(Circular) (Six-base) MAPSORT of: pFN21AB3021.seq Check: 4631 from: 1 to: 7489 pFN21AB3021 7489 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5299 278 Size: 53 83 186 936 3763 2468 Fragments arranged by size: 3763 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3247 4832 1937 Size: 1310 1585 4594 Fragments arranged by size: 4594 1585 1310 Acc65I G'GTAC_C Cuts at: 4810 4810 Size: 7489 AclI AA'CG_TT Cuts at: 1563 5616 5989 1563 Size: 4053 373 3063 Fragments arranged by size: 4053 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2473 3017 3177 4451 4614 5552 6936 7204 Size: 841 544 160 1274 163 938 1384 268 Cuts at: 7204 1632 Size: 1917 Fragments arranged by size: 1917 1384 1274 938 841 544 268 163 160 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7292 AflIII A'CryG_T Cuts at: 1203 2507 3863 4225 6388 7365 1203 Size: 1304 1356 362 2163 977 1327 Fragments arranged by size: 2163 1356 1327 1304 977 362 AgeI A'CCGG_T Cuts at: 6320 6320 Size: 7489 AhdI GACnn_n'nnGTC Cuts at: 6218 6218 Size: 7489 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7489 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7489 AlwNI CAG_nnn'CTG Cuts at: 4294 4705 5232 6804 4294 Size: 411 527 1572 4979 Fragments arranged by size: 4979 1572 527 411 ApaLI G'TGCA_C Cuts at: 2784 5546 6702 2784 Size: 2762 1156 3571 Fragments arranged by size: 3571 2762 1156 ApoI r'AATT_y Cuts at: 1493 2393 2560 2887 2905 2973 3172 3290 3418 Size: 900 167 327 18 68 199 118 128 Cuts at: 3418 3871 4163 4446 4691 4798 5024 5176 7285 Size: 453 292 283 245 107 226 152 2109 Cuts at: 7285 1493 Size: 1697 Fragments arranged by size: 2109 1697 900 453 327 292 283 245 226 199 167 152 128 118 107 68 18 AseI AT'TA_AT Cuts at: 160 1032 2742 3158 6043 160 Size: 872 1710 416 2885 1606 Fragments arranged by size: 2885 1710 1606 872 416 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7489 AvaI C'yCGr_G Cuts at: 1870 1943 4814 1870 Size: 73 2871 4545 Fragments arranged by size: 4545 2871 73 BamHI G'GATC_C Cuts at: 4819 5186 4819 Size: 367 7122 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2068 4810 6265 618 Size: 325 217 627 281 2742 1455 1842 Fragments arranged by size: 2742 1842 1455 627 325 281 217 BanII G_rGCy'C Cuts at: 1391 4808 1391 Size: 3417 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4273 928 Size: 971 2374 4144 Fragments arranged by size: 4144 2374 971 BbvCI CC'TCA_GC Cuts at: 3904 3904 Size: 7489 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5680 5714 1903 Size: 34 50 34 3659 34 3678 Fragments arranged by size: 3678 3659 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3266 4759 5381 6591 3266 Size: 1493 622 1210 4164 Fragments arranged by size: 4164 1493 1210 622 BclI T'GATC_A Cuts at: 1548 3184 3754 4233 1548 Size: 1636 570 479 4804 Fragments arranged by size: 4804 1636 570 479 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6100 136 Size: 107 122 71 631 605 4428 1525 Fragments arranged by size: 4428 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2992 3836 7484 2992 Size: 844 3648 2997 Fragments arranged by size: 3648 2997 844 BlpI GC'TnA_GC Cuts at: 4906 4906 Size: 7489 Bme1580I G_kGCm'C Cuts at: 1790 2788 5550 6706 1790 Size: 998 2762 1156 2573 Fragments arranged by size: 2762 2573 1156 998 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7489 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5220 6178 442 Size: 416 53 83 399 24 3803 958 1753 Fragments arranged by size: 3803 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3977 1056 Size: 2921 4568 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3108 3776 4425 6149 1239 Size: 1869 668 649 1724 2579 Fragments arranged by size: 2579 1869 1724 668 649 Bpu10I CC'TnA_GC Cuts at: 3904 3904 Size: 7489 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2165 3497 5614 6479 6777 2165 Size: 1332 2117 865 298 2877 Fragments arranged by size: 2877 2117 1332 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6152 882 Size: 617 4653 2219 Fragments arranged by size: 4653 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5280 493 Size: 711 4076 2702 Fragments arranged by size: 4076 2702 711 BsaBI GATnn'nnATC Cuts at: 3819 7483 3819 Size: 3664 3825 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5296 5678 6313 275 Size: 53 83 186 936 3763 382 635 1451 Fragments arranged by size: 3763 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5922 6320 6594 6741 7152 5922 Size: 398 274 147 411 6259 Fragments arranged by size: 6259 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7264 Fragments arranged by size: 7264 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 4259 1186 Size: 922 2151 4416 Fragments arranged by size: 4416 2151 922 BseYI C'CCAG_C Cuts at: 2534 6692 7188 2534 Size: 4158 496 2835 Fragments arranged by size: 4158 2835 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3208 1163 Size: 573 1472 5444 Fragments arranged by size: 5444 1472 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 4573 4939 5700 6728 1540 Size: 275 186 33 2539 366 761 1028 2301 Fragments arranged by size: 2539 2301 1028 761 366 275 186 33 BsiHKAI G_wGCw'C Cuts at: 2788 4023 4581 5550 5635 6706 2788 Size: 1235 558 969 85 1071 3571 Fragments arranged by size: 3571 1235 1071 969 558 85 BsmI GAATG_Cn' Cuts at: 4740 5000 5093 4740 Size: 260 93 7136 Fragments arranged by size: 7136 260 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2788 4023 4581 4808 5550 5635 6706 Size: 399 998 1235 558 227 742 85 1071 Cuts at: 6706 1391 Size: 2174 Fragments arranged by size: 2174 1235 1071 998 742 558 399 227 85 BspEI T'CCGG_A Cuts at: 7152 7152 Size: 7489 BspHI T'CATG_A Cuts at: 3019 4086 5378 7108 3019 Size: 1067 1292 1730 3400 Fragments arranged by size: 3400 1730 1292 1067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2136 4844 844 Size: 1292 2708 3489 Fragments arranged by size: 3489 2708 1292 BsrBI CCG'CTC Cuts at: 1394 1823 5376 1394 Size: 429 3553 3507 Fragments arranged by size: 3553 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5984 6158 65 Size: 1183 4736 174 1396 Fragments arranged by size: 4736 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6133 6320 1700 Size: 110 4323 187 2869 Fragments arranged by size: 4323 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7489 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7489 BssSI C'ACGA_G Cuts at: 5549 6561 5549 Size: 1012 6477 BstAPI GCAn_nnn'nTGC Cuts at: 794 4066 794 Size: 3272 4217 BstBI TT'CG_AA Cuts at: 6293 6293 Size: 7489 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7489 BstYI r'GATC_y Cuts at: 1972 2992 3836 4192 4819 5186 5571 5588 7040 Size: 1020 844 356 627 367 385 17 1452 Cuts at: 7040 7126 7138 7484 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1020 844 627 385 367 356 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3248 3248 Size: 7489 Bsu36I CC'TnA_GG Cuts at: 2091 2091 Size: 7489 BtgI C'CryG_G Cuts at: 513 1065 2055 3548 513 Size: 552 990 1493 4454 Fragments arranged by size: 4454 1493 990 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6750 Fragments arranged by size: 6750 683 56 BtsI GCAGTG_nn' Cuts at: 3804 5005 5799 5819 3804 Size: 1201 794 20 5474 Fragments arranged by size: 5474 1201 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7489 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7454 DraI TTT'AAA Cuts at: 3171 3283 3573 4793 3171 Size: 112 290 1220 5867 Fragments arranged by size: 5867 1220 290 112 DraIII CAC_nnn'GTG Cuts at: 4001 4001 Size: 7489 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6496 809 Size: 507 5180 1802 Fragments arranged by size: 5180 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 3489 4936 Size: 54 1425 50 275 18 201 1458 1447 Cuts at: 4936 5825 7454 8 Size: 889 1629 43 Fragments arranged by size: 1629 1458 1447 1425 889 275 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2031 4936 1537 Size: 275 219 2905 4090 Fragments arranged by size: 4090 2905 275 219 EarI CTCTTCn'nnn_ Cuts at: 1353 2492 3036 3090 4236 4427 4776 5419 1353 Size: 1139 544 54 1146 191 349 643 3423 Fragments arranged by size: 3423 1146 1139 643 544 349 191 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6076 6450 6596 387 Size: 1064 138 4487 374 146 1280 Fragments arranged by size: 4487 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2473 3017 3108 3177 3776 4425 4451 Size: 393 841 544 91 69 599 649 26 Cuts at: 4451 4614 5552 6149 6936 7204 1239 Size: 163 938 597 787 268 1524 Fragments arranged by size: 1524 938 841 787 649 599 597 544 393 268 163 91 69 26 EcoO109I rG'GnC_Cy Cuts at: 2098 2125 4329 2098 Size: 27 2204 5258 Fragments arranged by size: 5258 2204 27 EcoRI G'AATT_C Cuts at: 2905 4798 5176 2905 Size: 1893 378 5218 Fragments arranged by size: 5218 1893 378 EcoRV GAT'ATC Cuts at: 2547 2547 Size: 7489 FspI TGC'GCA Cuts at: 5995 5995 Size: 7489 HaeII r_GCGC'y Cuts at: 1678 6636 1678 Size: 4958 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2191 2223 2327 2359 4398 Size: 32 135 32 619 32 104 32 2039 Cuts at: 4398 4430 4479 4511 4708 4740 6130 6162 6204 Size: 32 49 32 197 32 1390 32 42 Cuts at: 6204 6236 1373 Size: 32 2626 Fragments arranged by size: 2626 2039 1390 619 197 135 104 49 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2232 2329 4833 5079 669 Size: 988 281 294 97 2504 246 3079 Fragments arranged by size: 3079 2504 988 294 281 246 97 HindIII A'AGCT_T Cuts at: 748 748 Size: 7489 HpaI GTT'AAC Cuts at: 2232 5079 2232 Size: 2847 4642 KpnI G_GTAC'C Cuts at: 4814 4814 Size: 7489 MfeI C'AATT_G Cuts at: 4506 5088 4506 Size: 582 6907 MluI A'CGCG_T Cuts at: 3863 7365 3863 Size: 3502 3987 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3175 6603 6787 1305 Size: 1870 3428 184 2007 Fragments arranged by size: 3428 2007 1870 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3491 10 Size: 54 1425 343 1659 4008 Fragments arranged by size: 4008 1659 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5447 5806 5965 518 Size: 648 4281 359 159 2042 Fragments arranged by size: 4281 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2048 2057 2115 2729 4905 5582 6730 6975 Size: 442 9 58 614 2176 677 1148 245 Cuts at: 6975 1606 Size: 2120 Fragments arranged by size: 2176 2120 1148 677 614 442 245 58 9 NcoI C'CATG_G Cuts at: 513 1065 3548 513 Size: 552 2483 4454 Fragments arranged by size: 4454 2483 552 NdeI CA'TA_TG Cuts at: 387 3453 4136 387 Size: 3066 683 3740 Fragments arranged by size: 3740 3066 683 NheI G'CTAG_C Cuts at: 1052 3973 1052 Size: 2921 4568 NotI GC'GGCC_GC Cuts at: 4936 4936 Size: 7489 NspI r_CATG'y Cuts at: 1132 1229 2511 4847 6392 1132 Size: 97 1282 2336 1545 2229 Fragments arranged by size: 2336 2229 1545 1282 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7489 PciI A'CATG_T Cuts at: 2507 6388 2507 Size: 3881 3608 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7489 PfoI T'CCnGG_A Cuts at: 4402 4402 Size: 7489 PmeI GTTT'AAAC Cuts at: 4793 4793 Size: 7489 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2330 2362 5558 5590 7071 7103 2330 Size: 32 3196 32 1481 32 2716 Fragments arranged by size: 3196 2716 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2098 2125 2098 Size: 27 7462 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7489 PsiI TTA'TAA Cuts at: 3957 5059 3957 Size: 1102 6387 PstI C_TGCA'G Cuts at: 830 3811 4071 4841 830 Size: 2981 260 770 3478 Fragments arranged by size: 3478 2981 770 260 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7489 PvuII CAG'CTG Cuts at: 2729 2729 Size: 7489 SacII CC_GC'GG Cuts at: 2058 2058 Size: 7489 SalI G'TCGA_C Cuts at: 1936 4831 1936 Size: 2895 4594 SbfI CC_TGCA'GG Cuts at: 4841 4841 Size: 7489 ScaI AGT'ACT Cuts at: 5737 7449 5737 Size: 1712 5777 SfcI C'TryA_G Cuts at: 826 1045 2363 3045 3807 4067 4837 5972 6653 Size: 219 1318 682 762 260 770 1135 681 Cuts at: 6653 6844 826 Size: 191 1471 Fragments arranged by size: 1471 1318 1135 770 762 682 681 260 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7489 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7489 SmaI CCC'GGG Cuts at: 1872 4816 1872 Size: 2944 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2144 3476 5593 6494 6756 820 Size: 197 926 201 1332 2117 901 262 1553 Fragments arranged by size: 2117 1553 1332 926 901 262 201 197 SnaBI TAC'GTA Cuts at: 493 5280 493 Size: 4787 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7489 SphI G_CATG'C Cuts at: 1132 4847 1132 Size: 3715 3774 SspI AAT'ATT Cuts at: 5 52 4306 5413 5 Size: 47 4254 1107 2081 Fragments arranged by size: 4254 2081 1107 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7489 StyI C'CwwG_G Cuts at: 513 1065 3548 4928 513 Size: 552 2483 1380 3074 Fragments arranged by size: 3074 2483 1380 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2776 3753 4038 4267 5516 5533 5686 Size: 1255 1196 977 285 229 1249 17 153 Cuts at: 5686 5871 6316 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1249 1196 977 445 285 229 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3506 5735 7447 Size: 274 80 33 51 1443 1529 2229 1712 Cuts at: 7447 96 Size: 138 Fragments arranged by size: 2229 1712 1529 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3217 3249 7449 7481 3217 Size: 32 4200 32 3225 Fragments arranged by size: 4200 3225 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7489 XbaI T'CTAG_A Cuts at: 2995 4825 2995 Size: 1830 5659 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7489 XmaI C'CCGG_G Cuts at: 1870 4814 1870 Size: 2944 4545 XmnI GAAnn'nnTTC Cuts at: 4008 5618 4008 Size: 1610 5879 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5297 276 Size: 53 83 186 936 3763 2468 Fragments arranged by size: 3763 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI