Restriction map for
pFN21AB3030
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AhdI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BamHI BbeI BbrPI BbvCI BcgI
BfrI BlnI BsaHI BsiWI BspHI
BspLU11I BspMI BssHII BssSI Bst1107I
BstBI BstZ17I CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI FseI Hin1I HindIII HpaI
KasI KpnI KspI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SbfI ScaI SexAI SfiI SfoI
SfuI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI XbaI XcmI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 1746 CGCG CG^CG
AflIII 1544 ACGTGT A^C[GA][CT]GT
AgeI 991 ACCGGT A^CCGGT
Alw44I 2466 GTGCAC G^TGCAC
AlwNI 1447 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 343 CCTGAGG CC^T[ACGT]AGG
ApaLI 2466 GTGCAC G^TGCAC
AspI 1969 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 2531 GGATAC GTATCC(6/5)
BsaAI 1543 TACGTG [CT]AC^GT[GA]
BsaBI 762 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 993 CGGTCG CG[GA][CT]^CG
BsmBI 548 GAGACG CGTCTC(1/5)
BsmI 394 GAATGC GAATGC(1/-1)
Bsp1407I 899 TGTACA T^GTACA
BspDI 2275 ATCGAT AT^CGAT
BsrGI 899 TGTACA T^GTACA
BstEII 1899 GGTGACC G^GT[ACGT]ACC
BstPI 1899 GGTGACC G^GT[ACGT]ACC
BstUI 1746 CGCG CG^CG
Bsu36I 343 CCTGAGG CC^T[ACGT]AGG
ClaI 2275 ATCGAT AT^CGAT
Eco81I 343 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1899 GGTGACC G^GT[ACGT]ACC
EcoRV 598 GATATC GAT^ATC
EcoT22I 2623 ATGCAT ATGCA^T
HaeII 2703 GGCGCT [GA]GCGC^[CT]
MamI 762 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 2456 TTAA T^TAA
MvnI 1746 CGCG CG^CG
NcoI 1361 CCATGG C^CATGG
NsiI 2623 ATGCAT ATGCA^T
NspI 83 ACATGC [GA]CATG^[CT]
PaeR7I 1756 CTCGAG C^TCGAG
PflFI 1969 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 991 ACCGGT A^CCGGT
SalI 1234 GTCGAC G^TCGAC
SapI 2298 GCTCTTC GCTCTTC(1/4)
SgrAI 1924 CACCGGCG C[GA]^CCGG[CT]G
SspBI 899 TGTACA T^GTACA
Tru9I 2456 TTAA T^TAA
Tth111I 1969 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1756 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 566 TCCGGA T^CCGGA
1046 TCCGGA
AcsI 1608 AAATTT [GA]^AATT[CT]
2170 GAATTT
ApoI 1608 AAATTT [GA]^AATT[CT]
2170 GAATTT
AviII 1156 TGCGCA TGC^GCA
2069 TGCGCA
BalI 938 TGGCCA TGG^CCA
1106 TGGCCA
BbsI 1413 GAAGAC GAAGAC(2/6)
2002 GAAGAC
BclI 887 TGATCA T^GATCA
2231 TGATCA
BfaI 487 CTAG C^TAG
2152 CTAG
BglI 1162 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2479 GCCCGGCGGGC
BlpI 969 GCTCAGC GC^T[ACGT]AGC
1434 GCTGAGC
Bpu1102I 969 GCTCAGC GC^T[ACGT]AGC
1434 GCTGAGC
BpuAI 1413 GAAGAC GAAGAC(2/6)
2002 GAAGAC
BseAI 566 TCCGGA T^CCGGA
1046 TCCGGA
BsgI 55 GTGCAG GTGCAG(16/14)
1355 GTGCAG
BspEI 566 TCCGGA T^CCGGA
1046 TCCGGA
BstAPI 701 GCAACGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2045 GCAATGCCTGC
BstXI 530 CCAGTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2493 CCAGCTCTGTGG
CelII 969 GCTCAGC GC^T[ACGT]AGC
1434 GCTGAGC
DrdI 1237 GACGGTTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1726 GACCACATTGTC
DsaI 1361 CCATGG C^C[GA][CT]GG
2544 CCGTGG
EcoNI 197 CCTTTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1905 CCTGCTGAAGG
FbaI 887 TGATCA T^GATCA
2231 TGATCA
FspI 1156 TGCGCA TGC^GCA
2069 TGCGCA
HgaI 2554 GACGC GACGC(5/10)
1275 GCGTC
HincII 64 GTTGAC GT[CT]^[GA]AC
1234 GTCGAC
HindII 64 GTTGAC GT[CT]^[GA]AC
1234 GTCGAC
MaeI 487 CTAG C^TAG
2152 CTAG
MluNI 938 TGGCCA TGG^CCA
1106 TGGCCA
MroI 566 TCCGGA T^CCGGA
1046 TCCGGA
MscI 938 TGGCCA TGG^CCA
1106 TGGCCA
PflMI 306 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1446 CCAGCTGCTGG
PleI 1089 GACTC GAGTC(4/5)
1506 GACTC
SacI 37 GAGCTC GAGCT^C
1717 GAGCTC
SmaI 861 CCCGGG CCC^GGG
2205 CCCGGG
TfiI 324 GAATC G^A[AT]TC
521 GATTC
Tsp509I 1609 AATT ^AATT
2171 AATT
Van91I 306 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1446 CCAGCTGCTGG
XmaCI 861 CCCGGG C^CCGGG
2205 CCCGGG
XmaI 861 CCCGGG C^CCGGG
2205 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BglII BmyI BpmI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII