Restriction map for
pFN21AB3021
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BcgI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI
BstEII BstPI BstXI CelII Cfr10I
ClaI CpoI DrdI Eam1105I Eco47III
EcoNI EcoO65I EcoT22I FauI FseI
FspI HaeII HgaI Hin1I HindIII
KasI KpnI MroI MslI NaeI
NarI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1241 GTATAC GT^[AC][GT]AC
Alw44I 779 GTGCAC G^TGCAC
AocI 85 CCTGAGG CC^T[ACGT]AGG
ApaLI 779 GTGCAC G^TGCAC
Asp700I 1999 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1484 TGGCCA TGG^CCA
BanI 63 GGCACC G^G[CT][GA]CC
BbsI 2275 GTCTTC GAAGAC(2/6)
BbvCI 1898 CCTCAGC CCTCAGC(-5/-2)
BpuAI 2275 GTCTTC GAAGAC(2/6)
BsaBI 1810 GATATGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1218 CTGCAC GTGCAG(16/14)
BsmI 2729 GAATGC GAATGC(1/-1)
BspLU11I 502 ACATGT A^CATGT
BspMI 122 ACCTGC ACCTGC(4/8)
Bst1107I 1241 GTATAC GTA^TAC
BstAPI 2055 GCACACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 252 GGATG GGATG(2/0)
BstZ17I 1241 GTATAC GTA^TAC
Bsu36I 85 CCTGAGG CC^T[ACGT]AGG
CfoI 1466 GCGC GCG^C
DraIII 1991 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 26 CGGCCG C^GGCCG
EclXI 26 CGGCCG C^GGCCG
Eco52I 26 CGGCCG C^GGCCG
Eco81I 85 CCTGAGG CC^T[ACGT]AGG
EcoRI 900 GAATTC G^AATTC
EcoRV 540 GATATC GAT^ATC
EcoT14I 1543 CCATGG C^C[AT][AT]GG
FokI 252 GGATG GGATG(9/13)
HhaI 1466 GCGC GCG^C
HinP1I 1466 GCGC G^CGC
HpaI 225 GTTAAC GTT^AAC
KspI 50 CCGCGG CCGC^GG
MaeII 2220 ACGT A^CGT
MamI 1810 GATATGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2501 CAATTG C^AATTG
MluI 1858 ACGCGT A^CGCGT
MluNI 1484 TGGCCA TGG^CCA
MscI 1484 TGGCCA TGG^CCA
MunI 2501 CAATTG C^AATTG
NcoI 1543 CCATGG C^CATGG
NheI 1968 GCTAGC G^CTAGC
NspI 502 ACATGT [GA]CATG^[CT]
PvuII 722 CAGCTG CAG^CTG
SacII 50 CCGCGG CCGC^GG
SfaNI 994 GATGC GCATC(5/9)
SspI 2299 AATATT AAT^ATT
StyI 1543 CCATGG C^C[AT][AT]GG
Tsp45I 2337 GTCAC ^GT[GC]AC
XbaI 990 TCTAGA T^CTAGA
XmnI 1999 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccII 51 CGCG CG^CG
1859 CGCG
AfaI 422 GTAC GT^AC
1502 GTAC
AlwNI 2284 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
2695 CAGAAACTG
AseI 736 ATTAAT AT^TAAT
1152 ATTAAT
AsnI 736 ATTAAT AT^TAAT
1152 ATTAAT
BciVI 1267 GGATAC GTATCC(6/5)
2760 GGATAC
BcnI 60 CCCGG CC^[GC]GG
114 CCCGG
BglII 987 AGATCT A^GATCT
1831 AGATCT
BseRI 88 GAGGAG GAGGAG(10/8)
2239 GAGGAG
BsiEI 26 CGGCCG CG[GA][CT]^CG
2565 CGACCG
BsmFI 1302 GGGAC GGGAC(10/14)
2518 GGGAC
BspHI 1014 TCATGA T^CATGA
2081 TCATGA
BstUI 51 CGCG CG^CG
1859 CGCG
Cac8I 1462 GCGTGC GC[ACGT]^[ACGT]GC
1968 GCTAGC
DsaI 50 CCGCGG C^C[GA][CT]GG
1543 CCATGG
EaeI 26 CGGCCG [CT]^GGCC[GA]
1484 TGGCCA
HincII 225 GTTAAC GT[CT]^[GA]AC
322 GTTGAC
HindII 225 GTTAAC GT[CT]^[GA]AC
322 GTTGAC
MvnI 51 CGCG CG^CG
1859 CGCG
NciI 60 CCCGG CC^[GC]GG
114 CCCGG
NdeI 1447 CATATG CA^TATG
2130 CATATG
PpuMI 92 AGGACCC [GA]G^G[AT]CC[CT]
119 AGGACCT
PshBI 736 ATTAAT AT^TAAT
1152 ATTAAT
PstI 1802 CTGCAG CTGCA^G
2062 CTGCAG
RcaI 1014 TCATGA T^CATGA
2081 TCATGA
RsaI 422 GTAC GT^AC
1502 GTAC
SmlI 139 CTTGAG C^T[CT][GA]AG
1471 CTTGAG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AflIII AluI AlwI
ApoI AspHI AvaII BbvI BclI
BfaI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstNI BstYI Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII FbaI Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfcI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII