAG_C Cuts at: 7358 7854 7358 Size: 496 7659 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2181 4851 5158 1163 Size: 573 445 2670 307 4160 Fragments arranged by size: 4160 2670 573 445 307 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5605 6366 7394 1540 Size: 275 186 3604 761 1028 2301 Fragments arranged by size: 3604 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 6216 6301 7372 6216 Size: 85 1071 6999 Fragments arranged by size: 6999 1071 85 BsmI GAATG_Cn' Cuts at: 3830 4114 5666 5759 3830 Size: 284 1552 93 6226 Fragments arranged by size: 6226 1552 284 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4962 5282 4962 Size: 320 7835 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3772 5049 5253 5474 6216 6301 7372 Size: 399 1982 1277 204 221 742 85 1071 Cuts at: 7372 1391 Size: 2174 Fragments arranged by size: 2174 1982 1277 1071 742 399 221 204 85 BspEI T'CCGG_A Cuts at: 7818 7818 Size: 8155 BspHI T'CATG_A Cuts at: 3319 4177 4387 6044 7774 3319 Size: 858 210 1657 1730 3700 Fragments arranged by size: 3700 1730 1657 858 210 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2114 5368 5510 844 Size: 1270 3254 142 3489 Fragments arranged by size: 3489 3254 1270 142 BsrBI CCG'CTC Cuts at: 1394 1823 6042 1394 Size: 429 4219 3507 Fragments arranged by size: 4219 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4353 6650 6824 65 Size: 1183 3105 2297 174 1396 Fragments arranged by size: 3105 2297 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2067 3644 5265 6799 6986 1700 Size: 110 257 1577 1621 1534 187 2869 Fragments arranged by size: 2869 1621 1577 1534 257 187 110 BsrGI T'GTAC_A Cuts at: 96 3100 96 Size: 3004 5151 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8155 BssSI C'ACGA_G Cuts at: 6215 7227 6215 Size: 1012 7143 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8155 BstBI TT'CG_AA Cuts at: 6959 6959 Size: 8155 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8155 BstXI CCAn_nnnn'nTGG Cuts at: 2234 2234 Size: 8155 BstYI r'GATC_y Cuts at: 1972 2203 2303 3661 4580 5125 5206 5485 5852 Size: 231 100 1358 919 545 81 279 367 Cuts at: 5852 6237 6254 7706 7792 7804 8150 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1358 919 545 385 367 346 279 231 100 86 81 17 12 Bsu36I CC'TnA_GG Cuts at: 3447 3447 Size: 8155 BtgI C'CryG_G Cuts at: 513 1065 2004 4133 4545 5034 513 Size: 552 939 2129 412 489 3634 Fragments arranged by size: 3634 2129 939 552 489 412 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7416 Fragments arranged by size: 7416 683 56 BtsI GCAGTG_nn' Cuts at: 5021 5671 6465 6485 5021 Size: 650 794 20 6691 Fragments arranged by size: 6691 794 650 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8155 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8120 DraI TTT'AAA Cuts at: 2733 3624 5459 2733 Size: 891 1835 5429 Fragments arranged by size: 5429 1835 891 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4345 7162 809 Size: 507 3029 2817 1802 Fragments arranged by size: 3029 2817 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2491 5602 6491 Size: 54 1425 50 275 18 661 3111 889 Cuts at: 6491 8120 8 Size: 1629 43 Fragments arranged by size: 3111 1629 1425 889 661 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5602 1537 Size: 275 3790 4090 Fragments arranged by size: 4090 3790 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3255 3477 3519 3691 3753 4650 5126 6085 Size: 1902 222 42 172 62 897 476 959 Cuts at: 6085 1353 Size: 3423 Fragments arranged by size: 3423 1902 959 897 476 222 172 62 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6742 7116 7262 387 Size: 1064 138 5153 374 146 1280 Fragments arranged by size: 5153 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2271 2643 3945 3969 4029 4170 4454 Size: 393 639 372 1302 24 60 141 284 Cuts at: 4454 4503 4926 5015 5150 5198 6218 6815 7602 Size: 49 423 89 135 48 1020 597 787 Cuts at: 7602 7870 1239 Size: 268 1524 Fragments arranged by size: 1524 1302 1020 787 639 597 423 393 372 284 268 141 135 89 60 49 48 24 EcoRI G'AATT_C Cuts at: 2888 4523 5464 5842 2888 Size: 1635 941 378 5201 Fragments arranged by size: 5201 1635 941 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2716 2716 Size: 8155 FspI TGC'GCA Cuts at: 6661 6661 Size: 8155 HaeII r_GCGC'y Cuts at: 1678 7302 1678 Size: 5624 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2195 2227 2337 2369 3446 Size: 32 135 32 623 32 110 32 1077 Cuts at: 3446 3478 3539 3571 3659 3691 4382 4414 4673 Size: 32 61 32 88 32 691 32 259 Cuts at: 4673 4705 6796 6828 6870 6902 1373 Size: 32 2091 32 42 32 2626 Fragments arranged by size: 2626 2091 1077 691 623 259 135 110 88 61 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3642 4576 5499 5745 669 Size: 988 281 1704 934 923 246 3079 Fragments arranged by size: 3079 1704 988 934 923 281 246 HindIII A'AGCT_T Cuts at: 748 5432 748 Size: 4684 3471 HpaI GTT'AAC Cuts at: 5745 5745 Size: 8155 KpnI G_GTAC'C Cuts at: 5480 5480 Size: 8155 MfeI C'AATT_G Cuts at: 5754 5754 Size: 8155 MluI A'CGCG_T Cuts at: 8031 8031 Size: 8155 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7269 7453 1305 Size: 5964 184 2007 Fragments arranged by size: 5964 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2493 10 Size: 54 1425 343 661 5672 Fragments arranged by size: 5672 1425 661 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2151 2180 2232 2693 3395 4358 4389 Size: 648 985 29 52 461 702 963 31 Cuts at: 4389 4392 4704 4958 6113 6472 6631 518 Size: 3 312 254 1155 359 159 2042 Fragments arranged by size: 2042 1155 985 963 702 648 461 359 312 254 159 52 31 29 3 MspA1I CmG'CkG Cuts at: 1606 4950 5099 5276 5571 6248 7396 7641 1606 Size: 3344 149 177 295 677 1148 245 2120 Fragments arranged by size: 3344 2120 1148 677 295 245 177 149 NcoI C'CATG_G Cuts at: 513 1065 2004 5034 513 Size: 552 939 3030 3634 Fragments arranged by size: 3634 3030 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8155 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8155 NotI GC'GGCC_GC Cuts at: 5602 5602 Size: 8155 NsiI A_TGCA'T Cuts at: 2765 2765 Size: 8155 NspI r_CATG'y Cuts at: 1132 1229 3368 5513 7058 1132 Size: 97 2139 2145 1545 2229 Fragments arranged by size: 2229 2145 2139 1545 97 PasI CC'CwG_GG Cuts at: 1348 2289 1348 Size: 941 7214 PciI A'CATG_T Cuts at: 7054 7054 Size: 8155 PflMI CCAn_nnn'nTGG Cuts at: 1108 2525 2981 1108 Size: 1417 456 6282 Fragments arranged by size: 6282 1417 456 PfoI T'CCnGG_A Cuts at: 3786 5325 3786 Size: 1539 6616 PmeI GTTT'AAAC Cuts at: 5459 5459 Size: 8155 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4928 4960 6224 6256 7737 7769 4928 Size: 32 1264 32 1481 32 5314 Fragments arranged by size: 5314 1481 1264 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8155 PsiI TTA'TAA Cuts at: 2499 5725 2499 Size: 3226 4929 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2070 2102 2070 Size: 32 8123 PstI C_TGCA'G Cuts at: 830 4315 4436 5507 830 Size: 3485 121 1071 3478 Fragments arranged by size: 3485 3478 1071 121 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8155 PvuII CAG'CTG Cuts at: 5099 5099 Size: 8155 SalI G'TCGA_C Cuts at: 1936 5497 1936 Size: 3561 4594 SbfI CC_TGCA'GG Cuts at: 5507 5507 Size: 8155 ScaI AGT'ACT Cuts at: 2035 5000 6403 8115 2035 Size: 2965 1403 1712 2075 Fragments arranged by size: 2965 2075 1712 1403 SexAI A'CCwGG_T Cuts at: 2852 2852 Size: 8155 SfcI C'TryA_G Cuts at: 826 1045 2047 3878 3922 4168 4311 4432 5503 Size: 219 1002 1831 44 246 143 121 1071 Cuts at: 5503 6638 7319 7510 826 Size: 1135 681 191 1471 Fragments arranged by size: 1831 1471 1135 1071 1002 681 246 219 191 143 121 44 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8155 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8155 SmaI CCC'GGG Cuts at: 1872 5482 1872 Size: 3610 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3665 3810 6259 7160 7422 820 Size: 197 926 1722 145 2449 901 262 1553 Fragments arranged by size: 2449 1722 1553 926 901 262 197 145 SnaBI TAC'GTA Cuts at: 493 5946 493 Size: 5453 2702 SpeI A'CTAG_T Cuts at: 152 3817 152 Size: 3665 4490 SphI G_CATG'C Cuts at: 1132 5513 1132 Size: 4381 3774 SspI AAT'ATT Cuts at: 5 52 4563 5400 6079 5 Size: 47 4511 837 679 2081 Fragments arranged by size: 4511 2081 837 679 47 StuI AGG'CCT Cuts at: 1517 2273 1517 Size: 756 7399 StyI C'CwwG_G Cuts at: 513 1065 2004 5034 5594 513 Size: 552 939 3030 560 3074 Fragments arranged by size: 3074 3030 939 560 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3706 6182 6199 6352 6537 6982 325 Size: 1255 2126 2476 17 153 185 445 1498 Fragments arranged by size: 2476 2126 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2033 3100 4975 Size: 274 80 33 51 1443 56 1067 1875 Cuts at: 4975 4998 6401 8113 96 Size: 23 1403 1712 138 Fragments arranged by size: 1875 1712 1443 1403 1067 274 138 80 56 51 33 23 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8115 8147 8115 Size: 32 8123 Tth111I GACn'n_nGTC Cuts at: 1316 3315 1316 Size: 1999 6156 XbaI T'CTAG_A Cuts at: 2917 4441 5491 2917 Size: 1524 1050 5581 Fragments arranged by size: 5581 1524 1050 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4990 4990 Size: 8155 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8155 XmaI C'CCGG_G Cuts at: 1870 5480 1870 Size: 3610 4545 XmnI GAAnn'nnTTC Cuts at: 6284 6284 Size: 8155 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5963 276 Size: 53 83 186 936 4429 2468 Fragments arranged by size: 4429 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI